This study aimed to develop bioactive films based on curdlan(CD)and sodium alginate(SA)to enhance the shelf life of the Volvariella volvacea mushroom.A stable nanoemulsion of droplet size of 150.1±5 nm was first ...This study aimed to develop bioactive films based on curdlan(CD)and sodium alginate(SA)to enhance the shelf life of the Volvariella volvacea mushroom.A stable nanoemulsion of droplet size of 150.1±5 nm was first formulated using 5%soy protein(Sp)and 10%lavender essential oil(LEO),achieved by ultrasound treatment for 10 min,resulting in small droplet sizes.In parallel,a CD-SA film was prepared by mixing them in a 1:1 ratio.The SpLEO nanoemulsion was then ex situ added to the CD-SA film at varying concentrations(4%,6%,8%,and 10%).The resulting bioactive film exhibited desirable properties,including water vapor permeability(1.15±0.05×10^(-10)g m^(-1)Pa^(-1)s^(-1)),swelling rate(547%),and tensile strength(10.1±1.3 MPa).Docking and structural analyses suggested that ultrasound treatment enhanced the intermolecular hydrogen bonding,creating a uniform and regular surface and reduced crystallinity.The CD-SA film with ultrasound-treated 8%SpLEO nanoemulsion[i.e.,(CD-SA/SpLEO-8)US]showed antibacterial activity against Escherichia coli and Staphylococcus aureus and extended the freshness of straw mushrooms prevention of spoilage during storage.Additionally,the film reduced respiration rate(975 mg CO_(2)/kg/h at 96 h)and ethylene production(160 ng/kg/s by 96 h)while effectively inhibiting straw mushroom autolysis.This led to a reduction in browning(2.91),total soluble solids(7.5%),weight loss(13.9%),and polyphenol oxidase activity(0.08ΔOD_(420)/min⋅g),thereby extending the shelf life of the mushrooms to 96 h.These findings highlight the potential of incorporating different molecules using ultrasound treatment to develop innovative edible films for food preservation.展开更多
Grifola frondosa is a highly valuable edible and medicinal fungus with significant health benefits.In traditional Chinese medicine,has been extensively utilized for its therapeutic properties,playing a vital role in t...Grifola frondosa is a highly valuable edible and medicinal fungus with significant health benefits.In traditional Chinese medicine,has been extensively utilized for its therapeutic properties,playing a vital role in treating various diseases related to immune modulation and inflammation.Polysaccharides are the main active com-pound in G.frondosa,and their biological activities are closely related to their composition.The Grifola frondosa polysaccharides(GFPs)interact with key immune receptors such as Toll-like receptors and Dectin-1,which activate macrophages,natural killer cells,and dendritic cells.This interaction enhances the immune system’s capacity to mount effective responses against infections and malignancies.These polysaccharides control glucose metabolism and regulate insulin sensitivity,indicating their potential for managing and preventing metabolic disorders like diabetes.Although the preclinical studies are promising,further research is essential to standardize extraction techniques,conform clinical efficacy,and clarify the mechanism of health benefits.This review provides a comprehensive overview of recent advances in the production,properties,structural characteristics,and biological activities of GFPs.It highlights their therapeutic potential and promotes further research to support their development and application in functional foods,nutraceuticals,and pharmaceutical formulation.展开更多
Ganoderma lucidum is a well-known functional food-grade and medicinal mushroom with 2000-year history for promoting health and longevity in Asian countries.G.lucidum polysaccharides are the most investigated biologica...Ganoderma lucidum is a well-known functional food-grade and medicinal mushroom with 2000-year history for promoting health and longevity in Asian countries.G.lucidum polysaccharides are the most investigated biological components due to their attracting nutritional and medicinal values.The increasing efforts have also attempted to understand the biosynthesis pathways of G.lucidum polysaccharides and the roles of involved key genes based on the recent rapid advances and increasing availability in G.lucidum genome.On this basis,the present study presented the updated progresses on structural characteristics,bioactivities/potential mechanisms,and complex biosynthesis process/associated key genes of G.lucidum polysaccharides(GLPs)in the past 5 years.The recent process optimization and genetic engineering to maximally produce GLPs were also summarized.The future trends and challenges to answer the structure-activity relationship,biosynthesis and molecular regulation of GLPs are also proposed.展开更多
This study conducted a comparative analysis to characterize crude polysaccharides from seven selected strains of Auricularia cornea in China,including commercial,hybrid,and wild domesticated strains.The obtained crude...This study conducted a comparative analysis to characterize crude polysaccharides from seven selected strains of Auricularia cornea in China,including commercial,hybrid,and wild domesticated strains.The obtained crude polysaccharide samples were analyzed using electron microscope-energy dispersive X-ray(SEM-EDX),Fourier transform infrared(FTIR)spectroscopy,and for their monosaccharide composition.The results revealed that crude polysaccharide samples were mainly composed of 85.9%mannose and 12.3%glucose.Among the strains tested,Ac1 and Ac33 exhibited significantly higher total polysaccharide content(215.9±2.4 mg/g and 200.3±1.5 mg/g,respectively)and phenolic content(40.2±4.0 mg GAE/g and 44.2±2.7 mg GAE/g,respectively).Additionally,Ac33 and Ac24 showed the highest levels of DPPH radical scavenging and ABTS scavenging ability among the strains tested.Overall,the results suggest that the crude polysaccharides from A.cornea have the potential as a natural source of antioxidant and antimicrobial agents and could be developed into functional nutritional foods.展开更多
基金supported by funding from the Agriculture Research System of Shanghai(Grant No.202209)the Agricultural Field Project of Shanghai"Action Plan for Scientific and Technological Innovation"(23N31900400)+1 种基金the Metasequoia Teacher Research Start-up Fund(163105998)the Researchers Supporting Project number(RSP2024R98),King Saud University,Riyadh,Saudi Arabia,for financial support.
文摘This study aimed to develop bioactive films based on curdlan(CD)and sodium alginate(SA)to enhance the shelf life of the Volvariella volvacea mushroom.A stable nanoemulsion of droplet size of 150.1±5 nm was first formulated using 5%soy protein(Sp)and 10%lavender essential oil(LEO),achieved by ultrasound treatment for 10 min,resulting in small droplet sizes.In parallel,a CD-SA film was prepared by mixing them in a 1:1 ratio.The SpLEO nanoemulsion was then ex situ added to the CD-SA film at varying concentrations(4%,6%,8%,and 10%).The resulting bioactive film exhibited desirable properties,including water vapor permeability(1.15±0.05×10^(-10)g m^(-1)Pa^(-1)s^(-1)),swelling rate(547%),and tensile strength(10.1±1.3 MPa).Docking and structural analyses suggested that ultrasound treatment enhanced the intermolecular hydrogen bonding,creating a uniform and regular surface and reduced crystallinity.The CD-SA film with ultrasound-treated 8%SpLEO nanoemulsion[i.e.,(CD-SA/SpLEO-8)US]showed antibacterial activity against Escherichia coli and Staphylococcus aureus and extended the freshness of straw mushrooms prevention of spoilage during storage.Additionally,the film reduced respiration rate(975 mg CO_(2)/kg/h at 96 h)and ethylene production(160 ng/kg/s by 96 h)while effectively inhibiting straw mushroom autolysis.This led to a reduction in browning(2.91),total soluble solids(7.5%),weight loss(13.9%),and polyphenol oxidase activity(0.08ΔOD_(420)/min⋅g),thereby extending the shelf life of the mushrooms to 96 h.These findings highlight the potential of incorporating different molecules using ultrasound treatment to develop innovative edible films for food preservation.
基金supported by funding from Natural Science Founda-tion of China(32172187,31771961)Chinese Postdoctoral Science Foundation(2023M731377,2023M661761)Science&Technology Platform Construction Program of Jiangxi Province.
文摘Grifola frondosa is a highly valuable edible and medicinal fungus with significant health benefits.In traditional Chinese medicine,has been extensively utilized for its therapeutic properties,playing a vital role in treating various diseases related to immune modulation and inflammation.Polysaccharides are the main active com-pound in G.frondosa,and their biological activities are closely related to their composition.The Grifola frondosa polysaccharides(GFPs)interact with key immune receptors such as Toll-like receptors and Dectin-1,which activate macrophages,natural killer cells,and dendritic cells.This interaction enhances the immune system’s capacity to mount effective responses against infections and malignancies.These polysaccharides control glucose metabolism and regulate insulin sensitivity,indicating their potential for managing and preventing metabolic disorders like diabetes.Although the preclinical studies are promising,further research is essential to standardize extraction techniques,conform clinical efficacy,and clarify the mechanism of health benefits.This review provides a comprehensive overview of recent advances in the production,properties,structural characteristics,and biological activities of GFPs.It highlights their therapeutic potential and promotes further research to support their development and application in functional foods,nutraceuticals,and pharmaceutical formulation.
基金supported by funding from Natural Science Foundation of China(32172187,31771961)China Postdoctoral Science Foundation(2023M731377)Science&Technology Platform Construction Program of Jiangxi Province.
文摘Ganoderma lucidum is a well-known functional food-grade and medicinal mushroom with 2000-year history for promoting health and longevity in Asian countries.G.lucidum polysaccharides are the most investigated biological components due to their attracting nutritional and medicinal values.The increasing efforts have also attempted to understand the biosynthesis pathways of G.lucidum polysaccharides and the roles of involved key genes based on the recent rapid advances and increasing availability in G.lucidum genome.On this basis,the present study presented the updated progresses on structural characteristics,bioactivities/potential mechanisms,and complex biosynthesis process/associated key genes of G.lucidum polysaccharides(GLPs)in the past 5 years.The recent process optimization and genetic engineering to maximally produce GLPs were also summarized.The future trends and challenges to answer the structure-activity relationship,biosynthesis and molecular regulation of GLPs are also proposed.
基金supported by China Agriculture Research System(No.CARS-20)National Key Research and Development Program of China(2023YFD1201604-4)+2 种基金the project of“one thousand talents plan”in Jiangxi Province,Chinathe collaborative project on studying the domestication and selection of edible and medicinal fungi resources in Zambia between Jilin Agricultural University and University of Zambiasupporting this work through project(No.TU-DSPP-2024-186).
文摘This study conducted a comparative analysis to characterize crude polysaccharides from seven selected strains of Auricularia cornea in China,including commercial,hybrid,and wild domesticated strains.The obtained crude polysaccharide samples were analyzed using electron microscope-energy dispersive X-ray(SEM-EDX),Fourier transform infrared(FTIR)spectroscopy,and for their monosaccharide composition.The results revealed that crude polysaccharide samples were mainly composed of 85.9%mannose and 12.3%glucose.Among the strains tested,Ac1 and Ac33 exhibited significantly higher total polysaccharide content(215.9±2.4 mg/g and 200.3±1.5 mg/g,respectively)and phenolic content(40.2±4.0 mg GAE/g and 44.2±2.7 mg GAE/g,respectively).Additionally,Ac33 and Ac24 showed the highest levels of DPPH radical scavenging and ABTS scavenging ability among the strains tested.Overall,the results suggest that the crude polysaccharides from A.cornea have the potential as a natural source of antioxidant and antimicrobial agents and could be developed into functional nutritional foods.
