As a natural genetic reservoir, wild rice contains many favorable alleles and mutations conferring high yield and resistance to biotic and abiotic stresses. However, there are few reports describing favorable genes or...As a natural genetic reservoir, wild rice contains many favorable alleles and mutations conferring high yield and resistance to biotic and abiotic stresses. However, there are few reports describing favorable genes or QTL from the AA genome wild rice O. longistaminata, which is characterized by tall and robust habit and long tassels and anthers and shows high potential for use in cultivated rice improvement. We constructed a stable BC_(2)F_(20) backcross inbred line(BIL) population of 152 lines from the cross of 9311 × O.longistaminat. Some BILs showed large panicles, large seeds, and strong resistance to rice false smut, bacterial leaf blight, rice blast spot, and brown planthopper. Genomic resequencing showed that the 152 BILs covered about 99.6% of the O. longistaminata genome. QTL mapping with 2432 bin markers revealed 13QTL associated with seven yield traits and eight with resistance to brown planthopper and to four diseases. Of these QTL, 12 for grain yield and 11 for pest and disease resistance are novel in Oryza species.A large-panicle NIL1880 line containing QTL qPB8.1 showed a nearly 50% increase in spikelet number and27.5% in grain yield compared to the recurrent parent 9311. These findings support the potential value of O. longistaminata for cultivated rice improvement.展开更多
Histone methylation is involved in a wide range of biological regulation in plants,and is conducted by three major components,including methyltransferases,demethylases,and histone readers.Compared with the other two c...Histone methylation is involved in a wide range of biological regulation in plants,and is conducted by three major components,including methyltransferases,demethylases,and histone readers.Compared with the other two components,research on histone readers is relatively limited.In this study,we demonstrate that OsSHH5 functions as an H3K9me1 reader to regulate rice disease resistance,tillering,and grain yield.Loss of OsSHH5 function significantly enhances both grain yield and disease resistance.Mechanistically,OsSHH5 recruits the H3K9 methyltransferase SGD733 and binds to H3K9me1,thereby maintaining H3K9me1 enrichment and facilitating gene silencing.In leaves,OsSHH5 interacts with the transcriptional factor HPY1 to target the resistance-related genes OsWAKg52 and OsWRKY81,maintaining their H3K9me1 levels and suppressing multiple PAMP-triggered immune responses,which ultimately reduces rice disease resistance.In tiller buds,OsSHH5 interacts with the transcriptional factor TCP19 to target the tillering-related gene OsNGR5,maintaining its H3K9me1 enrichment and inhibition of tillering,leading to reduced yield.Collectively,these findings reveal that OsSHH5 plays a vital role in integrating immune response,tillering,and grain yield in rice,providing new insights into the function of histone readers and offering a new strategy to improve rice yield and disease resistance.展开更多
1 Introduction Entity resolution （ER）, also referred to as record linkage and entity matching [1], is a long-standing challenge, existing in various data management systems, particularly data integration and cleanin...1 Introduction Entity resolution （ER）, also referred to as record linkage and entity matching [1], is a long-standing challenge, existing in various data management systems, particularly data integration and cleaning systems. As so often happens, there exist multiple data sources which store duplicate real-world en- tity information in different descriptions, incurred by mis- spellings, typos, diverse name conventions, random usage of the abbreviation or full name, ongoing changes such as in DBpedia, and so forth. The purpose of ER is to determine whether two data records describe the same real-world entity.展开更多
Grain number per panicle,a crucial component for rice grain yield,is usually determined by the primary and secondary branches of spikelets.Normally,the tips of the primary and secondary branches of spikelets are occup...Grain number per panicle,a crucial component for rice grain yield,is usually determined by the primary and secondary branches of spikelets.Normally,the tips of the primary and secondary branches of spikelets are occupied by a single grain.Occasionally,this phenotype can be disrupted by two or three complete spikelets or grains clustered on the branch tips,significantly altering the spatial arrangement of spikelet and the structure of the panicle,thus impacting grain yield (Ren et al.,2017,2020;Zhang et al.,2017).展开更多
基金partly granted from the National Natural Science Foundation of China(U20A2023, 31870322)the Creative Research Groups of the Natural Science Foundation of Hubei Province,China (2020CFA009)the Hubei Hongshan Laboratory (2021hszd010)。
文摘As a natural genetic reservoir, wild rice contains many favorable alleles and mutations conferring high yield and resistance to biotic and abiotic stresses. However, there are few reports describing favorable genes or QTL from the AA genome wild rice O. longistaminata, which is characterized by tall and robust habit and long tassels and anthers and shows high potential for use in cultivated rice improvement. We constructed a stable BC_(2)F_(20) backcross inbred line(BIL) population of 152 lines from the cross of 9311 × O.longistaminat. Some BILs showed large panicles, large seeds, and strong resistance to rice false smut, bacterial leaf blight, rice blast spot, and brown planthopper. Genomic resequencing showed that the 152 BILs covered about 99.6% of the O. longistaminata genome. QTL mapping with 2432 bin markers revealed 13QTL associated with seven yield traits and eight with resistance to brown planthopper and to four diseases. Of these QTL, 12 for grain yield and 11 for pest and disease resistance are novel in Oryza species.A large-panicle NIL1880 line containing QTL qPB8.1 showed a nearly 50% increase in spikelet number and27.5% in grain yield compared to the recurrent parent 9311. These findings support the potential value of O. longistaminata for cultivated rice improvement.
基金supported by the National Natural Science Foundation of China(32470391,32401801,and 31870322)the Open Competitive Project of the Wuhan East Lake high-tech Zone(2023KJB220)the Key Research and Development Program of Hubei Province(2025BBA002).
文摘Histone methylation is involved in a wide range of biological regulation in plants,and is conducted by three major components,including methyltransferases,demethylases,and histone readers.Compared with the other two components,research on histone readers is relatively limited.In this study,we demonstrate that OsSHH5 functions as an H3K9me1 reader to regulate rice disease resistance,tillering,and grain yield.Loss of OsSHH5 function significantly enhances both grain yield and disease resistance.Mechanistically,OsSHH5 recruits the H3K9 methyltransferase SGD733 and binds to H3K9me1,thereby maintaining H3K9me1 enrichment and facilitating gene silencing.In leaves,OsSHH5 interacts with the transcriptional factor HPY1 to target the resistance-related genes OsWAKg52 and OsWRKY81,maintaining their H3K9me1 levels and suppressing multiple PAMP-triggered immune responses,which ultimately reduces rice disease resistance.In tiller buds,OsSHH5 interacts with the transcriptional factor TCP19 to target the tillering-related gene OsNGR5,maintaining its H3K9me1 enrichment and inhibition of tillering,leading to reduced yield.Collectively,these findings reveal that OsSHH5 plays a vital role in integrating immune response,tillering,and grain yield in rice,providing new insights into the function of histone readers and offering a new strategy to improve rice yield and disease resistance.
基金We thank Murtadha Ahmed, Yiyi Li, Ping Zhong, YanyanWang, and Jing Su for their invaluable suggestions. This work was supported by the Ministry of Science and Technology of China, National Key Research and Development Program （2016YFB1000703）, and the National Natural Science Foundation of China （Grant Nos. 61732014, 61332006, 61472321, 61502390, and 61672432）.
文摘1 Introduction Entity resolution （ER）, also referred to as record linkage and entity matching [1], is a long-standing challenge, existing in various data management systems, particularly data integration and cleaning systems. As so often happens, there exist multiple data sources which store duplicate real-world en- tity information in different descriptions, incurred by mis- spellings, typos, diverse name conventions, random usage of the abbreviation or full name, ongoing changes such as in DBpedia, and so forth. The purpose of ER is to determine whether two data records describe the same real-world entity.
基金supported by the National Natural Science Foundation of China(U20A2023)the Open Competitive Project of the Donghu New&High Technology Development Zone,Wuhan,China(2023KJB220)。
文摘Grain number per panicle,a crucial component for rice grain yield,is usually determined by the primary and secondary branches of spikelets.Normally,the tips of the primary and secondary branches of spikelets are occupied by a single grain.Occasionally,this phenotype can be disrupted by two or three complete spikelets or grains clustered on the branch tips,significantly altering the spatial arrangement of spikelet and the structure of the panicle,thus impacting grain yield (Ren et al.,2017,2020;Zhang et al.,2017).
