For some important object recognition applications such as intelligent robots and unmanned driving, images are collected on a consecutive basis and associated among themselves, besides, the scenes have steady prior fe...For some important object recognition applications such as intelligent robots and unmanned driving, images are collected on a consecutive basis and associated among themselves, besides, the scenes have steady prior features. Yet existing technologies do not take full advantage of this information. In order to take object recognition further than existing algorithms in the above application, an object recognition method that fuses temporal sequence with scene priori information is proposed. This method first employs YOLOv3 as the basic algorithm to recognize objects in single-frame images, then the DeepSort algorithm to establish association among potential objects recognized in images of different moments, and finally the confidence fusion method and temporal boundary processing method designed herein to fuse, at the decision level, temporal sequence information with scene priori information. Experiments using public datasets and self-built industrial scene datasets show that due to the expansion of information sources, the quality of single-frame images has less impact on the recognition results, whereby the object recognition is greatly improved. It is presented herein as a widely applicable framework for the fusion of information under multiple classes. All the object recognition algorithms that output object class, location information and recognition confidence at the same time can be integrated into this information fusion framework to improve performance.展开更多
As a potential vectored vaccine,Newcastle disease virus(NDV)has been subject to various studies for vaccine development,while relatively little research has outlined the immunomodulatory effect of the virus in antigen...As a potential vectored vaccine,Newcastle disease virus(NDV)has been subject to various studies for vaccine development,while relatively little research has outlined the immunomodulatory effect of the virus in antigen presentation.To elucidate the key inhibitory factor in regulating the interaction of infected dendritic cells(DCs)and T cells,DCs were pretreated with the NDV vaccine strain LaSota as an inhibitor and stimulated with lipopolysaccharide(LPS)for further detection by enzyme-linked immunosorbent assay(ELISA),flow cytometry,immunoblotting,and quantitative real-time polymerase chain reaction(qRT-PCR).The results revealed that NDV infection resulted in the inhibition of interleukin(IL)-12p40 in DCs through a p38 mitogen-activated protein kinase(MAPK)-dependent manner,thus inhibiting the synthesis of IL-12p70,leading to the reduction in T cell proliferation and the secretion of interferon-(IFN-),tumor necrosis factor-α(TNF-α),and IL-6 induced by DCs.Consequently,downregulated cytokines accelerated the infection and viral transmission from DCs to T cells.Furthermore,several other strains of NDV also exhibited inhibitory activity.The current study reveals that NDV can modulate the intensity of the innate-adaptive immune cell crosstalk critically toward viral invasion improvement,highlighting a novel mechanism of virus-induced immunosuppression and providing new perspectives on the improvement of NDV-vectored vaccine.展开更多
During seed germination,the cotton chaperone protein HSP24.7 regulates the release,from the mitochondrial electron transport chain,of reactive oxygen species(ROS),a stimulative signal regulating germination.The functi...During seed germination,the cotton chaperone protein HSP24.7 regulates the release,from the mitochondrial electron transport chain,of reactive oxygen species(ROS),a stimulative signal regulating germination.The function of HSP24.7 during vegetative stages remains largely unknown.Here we propose that suppression of Gh HSP24.7 in cotton seedlings increases tolerance to heat and drought stress.Elevation of Gh HSP24.7 was found to be positively associated with endogenous levels of ROS.We identified a new client protein of Gh HSP24.7,cotton lysine deacetylase(Gh HDA14),which is involved in mitochondrial protein modification.Elevated levels of Gh HSP24.7 suppressed deacetylase activity in mitochondria,leading to increased acetylation of mitochondrial proteins enriched in the subunit of Ftype ATPase,V-type ATPase,and cytochrome C reductase,ultimately reducing leaf ATP content.Consequently,in combination with altered ROS content,Gh HSP24.7 transgenic lines were unable to coordinate stomatal closure under stress.The regulation circuit composed of Gh HSP24.7 and Gh HDA14 represents a post-translation level mechanism in plant abiotic stress responses that integrates the regulation of ROS and ATP.展开更多
The linear instabilities of incompressible confluent mixing layer and boundary layer were analyzed.The mixing layers include wake,shear layer and their combination.The mean velocity profile of confluent flow is taken ...The linear instabilities of incompressible confluent mixing layer and boundary layer were analyzed.The mixing layers include wake,shear layer and their combination.The mean velocity profile of confluent flow is taken as a superposition of a hyperbolic and exponential function to model a mixing layer and the Blasius similarity solution for a flat plate boundary layer.The stability equation of confluent flow was solved by using the global numerical method.The unstable modes associated with both the mixing and boundary layers were identified.They are the boundary layer mode,mixing layer mode 1（nearly symmetrical mode）and mode 2（nearly anti-symmetrical mode）.The interactions between the mixing layer stability and the boundary layer stability were examined.As the mixing layer approaches the boundary layer,the neutral curves of the boundary layer mode move to the upper left,the resulting critical Reynolds number decreases,and the growth rate of the most unstable mode increases.The wall tends to stabilize the mixing layer modes at low frequency.In addition,the mode switching behavior of the relative level of the spatial growth rate between the mixing layer mode 1 and mode 2 with the velocity ratio is found to occur at low frequency.展开更多
This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that th...This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed β-Ⅲ-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing β-Ⅲ-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions.展开更多
Lotus(Nelumbo)is one of the top ten flowers in China,which has high ornamental,edible and medicinal value.Lotus has a been cultivated for thousands of years.Through discovery and cultivation,more than 4,000 cultivars ...Lotus(Nelumbo)is one of the top ten flowers in China,which has high ornamental,edible and medicinal value.Lotus has a been cultivated for thousands of years.Through discovery and cultivation,more than 4,000 cultivars have been recorded.However,the information related to lotus breeding is quite scattered,and the related genetic rules and trait formation mechanisms are still poorly understood,which has caused a greater impact on lotus genetic breeding.This article systematically introduces lotus germplasm resources,including wild species and cultivated species,summarizes lotus breeding methods and breeding directions,and focuses on the latest progress in the isolation and functional identification of structural and regulatory genes related to important horticultural traits.Prospects for the protection and utilization of lotus resources,breeding and industrialization are reported.展开更多
This study focuses on the use of we-media by small-and medium-sized enterprises(SMEs)to disclose internal corporate social responsibility(ICSR)under the impact of the 2019 novel coronavirus disease(COVID-19).Study 1 i...This study focuses on the use of we-media by small-and medium-sized enterprises(SMEs)to disclose internal corporate social responsibility(ICSR)under the impact of the 2019 novel coronavirus disease(COVID-19).Study 1 interprets the catalyst effect of COVID-19 on the externalization of SMEs'ICSR.The fuzzy grading evaluation method is initially verified.Under the impact of COVID-19,SMEs fulfilling their ICSR can enhance consumer brand attitudes.Study 2 uses a structural equation model and empirical analysis of 946 effective samples and finds that consumers perceive the self-sacrifice of corporations during the coronavirus disease period.SMEs can fulfill their ICSR to enhance the internal explanation mechanism of consumer brand attitudes and the moderating role of enterprise losses.展开更多
文摘For some important object recognition applications such as intelligent robots and unmanned driving, images are collected on a consecutive basis and associated among themselves, besides, the scenes have steady prior features. Yet existing technologies do not take full advantage of this information. In order to take object recognition further than existing algorithms in the above application, an object recognition method that fuses temporal sequence with scene priori information is proposed. This method first employs YOLOv3 as the basic algorithm to recognize objects in single-frame images, then the DeepSort algorithm to establish association among potential objects recognized in images of different moments, and finally the confidence fusion method and temporal boundary processing method designed herein to fuse, at the decision level, temporal sequence information with scene priori information. Experiments using public datasets and self-built industrial scene datasets show that due to the expansion of information sources, the quality of single-frame images has less impact on the recognition results, whereby the object recognition is greatly improved. It is presented herein as a widely applicable framework for the fusion of information under multiple classes. All the object recognition algorithms that output object class, location information and recognition confidence at the same time can be integrated into this information fusion framework to improve performance.
文摘As a potential vectored vaccine,Newcastle disease virus(NDV)has been subject to various studies for vaccine development,while relatively little research has outlined the immunomodulatory effect of the virus in antigen presentation.To elucidate the key inhibitory factor in regulating the interaction of infected dendritic cells(DCs)and T cells,DCs were pretreated with the NDV vaccine strain LaSota as an inhibitor and stimulated with lipopolysaccharide(LPS)for further detection by enzyme-linked immunosorbent assay(ELISA),flow cytometry,immunoblotting,and quantitative real-time polymerase chain reaction(qRT-PCR).The results revealed that NDV infection resulted in the inhibition of interleukin(IL)-12p40 in DCs through a p38 mitogen-activated protein kinase(MAPK)-dependent manner,thus inhibiting the synthesis of IL-12p70,leading to the reduction in T cell proliferation and the secretion of interferon-(IFN-),tumor necrosis factor-α(TNF-α),and IL-6 induced by DCs.Consequently,downregulated cytokines accelerated the infection and viral transmission from DCs to T cells.Furthermore,several other strains of NDV also exhibited inhibitory activity.The current study reveals that NDV can modulate the intensity of the innate-adaptive immune cell crosstalk critically toward viral invasion improvement,highlighting a novel mechanism of virus-induced immunosuppression and providing new perspectives on the improvement of NDV-vectored vaccine.
基金financially supported by the National Natural Science Foundation of China(NSFC 31901430,31971985)Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang(2019R01002)+1 种基金Fundamental Research Funds for the Central UniversitiesJiangsu Collaborative Innovation Center for Modern Crop Production(JCIC-MCP)。
文摘During seed germination,the cotton chaperone protein HSP24.7 regulates the release,from the mitochondrial electron transport chain,of reactive oxygen species(ROS),a stimulative signal regulating germination.The function of HSP24.7 during vegetative stages remains largely unknown.Here we propose that suppression of Gh HSP24.7 in cotton seedlings increases tolerance to heat and drought stress.Elevation of Gh HSP24.7 was found to be positively associated with endogenous levels of ROS.We identified a new client protein of Gh HSP24.7,cotton lysine deacetylase(Gh HDA14),which is involved in mitochondrial protein modification.Elevated levels of Gh HSP24.7 suppressed deacetylase activity in mitochondria,leading to increased acetylation of mitochondrial proteins enriched in the subunit of Ftype ATPase,V-type ATPase,and cytochrome C reductase,ultimately reducing leaf ATP content.Consequently,in combination with altered ROS content,Gh HSP24.7 transgenic lines were unable to coordinate stomatal closure under stress.The regulation circuit composed of Gh HSP24.7 and Gh HDA14 represents a post-translation level mechanism in plant abiotic stress responses that integrates the regulation of ROS and ATP.
基金supported by the National Natural Science Foundation of China (No. 51476152)
文摘The linear instabilities of incompressible confluent mixing layer and boundary layer were analyzed.The mixing layers include wake,shear layer and their combination.The mean velocity profile of confluent flow is taken as a superposition of a hyperbolic and exponential function to model a mixing layer and the Blasius similarity solution for a flat plate boundary layer.The stability equation of confluent flow was solved by using the global numerical method.The unstable modes associated with both the mixing and boundary layers were identified.They are the boundary layer mode,mixing layer mode 1（nearly symmetrical mode）and mode 2（nearly anti-symmetrical mode）.The interactions between the mixing layer stability and the boundary layer stability were examined.As the mixing layer approaches the boundary layer,the neutral curves of the boundary layer mode move to the upper left,the resulting critical Reynolds number decreases,and the growth rate of the most unstable mode increases.The wall tends to stabilize the mixing layer modes at low frequency.In addition,the mode switching behavior of the relative level of the spatial growth rate between the mixing layer mode 1 and mode 2 with the velocity ratio is found to occur at low frequency.
基金funded by the National High-Tech Research and Development Program of China(863Program),No.2008AA101005
文摘This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed β-Ⅲ-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing β-Ⅲ-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions.
基金supported by the National Natural Science Foundation of China(Grants No's 31971710 and 32071829)Fundamental Research Funds of the Central Government(Special Project of Lotus Germplasm Resources)(KYZZ2021003).
文摘Lotus(Nelumbo)is one of the top ten flowers in China,which has high ornamental,edible and medicinal value.Lotus has a been cultivated for thousands of years.Through discovery and cultivation,more than 4,000 cultivars have been recorded.However,the information related to lotus breeding is quite scattered,and the related genetic rules and trait formation mechanisms are still poorly understood,which has caused a greater impact on lotus genetic breeding.This article systematically introduces lotus germplasm resources,including wild species and cultivated species,summarizes lotus breeding methods and breeding directions,and focuses on the latest progress in the isolation and functional identification of structural and regulatory genes related to important horticultural traits.Prospects for the protection and utilization of lotus resources,breeding and industrialization are reported.
基金supported by the Fundamental Research Funds for the Central Universities,and the Research Funds of Renmin University of China(20XNL01B).
文摘This study focuses on the use of we-media by small-and medium-sized enterprises(SMEs)to disclose internal corporate social responsibility(ICSR)under the impact of the 2019 novel coronavirus disease(COVID-19).Study 1 interprets the catalyst effect of COVID-19 on the externalization of SMEs'ICSR.The fuzzy grading evaluation method is initially verified.Under the impact of COVID-19,SMEs fulfilling their ICSR can enhance consumer brand attitudes.Study 2 uses a structural equation model and empirical analysis of 946 effective samples and finds that consumers perceive the self-sacrifice of corporations during the coronavirus disease period.SMEs can fulfill their ICSR to enhance the internal explanation mechanism of consumer brand attitudes and the moderating role of enterprise losses.
