OBJECTIVE Doxorubicin-based therapy has been found to be not significantly effective for the treatment of advanced stage hepatocellular carcinomas(HCCs),which often undergo epithelial-mesenchymal transition(EMT)during...OBJECTIVE Doxorubicin-based therapy has been found to be not significantly effective for the treatment of advanced stage hepatocellular carcinomas(HCCs),which often undergo epithelial-mesenchymal transition(EMT)during tumor progression.Increasing evidence suggest(s)that epithelial cell transformation to mesenchymal state canenhance the ability to self-renew and confer greater resistance to the conventional chemotherapeutic drugs.The aim of this study was to examine the potential efficacyof ascochlorin,an isoprenoid antibiotic to overcome drug resistance induced by doxorubicin in HCC cell lines and to elucidate its underlying mechanism(s)of action.METHODS The effect of doxorubicin and ascochlorin on HCC cell lines was determined by MTT,Western blotting,immunofluorescence and NF-кB DNA binding assays.RESULTS Our results indicate that HCC cells that show a mesenchymal-like phenotype,are resistance to the doxorubicin therapy which directly correlated with an increased slug expression.We also observed that activation of NF-кB pathway plays an essential role in doxorubicin induced-chemoresistance and pharmacological inhibition of this pathway with ascochlorin can significantly reverse drug-induced invasion/migration and resistance in HCC cells.CONCLUSION Our results indicate that combination treatment of doxorubicin with ascochlorin has the potential to inhibit HCC growth and metastasis.展开更多
Polyoxometalates (POMs) α-K8 H6 [Si2W18Ti6O77] (Si2W18Ti6) loaded starch nanop-articles have been prepared and structurally characterized by elemental analyses, IR spectra and 29Si spectroscopy. The particle size of ...Polyoxometalates (POMs) α-K8 H6 [Si2W18Ti6O77] (Si2W18Ti6) loaded starch nanop-articles have been prepared and structurally characterized by elemental analyses, IR spectra and 29Si spectroscopy. The particle size of Si2W18Ti6 /starch was estimated by a Transmission electron microscope (TEM) and a 1000HSA MALVIRN Zetasizer instrument. The result shows that the polyoxometalate retained the parent structure after encapsulated by starch microspheres.展开更多
Objective: to observe the effect of sodium hyaluronate eye drops combined with flumirone eye drops in the treatment of children with dry eye. Methods: 120 children with dry eye in our hospital were divided into contro...Objective: to observe the effect of sodium hyaluronate eye drops combined with flumirone eye drops in the treatment of children with dry eye. Methods: 120 children with dry eye in our hospital were divided into control group (60 cases: treated with sodium hyaluronate eye drops) and experimental group (60 cases: treated with sodium hyaluronate eye drops combined with fluoromilone eye drops) according to whether sodium hyaluronate eye drops combined with fluoromilone eye drops were used. The therapeutic effects of the two groups were compared. Results: after two courses of treatment, the total effective rate of the experimental group (97.22%) was significantly higher than that of the control group (P < 0.05). The incidence of adverse reactions in the experimental group (11.12%) was slightly higher than that in the control group (P > 0.05). Conclusion: the therapeutic effect of sodium hyaluronate eye drops combined with flumirone eye drops in children with dry eye is significantly better than that of sodium hyaluronate eye drops.展开更多
Objective Glioma is a highly lethal tumor of the central nervous system(CNS)with limited therapeutic options.Recent evidence has highlighted the role of dysregulated alternative splicing in glioma progression.Although...Objective Glioma is a highly lethal tumor of the central nervous system(CNS)with limited therapeutic options.Recent evidence has highlighted the role of dysregulated alternative splicing in glioma progression.Although OY-TES-1 has been proposed as a potential therapeutic target,its splice isoforms have not been fully characterized.This study aimed to identify the clinically relevant splice variant of OY-TES-1 associated with glioma progression and to evaluate its potential as a target for innovative therapeutic strategies against this challenging disease.Method The potential splicing patterns of OY-TES-1,along with their relative frequency and correlation with patient sur-vival,were analyzed via the TCGA SpliceSeq and OncoSplicing databases.RNA-Seq by expectation maximization(RSEM)values and clinicopathological data for all OY-TES-1 gene transcripts were downloaded from the UCSC Xena database,and Cox regression analysis was performed for both univariate and multivariate prognostic assessments.The expression of OY-TES-1 mRNA in glioma and normal brain tissues was detected via RT-PCR.The relationships between OY-TES-1 mRNA expression and the clinicopathological characteristics of glioma patients were analyzed via the x2 test.OY-TES-1-V5a was overexpressed in glioma cells through transient transfection with plasmids as well as stable transfection with lentivirus for further functional analysis.Glioma cell proliferation was assessed via the Cell Counting Kit-8(CCK-8)assay.Migration and invasion abilities were evaluated via wound healing,Transwell,and Transwell Matrigel assays.Apoptosis was analyzed by flow cytometry.Results Bioinformatic analysis revealed four alternative splice variants of OY-TES-1 in glioma,among which OY-TES-1-V5a presented a relatively high percent spliced-in(PSI)value that was associated with significantly shorter overall survival.OY-TES-1-V5a was further identified as an independent prognostic risk factor for glioma patients,as its mRNA expression was significantly associated with Karnofsky performance status(KPS),tumor grade,and isocitrate dehydrogenase 1(IDH1)mutation status.RT-PCR validation confirmed that OY-TES-1-V5a was overexpressed in glioma tissues compared with normal brain tissues.Functionally,forced expression of OY-TES-1-V5a enhanced glioma cell proliferation,migration,and invasion while suppressing apoptosis.Conclusions The OY-TES-1 splice variant V5a is highly expressed in glioma,is associated with poor prognosis,and actively drives malignant behavior,indicating its potential utility as a prognostic biomarker and a candidate target for therapeutic intervention.展开更多
Differential proteome profiles of human lung squamous carcinoma tissue compared to paired tumor-adjacent normal bronchial epithelial tissue were established and analyzed by means of immobilized pH gradient-based two-d...Differential proteome profiles of human lung squamous carcinoma tissue compared to paired tumor-adjacent normal bronchial epithelial tissue were established and analyzed by means of immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and matrix-assisted laser desorp-tion/ionization time of flight mass spectrometry (MALDI-TOF-MS). The results showed that well-resolved, reproducible 2-DE patterns of human lung squamous carcinoma and adjacent normal bronchial epithelial tissues were obtained under the condition of 0.75-mg protein-load. The average deviation of spot position was 0.733±0.101 mm in IEF direction, and 0.925±0.207 mm in SDS-PAGE direction. For tumor tissue, a total of 1241±88 spots were detected, 987±65 spots were matched with an average matching rate of 79.5%. For control, a total of 1190±72 spots were detected, and 875±48 spots were matched with an average matching rate of 73.5%. A total of 864±34 spots were matched between tumors and controls. Forty-three differential proteins were characterized: some proteins were related to oncogenes, and others involved in the regulation of cell cycle and signal transduc-tion. It is suggested that the differential proteomic approach is valuable for mass identification of differentially expressed proteins involved in lung carcinogenesis. These data will be used to establish human lung cancer proteome database to further study human lung squamous carcinoma.展开更多
基金The project supported in part by agrant from National Medical Research Council of Singapore
文摘OBJECTIVE Doxorubicin-based therapy has been found to be not significantly effective for the treatment of advanced stage hepatocellular carcinomas(HCCs),which often undergo epithelial-mesenchymal transition(EMT)during tumor progression.Increasing evidence suggest(s)that epithelial cell transformation to mesenchymal state canenhance the ability to self-renew and confer greater resistance to the conventional chemotherapeutic drugs.The aim of this study was to examine the potential efficacyof ascochlorin,an isoprenoid antibiotic to overcome drug resistance induced by doxorubicin in HCC cell lines and to elucidate its underlying mechanism(s)of action.METHODS The effect of doxorubicin and ascochlorin on HCC cell lines was determined by MTT,Western blotting,immunofluorescence and NF-кB DNA binding assays.RESULTS Our results indicate that HCC cells that show a mesenchymal-like phenotype,are resistance to the doxorubicin therapy which directly correlated with an increased slug expression.We also observed that activation of NF-кB pathway plays an essential role in doxorubicin induced-chemoresistance and pharmacological inhibition of this pathway with ascochlorin can significantly reverse drug-induced invasion/migration and resistance in HCC cells.CONCLUSION Our results indicate that combination treatment of doxorubicin with ascochlorin has the potential to inhibit HCC growth and metastasis.
文摘Polyoxometalates (POMs) α-K8 H6 [Si2W18Ti6O77] (Si2W18Ti6) loaded starch nanop-articles have been prepared and structurally characterized by elemental analyses, IR spectra and 29Si spectroscopy. The particle size of Si2W18Ti6 /starch was estimated by a Transmission electron microscope (TEM) and a 1000HSA MALVIRN Zetasizer instrument. The result shows that the polyoxometalate retained the parent structure after encapsulated by starch microspheres.
文摘Objective: to observe the effect of sodium hyaluronate eye drops combined with flumirone eye drops in the treatment of children with dry eye. Methods: 120 children with dry eye in our hospital were divided into control group (60 cases: treated with sodium hyaluronate eye drops) and experimental group (60 cases: treated with sodium hyaluronate eye drops combined with fluoromilone eye drops) according to whether sodium hyaluronate eye drops combined with fluoromilone eye drops were used. The therapeutic effects of the two groups were compared. Results: after two courses of treatment, the total effective rate of the experimental group (97.22%) was significantly higher than that of the control group (P < 0.05). The incidence of adverse reactions in the experimental group (11.12%) was slightly higher than that in the control group (P > 0.05). Conclusion: the therapeutic effect of sodium hyaluronate eye drops combined with flumirone eye drops in children with dry eye is significantly better than that of sodium hyaluronate eye drops.
基金supported by grants from the Natural Science Foundation of Guangxi Province(Grant No.2023GXNSFAA026120 and 2023GXNSFBA026092)the National Natural Science Foundation of China(Grant No.82260608 and 82260554)+1 种基金the Key Laboratory of Basic Research on Regional Diseases(Guangxi Medical University),Education Department of Guangxi Zhuang Autonomous Region(Grant No.RTFY202302)Innovation Project of Guangxi Graduate Education(Grant No.YCSW2025248 and YCSW2024248).
文摘Objective Glioma is a highly lethal tumor of the central nervous system(CNS)with limited therapeutic options.Recent evidence has highlighted the role of dysregulated alternative splicing in glioma progression.Although OY-TES-1 has been proposed as a potential therapeutic target,its splice isoforms have not been fully characterized.This study aimed to identify the clinically relevant splice variant of OY-TES-1 associated with glioma progression and to evaluate its potential as a target for innovative therapeutic strategies against this challenging disease.Method The potential splicing patterns of OY-TES-1,along with their relative frequency and correlation with patient sur-vival,were analyzed via the TCGA SpliceSeq and OncoSplicing databases.RNA-Seq by expectation maximization(RSEM)values and clinicopathological data for all OY-TES-1 gene transcripts were downloaded from the UCSC Xena database,and Cox regression analysis was performed for both univariate and multivariate prognostic assessments.The expression of OY-TES-1 mRNA in glioma and normal brain tissues was detected via RT-PCR.The relationships between OY-TES-1 mRNA expression and the clinicopathological characteristics of glioma patients were analyzed via the x2 test.OY-TES-1-V5a was overexpressed in glioma cells through transient transfection with plasmids as well as stable transfection with lentivirus for further functional analysis.Glioma cell proliferation was assessed via the Cell Counting Kit-8(CCK-8)assay.Migration and invasion abilities were evaluated via wound healing,Transwell,and Transwell Matrigel assays.Apoptosis was analyzed by flow cytometry.Results Bioinformatic analysis revealed four alternative splice variants of OY-TES-1 in glioma,among which OY-TES-1-V5a presented a relatively high percent spliced-in(PSI)value that was associated with significantly shorter overall survival.OY-TES-1-V5a was further identified as an independent prognostic risk factor for glioma patients,as its mRNA expression was significantly associated with Karnofsky performance status(KPS),tumor grade,and isocitrate dehydrogenase 1(IDH1)mutation status.RT-PCR validation confirmed that OY-TES-1-V5a was overexpressed in glioma tissues compared with normal brain tissues.Functionally,forced expression of OY-TES-1-V5a enhanced glioma cell proliferation,migration,and invasion while suppressing apoptosis.Conclusions The OY-TES-1 splice variant V5a is highly expressed in glioma,is associated with poor prognosis,and actively drives malignant behavior,indicating its potential utility as a prognostic biomarker and a candidate target for therapeutic intervention.
基金This work was supported by a grant from National 973 Project (2001CB5102) for Outstanding Scholars of New Era from the Ministry of Education of China (2002-48)+1 种基金 National Natural Science Foundation of China (30000028) the key research program from Sc
文摘Differential proteome profiles of human lung squamous carcinoma tissue compared to paired tumor-adjacent normal bronchial epithelial tissue were established and analyzed by means of immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and matrix-assisted laser desorp-tion/ionization time of flight mass spectrometry (MALDI-TOF-MS). The results showed that well-resolved, reproducible 2-DE patterns of human lung squamous carcinoma and adjacent normal bronchial epithelial tissues were obtained under the condition of 0.75-mg protein-load. The average deviation of spot position was 0.733±0.101 mm in IEF direction, and 0.925±0.207 mm in SDS-PAGE direction. For tumor tissue, a total of 1241±88 spots were detected, 987±65 spots were matched with an average matching rate of 79.5%. For control, a total of 1190±72 spots were detected, and 875±48 spots were matched with an average matching rate of 73.5%. A total of 864±34 spots were matched between tumors and controls. Forty-three differential proteins were characterized: some proteins were related to oncogenes, and others involved in the regulation of cell cycle and signal transduc-tion. It is suggested that the differential proteomic approach is valuable for mass identification of differentially expressed proteins involved in lung carcinogenesis. These data will be used to establish human lung cancer proteome database to further study human lung squamous carcinoma.
