AIM: To study interactions between hepatitis B virus (HBV) and interferon-a in liver- derived cells. METHODS. mRNAs were separately isolated from an HBV-transfected cell line (HepG2 2.2.15) and its parental cell line ...AIM: To study interactions between hepatitis B virus (HBV) and interferon-a in liver- derived cells. METHODS. mRNAs were separately isolated from an HBV-transfected cell line (HepG2 2.2.15) and its parental cell line (HePG2) pre- and post-interferon-a (IFN-a) treatment at 6, 24 and 48 h, followed by hybridization with a cDNA microarray filter dotted with 14 000 human genes. After hybridization and scanning of the arrays, the data were analyzed using ArrayGauge software. The microarray data were further verified by Northern blot analysis. RESULTS: Compared to HepG2 cells, 14 genes with known functions were down-regulated 3 to 12- magnitudes, while 7 genes were up-regulated 3-13 magnitudes in HepG2 2.2.15 cells prior to IFN-a treatment. After interferon-a treatment, the expression of four genes (vascular endothelial growth factor, tyrosine phosphate 1E, serine protein with IGF-binding motif and one gene of clathrin light chain) in HepG2 2.2.15 were up-regulated, while one gene encoding a GTP-binding protein, two genes of interferon-induced kinases and two proto-oncogenes were further down- regulated. Interestingly, under IFN-a treatment, a number of differentially regulated genes were new ESTs or genes with unknown functions. CONCLUSION: The up-regulated genes in HepG2 2.2.15 cell line suggested that under IFN-a treatment, these repressed cellular genes in HBV infected hepatooltes could be partially restored, while the down- regulated genes were most likely the cellular genes which could not be restored under interferon treatment. These down-regulated genes identified by microarray analysis could serve as new targets for anti-HBV drug development or for novel therapies.展开更多
近几年遥感技术发展迅速,土地利用变化监测的技术也越来越多,并且为政府合理制定土地规划提供了理论依据和技术支持,有助于国家的经济建设以及土地管理的规范。本文以河北省衡水市为研究对象,从地理空间数据云上所获得的2010年、2015年...近几年遥感技术发展迅速,土地利用变化监测的技术也越来越多,并且为政府合理制定土地规划提供了理论依据和技术支持,有助于国家的经济建设以及土地管理的规范。本文以河北省衡水市为研究对象,从地理空间数据云上所获得的2010年、2015年、2020年的衡水市Landsat影像作为基础影像数据,以ArcGIS10.2和ENVI5.3软件为平台对数据进行处理和分析。基于遥感影像的时间、光谱、空间等各种动态特征信息的变化,获得了衡水市土地变化的类型,位置和数量等相关信息。研究发现:2010—2015年,林地面积明显减少,而建设用地和农用地有所增加,这表明土地复垦力度、城市扩张趋势明显加大;2010—2020年,水体面积增加为13 km 2;耕地总量在不断增加,但增加趋势减弱,而林地在持续减弱,建设用地在不断增加,这表明城市在不断发展,呈现比较积极的趋势。最后通过分析土地类型变化和驱动力分析,旨在为衡水市扩展规划、京津冀协同发展提供数据支撑和决策依据。展开更多
Ocean is home town of wind and rain, treasury of resources. To exploit and use ocean will make great contribution to people’s existence and development. China is a great oceanic country with pre cinct sea area of 3,0...Ocean is home town of wind and rain, treasury of resources. To exploit and use ocean will make great contribution to people’s existence and development. China is a great oceanic country with pre cinct sea area of 3,000,000 km2. The eastern coastal region with only 60 km width, 15 % area and 40% of population of China, has created about 60% of GDP and has become the most developed region. But marine disaster in China is frequent and serious. From statistical data, economy loss made by surge, billow, sea ice, tsunami, red tide, oil spill, coast erosion, bay deposit, sea water intrusion, sea level rise, land salinization, sea water pollution, etc. increase quickly. Economy loss per year is one billion in the l980s, 10 billion in the l990s, exceeded 20 billion in 1996, and 30 billion in 1997. About 80% of it happened in the coastal area. So, marine disaster at present has become obstacle of society progress and economy development in coastal area. To mitigate marine disaster has become need for the economy development, also important task of the integrated coastal zone management. In this article, we briefly introduce countermeasures for marine disaster prevention, coastal zone management, and achievements made in the past 50 years, its problems now confronted, and the main countermeasures in the 21St century.展开更多
基金Supported by the Chinese State Basic Science Foundation,No.1999054105 and Med-X Foundation of Fudan University
文摘AIM: To study interactions between hepatitis B virus (HBV) and interferon-a in liver- derived cells. METHODS. mRNAs were separately isolated from an HBV-transfected cell line (HepG2 2.2.15) and its parental cell line (HePG2) pre- and post-interferon-a (IFN-a) treatment at 6, 24 and 48 h, followed by hybridization with a cDNA microarray filter dotted with 14 000 human genes. After hybridization and scanning of the arrays, the data were analyzed using ArrayGauge software. The microarray data were further verified by Northern blot analysis. RESULTS: Compared to HepG2 cells, 14 genes with known functions were down-regulated 3 to 12- magnitudes, while 7 genes were up-regulated 3-13 magnitudes in HepG2 2.2.15 cells prior to IFN-a treatment. After interferon-a treatment, the expression of four genes (vascular endothelial growth factor, tyrosine phosphate 1E, serine protein with IGF-binding motif and one gene of clathrin light chain) in HepG2 2.2.15 were up-regulated, while one gene encoding a GTP-binding protein, two genes of interferon-induced kinases and two proto-oncogenes were further down- regulated. Interestingly, under IFN-a treatment, a number of differentially regulated genes were new ESTs or genes with unknown functions. CONCLUSION: The up-regulated genes in HepG2 2.2.15 cell line suggested that under IFN-a treatment, these repressed cellular genes in HBV infected hepatooltes could be partially restored, while the down- regulated genes were most likely the cellular genes which could not be restored under interferon treatment. These down-regulated genes identified by microarray analysis could serve as new targets for anti-HBV drug development or for novel therapies.
文摘近几年遥感技术发展迅速,土地利用变化监测的技术也越来越多,并且为政府合理制定土地规划提供了理论依据和技术支持,有助于国家的经济建设以及土地管理的规范。本文以河北省衡水市为研究对象,从地理空间数据云上所获得的2010年、2015年、2020年的衡水市Landsat影像作为基础影像数据,以ArcGIS10.2和ENVI5.3软件为平台对数据进行处理和分析。基于遥感影像的时间、光谱、空间等各种动态特征信息的变化,获得了衡水市土地变化的类型,位置和数量等相关信息。研究发现:2010—2015年,林地面积明显减少,而建设用地和农用地有所增加,这表明土地复垦力度、城市扩张趋势明显加大;2010—2020年,水体面积增加为13 km 2;耕地总量在不断增加,但增加趋势减弱,而林地在持续减弱,建设用地在不断增加,这表明城市在不断发展,呈现比较积极的趋势。最后通过分析土地类型变化和驱动力分析,旨在为衡水市扩展规划、京津冀协同发展提供数据支撑和决策依据。
文摘Ocean is home town of wind and rain, treasury of resources. To exploit and use ocean will make great contribution to people’s existence and development. China is a great oceanic country with pre cinct sea area of 3,000,000 km2. The eastern coastal region with only 60 km width, 15 % area and 40% of population of China, has created about 60% of GDP and has become the most developed region. But marine disaster in China is frequent and serious. From statistical data, economy loss made by surge, billow, sea ice, tsunami, red tide, oil spill, coast erosion, bay deposit, sea water intrusion, sea level rise, land salinization, sea water pollution, etc. increase quickly. Economy loss per year is one billion in the l980s, 10 billion in the l990s, exceeded 20 billion in 1996, and 30 billion in 1997. About 80% of it happened in the coastal area. So, marine disaster at present has become obstacle of society progress and economy development in coastal area. To mitigate marine disaster has become need for the economy development, also important task of the integrated coastal zone management. In this article, we briefly introduce countermeasures for marine disaster prevention, coastal zone management, and achievements made in the past 50 years, its problems now confronted, and the main countermeasures in the 21St century.
