OBJECTIVE:To assess the acute organ toxicity of Strychnos nux-vomica with zebrafish model visually.METHODS:To assess acute toxicity,we initially determined the lethal concentration after Strychnos nux-vomica treatment...OBJECTIVE:To assess the acute organ toxicity of Strychnos nux-vomica with zebrafish model visually.METHODS:To assess acute toxicity,we initially determined the lethal concentration after Strychnos nux-vomica treatment for 24 h.Zebrafish was treated with five concentrations Strychnos nux-≦vo LC10 for 24 h,and the effects ofmica on morphology,function of heart,central nervous system,liver,kidney and organ-specific cell death were assessed.Next,we assessed the reversibility of toxic effect.RESULTS:Strychnos nux-vomica has an effect on the different organs of zebrafish,including heart,central nervous system,liver,and kidney,and cadiotoxicity induced by Strychnos nux-vomica was reversible to some extent.CONCLUSION:Zebrafish model is suitable for confirming the toxic target organs for Chinese traditional medicine.展开更多
文摘目的研究金荞麦(Fagopyri Dibotryis Rhizoma,FDR)从饮片到标准汤剂的关键质量属性传递规律。方法收集15批FDR饮片,制备标准汤剂,测定出膏率,建立饮片和标准汤剂的高效液相色谱(HPLC)指纹图谱,采用超快速液相色谱-四级杆飞行时间质谱(ultra fast liquid chromatography quadrupole time-of-flight mass spectrometry,UFLC-Q-TOF-MS/MS)技术对标定的共有峰进行成分鉴定,HPLC法测定其中10个指标性成分的含量,以出膏率、指纹图谱共有峰传递数、指标性成分转移率为关键质量属性,分析从饮片到标准汤剂的传递规律。结果FDR饮片指纹图谱标定共有峰56个,其中38个传递到了标准汤剂,共有峰个数传递率67.86%,56个共有峰共鉴定了93个成分,包括鞣质类成分33个,酚类成分22个,黄酮类成分、苯丙素苷类成分各14个,氨基酸类成分4个,有机酸类成分3个,萜类成分、生物碱类成分及其他类成分各1个,其中6个鞣质类成分(原花青素A2、原花青素B1、原花青素B2、原花青素B3、原花青素C1和原花青素C2)、3个酚类成分(原儿茶酸、没食子酸和原儿茶醛)、4个黄酮类成分(儿茶素、表儿茶素、表儿茶素没食子酸酯和芦丁)采用对照品比对确认,原儿茶酸、没食子酸、原儿茶醛、原花青素A2、原花青素B1、原花青素B3、原花青素C1、儿茶素、表儿茶素、表儿茶素没食子酸酯平均转移率分别为115.50%、81.36%、126.36%、36.86%、69.60%、47.15%、43.10%、86.78%、69.00%、16.18%,标准汤剂的出膏率范围为6.36%~10.61%。结论本研究为FDR配方颗粒制备工艺和质量标准研究奠定了基础。
基金Supported by the Scientific Research And Postgraduate Education Joint Project(Key Technology Research and Application of Safety Evaluation for Toxic Chinese Medicinal Materials on the Basis of the Characteristics of Chemical Composition and Zebrafish,No.2050205)
文摘OBJECTIVE:To assess the acute organ toxicity of Strychnos nux-vomica with zebrafish model visually.METHODS:To assess acute toxicity,we initially determined the lethal concentration after Strychnos nux-vomica treatment for 24 h.Zebrafish was treated with five concentrations Strychnos nux-≦vo LC10 for 24 h,and the effects ofmica on morphology,function of heart,central nervous system,liver,kidney and organ-specific cell death were assessed.Next,we assessed the reversibility of toxic effect.RESULTS:Strychnos nux-vomica has an effect on the different organs of zebrafish,including heart,central nervous system,liver,and kidney,and cadiotoxicity induced by Strychnos nux-vomica was reversible to some extent.CONCLUSION:Zebrafish model is suitable for confirming the toxic target organs for Chinese traditional medicine.
