Edible oils derived from aquatic products are rich in lipids beneficial to human health.However,the volatile flavor characteristics of flesh oil and liver oil from Doederleinia berycoides remain unclear.In this study,...Edible oils derived from aquatic products are rich in lipids beneficial to human health.However,the volatile flavor characteristics of flesh oil and liver oil from Doederleinia berycoides remain unclear.In this study,flesh oil and liver oil were extracted from Doederleinia berycoides,revealing different fatty acid compositions and contents.Lipidomics analysis identified a total of 124 differential lipids between the flesh oil and liver oil,including 42 glycerophospholipids(GPs),33 glycerolipids(GLs),23 free fatty acids(FAs),13 sphingolipids(SPs),10 sterols(STs),and 3 prenol lipids(PRs).Analysis using HS-GC-IMS identified 12 key volatile compounds that significantly contributed to the distinct volatile flavors of the flesh and liver oils.The volatile flavors originated from these volatile compounds,which had different Relative Odor Activity Values(ROAVs).Further results from HSSPME-GC-MS showed that the volatile flavors of the flesh oil and liver oil were respectively attributed to 64 and 35 volatile compounds,each with unique key volatile compounds exhibiting different ROAVs.There were significant positive or negative correlations between 18 key differential lipids and 24 volatile compounds in both flesh oil and liver oil.Therefore,the complex lipid profiles are responsible for the unique volatile flavors of flesh oil and liver oil,and the differential lipids play a central role in their volatile flavor formation.These findings provide a foundation for understanding the volatile flavor differences in fish oils and hold promise for further exploration of the molecular mechanisms underlying oil volatile flavors.展开更多
Cardiovascular disease(CVD)is a major global health challenge,which causes significant illness and death worldwide.These include a range of conditions that affect the heart and blood vessels,including coro-nary artery...Cardiovascular disease(CVD)is a major global health challenge,which causes significant illness and death worldwide.These include a range of conditions that affect the heart and blood vessels,including coro-nary artery disease,stroke,peripheral artery disease,and heart failure.Despite advances in medicine and healthcare delivery,CVD continues to have a serious impact on individuals,families,and the healthcare system.This review begins by delineating the merits and demerits of commonly employed synthetic and natural materials for artificial blood vessels.It delves into various techniques commonly employed in the fabrication of artificial blood vessels,encompassing advanced textile technologies,electrospinning,ther-mally induced phase separation,and 3D printing.The review critically analyzes the attributes of different preparation methodologies alongside the latest advancements in research.The review also outlines the requisite performance requirements for artificial blood vessels,which encompass robust mechanical prop-erties,appropriate porosity,exceptional compatibility,and antibacterial attributes.It provides a succinct overview of ongoing effort s in vascular functionalization,particularly emphasizing thrombus mitigation,promotion of endothelialization,and enhancement of nitric oxide production.The review finally encap-sulates the primary challenges confronting vascular grafts and prospective avenues for future research.展开更多
Intercropping has been widely used in arid and semi-arid regions because of its high yield,stable productivity,and efficient utilization of resources.However,in recent years,the high yield of traditional intercropping...Intercropping has been widely used in arid and semi-arid regions because of its high yield,stable productivity,and efficient utilization of resources.However,in recent years,the high yield of traditional intercropping is mainly attributed to the large amount of purchased resources such as water and fertilizer,plastic film,and mechanical power.These lead to a decline in cultivated land quality and exacerbate intercrops'premature root and canopy senescence.So,the application of traditional intercropping faces major challenges in crop production.This paper analyzes the manifestations,occurrence mechanisms,and agronomic regulatory pathways of crop senescence.The physiological and ecological characteristics of intercropping to delay root and canopy senescence of crops are reviewed in this paper.The main agronomic regulatory pathways of intercropping to delay root and canopy senescence of crops are based on above-and blow-ground interactions,including collocation of crop varieties,spatial arrangement,water and fertilizer management,and tillage and mulch practices.Future research fields of intercropping to delay root and canopy senescence should focus on the aspects of selecting and breeding special varieties,application of molecular biology techniques,and developing or applying models to predict and evaluate the root and canopy senescence process of intercrops.Comprehensive analysis and evaluation of different research results could provide a basis for enhancing intercropping delay root and canopy senescence through adopting innovative technologies for regulating the physio-ecological characteristics of intercrops.This would support developing and adopting high-yield,efficient,and sustainable intercropping systems in arid and semi-arid areas with high population density,limited land,and abundant light and heat resources.展开更多
BACKGROUND Cleidocranial dysplasia(CCD)is an infrequent clinical condition with an autosomal dominant inheritance pattern.It is characterized by abnormal clavicles,patent sutures and fontanelles,supernumerary teeth,an...BACKGROUND Cleidocranial dysplasia(CCD)is an infrequent clinical condition with an autosomal dominant inheritance pattern.It is characterized by abnormal clavicles,patent sutures and fontanelles,supernumerary teeth,and short stature.Approximately 60%-70%of patients with CCD have mutations in the RUNX family transcription factor 2 gene.However,prenatal diagnosis of CCD is difficult when the family history is unknown.CASE SUMMARY We report a rare case of fetal CCD with an unknown family history,confirmed by prenatal ultrasonography and genetic testing at a gestational age of 16 weeks.The genetic reports indicated that the fetus carried pathogenic mutations in the RUNX family transcription factor 2 gene(c.674G>A).After careful consideration,the pregnant woman and her family decided to continue the pregnancy.CONCLUSION Definitive prenatal diagnosis of CCD should include family history,ultrasound diagnosis,and genetic analysis,especially if family history is unknown.展开更多
Abstract The dopamine transporter （DAT） is involved in the regulation of extracellular dopamine levels. A 40-bp variable-number tandem repeat （VNTR） polymorphism in the 3-untranslated region （3UTR） of the DAT ha...Abstract The dopamine transporter （DAT） is involved in the regulation of extracellular dopamine levels. A 40-bp variable-number tandem repeat （VNTR） polymorphism in the 3-untranslated region （3UTR） of the DAT has been reported to be associated with various phenotypes that are involved in the aberrant regulation of dopaminergic neu- rotransmission. In the present study, we found that miR- 137 and miR-491 caused a marked reduction of DAT expression, thereby influencing neuronal dopamine trans- port. Moreover, the regulation of miR-137 and miR-491 on this transport disappeared after the DAT was silenced. The miR-491 seed region that is located on the VNTR sequence in the 3＇UTR of the DAT and the regulatory effect of miR- 491 on the DAT depended on the VNTR copy-number. These data indicate that miR-137 and miR-491 regulate DAT expression and dopamine transport at the post- transcriptional level, suggesting that microRNA may be targeted for the treatment of diseases associated with DAT dysfunction.展开更多
A sky model from CLEAN deconvolution is a particularly effective high dynamic range reconstruction in radio astronomy,which can effectively model the sky and remove the sidelobes of the point spread function(PSF)cause...A sky model from CLEAN deconvolution is a particularly effective high dynamic range reconstruction in radio astronomy,which can effectively model the sky and remove the sidelobes of the point spread function(PSF)caused by incomplete sampling in the spatial frequency domain.Compared to scale-free and multi-scale sky models,adaptive-scale sky modeling,which can model both compact and diffuse features,has been proven to have better sky modeling capabilities in narrowband simulated data,especially for large-scale features in high-sensitivity observations which are exactly one of the challenges of data processing for the Square Kilometre Array(SKA).However,adaptive scale CLEAN algorithms have not been verified by real observation data and allow negative components in the model.In this paper,we propose an adaptive scale model algorithm with non-negative constraint and wideband imaging capacities,and it is applied to simulated SKA data and real observation data from the Karl G.Jansky Very Large Array(JVLA),an SKA precursor.Experiments show that the new algorithm can reconstruct more physical models with rich details.This work is a step forward for future SKA image reconstruction and developing SKA imaging pipelines.展开更多
BACKGROUND Gallbladder cancer is a highly malignant and aggressive tumor,often diagnosed at an advanced stage.The prognosis for advanced gallbladder cancer remains poor,with limited options for effective treatment.CAS...BACKGROUND Gallbladder cancer is a highly malignant and aggressive tumor,often diagnosed at an advanced stage.The prognosis for advanced gallbladder cancer remains poor,with limited options for effective treatment.CASE SUMMARY A 65-year-old male patient presented with a soft tissue mass in the gallbladder.Following laparoscopic exploration,he underwent radical surgery for gallbladder cancer,with the tumor staged as pT2N1M0(stage III).Post-surgery,the patient received four cycles of adjuvant chemotherapy.However,one month later,over 60 metastatic lesions were detected in the liver,lungs,and lymph nodes.In response to the widespread metastasis,he underwent six cycles of combination therapy consisting of sintilimab,albumin-bound paclitaxel,and cisplatin.After two cycles,a partial response was achieved.Maintenance therapy was initiated with a combination of sintilimab and albumin-bound paclitaxel for four cycles.Monotherapy with sintilimab was continued thereafter until October 2023.Complete remission was confirmed in September 2021.The patient sustained this complete response for more than three years,including an eleven-month period without disease progression following the discontinuation of sintilimab.Genetic analysis revealed a tumor mutational burden of 15.4 mutations/megabase,which indicates a potentially favorable response to immunotherapy.CONCLUSION This case demonstrates the potential of combining immunotherapy(sintilimab)with chemotherapy to achieve durable remission in metastatic gallbladder cancer,even in a patient with extensive metastasis.In addition,it indicated the importance of tumor mutational burden as a predictive biomarker of immunotherapy.展开更多
This study investigates how universities are developing normativeframeworks to regulate the use of generative AI tools in higher education, with aparticular focus on balancing empowerment and discipline. Drawing on th...This study investigates how universities are developing normativeframeworks to regulate the use of generative AI tools in higher education, with aparticular focus on balancing empowerment and discipline. Drawing on theoreticallenses such as Foucault’s discipline theory and contemporary AI ethics, the paperanalyzes policy documents from institutions including Harvard, Oxford, and severaltop Chinese universities. Using Latent Dirichlet Allocation (LDA) topic modeling,the study reveals five dominant governance themes—ranging from academicintegrity enforcement to pedagogical empowerment. The findings highlight a globalshift from restrictive to balanced, ethics-informed AI governance, with significantdisciplinary variations. The paper concludes by proposing a “principledpermissiveness” model that combines transparency, accountability, andpedagogical innovation in future AI governance.展开更多
Cleat serves as the primary flow pathway for coalbed methane(CBM)and water.However,few studies consider the impact of local contact on two-phase flow within cleats.A visual generalized model of endogenous cleats was c...Cleat serves as the primary flow pathway for coalbed methane(CBM)and water.However,few studies consider the impact of local contact on two-phase flow within cleats.A visual generalized model of endogenous cleats was constructed based on microfluidics.A microscopic and mesoscopic observation technique was proposed to simultaneously capture gas-liquid interface morphology of pores and throat and the two-phase flow characteristics in entire cleat system.The local contact characteristics of cleats reduced absolute permeability,which resulted in a sharp increase in the starting pressure.The reduced gas flow capacity narrowed the co-infiltration area and decreased water saturation at the isotonic point in a hydrophilic environment.The increased local contact area of cleats weakened gas phase flow capacity and narrowed the co-infiltration area.Jumping events occurred in methane-water flow due to altered porosity caused by local contact in cleats.The distribution of residual phases changed the jumping direction on the micro-scale as well as the dominant channel on the mesoscale.Besides,jumping events caused additional energy dissipation,which was ignored in traditional two-phase flow models.This might contribute to the overestimation of relative permeability.The work provides new methods and insights for investigating unsaturated flow in complex porous media.展开更多
Stomata are tiny pores on the plant leaf surface that regulate the exchange of water and gases between the plant and the external environment.They are crucial for photosynthesis,water use efficiency(WUE),and the plant...Stomata are tiny pores on the plant leaf surface that regulate the exchange of water and gases between the plant and the external environment.They are crucial for photosynthesis,water use efficiency(WUE),and the plant’s ability to adapt to environmental changes.Stomatal movement is vital for understanding how plants adapt to environmental stress and optimize resource utilization.Changes in environmental conditions,especially the quality and intensity of light throughout the day,affect stomatal dynamics and diurnal behavior,which in turn impact photosynthetic efficiency and water-use efficiency.In this review,we summarize the biophysical principles and mechanisms of stomatal movement regulated by ion transport at the plasma membrane,vacuolar membrane and metabolic activity through persulfidation or S-nitrosylation modifications.Specifically,we focus on recent progress in the regulation of stomatal movement by different light qualities,and summarize the photochemical and biochemical events underlying photoreceptors as well as the knowledge of novel regulatory functions and signaling in the multilayer control of stomatal movement and environmental adaptation.Furthermore,as rising global temperatures and increased water needs of farming methods are expected to escalate future crop losses,we explore the potential of smart LED lighting and gene editing technology in enhancing photosynthetic efficiency and water-use efficiency,leading to increased crop biomass and higher crop yields.展开更多
Inflammation represents a critical immune response triggered by cellular activities and inflammatory mediators following tissue damage.It plays a central role in the pathological progression of diverse diseases,includ...Inflammation represents a critical immune response triggered by cellular activities and inflammatory mediators following tissue damage.It plays a central role in the pathological progression of diverse diseases,including psychiatric disorders,cancer,and immunological conditions,rendering it an essential target for therapeutic intervention.Periodontitis,a prevalent oral inflammatory disease,is a leading cause of tooth loss and poses significant health challenges globally.Traditionally,inflammatory diseases such as periodontitis have been treated with systemic administration of synthetic chemicals.However,recent years have witnessed challenges,including drug resistance and microbial dysbiosis associated with these treatments.In contrast,natural products derived from Chinese medicine offer numerous benefits,such as high safety profiles,minimal side effects,innovative pharmacological mechanisms,ease of extraction,and multiple targets,rendering them viable alternatives to conventional antibiotics for treating inflammatory conditions.Numerous effective anti-inflammatory natural products have been identified in traditional Chinese medicine(TCM),including alkaloids,flavonoids,terpenoids,lignans,and other natural products that exhibit inhibitory effects on inflammation and are potential therapeutic agents.Several studies have confirmed the substantial anti-inflammatory and immunomodulatory properties of these compounds.This comprehensive review examines the literature on the anti-inflammatory effects of TCM-derived natural products from databases such as PubMed,Web of Science,and CNKI,focusing on terms like"inflammation","periodontitis","pharmacology",and"traditional Chinese medicine".The analysis systematically summarizes the molecular pharmacology,chemical composition,and biological activities of these compounds in inflammatory responses,alongside their mechanisms of action.This research seeks to deepen understanding of the mechanisms and biological activities of herbal extracts in managing inflammatory diseases,potentially leading to the development of promising new anti-inflammatory drug candidates.Future applications could extend to the treatment of various inflammatory conditions,including periodontitis.展开更多
FOXL 2 and CYP 19 B are crucial transcription factors in vertebrates and invertebrates that play pivotal roles in sex differentiation and gonadal development.The potential roles of the foxl 2 and cyp 19 b genes in sex...FOXL 2 and CYP 19 B are crucial transcription factors in vertebrates and invertebrates that play pivotal roles in sex differentiation and gonadal development.The potential roles of the foxl 2 and cyp 19 b genes in sex determination and gonadal development in Cyprinus carpio var.koi were explored using a non-invasive RNA interference(RNAi)method,histopathological observation and qPCR.Results demonstrate that foxl 2 exhibited a sexually dimorphic expression pattern in gonads,with a notable expression in ovaries;cyp 19 b was expressed in all peripheral tissues,with a particularly prominent expression in brain and gonads.The knockdown of foxl 2 by RNAi resulted in delay in the development of the female gonads.Conversely,no notable alterations were discerned in the gonads of C.carpio var.koi following the knockdown of cyp 19 b.The upregulation of sox 9 a,amh,and cyp 19 b following foxl 2 knockdown indicates that foxl 2 may play a pivotal role in gonadal development.Nevertheless,further investigation is required to ascertain the potential role of cyp 19 b.This study elucidated the role of foxl 2 and enhanced the understanding of the mechanisms of sex determination and gonadal development in C.carpio var.koi.展开更多
基金supported by the R&D Projects in Key Areas of Guangdong Province(2023B0202080003)the National Natural Science Foundation of China(32472272,32302135,32072291)+1 种基金“Pioneer”and“Leading Goose”R&D Program of Zhejiang(2023C02006)Youth S&T Talent Support Programme of Guangdong Provincial Association for Science and Technology(SKXRC202401)。
文摘Edible oils derived from aquatic products are rich in lipids beneficial to human health.However,the volatile flavor characteristics of flesh oil and liver oil from Doederleinia berycoides remain unclear.In this study,flesh oil and liver oil were extracted from Doederleinia berycoides,revealing different fatty acid compositions and contents.Lipidomics analysis identified a total of 124 differential lipids between the flesh oil and liver oil,including 42 glycerophospholipids(GPs),33 glycerolipids(GLs),23 free fatty acids(FAs),13 sphingolipids(SPs),10 sterols(STs),and 3 prenol lipids(PRs).Analysis using HS-GC-IMS identified 12 key volatile compounds that significantly contributed to the distinct volatile flavors of the flesh and liver oils.The volatile flavors originated from these volatile compounds,which had different Relative Odor Activity Values(ROAVs).Further results from HSSPME-GC-MS showed that the volatile flavors of the flesh oil and liver oil were respectively attributed to 64 and 35 volatile compounds,each with unique key volatile compounds exhibiting different ROAVs.There were significant positive or negative correlations between 18 key differential lipids and 24 volatile compounds in both flesh oil and liver oil.Therefore,the complex lipid profiles are responsible for the unique volatile flavors of flesh oil and liver oil,and the differential lipids play a central role in their volatile flavor formation.These findings provide a foundation for understanding the volatile flavor differences in fish oils and hold promise for further exploration of the molecular mechanisms underlying oil volatile flavors.
基金the National Natural Science Foundation of China(No.82374295)the National Key R&D Program of China(No.2021YFE0111100)+1 种基金the Science and Technol-ogy Partnership Program by the Ministry of Science and Technol-ogy of China(No.KY202201002)the Jiangsu Provincial De-partment of Science and Technology(No.BZ2022017).
文摘Cardiovascular disease(CVD)is a major global health challenge,which causes significant illness and death worldwide.These include a range of conditions that affect the heart and blood vessels,including coro-nary artery disease,stroke,peripheral artery disease,and heart failure.Despite advances in medicine and healthcare delivery,CVD continues to have a serious impact on individuals,families,and the healthcare system.This review begins by delineating the merits and demerits of commonly employed synthetic and natural materials for artificial blood vessels.It delves into various techniques commonly employed in the fabrication of artificial blood vessels,encompassing advanced textile technologies,electrospinning,ther-mally induced phase separation,and 3D printing.The review critically analyzes the attributes of different preparation methodologies alongside the latest advancements in research.The review also outlines the requisite performance requirements for artificial blood vessels,which encompass robust mechanical prop-erties,appropriate porosity,exceptional compatibility,and antibacterial attributes.It provides a succinct overview of ongoing effort s in vascular functionalization,particularly emphasizing thrombus mitigation,promotion of endothelialization,and enhancement of nitric oxide production.The review finally encap-sulates the primary challenges confronting vascular grafts and prospective avenues for future research.
基金supported by the National Natural Science Foundation of China(32101857 and U21A20218)the China Agricultural University Corresponding Support Research Joint Fund(GSAU-DKZY-2024-001)+1 种基金the Science and Technology Program in Gansu Province,China(24ZDNA008and23JRRA1407)the Fuxi Young Talents Fund of Gansu Agricultural University,China(Gaufx-03Y10).
文摘Intercropping has been widely used in arid and semi-arid regions because of its high yield,stable productivity,and efficient utilization of resources.However,in recent years,the high yield of traditional intercropping is mainly attributed to the large amount of purchased resources such as water and fertilizer,plastic film,and mechanical power.These lead to a decline in cultivated land quality and exacerbate intercrops'premature root and canopy senescence.So,the application of traditional intercropping faces major challenges in crop production.This paper analyzes the manifestations,occurrence mechanisms,and agronomic regulatory pathways of crop senescence.The physiological and ecological characteristics of intercropping to delay root and canopy senescence of crops are reviewed in this paper.The main agronomic regulatory pathways of intercropping to delay root and canopy senescence of crops are based on above-and blow-ground interactions,including collocation of crop varieties,spatial arrangement,water and fertilizer management,and tillage and mulch practices.Future research fields of intercropping to delay root and canopy senescence should focus on the aspects of selecting and breeding special varieties,application of molecular biology techniques,and developing or applying models to predict and evaluate the root and canopy senescence process of intercrops.Comprehensive analysis and evaluation of different research results could provide a basis for enhancing intercropping delay root and canopy senescence through adopting innovative technologies for regulating the physio-ecological characteristics of intercrops.This would support developing and adopting high-yield,efficient,and sustainable intercropping systems in arid and semi-arid areas with high population density,limited land,and abundant light and heat resources.
基金Supported by Science and Technology Development Plan Project of Weifang,No.2023YX005。
文摘BACKGROUND Cleidocranial dysplasia(CCD)is an infrequent clinical condition with an autosomal dominant inheritance pattern.It is characterized by abnormal clavicles,patent sutures and fontanelles,supernumerary teeth,and short stature.Approximately 60%-70%of patients with CCD have mutations in the RUNX family transcription factor 2 gene.However,prenatal diagnosis of CCD is difficult when the family history is unknown.CASE SUMMARY We report a rare case of fetal CCD with an unknown family history,confirmed by prenatal ultrasonography and genetic testing at a gestational age of 16 weeks.The genetic reports indicated that the fetus carried pathogenic mutations in the RUNX family transcription factor 2 gene(c.674G>A).After careful consideration,the pregnant woman and her family decided to continue the pregnancy.CONCLUSION Definitive prenatal diagnosis of CCD should include family history,ultrasound diagnosis,and genetic analysis,especially if family history is unknown.
基金supported by grants from the National Postdoctoral Science Foundation,China(2014M552219)the Natural Science Foundation of Guangdong Province,China(2015 A030313889,2015A030401013,2014A030313709,and 2014A030 313710)+1 种基金the Science and Technology Planning Project of Shenzhen Municipality,China(ZDSYS201504301045406,JCYJ20150403110 829621,JCYJ20150403091443301,JCYJ20140415162542975,JCYJ 20140415162338855,JCYJ20140828163634004,and JCYJ201206 16144352139)the Health and Family Planning Commission Project of Shenzhen Municipality,China(201401026)
文摘Abstract The dopamine transporter （DAT） is involved in the regulation of extracellular dopamine levels. A 40-bp variable-number tandem repeat （VNTR） polymorphism in the 3-untranslated region （3UTR） of the DAT has been reported to be associated with various phenotypes that are involved in the aberrant regulation of dopaminergic neu- rotransmission. In the present study, we found that miR- 137 and miR-491 caused a marked reduction of DAT expression, thereby influencing neuronal dopamine trans- port. Moreover, the regulation of miR-137 and miR-491 on this transport disappeared after the DAT was silenced. The miR-491 seed region that is located on the VNTR sequence in the 3＇UTR of the DAT and the regulatory effect of miR- 491 on the DAT depended on the VNTR copy-number. These data indicate that miR-137 and miR-491 regulate DAT expression and dopamine transport at the post- transcriptional level, suggesting that microRNA may be targeted for the treatment of diseases associated with DAT dysfunction.
基金partially supported by the National Key R&D Program of China(2018YFA0404602 and 2018YFA0404603)the National SKA Program of China(2020SKA0110300)+3 种基金the National Natural Science Foundation of China(NSFC,11963003,11763002,61572461,11790305,U1831204,U1931141,11961141001 and 11903009)the Guizhou Science&Technology Plan Project(Platform Talent No.[2017]5788,[2017]5781)the Youth Science&Technology Talents Development Project of Guizhou Education Department(No.KY[2018]119 and[2018]433)the Guizhou University Talent Research Fund(No.(2018)60)。
文摘A sky model from CLEAN deconvolution is a particularly effective high dynamic range reconstruction in radio astronomy,which can effectively model the sky and remove the sidelobes of the point spread function(PSF)caused by incomplete sampling in the spatial frequency domain.Compared to scale-free and multi-scale sky models,adaptive-scale sky modeling,which can model both compact and diffuse features,has been proven to have better sky modeling capabilities in narrowband simulated data,especially for large-scale features in high-sensitivity observations which are exactly one of the challenges of data processing for the Square Kilometre Array(SKA).However,adaptive scale CLEAN algorithms have not been verified by real observation data and allow negative components in the model.In this paper,we propose an adaptive scale model algorithm with non-negative constraint and wideband imaging capacities,and it is applied to simulated SKA data and real observation data from the Karl G.Jansky Very Large Array(JVLA),an SKA precursor.Experiments show that the new algorithm can reconstruct more physical models with rich details.This work is a step forward for future SKA image reconstruction and developing SKA imaging pipelines.
文摘BACKGROUND Gallbladder cancer is a highly malignant and aggressive tumor,often diagnosed at an advanced stage.The prognosis for advanced gallbladder cancer remains poor,with limited options for effective treatment.CASE SUMMARY A 65-year-old male patient presented with a soft tissue mass in the gallbladder.Following laparoscopic exploration,he underwent radical surgery for gallbladder cancer,with the tumor staged as pT2N1M0(stage III).Post-surgery,the patient received four cycles of adjuvant chemotherapy.However,one month later,over 60 metastatic lesions were detected in the liver,lungs,and lymph nodes.In response to the widespread metastasis,he underwent six cycles of combination therapy consisting of sintilimab,albumin-bound paclitaxel,and cisplatin.After two cycles,a partial response was achieved.Maintenance therapy was initiated with a combination of sintilimab and albumin-bound paclitaxel for four cycles.Monotherapy with sintilimab was continued thereafter until October 2023.Complete remission was confirmed in September 2021.The patient sustained this complete response for more than three years,including an eleven-month period without disease progression following the discontinuation of sintilimab.Genetic analysis revealed a tumor mutational burden of 15.4 mutations/megabase,which indicates a potentially favorable response to immunotherapy.CONCLUSION This case demonstrates the potential of combining immunotherapy(sintilimab)with chemotherapy to achieve durable remission in metastatic gallbladder cancer,even in a patient with extensive metastasis.In addition,it indicated the importance of tumor mutational burden as a predictive biomarker of immunotherapy.
文摘This study investigates how universities are developing normativeframeworks to regulate the use of generative AI tools in higher education, with aparticular focus on balancing empowerment and discipline. Drawing on theoreticallenses such as Foucault’s discipline theory and contemporary AI ethics, the paperanalyzes policy documents from institutions including Harvard, Oxford, and severaltop Chinese universities. Using Latent Dirichlet Allocation (LDA) topic modeling,the study reveals five dominant governance themes—ranging from academicintegrity enforcement to pedagogical empowerment. The findings highlight a globalshift from restrictive to balanced, ethics-informed AI governance, with significantdisciplinary variations. The paper concludes by proposing a “principledpermissiveness” model that combines transparency, accountability, andpedagogical innovation in future AI governance.
基金the financial support from the National Natural Science Foundation of China (No.42102127)the Postdoctoral Research Foundation of China (No.2024 M751860)。
文摘Cleat serves as the primary flow pathway for coalbed methane(CBM)and water.However,few studies consider the impact of local contact on two-phase flow within cleats.A visual generalized model of endogenous cleats was constructed based on microfluidics.A microscopic and mesoscopic observation technique was proposed to simultaneously capture gas-liquid interface morphology of pores and throat and the two-phase flow characteristics in entire cleat system.The local contact characteristics of cleats reduced absolute permeability,which resulted in a sharp increase in the starting pressure.The reduced gas flow capacity narrowed the co-infiltration area and decreased water saturation at the isotonic point in a hydrophilic environment.The increased local contact area of cleats weakened gas phase flow capacity and narrowed the co-infiltration area.Jumping events occurred in methane-water flow due to altered porosity caused by local contact in cleats.The distribution of residual phases changed the jumping direction on the micro-scale as well as the dominant channel on the mesoscale.Besides,jumping events caused additional energy dissipation,which was ignored in traditional two-phase flow models.This might contribute to the overestimation of relative permeability.The work provides new methods and insights for investigating unsaturated flow in complex porous media.
基金funded by the National Natural Science Foundation of China(Grant Nos.32272698,32441072,32122081)National Key Research and Development Program of China(Grant No.2023YFF1002000)+4 种基金Liaoning Province Youth Science Foundation A-Class Project(formerly Liaoning Natural Science Foundation Outstanding Youth Project,Grant No.2025-JQ-05)Liaoning Province’s Future Industry Frontier Technology Project(Grant Nos.2025JH2/101330184 and 2025JH2/101330185)National Postdoctoral Program for Innovative Talents(Grant No.BX20250016)Open Project Program of State Key Laboratory of Crop Stress Biology for Arid Areas of China(Grant No.SKLCSRHPKF2025017)HAAFS Science and Technology Innovation Special Project(Grant No.2023KJCXZX-JZS-10).
文摘Stomata are tiny pores on the plant leaf surface that regulate the exchange of water and gases between the plant and the external environment.They are crucial for photosynthesis,water use efficiency(WUE),and the plant’s ability to adapt to environmental changes.Stomatal movement is vital for understanding how plants adapt to environmental stress and optimize resource utilization.Changes in environmental conditions,especially the quality and intensity of light throughout the day,affect stomatal dynamics and diurnal behavior,which in turn impact photosynthetic efficiency and water-use efficiency.In this review,we summarize the biophysical principles and mechanisms of stomatal movement regulated by ion transport at the plasma membrane,vacuolar membrane and metabolic activity through persulfidation or S-nitrosylation modifications.Specifically,we focus on recent progress in the regulation of stomatal movement by different light qualities,and summarize the photochemical and biochemical events underlying photoreceptors as well as the knowledge of novel regulatory functions and signaling in the multilayer control of stomatal movement and environmental adaptation.Furthermore,as rising global temperatures and increased water needs of farming methods are expected to escalate future crop losses,we explore the potential of smart LED lighting and gene editing technology in enhancing photosynthetic efficiency and water-use efficiency,leading to increased crop biomass and higher crop yields.
基金supported by the Scientific and Technological Development Program of Jilin Province,China (Nos.20210203090SF and 20200403119SF)
文摘Inflammation represents a critical immune response triggered by cellular activities and inflammatory mediators following tissue damage.It plays a central role in the pathological progression of diverse diseases,including psychiatric disorders,cancer,and immunological conditions,rendering it an essential target for therapeutic intervention.Periodontitis,a prevalent oral inflammatory disease,is a leading cause of tooth loss and poses significant health challenges globally.Traditionally,inflammatory diseases such as periodontitis have been treated with systemic administration of synthetic chemicals.However,recent years have witnessed challenges,including drug resistance and microbial dysbiosis associated with these treatments.In contrast,natural products derived from Chinese medicine offer numerous benefits,such as high safety profiles,minimal side effects,innovative pharmacological mechanisms,ease of extraction,and multiple targets,rendering them viable alternatives to conventional antibiotics for treating inflammatory conditions.Numerous effective anti-inflammatory natural products have been identified in traditional Chinese medicine(TCM),including alkaloids,flavonoids,terpenoids,lignans,and other natural products that exhibit inhibitory effects on inflammation and are potential therapeutic agents.Several studies have confirmed the substantial anti-inflammatory and immunomodulatory properties of these compounds.This comprehensive review examines the literature on the anti-inflammatory effects of TCM-derived natural products from databases such as PubMed,Web of Science,and CNKI,focusing on terms like"inflammation","periodontitis","pharmacology",and"traditional Chinese medicine".The analysis systematically summarizes the molecular pharmacology,chemical composition,and biological activities of these compounds in inflammatory responses,alongside their mechanisms of action.This research seeks to deepen understanding of the mechanisms and biological activities of herbal extracts in managing inflammatory diseases,potentially leading to the development of promising new anti-inflammatory drug candidates.Future applications could extend to the treatment of various inflammatory conditions,including periodontitis.
基金Supported by the Qingdao Aquarium Technology Collaborative Innovation Center Cooperation Project(No.20210021)the Researching Key Technologies for Selecting Excellent Koi Carp Germplasm(No.20223702032291)the Qingdao Agricultural University Tangwang Koi Carp Joint R&D Center Collaborative Project(No.20220271)。
文摘FOXL 2 and CYP 19 B are crucial transcription factors in vertebrates and invertebrates that play pivotal roles in sex differentiation and gonadal development.The potential roles of the foxl 2 and cyp 19 b genes in sex determination and gonadal development in Cyprinus carpio var.koi were explored using a non-invasive RNA interference(RNAi)method,histopathological observation and qPCR.Results demonstrate that foxl 2 exhibited a sexually dimorphic expression pattern in gonads,with a notable expression in ovaries;cyp 19 b was expressed in all peripheral tissues,with a particularly prominent expression in brain and gonads.The knockdown of foxl 2 by RNAi resulted in delay in the development of the female gonads.Conversely,no notable alterations were discerned in the gonads of C.carpio var.koi following the knockdown of cyp 19 b.The upregulation of sox 9 a,amh,and cyp 19 b following foxl 2 knockdown indicates that foxl 2 may play a pivotal role in gonadal development.Nevertheless,further investigation is required to ascertain the potential role of cyp 19 b.This study elucidated the role of foxl 2 and enhanced the understanding of the mechanisms of sex determination and gonadal development in C.carpio var.koi.
