Researchers have shown significant interest in modulating the peroxidase-like activity of nanozymes.Among these,bimetallic nanozymes have shown superior peroxidase-like activity over monometallic counterparts,offering...Researchers have shown significant interest in modulating the peroxidase-like activity of nanozymes.Among these,bimetallic nanozymes have shown superior peroxidase-like activity over monometallic counterparts,offering enhanced performance and cost-efficiency in nanozyme designs.Herein,bimetallic nanozymes comprising nickel(Ni)and osmium(Os)incorporated into hyaluronate(HA)have been developed,resulting in HA-Nin/Os nanoclusters.Subsequently,comprehensive characterizations have been conducted.Further investigation has revealed that HA-Nin/Os efficiently catalyzed 3,3,5,5-tetramethylbenzidine(TMB)oxidation with hydrogen peroxide(H_(2)O_(2)),confirming its peroxidase-like behavior and role as a nanozyme.Impressively,HA-Ni_(2)/Os(Ni/Os=2:1)displays heightened substrate affinity,accelerated reaction rates,enhanced hydroxyl radical production in acidic conditions,and exhibits activity unit of 1224 U/mg,representing more than two-fold increase compared to non-Ni-supported Os nanozyme.Theoretical calculations indicate that Ni support enhances the peroxidase-like process of Os nanozyme by improving H_(2)O_(2) adsorption and TMB oxidation.Crucially,the support of Ni does not significantly alter the other enzyme-like activities of Os nanozymes,thereby enabling Ni to selectively enhance their peroxidase-like activity.In terms of application,the peroxidase-like ability of HA-Ni_(2)/Os,facilitated by HA's carboxyl groups enabling crosslinking,proves effective in a squamous carcinoma antigen immunoassay.Moreover,HA-Ni_(2)/Os exhibit reliable stability,promising as a peroxidase substitute.This work underscores the advantages of incorporating Ni into Os,specifically enhancing peroxidase-like activity,highlighting the potential of Os bimetallic nanozymes for peroxidase-based applications.展开更多
基金financial support from the Natural Science Foundation of Fujian Province(No.2022J01271)the Joint Funds for the Innovation of Science and Technology,Fujian Province(No.2023Y9226)+1 种基金the Introduced High-Level Talent Team Project of Quanzhou City(No.2023CT008)the Doctoral Research Foundation Project of the Second Affiliated Hospital of Fujian Medical University(No.BS202201)。
文摘Researchers have shown significant interest in modulating the peroxidase-like activity of nanozymes.Among these,bimetallic nanozymes have shown superior peroxidase-like activity over monometallic counterparts,offering enhanced performance and cost-efficiency in nanozyme designs.Herein,bimetallic nanozymes comprising nickel(Ni)and osmium(Os)incorporated into hyaluronate(HA)have been developed,resulting in HA-Nin/Os nanoclusters.Subsequently,comprehensive characterizations have been conducted.Further investigation has revealed that HA-Nin/Os efficiently catalyzed 3,3,5,5-tetramethylbenzidine(TMB)oxidation with hydrogen peroxide(H_(2)O_(2)),confirming its peroxidase-like behavior and role as a nanozyme.Impressively,HA-Ni_(2)/Os(Ni/Os=2:1)displays heightened substrate affinity,accelerated reaction rates,enhanced hydroxyl radical production in acidic conditions,and exhibits activity unit of 1224 U/mg,representing more than two-fold increase compared to non-Ni-supported Os nanozyme.Theoretical calculations indicate that Ni support enhances the peroxidase-like process of Os nanozyme by improving H_(2)O_(2) adsorption and TMB oxidation.Crucially,the support of Ni does not significantly alter the other enzyme-like activities of Os nanozymes,thereby enabling Ni to selectively enhance their peroxidase-like activity.In terms of application,the peroxidase-like ability of HA-Ni_(2)/Os,facilitated by HA's carboxyl groups enabling crosslinking,proves effective in a squamous carcinoma antigen immunoassay.Moreover,HA-Ni_(2)/Os exhibit reliable stability,promising as a peroxidase substitute.This work underscores the advantages of incorporating Ni into Os,specifically enhancing peroxidase-like activity,highlighting the potential of Os bimetallic nanozymes for peroxidase-based applications.
