Two parallel carbon-membrane aerated biofilm reactors were operated at well-defined conditions to investigate the effect of substrate COD/N ratios on the performance and microbial community structure of the bioreactor...Two parallel carbon-membrane aerated biofilm reactors were operated at well-defined conditions to investigate the effect of substrate COD/N ratios on the performance and microbial community structure of the bioreactor. Results showed that at substrate COD/N of 5, organic and nitrogen could be eliminated simultaneously, and COD removal degree, nitrification and denitrification efficiency reached 85%, 93% and 92%, respectively. With increasing substrate COD/N ratios, the specific oxygen utilization rates of nitrifying bacteria in biofilm were found to decrease, indicating that nitrifying population became less dominant. At substrate COD/N ratio of 6, excessive heterotrophs inhibited the activity of nitrifying bacteria greatly and thus led to poor nitrification process. With the help of fluorescence in situ hybridization （FISH）, Nitrosomonas and Nitrosospira were identified as dominant ammonia-oxidizing bacteria in the biofilm at substrate COD/N of 0, whereas only Nitrosospira were detected in the biofilm at COD/N ratio of 5. Nitrospira were present as dominant nitrite-oxidizing bacteria in our study. Confocal laser scanning microscopy images revealed that at substrate COD/N ratio of 0 nitrifying bacteria existed throughout the biofilm and that at COD/N ratio of 5 they were mainly distributed in the inner layer of biofilm.展开更多
N-dodecanoyl homoserine lactone（C（12）-HSL）was detected in the supernatant of an anammox granular sludge reactor（AGSR）.C（12）-HSL could enhance the specific anammox activity of anammox biomass.Adding C（12）-HSL...N-dodecanoyl homoserine lactone（C（12）-HSL）was detected in the supernatant of an anammox granular sludge reactor（AGSR）.C（12）-HSL could enhance the specific anammox activity of anammox biomass.Adding C（12）-HSL-containing AGSR supernatant into the continuously stirred tank reactors reduced the start-up time of the anammox process from80 to 66 days.Moreover,the nitrogen loading rate was also enhanced to 1.6 times that of the control reactor.AHLs could increase the secretion of extracellular polymeric substances and anammox obtained better enrichment with the addition of AHLs-containing AGSR supernatant.Denaturing gradient gel electrophoresis analysis further revealed that AHLs played a role in mediating microbial community parameters.In conclusion,adding AHL-containing supernatant could be an effective and economical way to accelerate the start-up of anammox.展开更多
A set of field experiments was conducted to investigate the effects of reed rootstocks on hydraulic properties of surface soils in the Shuangtai Estuary Wetland, Northeast China. The soil particle size distribution an...A set of field experiments was conducted to investigate the effects of reed rootstocks on hydraulic properties of surface soils in the Shuangtai Estuary Wetland, Northeast China. The soil particle size distribution and rootstock content were analyzed, and the vertical soil water profile was monitored by using a multisensory capacitance system. Hydraulic conductivity of the surface soil layer was estimated by in si-tu infiltration. The soil was silt loam with less sand; soil texture was consistent though the vertical profile, but bulk density was lower in the upper 20 era, where the fine roots were concentrated. The surface soil moisture profile changed dynamically, and variation in vertically integrated soil moisture was consistent with observed precipitation and estimated evaporation. Infiltration capacity was 30 cm＇d＂~, much larger than typical hydraulic conductivity values for silt loam with less sand. These findings suggest that fine annual roots change the soil matrix and hydraulic conductivity in surface soils. A vertical one-dimensional water transport model was presented based on Richard＇s equation. Model parameters were estimated from the soil analyses and literature data. The computation accurately reproduced the dynamic changes in moisture in surface soils containing large volumes of fine rootstock.展开更多
基金supported by the National Science Council of China (No.50578023)
文摘Two parallel carbon-membrane aerated biofilm reactors were operated at well-defined conditions to investigate the effect of substrate COD/N ratios on the performance and microbial community structure of the bioreactor. Results showed that at substrate COD/N of 5, organic and nitrogen could be eliminated simultaneously, and COD removal degree, nitrification and denitrification efficiency reached 85%, 93% and 92%, respectively. With increasing substrate COD/N ratios, the specific oxygen utilization rates of nitrifying bacteria in biofilm were found to decrease, indicating that nitrifying population became less dominant. At substrate COD/N ratio of 6, excessive heterotrophs inhibited the activity of nitrifying bacteria greatly and thus led to poor nitrification process. With the help of fluorescence in situ hybridization （FISH）, Nitrosomonas and Nitrosospira were identified as dominant ammonia-oxidizing bacteria in the biofilm at substrate COD/N of 0, whereas only Nitrosospira were detected in the biofilm at COD/N ratio of 5. Nitrospira were present as dominant nitrite-oxidizing bacteria in our study. Confocal laser scanning microscopy images revealed that at substrate COD/N ratio of 0 nitrifying bacteria existed throughout the biofilm and that at COD/N ratio of 5 they were mainly distributed in the inner layer of biofilm.
基金supported by the Major State Science and Technology Water Projects (No. 2013ZX07202010)
文摘N-dodecanoyl homoserine lactone（C（12）-HSL）was detected in the supernatant of an anammox granular sludge reactor（AGSR）.C（12）-HSL could enhance the specific anammox activity of anammox biomass.Adding C（12）-HSL-containing AGSR supernatant into the continuously stirred tank reactors reduced the start-up time of the anammox process from80 to 66 days.Moreover,the nitrogen loading rate was also enhanced to 1.6 times that of the control reactor.AHLs could increase the secretion of extracellular polymeric substances and anammox obtained better enrichment with the addition of AHLs-containing AGSR supernatant.Denaturing gradient gel electrophoresis analysis further revealed that AHLs played a role in mediating microbial community parameters.In conclusion,adding AHL-containing supernatant could be an effective and economical way to accelerate the start-up of anammox.
文摘A set of field experiments was conducted to investigate the effects of reed rootstocks on hydraulic properties of surface soils in the Shuangtai Estuary Wetland, Northeast China. The soil particle size distribution and rootstock content were analyzed, and the vertical soil water profile was monitored by using a multisensory capacitance system. Hydraulic conductivity of the surface soil layer was estimated by in si-tu infiltration. The soil was silt loam with less sand; soil texture was consistent though the vertical profile, but bulk density was lower in the upper 20 era, where the fine roots were concentrated. The surface soil moisture profile changed dynamically, and variation in vertically integrated soil moisture was consistent with observed precipitation and estimated evaporation. Infiltration capacity was 30 cm＇d＂~, much larger than typical hydraulic conductivity values for silt loam with less sand. These findings suggest that fine annual roots change the soil matrix and hydraulic conductivity in surface soils. A vertical one-dimensional water transport model was presented based on Richard＇s equation. Model parameters were estimated from the soil analyses and literature data. The computation accurately reproduced the dynamic changes in moisture in surface soils containing large volumes of fine rootstock.
