AIM:The prevention of recurrence of colon cancer (CC) after operation is very important for improvement of the prognosis of CC patients,especially those with micro- metastasis.The generation of fused cells between den...AIM:The prevention of recurrence of colon cancer (CC) after operation is very important for improvement of the prognosis of CC patients,especially those with micro- metastasis.The generation of fused cells between dendritic cells (DCs) and tumor cells maybe an effective approach for tumor antigen presentation in immunotherapy.In this study, we fused human colon caner SW480 cells and human peripheral blood-derived DCs to induce an antitumor activity against human CC. METHODS:CC SW480 cells and human peripheral blood- derived DCs were fused with 500 mL/L polyethylene glycol (PEG). RESULTS:The specific T cell responses activated by fusion cells (FCs),were observed.About 100 mL/L to 160 mL/L of the PEG-treated non-adherent cells with fluorescences were considered to be dendritomas that highly expressed the key molecules for antigen presentation in our five cases.In vitro studies showed that fusions effectively activated CD8^+ T lymphocytes to secrete interferon-γ.The early apoptotic ratio of the colon cancer SW480 cells was higher than that of controls,which was affected by cytotoxic T lymphocytes (CTLs) stimulated by dendritomas. CONCLUSION:The data indicate that fusion of tumor cells with DCs is an attractive strategy to induce tumor rejection.展开更多
We report a case of undifferentiated (embryonal)sarcoma of the liver (UESL), which showed cystic formation in a 20-year-old man with no prior history of any hepatitis or liver cirrhosis. He was admitted with abdominal...We report a case of undifferentiated (embryonal)sarcoma of the liver (UESL), which showed cystic formation in a 20-year-old man with no prior history of any hepatitis or liver cirrhosis. He was admitted with abdominal pain and a palpable epigastric mass. The physical examination findings were unremarkable except for a tenderness mass and the results of routine laboratory studies were all within normal limits. Abdominal ultrasound and computed tomography (CT) both showed a cystic mass in the left hepatic lobe. Subsequently, the patient underwent a tumor excision and another two times of hepatectomy because of tumor recurrence.Immunohistochemical study results showed that the tumor cells were positive for vimentin, alpha-1-antichymotrypsin (AACT) and desmin staining, and negative for alphafetoprotein (AFP), and eosinophilic hyaline globules in the cytoplasm of some giant cells were strongly positive for periodic acid-Schiff (PAS) staining. The pathological diagnosis was UESL. The patient is still alive with no tumor recurrence for four months.展开更多
OBJECTIVE To investigate the effect of microRNA-32 on cold-induced thermogenesis and brown adipocyte energy metabolism.METHODS To apply the cold-induced thermogenesis model in mice,8-10 week old male C57Bl6 mice were ...OBJECTIVE To investigate the effect of microRNA-32 on cold-induced thermogenesis and brown adipocyte energy metabolism.METHODS To apply the cold-induced thermogenesis model in mice,8-10 week old male C57Bl6 mice were placed within a 6℃fridge for 7d.Control microRNA inhibitor or miR-32 inhibitor(10mg·kg-1)was administered via intraperitoneal injection 16 hbefore the mice were placed in the fridge.Daily core body temperatures were taken using a rectal temperature probe.Mice were euthanized after 7dand brown adipose tissue(BAT),inguinal and epididymal white adipose tissue(WAT),skeletal muscle and liver tissue analysed for changes in morphology and gene expression.RESULTS miR-32 inhibition in vivoinhibits the emergence of beige cells,which function like BAT cells,within WAT.In silico prediction and gene ontology analysis identified Tob1 as a likely target gene of miR-32.miR-32 inhibition led to increased expression of Tob1 whilst mutation of target sequence abolished this effect.Expression of brown adipose markers such as Ucp1,Pgc1α,Pparαand Prdm16 were significantly reduced in inguinal white adipose tissue(P<0.05).There was also a significant decrease in serumfgf21 levels due to the inhibition of Fgf21 expression in BAT(P<0.05).p38/MAPK signalling in brown adipose tissue was also significantly inhibited within brown adipose tissue leading to decreased fgf21 expression and secretion.CONCLUSION Our study shows that miR-32 plays a crucial role in stimulating beige cell emergence by activating p38/MAPK signalling during cold thermogenesis.miR-32 may prove effective as a treatment for obesity by activating cold-induced thermogenesis leading to increased energy metabolism.展开更多
Various lignocellulosic materials such as wood, agricultural and forest residues has the potential to be valuable substitute for, or complement to, commercial sorbents for removing heavy metal ions or dyes from waste ...Various lignocellulosic materials such as wood, agricultural and forest residues has the potential to be valuable substitute for, or complement to, commercial sorbents for removing heavy metal ions or dyes from waste water or spilled oil from inland water or sea. More than 9 million tons of straw pulp are produced annually in china, which account for about 90% of the world’s total straw pulp. However, huge quantity of remain straw is not used as industrial raw material and is burnt in the fields or on the side of road. These resources can be chemical modified such as acetylation. Modified straws have the characteristics of low cost, high capacity, quick uptake, and easy to desorb. This paper reviews the current status of the technology for modified agricultural residues, which focus on hemicellulose and cellulose. The potential of these natural sorbents in main industry is also indicated.展开更多
Both dependent and independent model algorithms are designed with genetic algorithm (GA) to retrieve aerosol size data from multispectral extinction measurements. Compared with the traditional dependent model algorith...Both dependent and independent model algorithms are designed with genetic algorithm (GA) to retrieve aerosol size data from multispectral extinction measurements. Compared with the traditional dependent model algorithm, e.g., simplex, GA can locate the global optimized solution instead of local ones. As an independent model algorithm, when combined with B-splines, GA gives consistent results with Chahine and Phillip-Twomey-NNLS algorithms. Numerical simulations also show that GA has high stability and good resistance to relatively higher error levels. For a population size of 50 in the present paper, the feasible ranges for genetic operators pc and pm are found to be [0.01, 0.5] and [0.01, 0.15], respectively, and the generation number Gen_Max should be larger than 250.展开更多
基金Supported by Technology Foundation of Ministry of Education,China
文摘AIM:The prevention of recurrence of colon cancer (CC) after operation is very important for improvement of the prognosis of CC patients,especially those with micro- metastasis.The generation of fused cells between dendritic cells (DCs) and tumor cells maybe an effective approach for tumor antigen presentation in immunotherapy.In this study, we fused human colon caner SW480 cells and human peripheral blood-derived DCs to induce an antitumor activity against human CC. METHODS:CC SW480 cells and human peripheral blood- derived DCs were fused with 500 mL/L polyethylene glycol (PEG). RESULTS:The specific T cell responses activated by fusion cells (FCs),were observed.About 100 mL/L to 160 mL/L of the PEG-treated non-adherent cells with fluorescences were considered to be dendritomas that highly expressed the key molecules for antigen presentation in our five cases.In vitro studies showed that fusions effectively activated CD8^+ T lymphocytes to secrete interferon-γ.The early apoptotic ratio of the colon cancer SW480 cells was higher than that of controls,which was affected by cytotoxic T lymphocytes (CTLs) stimulated by dendritomas. CONCLUSION:The data indicate that fusion of tumor cells with DCs is an attractive strategy to induce tumor rejection.
文摘We report a case of undifferentiated (embryonal)sarcoma of the liver (UESL), which showed cystic formation in a 20-year-old man with no prior history of any hepatitis or liver cirrhosis. He was admitted with abdominal pain and a palpable epigastric mass. The physical examination findings were unremarkable except for a tenderness mass and the results of routine laboratory studies were all within normal limits. Abdominal ultrasound and computed tomography (CT) both showed a cystic mass in the left hepatic lobe. Subsequently, the patient underwent a tumor excision and another two times of hepatectomy because of tumor recurrence.Immunohistochemical study results showed that the tumor cells were positive for vimentin, alpha-1-antichymotrypsin (AACT) and desmin staining, and negative for alphafetoprotein (AFP), and eosinophilic hyaline globules in the cytoplasm of some giant cells were strongly positive for periodic acid-Schiff (PAS) staining. The pathological diagnosis was UESL. The patient is still alive with no tumor recurrence for four months.
基金The project supported by Singapore Institute for Clinical Sciences(SICS)A*STAR Singapore and BMRC Young Investigator Grant
文摘OBJECTIVE To investigate the effect of microRNA-32 on cold-induced thermogenesis and brown adipocyte energy metabolism.METHODS To apply the cold-induced thermogenesis model in mice,8-10 week old male C57Bl6 mice were placed within a 6℃fridge for 7d.Control microRNA inhibitor or miR-32 inhibitor(10mg·kg-1)was administered via intraperitoneal injection 16 hbefore the mice were placed in the fridge.Daily core body temperatures were taken using a rectal temperature probe.Mice were euthanized after 7dand brown adipose tissue(BAT),inguinal and epididymal white adipose tissue(WAT),skeletal muscle and liver tissue analysed for changes in morphology and gene expression.RESULTS miR-32 inhibition in vivoinhibits the emergence of beige cells,which function like BAT cells,within WAT.In silico prediction and gene ontology analysis identified Tob1 as a likely target gene of miR-32.miR-32 inhibition led to increased expression of Tob1 whilst mutation of target sequence abolished this effect.Expression of brown adipose markers such as Ucp1,Pgc1α,Pparαand Prdm16 were significantly reduced in inguinal white adipose tissue(P<0.05).There was also a significant decrease in serumfgf21 levels due to the inhibition of Fgf21 expression in BAT(P<0.05).p38/MAPK signalling in brown adipose tissue was also significantly inhibited within brown adipose tissue leading to decreased fgf21 expression and secretion.CONCLUSION Our study shows that miR-32 plays a crucial role in stimulating beige cell emergence by activating p38/MAPK signalling during cold thermogenesis.miR-32 may prove effective as a treatment for obesity by activating cold-induced thermogenesis leading to increased energy metabolism.
文摘Various lignocellulosic materials such as wood, agricultural and forest residues has the potential to be valuable substitute for, or complement to, commercial sorbents for removing heavy metal ions or dyes from waste water or spilled oil from inland water or sea. More than 9 million tons of straw pulp are produced annually in china, which account for about 90% of the world’s total straw pulp. However, huge quantity of remain straw is not used as industrial raw material and is burnt in the fields or on the side of road. These resources can be chemical modified such as acetylation. Modified straws have the characteristics of low cost, high capacity, quick uptake, and easy to desorb. This paper reviews the current status of the technology for modified agricultural residues, which focus on hemicellulose and cellulose. The potential of these natural sorbents in main industry is also indicated.
文摘Both dependent and independent model algorithms are designed with genetic algorithm (GA) to retrieve aerosol size data from multispectral extinction measurements. Compared with the traditional dependent model algorithm, e.g., simplex, GA can locate the global optimized solution instead of local ones. As an independent model algorithm, when combined with B-splines, GA gives consistent results with Chahine and Phillip-Twomey-NNLS algorithms. Numerical simulations also show that GA has high stability and good resistance to relatively higher error levels. For a population size of 50 in the present paper, the feasible ranges for genetic operators pc and pm are found to be [0.01, 0.5] and [0.01, 0.15], respectively, and the generation number Gen_Max should be larger than 250.
