High-resolution video transmission requires a substantial amount of bandwidth.In this paper,we present a novel video processing methodology that innovatively integrates region of interest(ROI)identification and super-...High-resolution video transmission requires a substantial amount of bandwidth.In this paper,we present a novel video processing methodology that innovatively integrates region of interest(ROI)identification and super-resolution enhancement.Our method commences with the accurate detection of ROIs within video sequences,followed by the application of advanced super-resolution techniques to these areas,thereby preserving visual quality while economizing on data transmission.To validate and benchmark our approach,we have curated a new gaming dataset tailored to evaluate the effectiveness of ROI-based super-resolution in practical applications.The proposed model architecture leverages the transformer network framework,guided by a carefully designed multi-task loss function,which facilitates concurrent learning and execution of both ROI identification and resolution enhancement tasks.This unified deep learning model exhibits remarkable performance in achieving super-resolution on our custom dataset.The implications of this research extend to optimizing low-bitrate video streaming scenarios.By selectively enhancing the resolution of critical regions in videos,our solution enables high-quality video delivery under constrained bandwidth conditions.Empirical results demonstrate a 15%reduction in transmission bandwidth compared to traditional super-resolution based compression methods,without any perceivable decline in visual quality.This work thus contributes to the advancement of video compression and enhancement technologies,offering an effective strategy for improving digital media delivery efficiency and user experience,especially in bandwidth-limited environments.The innovative integration of ROI identification and super-resolution presents promising avenues for future research and development in adaptive and intelligent video communication systems.展开更多
A series of precious metals catalysts （M/TiO/, M = Ru, Rh, Pd, Ag, Ir, Pt or Au） were prepared by a light deposition method and the synergistic photocatalytic degradations of pyridine （20 mg/L） under UV irradiati...A series of precious metals catalysts （M/TiO/, M = Ru, Rh, Pd, Ag, Ir, Pt or Au） were prepared by a light deposition method and the synergistic photocatalytic degradations of pyridine （20 mg/L） under UV irradiation （365 nm） using M/TiO2 with electron capture agent KBrO3 have been investigated. The results show that KBrO3 has a greatly synergistic role on M/TiO2 and the photocatalytic activity of M/TiO2 is closely related to its work function. Ag could greatly enhance the activity of TiO2 due to the binding characteristics of pyridine on Ag. Under the conditions of 0.5 wt.% Ag loading, Ag/TiO2 concentration of 0.1 g/L, KlrO3 concentration of 10 mmol/L and reaction liquid pH value at 9, the pyridine can be degraded by 64% within 3 hr, doubled than TiO2 photocatalytic system. The degradation kinetics of pyridine follows first-order kinetics and k = 5.53 × 10-3 min^-1.展开更多
The photocatalytic degradation of phenol and 4-chlorophenol （4-CP） in aqueous suspensions with the use of titanium dioxide （TiO2 ） under UV irradiation was examined. The effects of different supporting materials m...The photocatalytic degradation of phenol and 4-chlorophenol （4-CP） in aqueous suspensions with the use of titanium dioxide （TiO2 ） under UV irradiation was examined. The effects of different supporting materials mixed physically with TiO2 were studied to achieve maximum degradation efficiency. Among the three supports, namely activated carbon （AC）, silica （SiO2 ） and zeolite （ZSM-5）, all exhibited paramount efficiency for degradation of phenol and 4-CP and was better than TiO2 alone. The optimum concentration was found to be 50 mg for all supporting materials. The efficiency order of the three supports was as follows： AC 〉 ZSM-5 〉 SiO2 , respectively. Whilst, the degradation of phenol and 4-CP was improved from 70.6% to 87.6% and 80.6% to 89.7%, respectively, within 120 min photocatalysis in the presence of optimal amount of AC. The degradation was also comparatively enhanced in the presence of cheaper rice husk and the activity was closed to ZSM-5 and lower than AC.展开更多
The solid acid catalyst, N-F codoped Ti O2/Si O2 composite oxide was prepared by a sol-gel method using NH4 F as nitrogen and fluorine source. The prepared materials were characterized by X-ray diffraction（XRD）, sca...The solid acid catalyst, N-F codoped Ti O2/Si O2 composite oxide was prepared by a sol-gel method using NH4 F as nitrogen and fluorine source. The prepared materials were characterized by X-ray diffraction（XRD）, scanning electron microscopy（SEM）, X-ray photoelectron spectroscopy（XPS）, UV-Visible diffuse reflectance spectroscopy（UV-Vis）,ammonia adsorption and temperature-programmed desorption（NH3-TPD）, in situ Fourier transform infrared spectroscopy（FT-IR） and N2 physical adsorption isotherm. The photocatalytic activity of the catalyst for acrylonitrile degradation was investigated under simulant solar irradiation. The results showed that strong Lewis and Brnsted acid sites appear on the surface of the sample after N-F doping. Systematic investigation showed that the highest photocatalytic activity for acrylonitrile degradation was obtained for samples calcined at 450°C with molar ratio（NH4F to Ti） of 0.8. The degradation ratio of 71.5% was achieved with the prepared catalyst after 6-min irradiation, demonstrating the effectiveness of photocatalytic degradation of acrylonitrile with N-F codoped Ti O2/Si O2 composite oxide. The photocatalyst is promising for application under solar light irradiation.Moreover, the intermediates generated after irradiation were verified by gas chromatography-mass spectrometry（GC-MS） analysis and UV-Vis spectroscopy to be simple organic acids with lower toxicity, and the degradation pathway was also proposed for acrylonitrile degradation with the prepared catalyst.展开更多
BACKGROUND： Overexpression of α-synuclein can induce cell apoptosis. RNA interference （RNAi） may block specific gene function and cause gene silencing. OBJECTIVE： To construct a specific and effective RNAi plasmi...BACKGROUND： Overexpression of α-synuclein can induce cell apoptosis. RNA interference （RNAi） may block specific gene function and cause gene silencing. OBJECTIVE： To construct a specific and effective RNAi plasmid for the α-synuclein gene and investigate if RNAi can block apoptosis in HEK293 cells, induced by overexpression of wild-type α-synuclein. DESIGN, TIME AND SETTING： A contrast experiment based on genetically engineered cytobiology was performed at the State Key Lab of Medical Genetics of China, Xiangya Medical College of Central South University, between October 2004 and October 2008. MATERIALS： HEK293 cells and pBSHH1 plasmid were provided by the State Key Lab of Medical Genetics of China; OligDNA sequence by Sagon Bioengineering Company, Shanghai; Lipofectamine 2000 by Invitrogen, USA; α-synuclein monoclonal antibody, Hoechst 33258, and MTT by Sigma, USA; Horseradish peroxidase-coupled goat anti-rat IgG by KPL, USA; FACSan flow cytometry by BD, USA. METHODS： Four target sites were used to construct hairpin RNA pBSHH1 vectors - pSYNi-1, pSYNi-2, pSYNi-3 and pSYNi-4 - which were cloned in the pBSHH1 plasmid. HEK293 cells were transfected using Lipofectamine 2000. In addition, a non-transfect group and a negative plasmid transfect group were established. The cultured HEK293 cells were processed as follows： transfection of blank plasmid （blank control group）, transfection of α-synuclein-pEGFP and RNAi negative vector （negative control group）, and transfection of α-synuclein-pEGFP and pSYNi-1 （transfection group）. Cells in all groups were transfected with Lipofectamine 2000 for 48 hours. MAIN OUTCOME MEASURES： Expression of α-synuclein mRNA and protein were detected by RT-PCR and Western blot. Cell morphology was observed under an inverted fluorescence microscope; cell viability was measured using MTT method; and cell apoptosis was determined with Annexin V-PE flow cytometry. RESULTS： α-synuclein mRNA and protein expressions were significantly decreased in the pSYNi-1 group when compared with the non-transfect and negative plasmid transfect groups （P 〈 0.05）. The expressions were partially decreased in the pSYNi-2 group, but there was no significant difference in the pSYNi-3 and pSYNi-4 groups. Hoechst staining indicated that cell nuclei were enlarged in the negative control group, coloring was not uniform, and chromatin was accumulated and appeared spot-like. The nucleus coloring was uniform in the transfection group compared to negative control group. Cell viability in the negative control group was significantly lower than blank control group with cell apoptosis being significantly increased （P 〈 0.05）. In comparison with negative control group, cell viability was significantly increased in the transfection group and cell apoptosis was significantly decreased （P 〈 0.05）. CONCLUSION： pSYNi-1 can inhibit α-synuclein gene expression and block apoptosis of HEK293 cells induced by overexpression of wild-type α-synuclein.展开更多
[Objectives] To establish a method for the determination of gallic acid in Shuijia Zhixue capsule and Rotala rotundifolia .[Methods] High performance liquid chromatography (HPLC) and Phenomenex C 18 column were used, ...[Objectives] To establish a method for the determination of gallic acid in Shuijia Zhixue capsule and Rotala rotundifolia .[Methods] High performance liquid chromatography (HPLC) and Phenomenex C 18 column were used, the mobile phase was 0.1% phosphoric acid∶ methanol=85∶ 15, the flow rate was 0.5 mL/min, and the detection wavelength was 270 nm.[Results] In the range of (2-25)μg/mL , the concentration of gallic acid had a good linear relationship with the peak area, r =0.990 8;the average recovery was 96.88%.[Conclusions] The method was simple, sensitive and reproducible. It provided a basis for the analysis of gallic acid in Rotala rotundifolia and Shuijia Zhixue capsule and the establishment of quality detection methods in the future.展开更多
This study proved the significance of simulated sunlight irradiation response capability of Sn-F co-doped TiO_(2)/SiO_(2)(Sn-F-TiO_(2)/SiO_(2))photocatalysts,which were prepared by a simple sol-gel method and were eva...This study proved the significance of simulated sunlight irradiation response capability of Sn-F co-doped TiO_(2)/SiO_(2)(Sn-F-TiO_(2)/SiO_(2))photocatalysts,which were prepared by a simple sol-gel method and were evaluated by acrylonitrile degradation for photocatalytic activity.The synthesized catalysts were characterized by X-ray Diffraction(XRD),Scanning Electron Microscopy(SEM),Energy Dispersive Spectrometer(EDS),X-ray Photoelectron Spectroscopy(XPS),Brunauer-Emmett-Teller(BET),Ultraviolet-Visible Absorption spectroscopy(UV-Vis),and Photoluminescence Spectroscopy(PL).UV-Visible spectroscopy demonstrated that Sn doping caused remarkable red shift in TiO_(2),which significantly increased the absorption efficiency of the catalysts.The XPS results showed that Sn was successfully doped into the TiO_(2) lattice.The photocatalytic degradation of acrylonitrile indicated that the Sn-F-TiO_(2)/SiO_(2) photocatalysts exhibited excellent photocatalytic activity when being annealed at 550℃for 2 h.The degradation rate of acrylonitrile reached 67.7%after irradiation under simulated sunlight for 6 min,and the hole was the most important active species.展开更多
This paper reviews the studies of using FTIR to investigate the components of aerosols produced in smog chamber experiments and collected in atmosphere. The fact that aerosols are mixture of small amount of countless ...This paper reviews the studies of using FTIR to investigate the components of aerosols produced in smog chamber experiments and collected in atmosphere. The fact that aerosols are mixture of small amount of countless individual compounds makes the analysis of aerosol constituents very challenging. Although a number of advanced instruments have been applied to the chemical characterization of aerosol components, the majority of aerosol components, particularly the organics, remain unknown. Being supplemental to the traditional quantitative instruments, FTIR has been recently used either individually or combining with other analytical instruments to characterize the components of aerosol particles. This paper aims to show how FTIR is applied to analysis of organic aerosols in current literature and to summarize the FTIR characteristic peak frequencies that are widely seen in the FTIR measurement of organic aerosols. It will be greatly helpful to researchers whose studies are focused on the analysis of aerosol components.展开更多
Abdominal aortic aneurysm(AAA)is strongly correlated with obesity,partially due to the abnormal expansion of abdominal perivascular adipose tissue(PVAT).Cell death-inducing DNA fragmentation factor-like effector C(CID...Abdominal aortic aneurysm(AAA)is strongly correlated with obesity,partially due to the abnormal expansion of abdominal perivascular adipose tissue(PVAT).Cell death-inducing DNA fragmentation factor-like effector C(CIDEC),also known as fat-specific protein 27(FSP27)in rodents,is specifically expressed in adipose tissue where it mediates lipid droplet fusion and adipose tissue expansion.Whether and how CIDEC/FSP27 plays a role in AAA pathology remains elusive.Here,we show that FSP27 exacerbates obesity and angiotensinⅡ(AngⅡ)-induced AAA progression.FSP27 deficiency in mice inhibited high-fat diet-induced PVAT expansion and inflammation.Both global and adipose tissue-specific FSP27 ablation significantly decreased obesity-related AAA incidence.Deficiency of FSP27 in adipocytes abrogated matrix metalloproteinase-12(MMP12)expression in aortic tissues.Infiltrated macrophages,which partially colocalize with MMP12,were significantly decreased in the FSP27-deficient aorta.Mechanistically,knockdown of Fsp27 in 3T3-L1 adipocytes inhibited C-C motif chemokine ligand 2(CCL2)expression and secretion through a c-Jun N-terminal kinase(JNK)-dependent pathway,thereby leading to reduced induction of macrophage migration,while Cidec overexpression rescued this effect.Overall,our study demonstrates that CIDEC/FSP27 in adipose tissue contributes to obesity-related AAA formation,at least in part,by enhancing PVAT inflammation and macrophage infiltration,thus shedding light on its significance as a key regulator in the context of obesity-related AAA.展开更多
基金funded by National Key Research and Development Program of China(No.2022YFC3302103).
文摘High-resolution video transmission requires a substantial amount of bandwidth.In this paper,we present a novel video processing methodology that innovatively integrates region of interest(ROI)identification and super-resolution enhancement.Our method commences with the accurate detection of ROIs within video sequences,followed by the application of advanced super-resolution techniques to these areas,thereby preserving visual quality while economizing on data transmission.To validate and benchmark our approach,we have curated a new gaming dataset tailored to evaluate the effectiveness of ROI-based super-resolution in practical applications.The proposed model architecture leverages the transformer network framework,guided by a carefully designed multi-task loss function,which facilitates concurrent learning and execution of both ROI identification and resolution enhancement tasks.This unified deep learning model exhibits remarkable performance in achieving super-resolution on our custom dataset.The implications of this research extend to optimizing low-bitrate video streaming scenarios.By selectively enhancing the resolution of critical regions in videos,our solution enables high-quality video delivery under constrained bandwidth conditions.Empirical results demonstrate a 15%reduction in transmission bandwidth compared to traditional super-resolution based compression methods,without any perceivable decline in visual quality.This work thus contributes to the advancement of video compression and enhancement technologies,offering an effective strategy for improving digital media delivery efficiency and user experience,especially in bandwidth-limited environments.The innovative integration of ROI identification and super-resolution presents promising avenues for future research and development in adaptive and intelligent video communication systems.
基金supported by the National Natural Science Foundation of China(No.20907012)the National High Technology Research and Development Program of China(No.2012ZX07206-002)the Special Fund for the Development of Strategic and New Industry in Shenzhen(No.JCYJ20120613114951217)
文摘A series of precious metals catalysts （M/TiO/, M = Ru, Rh, Pd, Ag, Ir, Pt or Au） were prepared by a light deposition method and the synergistic photocatalytic degradations of pyridine （20 mg/L） under UV irradiation （365 nm） using M/TiO2 with electron capture agent KBrO3 have been investigated. The results show that KBrO3 has a greatly synergistic role on M/TiO2 and the photocatalytic activity of M/TiO2 is closely related to its work function. Ag could greatly enhance the activity of TiO2 due to the binding characteristics of pyridine on Ag. Under the conditions of 0.5 wt.% Ag loading, Ag/TiO2 concentration of 0.1 g/L, KlrO3 concentration of 10 mmol/L and reaction liquid pH value at 9, the pyridine can be degraded by 64% within 3 hr, doubled than TiO2 photocatalytic system. The degradation kinetics of pyridine follows first-order kinetics and k = 5.53 × 10-3 min^-1.
文摘The photocatalytic degradation of phenol and 4-chlorophenol （4-CP） in aqueous suspensions with the use of titanium dioxide （TiO2 ） under UV irradiation was examined. The effects of different supporting materials mixed physically with TiO2 were studied to achieve maximum degradation efficiency. Among the three supports, namely activated carbon （AC）, silica （SiO2 ） and zeolite （ZSM-5）, all exhibited paramount efficiency for degradation of phenol and 4-CP and was better than TiO2 alone. The optimum concentration was found to be 50 mg for all supporting materials. The efficiency order of the three supports was as follows： AC 〉 ZSM-5 〉 SiO2 , respectively. Whilst, the degradation of phenol and 4-CP was improved from 70.6% to 87.6% and 80.6% to 89.7%, respectively, within 120 min photocatalysis in the presence of optimal amount of AC. The degradation was also comparatively enhanced in the presence of cheaper rice husk and the activity was closed to ZSM-5 and lower than AC.
基金financially supported by the Science and Technology Innovation Commission of Shenzhen Municipality, China (No. JCYJ20120613154128107)
文摘The solid acid catalyst, N-F codoped Ti O2/Si O2 composite oxide was prepared by a sol-gel method using NH4 F as nitrogen and fluorine source. The prepared materials were characterized by X-ray diffraction（XRD）, scanning electron microscopy（SEM）, X-ray photoelectron spectroscopy（XPS）, UV-Visible diffuse reflectance spectroscopy（UV-Vis）,ammonia adsorption and temperature-programmed desorption（NH3-TPD）, in situ Fourier transform infrared spectroscopy（FT-IR） and N2 physical adsorption isotherm. The photocatalytic activity of the catalyst for acrylonitrile degradation was investigated under simulant solar irradiation. The results showed that strong Lewis and Brnsted acid sites appear on the surface of the sample after N-F doping. Systematic investigation showed that the highest photocatalytic activity for acrylonitrile degradation was obtained for samples calcined at 450°C with molar ratio（NH4F to Ti） of 0.8. The degradation ratio of 71.5% was achieved with the prepared catalyst after 6-min irradiation, demonstrating the effectiveness of photocatalytic degradation of acrylonitrile with N-F codoped Ti O2/Si O2 composite oxide. The photocatalyst is promising for application under solar light irradiation.Moreover, the intermediates generated after irradiation were verified by gas chromatography-mass spectrometry（GC-MS） analysis and UV-Vis spectroscopy to be simple organic acids with lower toxicity, and the degradation pathway was also proposed for acrylonitrile degradation with the prepared catalyst.
基金the National "863" High-Technology Research and Development Program Grant,No. 2004AA2270402002BA711A07+3 种基金the National "Tenth-Five Year" Science and Technology Program,No. 2004BA720A03the National Natural Science Foundation of China,No. 30370515the Natural Science Foundation of Hainan Province,No. 806119807080
文摘BACKGROUND： Overexpression of α-synuclein can induce cell apoptosis. RNA interference （RNAi） may block specific gene function and cause gene silencing. OBJECTIVE： To construct a specific and effective RNAi plasmid for the α-synuclein gene and investigate if RNAi can block apoptosis in HEK293 cells, induced by overexpression of wild-type α-synuclein. DESIGN, TIME AND SETTING： A contrast experiment based on genetically engineered cytobiology was performed at the State Key Lab of Medical Genetics of China, Xiangya Medical College of Central South University, between October 2004 and October 2008. MATERIALS： HEK293 cells and pBSHH1 plasmid were provided by the State Key Lab of Medical Genetics of China; OligDNA sequence by Sagon Bioengineering Company, Shanghai; Lipofectamine 2000 by Invitrogen, USA; α-synuclein monoclonal antibody, Hoechst 33258, and MTT by Sigma, USA; Horseradish peroxidase-coupled goat anti-rat IgG by KPL, USA; FACSan flow cytometry by BD, USA. METHODS： Four target sites were used to construct hairpin RNA pBSHH1 vectors - pSYNi-1, pSYNi-2, pSYNi-3 and pSYNi-4 - which were cloned in the pBSHH1 plasmid. HEK293 cells were transfected using Lipofectamine 2000. In addition, a non-transfect group and a negative plasmid transfect group were established. The cultured HEK293 cells were processed as follows： transfection of blank plasmid （blank control group）, transfection of α-synuclein-pEGFP and RNAi negative vector （negative control group）, and transfection of α-synuclein-pEGFP and pSYNi-1 （transfection group）. Cells in all groups were transfected with Lipofectamine 2000 for 48 hours. MAIN OUTCOME MEASURES： Expression of α-synuclein mRNA and protein were detected by RT-PCR and Western blot. Cell morphology was observed under an inverted fluorescence microscope; cell viability was measured using MTT method; and cell apoptosis was determined with Annexin V-PE flow cytometry. RESULTS： α-synuclein mRNA and protein expressions were significantly decreased in the pSYNi-1 group when compared with the non-transfect and negative plasmid transfect groups （P 〈 0.05）. The expressions were partially decreased in the pSYNi-2 group, but there was no significant difference in the pSYNi-3 and pSYNi-4 groups. Hoechst staining indicated that cell nuclei were enlarged in the negative control group, coloring was not uniform, and chromatin was accumulated and appeared spot-like. The nucleus coloring was uniform in the transfection group compared to negative control group. Cell viability in the negative control group was significantly lower than blank control group with cell apoptosis being significantly increased （P 〈 0.05）. In comparison with negative control group, cell viability was significantly increased in the transfection group and cell apoptosis was significantly decreased （P 〈 0.05）. CONCLUSION： pSYNi-1 can inhibit α-synuclein gene expression and block apoptosis of HEK293 cells induced by overexpression of wild-type α-synuclein.
基金Supported by the Project of Sichuan Provincial Department of Science and Technology(2017SZYZF0012)Research Program Foundation of Science and Technology Bureau of Liangshan Yi Autonomous Prefecture(15yyjs0002)
文摘[Objectives] To establish a method for the determination of gallic acid in Shuijia Zhixue capsule and Rotala rotundifolia .[Methods] High performance liquid chromatography (HPLC) and Phenomenex C 18 column were used, the mobile phase was 0.1% phosphoric acid∶ methanol=85∶ 15, the flow rate was 0.5 mL/min, and the detection wavelength was 270 nm.[Results] In the range of (2-25)μg/mL , the concentration of gallic acid had a good linear relationship with the peak area, r =0.990 8;the average recovery was 96.88%.[Conclusions] The method was simple, sensitive and reproducible. It provided a basis for the analysis of gallic acid in Rotala rotundifolia and Shuijia Zhixue capsule and the establishment of quality detection methods in the future.
基金Sponsored by the Science and Technology Commission of Shenzhen Municipality,P.R.China(Grant Nos.JCYJ20140417172417138 and ZDSYS20140508161622508).
文摘This study proved the significance of simulated sunlight irradiation response capability of Sn-F co-doped TiO_(2)/SiO_(2)(Sn-F-TiO_(2)/SiO_(2))photocatalysts,which were prepared by a simple sol-gel method and were evaluated by acrylonitrile degradation for photocatalytic activity.The synthesized catalysts were characterized by X-ray Diffraction(XRD),Scanning Electron Microscopy(SEM),Energy Dispersive Spectrometer(EDS),X-ray Photoelectron Spectroscopy(XPS),Brunauer-Emmett-Teller(BET),Ultraviolet-Visible Absorption spectroscopy(UV-Vis),and Photoluminescence Spectroscopy(PL).UV-Visible spectroscopy demonstrated that Sn doping caused remarkable red shift in TiO_(2),which significantly increased the absorption efficiency of the catalysts.The XPS results showed that Sn was successfully doped into the TiO_(2) lattice.The photocatalytic degradation of acrylonitrile indicated that the Sn-F-TiO_(2)/SiO_(2) photocatalysts exhibited excellent photocatalytic activity when being annealed at 550℃for 2 h.The degradation rate of acrylonitrile reached 67.7%after irradiation under simulated sunlight for 6 min,and the hole was the most important active species.
文摘This paper reviews the studies of using FTIR to investigate the components of aerosols produced in smog chamber experiments and collected in atmosphere. The fact that aerosols are mixture of small amount of countless individual compounds makes the analysis of aerosol constituents very challenging. Although a number of advanced instruments have been applied to the chemical characterization of aerosol components, the majority of aerosol components, particularly the organics, remain unknown. Being supplemental to the traditional quantitative instruments, FTIR has been recently used either individually or combining with other analytical instruments to characterize the components of aerosol particles. This paper aims to show how FTIR is applied to analysis of organic aerosols in current literature and to summarize the FTIR characteristic peak frequencies that are widely seen in the FTIR measurement of organic aerosols. It will be greatly helpful to researchers whose studies are focused on the analysis of aerosol components.
基金supported by the National Natural Science Foundation of China(32271334,32100945,and 81871228)the National Key R&D Program of China(2018YFA0506900 and 2018YFA0800301)+3 种基金Shanghai Basic Research Field Project“Science and Technology Innovation Action Plan”(21JC1400400)the Lingang Laboratory(LG-QS-202204-06)the High-Level Medicine Foundation of Shanghai Government(to P.L.)Shanghai Municipal Science and Technology Major Project(2017SHZDZX01).
文摘Abdominal aortic aneurysm(AAA)is strongly correlated with obesity,partially due to the abnormal expansion of abdominal perivascular adipose tissue(PVAT).Cell death-inducing DNA fragmentation factor-like effector C(CIDEC),also known as fat-specific protein 27(FSP27)in rodents,is specifically expressed in adipose tissue where it mediates lipid droplet fusion and adipose tissue expansion.Whether and how CIDEC/FSP27 plays a role in AAA pathology remains elusive.Here,we show that FSP27 exacerbates obesity and angiotensinⅡ(AngⅡ)-induced AAA progression.FSP27 deficiency in mice inhibited high-fat diet-induced PVAT expansion and inflammation.Both global and adipose tissue-specific FSP27 ablation significantly decreased obesity-related AAA incidence.Deficiency of FSP27 in adipocytes abrogated matrix metalloproteinase-12(MMP12)expression in aortic tissues.Infiltrated macrophages,which partially colocalize with MMP12,were significantly decreased in the FSP27-deficient aorta.Mechanistically,knockdown of Fsp27 in 3T3-L1 adipocytes inhibited C-C motif chemokine ligand 2(CCL2)expression and secretion through a c-Jun N-terminal kinase(JNK)-dependent pathway,thereby leading to reduced induction of macrophage migration,while Cidec overexpression rescued this effect.Overall,our study demonstrates that CIDEC/FSP27 in adipose tissue contributes to obesity-related AAA formation,at least in part,by enhancing PVAT inflammation and macrophage infiltration,thus shedding light on its significance as a key regulator in the context of obesity-related AAA.
