The use of markers and linkage map construction are important for QTL mapping in pigs.In this article,the genetic characteristics were studied and the linkage map was constructed in a pig resource population including...The use of markers and linkage map construction are important for QTL mapping in pigs.In this article,the genetic characteristics were studied and the linkage map was constructed in a pig resource population including 214 individuals by typing 39 microsatellite marker loci on Sus scrofa chromosomes,SSC4,SSC6,SSC7,SSC8,and SSC13.Results indicated that the average allele number,the average observed beterozygosity(Ho),and the average polymorphism information content(PIC)in F1 and F2 population were 3.2,0.528,0.463 and 3.2,0.496,0.447,respectively.In the pig resource population,the average informative meiosis(IM)was 217.4(44-316),and the average linkage map length between the two sexes on the five chromosomes were 172.3 cM(SSC4),168.7 cM(SSC6),191.7 cM(SSC7),197.3 cM(SSC8),and 178.3 cM(SSC13).The orders of microsatellite marker loci in the linkage maps were identical to,but the length was greater than,those of USDA-MARC reference map.The results of this research showed the genetic relationship and genetic characteristics of the microsatellite markers in the pig resource family population,and the linkage map could be used to for QTL mapping in the subsequent study.展开更多
目的:评价前列腺癌冷冻消融联合抗细胞毒性T淋巴细胞抗原-4(cytotoxic T lymphocyte antigen-4,CTLA-4)抗体对小鼠肿瘤引流淋巴结T细胞抗肿瘤免疫反应的影响。方法:建立前列腺癌小鼠模型并随机分为空白对照(A)组、冷冻消融治疗(B)组、...目的:评价前列腺癌冷冻消融联合抗细胞毒性T淋巴细胞抗原-4(cytotoxic T lymphocyte antigen-4,CTLA-4)抗体对小鼠肿瘤引流淋巴结T细胞抗肿瘤免疫反应的影响。方法:建立前列腺癌小鼠模型并随机分为空白对照(A)组、冷冻消融治疗(B)组、冷冻消融联合抗CTLA-4抗体治疗(C)组、抗CTLA-4抗体治疗(D)组。各组分别于治疗前及治疗后7、14、21天四个时间点取5只小鼠获取肿瘤引流淋巴结标本,测量各组不同时间点肿瘤大小变化,流式细胞术检测调节性T细胞(regulator T cell,Treg)和细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)比例变化,乳酸脱氢酶(LDH)法检测CTL对肿瘤细胞的杀伤活性,记录各组小鼠总生存期。结果:治疗后14天与A组的Treg比例相比,B组(9.78%±1.88%vs.6.02%±0.44%,)、C组(9.78%±1.88%vs.6.03%±0.45%)均下调(均P<0.05);与A组的CTL比例相比,B组(27.34%±2.13%vs.34.23%±1.15%)、C组(27.34%±2.13%vs.52.21%±2.53%)、D组(27.34%±2.13%vs.33.99%±1.21%)均上调(P<0.05、P<0.001、P<0.05);与A组的CTL杀伤活性相比,B组(13.32%±3.39%vs.26.36%±2.95%)、C组(13.32%±3.39%vs.45.25%±3.27%)、D组(13.32%±3.39%vs.25.31%±3.24%)均升高(P<0.05、P<0.001、P<0.05)。同时小鼠生存分析显示,B组的生存时间(34.20±6.98)天和C组的(43.60±2.88)天较A组(25.60±1.52)天明显延长(P<0.05和P<0.001),C组的生存时间(43.60±2.88)天较B组的(34.20±6.98)天明显延长(P<0.05)。结论:冷冻消融联合抗CTLA-4抗体可降低肿瘤引流淋巴结Treg比例,提高CTL比例及其杀伤活性,明显延长小鼠生存期,其具体机制尚需进一步研究。展开更多
基金This work was supported by the National 973 Projects of China(No.2006CB102102)the National Natural Science Foundation of China(No.30500358).
文摘The use of markers and linkage map construction are important for QTL mapping in pigs.In this article,the genetic characteristics were studied and the linkage map was constructed in a pig resource population including 214 individuals by typing 39 microsatellite marker loci on Sus scrofa chromosomes,SSC4,SSC6,SSC7,SSC8,and SSC13.Results indicated that the average allele number,the average observed beterozygosity(Ho),and the average polymorphism information content(PIC)in F1 and F2 population were 3.2,0.528,0.463 and 3.2,0.496,0.447,respectively.In the pig resource population,the average informative meiosis(IM)was 217.4(44-316),and the average linkage map length between the two sexes on the five chromosomes were 172.3 cM(SSC4),168.7 cM(SSC6),191.7 cM(SSC7),197.3 cM(SSC8),and 178.3 cM(SSC13).The orders of microsatellite marker loci in the linkage maps were identical to,but the length was greater than,those of USDA-MARC reference map.The results of this research showed the genetic relationship and genetic characteristics of the microsatellite markers in the pig resource family population,and the linkage map could be used to for QTL mapping in the subsequent study.
文摘目的:评价前列腺癌冷冻消融联合抗细胞毒性T淋巴细胞抗原-4(cytotoxic T lymphocyte antigen-4,CTLA-4)抗体对小鼠肿瘤引流淋巴结T细胞抗肿瘤免疫反应的影响。方法:建立前列腺癌小鼠模型并随机分为空白对照(A)组、冷冻消融治疗(B)组、冷冻消融联合抗CTLA-4抗体治疗(C)组、抗CTLA-4抗体治疗(D)组。各组分别于治疗前及治疗后7、14、21天四个时间点取5只小鼠获取肿瘤引流淋巴结标本,测量各组不同时间点肿瘤大小变化,流式细胞术检测调节性T细胞(regulator T cell,Treg)和细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)比例变化,乳酸脱氢酶(LDH)法检测CTL对肿瘤细胞的杀伤活性,记录各组小鼠总生存期。结果:治疗后14天与A组的Treg比例相比,B组(9.78%±1.88%vs.6.02%±0.44%,)、C组(9.78%±1.88%vs.6.03%±0.45%)均下调(均P<0.05);与A组的CTL比例相比,B组(27.34%±2.13%vs.34.23%±1.15%)、C组(27.34%±2.13%vs.52.21%±2.53%)、D组(27.34%±2.13%vs.33.99%±1.21%)均上调(P<0.05、P<0.001、P<0.05);与A组的CTL杀伤活性相比,B组(13.32%±3.39%vs.26.36%±2.95%)、C组(13.32%±3.39%vs.45.25%±3.27%)、D组(13.32%±3.39%vs.25.31%±3.24%)均升高(P<0.05、P<0.001、P<0.05)。同时小鼠生存分析显示,B组的生存时间(34.20±6.98)天和C组的(43.60±2.88)天较A组(25.60±1.52)天明显延长(P<0.05和P<0.001),C组的生存时间(43.60±2.88)天较B组的(34.20±6.98)天明显延长(P<0.05)。结论:冷冻消融联合抗CTLA-4抗体可降低肿瘤引流淋巴结Treg比例,提高CTL比例及其杀伤活性,明显延长小鼠生存期,其具体机制尚需进一步研究。
