A microbial strain designated Bacillus licheniformis QX928 was screened from hot springs in Sichuan Province,China,and a compound generated in the culture of this strain clearly inhibited Pseudomonas aeruginosa ATCC27...A microbial strain designated Bacillus licheniformis QX928 was screened from hot springs in Sichuan Province,China,and a compound generated in the culture of this strain clearly inhibited Pseudomonas aeruginosa ATCC27853.The measured minimum inhibitory and lowest bactericidal concentrations were(13±0.17)and(22±0.72)mg L^(-1),respectively.The compound was identified as 3-isopropylhexahydro-4H-pyrido[1,2-α]pyrazine-1,4(6H)-dione(IPHPPD).A Sci Finder search revealed that IPHPPD could be the first compound synthesized by microorganisms that had both antibacterial and anti-quorum sensing properties.At low concentrations,IPHPPD interfered with the signaling factors and population effects of P.aeruginosa,thereby altering the biofilm morphology and structure.IPHPPD more strongly inhibited P.aeruginosa at high concentrations,primarily by reducing its virulence factors,cell membrane permeability and energy metabolism.A transcriptome analysis highlighted the role of IPHPPD in the transcriptional regulation of cellular metabolism and quorum sensing.Thus,the results of this study provide critical evidence that IPHPPD is a potential target for drug development to prevent and treat diseases in animals.展开更多
基金supported by the Guangdong Basic and Applied Basic Research Foundation,China(2024A1515012511 and 2022A1515012380)the Guangdong Special Project on Key Fields of Colleges and Universities,China(Rural Revitalization,2020ZDZX1020)+1 种基金the Science and Technology Special Fund Project of Maoming Science and Technology Bureau,China(2021KJZXZJGSPDX003)the Projects of Talents Rec r uitment of G uangdong Univer sit y of Petrochemical Technology,China(519033)。
文摘A microbial strain designated Bacillus licheniformis QX928 was screened from hot springs in Sichuan Province,China,and a compound generated in the culture of this strain clearly inhibited Pseudomonas aeruginosa ATCC27853.The measured minimum inhibitory and lowest bactericidal concentrations were(13±0.17)and(22±0.72)mg L^(-1),respectively.The compound was identified as 3-isopropylhexahydro-4H-pyrido[1,2-α]pyrazine-1,4(6H)-dione(IPHPPD).A Sci Finder search revealed that IPHPPD could be the first compound synthesized by microorganisms that had both antibacterial and anti-quorum sensing properties.At low concentrations,IPHPPD interfered with the signaling factors and population effects of P.aeruginosa,thereby altering the biofilm morphology and structure.IPHPPD more strongly inhibited P.aeruginosa at high concentrations,primarily by reducing its virulence factors,cell membrane permeability and energy metabolism.A transcriptome analysis highlighted the role of IPHPPD in the transcriptional regulation of cellular metabolism and quorum sensing.Thus,the results of this study provide critical evidence that IPHPPD is a potential target for drug development to prevent and treat diseases in animals.
