Remarkable progress in correlative light and electron cryo-microscopy(cryo-CLEM) has been made in the past decade. A crucial component for cryo-CLEM is a dedicated cryo-fluorescence microscope(cryo-FM). Here, we descr...Remarkable progress in correlative light and electron cryo-microscopy(cryo-CLEM) has been made in the past decade. A crucial component for cryo-CLEM is a dedicated cryo-fluorescence microscope(cryo-FM). Here, we describe an ultra-stable superresolution cryo-FM that exhibits excellent thermal and mechanical stability. The temperature fluctuations in 10 h are less than0.06 K, and the mechanical drift over 5 h is less than 200 nm in three dimensions. We have demonstrated the super-resolution imaging capability of this system(average single molecule localization accuracy of ～13.0 nm). The results suggest that our system is particularly suitable for long-term observations, such as single molecule localization microscopy(SMLM) and cryogenic super-resolution correlative light and electron microscopy(csCLEM).展开更多
Dear Editor,Insulin is important for body metabolism regulation and glucose homeostasis,and its dysregulation often leads to metabolic syndrome(MS)and diabetes.Insulin is normally stored in large dense-core vesicles(L...Dear Editor,Insulin is important for body metabolism regulation and glucose homeostasis,and its dysregulation often leads to metabolic syndrome(MS)and diabetes.Insulin is normally stored in large dense-core vesicles(LDCVs)in pancreatic beta cells,and significant reductions in the number,size,gray level and density of insulin granules confer diabetes both in mice(Xue et al.,2012)and humans(Masini et al.,2012).展开更多
基金supported by the National Key R&D Program of China (2016YFA0500203, 2016YFA0502400, 2017YFA0504700, 2017YFA0505300)the National Natural Science Foundation of China (31661143041, 31127901)Joint Program between Chinese Academy of Sciences and Peking University
文摘Remarkable progress in correlative light and electron cryo-microscopy(cryo-CLEM) has been made in the past decade. A crucial component for cryo-CLEM is a dedicated cryo-fluorescence microscope(cryo-FM). Here, we describe an ultra-stable superresolution cryo-FM that exhibits excellent thermal and mechanical stability. The temperature fluctuations in 10 h are less than0.06 K, and the mechanical drift over 5 h is less than 200 nm in three dimensions. We have demonstrated the super-resolution imaging capability of this system(average single molecule localization accuracy of ～13.0 nm). The results suggest that our system is particularly suitable for long-term observations, such as single molecule localization microscopy(SMLM) and cryogenic super-resolution correlative light and electron microscopy(csCLEM).
基金This work was supported by grants from the National Key R&D Program of China(Grant Nos.2017YFA0504700 and 2016YFA0500400)the National Natural Science Foundation of China(Grant Nos.31570839,31661143041,61472395,31327901,31521062 and 31730054)+1 种基金the Beijing Natural Science Foundation(L172003)Joint Program between Chinese Academy of Sciences and Peking University.
文摘Dear Editor,Insulin is important for body metabolism regulation and glucose homeostasis,and its dysregulation often leads to metabolic syndrome(MS)and diabetes.Insulin is normally stored in large dense-core vesicles(LDCVs)in pancreatic beta cells,and significant reductions in the number,size,gray level and density of insulin granules confer diabetes both in mice(Xue et al.,2012)and humans(Masini et al.,2012).
