BACKGROUND Sleeve fracture of the patella is an unusual fracture,almost unique to children.The majority of sleeve fractures involve the inferior patellar pole.However,sleeve fractures of the superior pole of the patel...BACKGROUND Sleeve fracture of the patella is an unusual fracture,almost unique to children.The majority of sleeve fractures involve the inferior patellar pole.However,sleeve fractures of the superior pole of the patella are extremely rare in adults.CASE SUMMARY An 18-year-old male patient fell while running in the morning.The patient had tenderness to palpation at the superior pole of the patella,with a palpable gap over the upper part of the patella in both knees.We applied two 4.5-mm suture anchors with the Krackow stitch to repair the sleeve fracture,augmented by autogenous gracilis through performing the figure-of-eight technique.The patient regained approximately the full range of motion of the knee joint without any quadriceps weakness and a normal gait 6 weeks after surgery.CONCLUSION Sleeve fractures of the superior pole of the patella are extremely rare in adults,especially bilateral sleeve fractures.Suture anchors,augmented by autogenous gracilis,provided secure fixation and achieved excellent results in this rare injury.展开更多
Enhancing the lubricating properties and antibacterial adhesion resistance of implantable medical materials is critical to prevent soft tissue injury during implantation and the formation of bacterial biofilms.Prior s...Enhancing the lubricating properties and antibacterial adhesion resistance of implantable medical materials is critical to prevent soft tissue injury during implantation and the formation of bacterial biofilms.Prior studies may have exhibited limitations in the preparation methodologies and long-term stability of coatings for implantable medical materials.In this study,we developed a multilayered hybrid hydrogel coating method based on the rate difference of polymerization initiation on the material surface.The acquired coating with persistent lubrication capability retained its functionality after 2×10^(4) cycles of friction and 21 days of PBS immersion.A quaternary ammonium salt coating with antibacterial properties was introduced to further functionalize the coating.Animal experiments demonstrated that this coating exhibited remarkable effects on delaying encrustation and bacterial colonization.These studies indicate that this simple method of introducing lubricating and antibacterial coatings on catheters is likely to enhance the biocompatibility of medical devices and has broad application prospects in this field of medical devices.展开更多
Objective： To explore the effects and mechanism of glycogen synthase kinase 3β （GSK-313） inhibitor （2＇Z,3＇E）-6-bromo-indirubin-3＇-oxime （BIO） on drug resistance in colon cancer cells. Methods： The colon c...Objective： To explore the effects and mechanism of glycogen synthase kinase 3β （GSK-313） inhibitor （2＇Z,3＇E）-6-bromo-indirubin-3＇-oxime （BIO） on drug resistance in colon cancer cells. Methods： The colon cancer SW480 and SW620 cells were treated with BIO, 5-fluorouracil （5-FU） and BIO/5-FU, separately. Cell cycle distribution, apoptosis level and efflux ability of rhodamine 123 （Rh123） were detected by flow cytometry. The protein expressions of P-glycoprotein （P-gp）, multidrug resistance protein 2 （MRP2）, thymidylate synthase （TS）, β-catenin, E2F-1 and βcl-2 were detected by Western blot. β-catenin and P-gp were stained with double immunofluorescence and observed under a confocal microscope. Results： BIO up-regulated β-catenin, P-gp, MRP2 and TS, enhanced the efflux ability of Rh123, decreased Bcl-2 protein and gave the opposite effect to E2F-1 protein in SW480 and SW620 ceils. Furthermore, BIO significantly inhibited cell apoptosis, increased S and G2/M phase cells, and reduced the cell apoptosis induced by 5-FU in SW480 cells, whereas the effects were slight or not obvious in SW620 cells. Conclusion： GSK-3β was involved in drug resistance regulation, and activation of β-catenin and inhibition of E2F-1 may be the most responsible for the enhancement of 5-FU chemotherapy resistance induced by GSK-β inhibitor β10 in colon cancer.展开更多
Fluorinated diols(FDO)with dangling chain were introduced into polyether urethanes(PEUs)as chain extender in order to achieve internal plasticization.Based on temperature dependent Fourier transform infrared(FTIR)spec...Fluorinated diols(FDO)with dangling chain were introduced into polyether urethanes(PEUs)as chain extender in order to achieve internal plasticization.Based on temperature dependent Fourier transform infrared(FTIR)spectroscopy results,the fluorinated polyether urethanes(FPEUs)exhibited weaker hydrogen bonding between C=O and N―H groups with addition of FDO.The crystallinity of hard domain was destroyed determined by thermal analysis.Combined with the results of dynamic mechanical analysis(DMA)and dissipative particle dynamics(DPD)simulation,it was proved that more loosely aggregated hard domains with weakened interaction separated from soft segment were formed.Rheological analysis indicated that FDO reduced the complex viscosity and viscous flow activation energy which endowed the polymer with more flexibility.Despite the loss of hydrogen bonds,there was no significant decline in mechanical property resulting from the decrease of hard segment dissolved in soft segment phase.The presence of fluorinated carbon side chain could function as internal plasticizer and contribute to the processability of polyurethane.展开更多
Manipulating magnetic domain structure plays a key role in advanced spintronics devices.Theoretical rationale is that the labyrinthine domain structure,normally appearing in ferromagnetic thin films with strong magnet...Manipulating magnetic domain structure plays a key role in advanced spintronics devices.Theoretical rationale is that the labyrinthine domain structure,normally appearing in ferromagnetic thin films with strong magnetic anisotropy,shows a great potential to increase data storage density for designing magnetic nonvolatile memory and logic devices.However,an electrical control of labyrinthine domain structure remains elusive.Here,we demonstrate the gate-driven evolution of labyrinthine domain structures in an itinerant ferromagnet Cr_(7)Te_(8).By combining electric transport measurements and micromagnetic finite difference simulations,we find that the hysteresis loop of anomalous Hall effect in Cr_(7)Te_(8)samples shows distinct features corresponding to the generation of labyrinthine domain structures.The labyrinthine domain structures are found to be electrically tunable via Li-electrolyte gating,and such gate-driven evolution in Cr_(7)Te_(8)originates from the reduction of the magnetic anisotropic energy with gating,revealed by our micromagnetic simulations.Our results on the gate control of anomalous Hall effect in an itinerant magnetic material provide an opportunity to understand the formation and evolution of labyrinthine domain structures,paving a new route towards electric-field driven spintronics.展开更多
With the increasing incidence of breast cancer globally,there is an urgent demand for enhanced diagnostic and therapeutic strategies.This comprehensive review explores advancements in fluorescent probe technology for ...With the increasing incidence of breast cancer globally,there is an urgent demand for enhanced diagnostic and therapeutic strategies.This comprehensive review explores advancements in fluorescent probe technology for breast cancer imaging and early diagnosis.Fluorescent probes offer unparalleled sensitivity and specificity in detecting cancer biomarkers,enabling early diagnosis and delineation of tumor margins during surgery.We categorize these probes into molecular,nano,ratiometric,the second near-infrared window(NIR-II),and multifunctional varieties,each with unique capabilities for targeting specific cancer markers.This review highlights recent innovations in probe design,emphasizing their applications in pathogenesis studies,drug development,precision medicine,and fluorescence-guided surgery.Despite their promising clinical potential,challenges such as safety,biomarker targeting accuracy,system compatibility,and clinical translation persist.Addressing these hurdles is crucial for the integration of fluorescent probes into standard cancer care,aiming to improve treatment outcomes and patient quality of life.展开更多
Hepatocellular carcinoma （HCC） is one of the most frequent malignant tumors and is the second most common cause of cancer death in China. Therefore, it is very important to detect this disease and the recurrence at ...Hepatocellular carcinoma （HCC） is one of the most frequent malignant tumors and is the second most common cause of cancer death in China. Therefore, it is very important to detect this disease and the recurrence at its earlier period. Serum tumor markers, as the effective method for detecting hepatocellular carcinoma for a long time, could be divided into 4 categories： oncofetal antigens and glycoprotein antigens; enzymes and isoenzymes; genes; and cytokines. Serum alpha fetoprotein （AFP） is the most widely used tumor marker in detecting patients with hepatocellular carcinoma, and has been proven to have capability of prefiguring the prognosis. However, it has been indicated that AFP-L3 and DCP excel AFP in differentiating hepatocellular carcinoma from nonmalignant hepatopathy and detecting small hepatocellular carcinoma. Some tumor markers, such as human cervical cancer oncogene and human telomerase reverse transcriptase mRNA, have also been indicated to have higher accuracies than AFP. Furthermore, some other tumor markers, such as glypican-3, gamma-glutamyl transferase Ⅱ, alpha-Ifucosidase, transforming growth factor-beta1, tumorspecific growth factor, have been indicated to be available supplementaries to AFP in the detection. AFP mRNA has been shown to correlate with the metastasis and recurrence of HCC, and it may be the most useful marker to prefigure the prognosis. Some other markers, such as gamma-glutamyl transferase mRNA, vascular endothelial growth factor, and interleukin-8, could also be used as available prognostic indicators, and the simultaneous determination of AFP and these markers may detect the recurrence of HCC at its earlier period.展开更多
Differences between the Cenozoic and pre-Cenozoic strata and structures in the middlesouth South Yellow Sea are analyzed using high-resolution airborne gravity data combined with data from offshore wells, seismic expl...Differences between the Cenozoic and pre-Cenozoic strata and structures in the middlesouth South Yellow Sea are analyzed using high-resolution airborne gravity data combined with data from offshore wells, seismic exploration and the regional geological background, using forward and inverse methods. We discuss why hydrocarbon exploration has so far failed in the South Yellow Sea, and put forward alternative future exploration plans. The results show that there are thick Cenozoic strata over Jurassic-Cretaceous continental strata in the southern basin of the South Yellow Sea, contrasted with thick Mid-Paleozoic marine strata preserved in the middle uplift area. In the mid-southern South Yellow Sea, airborne Bouguer gravity anomalies are fragmentized with scattered local anomalies. Many tensile normal faults and minor fault blocks occur in the Cenozoic strata. In contrast, reverse faults and nappe structures are found in pre-Cenozoic strata. The essential reasons for the lack of a breakthrough in hydrocarbon exploration are the complexity of geological structures, hydrocarbon accumulation environments, seismic-geologic conditions, and the difficulty of interpreting and understanding these features, rather than an imbalance of exploration framework. Hydrocarbon exploration should be targeted at Mid-Cenozoic continental strata, especially of Paleogene age, in the middle to southern parts of the South Yellow Sea. Special attention should be paid to the favorable structural belts SYI1, SYI2, SYI3 and SYI4 in the southern basin of the South Yellow Sea, where detailed seismic exploration or drilling should be carried out. Attention should also be given to Mid-Paleozoic marine strata in the middle uplift area of the South Yellow Sea. Key strategies that could lead to a hydrocarbon exploration breakthrough are improving exploration resolution, conducting detailed studies of fine structures, and accurately locating minor structures.展开更多
The underwater topography in the offshore sea area of north Jiangsu is complicated, including the middle radial sand ridges and northern Haizhou bay underwater shoal. Therefore, it forms special marine dynamic conditi...The underwater topography in the offshore sea area of north Jiangsu is complicated, including the middle radial sand ridges and northern Haizhou bay underwater shoal. Therefore, it forms special marine dynamic conditions and typical shoal wetland ecosystem. Previous researches of these sea areas were mainly focused on the forms of morphogenesis and the characteristic of conformation of sand ridges. Few studies have done on three dimensional hydrodynamic and water quality simulation. This paper introduced EFDC (Environ-mental Fluid Dynamics Code) to study the tidal current fields, dilution and diffusion of the sewage outlet near Yangkou Port. Comparison between computation results and the observed data indicates that this model could reasonably simulate hydrodynamic fields. Based on the computed tidal current field, the distributions of COD concentration were simulated. The range of contamination diffusion derived from sewage outlet was very limited, and the influence range of sewage came to the maximum when ebb slacks in neap tide period.展开更多
Postharvest broccoli is prone to yellowing during storage,which is the key factor leading to a reduction in value.To explore appropriate control methods,it is important to understand the mechanisms of yellowing.We ana...Postharvest broccoli is prone to yellowing during storage,which is the key factor leading to a reduction in value.To explore appropriate control methods,it is important to understand the mechanisms of yellowing.We analyzed the genes related to the metabolism of chlorophyll,carotenoids,and flavonoids and the transcription factors(TFs)involved in broccoli yellowing using transcriptome sequencing profiling.Broccoli stored at 10℃showed slight yellowing on postharvest day 5 and serious symptoms on day 12.There were significant changes in chlorophyll fluorescence kinetics,mainly manifesting as a decrease in the Fv/Fm value and an increase in nonphotochemical quenching,during the yellowing process.Transcriptome sequencing profiles from samples of fresh broccoli and broccoli with slight and severe yellowing revealed 6,5,and 4 differentially expressed genes involved in chlorophyll metabolism,carotenoid biosynthesis,and flavonoid biosynthesis,respectively.The transcription factor gene ontology categories showed that the MYB,bHLH,and bZip gene families were involved in chlorophyll metabolism.In addition,the transcription factor families included NACs and ethylene response factors(ERFs)that regulated carotenoid biosynthesis.Reverse transcription polymerase chain reaction further confirmed that bHLH66,PIF4,LOB13,NAC92,and APL were vital transcription factors that potentially regulated the CAO and HYD genes and were involved in chlorophyll metabolism and the carotenoid biosynthetic process.The flavonoid biosynthetic pathway was mainly regulated by MYBs,NACs,WRKYs,MADSs,and bZips.The results of the differentially expressed gene(DEG)and pigment content analyses indicated that the transcriptome data were accurately and positively associated with broccoli yellowing.展开更多
Thermomechanical properties of polyurethanes（PUs） strongly depend on the molecular interactions and microphase structure. In this work, two chain extenders with different ratios, flexile 1,4-butanediol（BDO） and br...Thermomechanical properties of polyurethanes（PUs） strongly depend on the molecular interactions and microphase structure. In this work, two chain extenders with different ratios, flexile 1,4-butanediol（BDO） and branched trimethylolpropane mono allyl ether（TMPAE）, are used to tune the molecular interactions and microphase structures of a series of biodegradable thermoplastic polyurethanes（TPUs）. In TPUs, the biodegradable polycaprolactone（PCL, M_n of 2000） is used as soft segment while 1,6-diisocyanatohexane（HDI） and chain extenders are used as hard segment. Fourier transform infrared spectroscopy（FTIR）, proton nuclear magnetic resonance spectroscppy（~1 H-NMR）, gel permeation chromatography（GPC）, differential scanning calorimetry（DSC）, dynamic mechanical analysis（DMA） and mechanical tests were performed to characterize the bulk structure and properties of TPUs. Compared with BDO, the steric bulk of TMPAE is larger. The increment of TMPAE can help to increase the hydrogen bond content, microphase separation, and the elastic modulus ratio（R）, which would strongly affect the thermomechanical property of the TPUs. The results of this work verify the importance of the structure of chain extender on the properties of TPUs. It provides valuable information for further understanding the structure-property relationships of these polyurethanes.展开更多
IrOx-based catalysts are considered the most promising candidates for oxygen evolution reaction(OER)due to their high efficiency.However,improving their intrinsic catalytic activity is essential for practical applicat...IrOx-based catalysts are considered the most promising candidates for oxygen evolution reaction(OER)due to their high efficiency.However,improving their intrinsic catalytic activity is essential for practical application.In this work,CeO_(2)with three different morphologies(rod,cube,octahedron)and supported IrOx nanoparticles were fabricated,and they display morphology-dependent OER activity.The IrOx/CeO_(2)-rod shows the highest activity;the catalysts have a catalytic activity sequence of rod>cube>octahedron.A plausible mechanism was proposed:the CeO_(2)support with different morphologies modulates the electronic structure of IrOx by the synergistic interaction promoted by oxygen vacancies between the active component and the support,thereby altering the catalytic activity of the IrOx/CeO_(2)catalyst.展开更多
Ecological restoration is one of the hot technologies for the reconstruction of eutrophic lake ecosystems in which the restoration and propagation of submerged plants is the key and difficult step. In this paper, the ...Ecological restoration is one of the hot technologies for the reconstruction of eutrophic lake ecosystems in which the restoration and propagation of submerged plants is the key and difficult step. In this paper, the effect of vermiculite on the growth process of Vallisneria spiralis and sediment microenvironment were investigated, aiming to provide a theoretical basis for the application of vermiculite in aquatic ecological restoration. Results of growth indexes demonstrated that 5% and 10% vermiculite treatment groups statistically promote the growth of Vallisneria spiralis compared to the control. Meanwhile, the results of ecophysiological indexes showed that photosynthetic pigment, soluble sugar content, superoxide dismutase(SOD), and catalase(CAT) activity of 5% and 10% group were increased compared with the control while the malondialdehyde(MDA) content exhibited the opposite result(p < 0.05), which illustrated that vermiculite can improve the resistance of plants and delay the aging process of Vallisneria spiralis. In addition, result of PCA(Principal Component Analysis) demonstrated 5% and 10% group has improved the sediment physical conditions and create more ecological niche for microorganisms directly, and then promoted the growth of plants. The dissolution results showed that vermiculite can dissolve the constant and trace elements needed for plant growth. Furthermore, the addition of vermiculite increased the diversity of microorganisms in the sediments, and promoted the increase of plant growth-promoting bacteria and phosphorus-degrading bacteria. This study could provide a technique reference for the further application of vermiculite in the field of ecological restoration.展开更多
With the widespread adoption of ultrasound guidance,Seldinger puncture techniques,and intracardiac electrical positioning technology for the placement of peripherally inserted central catheters in recent years,an incr...With the widespread adoption of ultrasound guidance,Seldinger puncture techniques,and intracardiac electrical positioning technology for the placement of peripherally inserted central catheters in recent years,an increasing number of medical staff and patients now accept peripheral placement of totally implantable venous access devices(TIVADs)in the upper arm.This approach has the advantage of completely avoiding the risks of hemothorax,pneumothorax,and neck and chest scarring.Medical specialties presently engaged in this study in China include internal medicine,surgery,anesthesiology,and interventional departments.However,command over implantation techniques,treatment of complications,and proper use and maintenance of TIVAD remain uneven among different medical units.Moreover,currently,there are no established quality control standards for implantation techniques or specifications for handling complications.Thus,this expert consensus is proposed to improve the success rate of TIVAD implantation via the upper-arm approach,reduce complication rates,and ensure patient safety.This consensus elaborates on the technical indications and contraindications,procedures and technical points,treatment of complications,and the use and maintenance of upper-arm TIVAD,thus providing a practical reference for medical staff.展开更多
文摘BACKGROUND Sleeve fracture of the patella is an unusual fracture,almost unique to children.The majority of sleeve fractures involve the inferior patellar pole.However,sleeve fractures of the superior pole of the patella are extremely rare in adults.CASE SUMMARY An 18-year-old male patient fell while running in the morning.The patient had tenderness to palpation at the superior pole of the patella,with a palpable gap over the upper part of the patella in both knees.We applied two 4.5-mm suture anchors with the Krackow stitch to repair the sleeve fracture,augmented by autogenous gracilis through performing the figure-of-eight technique.The patient regained approximately the full range of motion of the knee joint without any quadriceps weakness and a normal gait 6 weeks after surgery.CONCLUSION Sleeve fractures of the superior pole of the patella are extremely rare in adults,especially bilateral sleeve fractures.Suture anchors,augmented by autogenous gracilis,provided secure fixation and achieved excellent results in this rare injury.
基金financially supported by the National Natural Science Foundation of China(Nos.52373296 and 52173287)。
文摘Enhancing the lubricating properties and antibacterial adhesion resistance of implantable medical materials is critical to prevent soft tissue injury during implantation and the formation of bacterial biofilms.Prior studies may have exhibited limitations in the preparation methodologies and long-term stability of coatings for implantable medical materials.In this study,we developed a multilayered hybrid hydrogel coating method based on the rate difference of polymerization initiation on the material surface.The acquired coating with persistent lubrication capability retained its functionality after 2×10^(4) cycles of friction and 21 days of PBS immersion.A quaternary ammonium salt coating with antibacterial properties was introduced to further functionalize the coating.Animal experiments demonstrated that this coating exhibited remarkable effects on delaying encrustation and bacterial colonization.These studies indicate that this simple method of introducing lubricating and antibacterial coatings on catheters is likely to enhance the biocompatibility of medical devices and has broad application prospects in this field of medical devices.
基金supported by the Sci-Tech Project Foundation of Guangdong Province(No.2009B080800023)
文摘Objective： To explore the effects and mechanism of glycogen synthase kinase 3β （GSK-313） inhibitor （2＇Z,3＇E）-6-bromo-indirubin-3＇-oxime （BIO） on drug resistance in colon cancer cells. Methods： The colon cancer SW480 and SW620 cells were treated with BIO, 5-fluorouracil （5-FU） and BIO/5-FU, separately. Cell cycle distribution, apoptosis level and efflux ability of rhodamine 123 （Rh123） were detected by flow cytometry. The protein expressions of P-glycoprotein （P-gp）, multidrug resistance protein 2 （MRP2）, thymidylate synthase （TS）, β-catenin, E2F-1 and βcl-2 were detected by Western blot. β-catenin and P-gp were stained with double immunofluorescence and observed under a confocal microscope. Results： BIO up-regulated β-catenin, P-gp, MRP2 and TS, enhanced the efflux ability of Rh123, decreased Bcl-2 protein and gave the opposite effect to E2F-1 protein in SW480 and SW620 ceils. Furthermore, BIO significantly inhibited cell apoptosis, increased S and G2/M phase cells, and reduced the cell apoptosis induced by 5-FU in SW480 cells, whereas the effects were slight or not obvious in SW620 cells. Conclusion： GSK-3β was involved in drug resistance regulation, and activation of β-catenin and inhibition of E2F-1 may be the most responsible for the enhancement of 5-FU chemotherapy resistance induced by GSK-β inhibitor β10 in colon cancer.
基金financially supported by the National Natural Science Foundation of China(Nos.51973134 and 51703139)the State Key Research Development Program of China(No.2020YFC1107000)+2 种基金Fund of Cooperation of Sichuan UniversityDazhou(No.2020CDDZ-14)Chengdu Scientific Program R&D Key Project(No.2019-YF05-00156-SN)the project of the Science and Technology Department in Sichuan Province(No.2020YJ0230)。
文摘Fluorinated diols(FDO)with dangling chain were introduced into polyether urethanes(PEUs)as chain extender in order to achieve internal plasticization.Based on temperature dependent Fourier transform infrared(FTIR)spectroscopy results,the fluorinated polyether urethanes(FPEUs)exhibited weaker hydrogen bonding between C=O and N―H groups with addition of FDO.The crystallinity of hard domain was destroyed determined by thermal analysis.Combined with the results of dynamic mechanical analysis(DMA)and dissipative particle dynamics(DPD)simulation,it was proved that more loosely aggregated hard domains with weakened interaction separated from soft segment were formed.Rheological analysis indicated that FDO reduced the complex viscosity and viscous flow activation energy which endowed the polymer with more flexibility.Despite the loss of hydrogen bonds,there was no significant decline in mechanical property resulting from the decrease of hard segment dissolved in soft segment phase.The presence of fluorinated carbon side chain could function as internal plasticizer and contribute to the processability of polyurethane.
基金supported by the National Natural Science Foundation of China(Grant Nos.92365203,52072168,51861145201,52302180,and 12204232)the National Key Research and Development Program of China(Grant No.2021YFA1202901)+1 种基金the Science and Technology Development Project of Henan Province(Grant No.242102230140)the China Postdoctoral Science Foundation(Grant No.2024M750775)。
文摘Manipulating magnetic domain structure plays a key role in advanced spintronics devices.Theoretical rationale is that the labyrinthine domain structure,normally appearing in ferromagnetic thin films with strong magnetic anisotropy,shows a great potential to increase data storage density for designing magnetic nonvolatile memory and logic devices.However,an electrical control of labyrinthine domain structure remains elusive.Here,we demonstrate the gate-driven evolution of labyrinthine domain structures in an itinerant ferromagnet Cr_(7)Te_(8).By combining electric transport measurements and micromagnetic finite difference simulations,we find that the hysteresis loop of anomalous Hall effect in Cr_(7)Te_(8)samples shows distinct features corresponding to the generation of labyrinthine domain structures.The labyrinthine domain structures are found to be electrically tunable via Li-electrolyte gating,and such gate-driven evolution in Cr_(7)Te_(8)originates from the reduction of the magnetic anisotropic energy with gating,revealed by our micromagnetic simulations.Our results on the gate control of anomalous Hall effect in an itinerant magnetic material provide an opportunity to understand the formation and evolution of labyrinthine domain structures,paving a new route towards electric-field driven spintronics.
文摘With the increasing incidence of breast cancer globally,there is an urgent demand for enhanced diagnostic and therapeutic strategies.This comprehensive review explores advancements in fluorescent probe technology for breast cancer imaging and early diagnosis.Fluorescent probes offer unparalleled sensitivity and specificity in detecting cancer biomarkers,enabling early diagnosis and delineation of tumor margins during surgery.We categorize these probes into molecular,nano,ratiometric,the second near-infrared window(NIR-II),and multifunctional varieties,each with unique capabilities for targeting specific cancer markers.This review highlights recent innovations in probe design,emphasizing their applications in pathogenesis studies,drug development,precision medicine,and fluorescence-guided surgery.Despite their promising clinical potential,challenges such as safety,biomarker targeting accuracy,system compatibility,and clinical translation persist.Addressing these hurdles is crucial for the integration of fluorescent probes into standard cancer care,aiming to improve treatment outcomes and patient quality of life.
文摘Hepatocellular carcinoma （HCC） is one of the most frequent malignant tumors and is the second most common cause of cancer death in China. Therefore, it is very important to detect this disease and the recurrence at its earlier period. Serum tumor markers, as the effective method for detecting hepatocellular carcinoma for a long time, could be divided into 4 categories： oncofetal antigens and glycoprotein antigens; enzymes and isoenzymes; genes; and cytokines. Serum alpha fetoprotein （AFP） is the most widely used tumor marker in detecting patients with hepatocellular carcinoma, and has been proven to have capability of prefiguring the prognosis. However, it has been indicated that AFP-L3 and DCP excel AFP in differentiating hepatocellular carcinoma from nonmalignant hepatopathy and detecting small hepatocellular carcinoma. Some tumor markers, such as human cervical cancer oncogene and human telomerase reverse transcriptase mRNA, have also been indicated to have higher accuracies than AFP. Furthermore, some other tumor markers, such as glypican-3, gamma-glutamyl transferase Ⅱ, alpha-Ifucosidase, transforming growth factor-beta1, tumorspecific growth factor, have been indicated to be available supplementaries to AFP in the detection. AFP mRNA has been shown to correlate with the metastasis and recurrence of HCC, and it may be the most useful marker to prefigure the prognosis. Some other markers, such as gamma-glutamyl transferase mRNA, vascular endothelial growth factor, and interleukin-8, could also be used as available prognostic indicators, and the simultaneous determination of AFP and these markers may detect the recurrence of HCC at its earlier period.
基金supported by the National Important Special Project of Science and Technology of China(No.GZH200600301)
文摘Differences between the Cenozoic and pre-Cenozoic strata and structures in the middlesouth South Yellow Sea are analyzed using high-resolution airborne gravity data combined with data from offshore wells, seismic exploration and the regional geological background, using forward and inverse methods. We discuss why hydrocarbon exploration has so far failed in the South Yellow Sea, and put forward alternative future exploration plans. The results show that there are thick Cenozoic strata over Jurassic-Cretaceous continental strata in the southern basin of the South Yellow Sea, contrasted with thick Mid-Paleozoic marine strata preserved in the middle uplift area. In the mid-southern South Yellow Sea, airborne Bouguer gravity anomalies are fragmentized with scattered local anomalies. Many tensile normal faults and minor fault blocks occur in the Cenozoic strata. In contrast, reverse faults and nappe structures are found in pre-Cenozoic strata. The essential reasons for the lack of a breakthrough in hydrocarbon exploration are the complexity of geological structures, hydrocarbon accumulation environments, seismic-geologic conditions, and the difficulty of interpreting and understanding these features, rather than an imbalance of exploration framework. Hydrocarbon exploration should be targeted at Mid-Cenozoic continental strata, especially of Paleogene age, in the middle to southern parts of the South Yellow Sea. Special attention should be paid to the favorable structural belts SYI1, SYI2, SYI3 and SYI4 in the southern basin of the South Yellow Sea, where detailed seismic exploration or drilling should be carried out. Attention should also be given to Mid-Paleozoic marine strata in the middle uplift area of the South Yellow Sea. Key strategies that could lead to a hydrocarbon exploration breakthrough are improving exploration resolution, conducting detailed studies of fine structures, and accurately locating minor structures.
文摘The underwater topography in the offshore sea area of north Jiangsu is complicated, including the middle radial sand ridges and northern Haizhou bay underwater shoal. Therefore, it forms special marine dynamic conditions and typical shoal wetland ecosystem. Previous researches of these sea areas were mainly focused on the forms of morphogenesis and the characteristic of conformation of sand ridges. Few studies have done on three dimensional hydrodynamic and water quality simulation. This paper introduced EFDC (Environ-mental Fluid Dynamics Code) to study the tidal current fields, dilution and diffusion of the sewage outlet near Yangkou Port. Comparison between computation results and the observed data indicates that this model could reasonably simulate hydrodynamic fields. Based on the computed tidal current field, the distributions of COD concentration were simulated. The range of contamination diffusion derived from sewage outlet was very limited, and the influence range of sewage came to the maximum when ebb slacks in neap tide period.
基金supported by the National Key R&D Program of China(no.2016YFD0400103).
文摘Postharvest broccoli is prone to yellowing during storage,which is the key factor leading to a reduction in value.To explore appropriate control methods,it is important to understand the mechanisms of yellowing.We analyzed the genes related to the metabolism of chlorophyll,carotenoids,and flavonoids and the transcription factors(TFs)involved in broccoli yellowing using transcriptome sequencing profiling.Broccoli stored at 10℃showed slight yellowing on postharvest day 5 and serious symptoms on day 12.There were significant changes in chlorophyll fluorescence kinetics,mainly manifesting as a decrease in the Fv/Fm value and an increase in nonphotochemical quenching,during the yellowing process.Transcriptome sequencing profiles from samples of fresh broccoli and broccoli with slight and severe yellowing revealed 6,5,and 4 differentially expressed genes involved in chlorophyll metabolism,carotenoid biosynthesis,and flavonoid biosynthesis,respectively.The transcription factor gene ontology categories showed that the MYB,bHLH,and bZip gene families were involved in chlorophyll metabolism.In addition,the transcription factor families included NACs and ethylene response factors(ERFs)that regulated carotenoid biosynthesis.Reverse transcription polymerase chain reaction further confirmed that bHLH66,PIF4,LOB13,NAC92,and APL were vital transcription factors that potentially regulated the CAO and HYD genes and were involved in chlorophyll metabolism and the carotenoid biosynthetic process.The flavonoid biosynthetic pathway was mainly regulated by MYBs,NACs,WRKYs,MADSs,and bZips.The results of the differentially expressed gene(DEG)and pigment content analyses indicated that the transcriptome data were accurately and positively associated with broccoli yellowing.
基金financially supported by the National Natural Science Foundation of China (No. 51573114)the National Science Fund for Distinguished Young Scholars of China (No. 51425305)the Project of State Key Laboratory of Polymer Materials Engineering (Sichuan University) (No. SKLPME 2016-2-04)
文摘Thermomechanical properties of polyurethanes（PUs） strongly depend on the molecular interactions and microphase structure. In this work, two chain extenders with different ratios, flexile 1,4-butanediol（BDO） and branched trimethylolpropane mono allyl ether（TMPAE）, are used to tune the molecular interactions and microphase structures of a series of biodegradable thermoplastic polyurethanes（TPUs）. In TPUs, the biodegradable polycaprolactone（PCL, M_n of 2000） is used as soft segment while 1,6-diisocyanatohexane（HDI） and chain extenders are used as hard segment. Fourier transform infrared spectroscopy（FTIR）, proton nuclear magnetic resonance spectroscppy（~1 H-NMR）, gel permeation chromatography（GPC）, differential scanning calorimetry（DSC）, dynamic mechanical analysis（DMA） and mechanical tests were performed to characterize the bulk structure and properties of TPUs. Compared with BDO, the steric bulk of TMPAE is larger. The increment of TMPAE can help to increase the hydrogen bond content, microphase separation, and the elastic modulus ratio（R）, which would strongly affect the thermomechanical property of the TPUs. The results of this work verify the importance of the structure of chain extender on the properties of TPUs. It provides valuable information for further understanding the structure-property relationships of these polyurethanes.
基金Project supported by the National Natural Science Foundation of China(21522106.21971117)111 Project(B18030)from China+2 种基金the Open Fund of the State Key Laboratory of Rare Earth Resource Utilization(RERU2019001)the Functional Research Fund for the Central Universities,Nankai University(ZB19500202)Beijing-Tianjin-Hebei Collaborative Innovation Project(63201058).
文摘IrOx-based catalysts are considered the most promising candidates for oxygen evolution reaction(OER)due to their high efficiency.However,improving their intrinsic catalytic activity is essential for practical application.In this work,CeO_(2)with three different morphologies(rod,cube,octahedron)and supported IrOx nanoparticles were fabricated,and they display morphology-dependent OER activity.The IrOx/CeO_(2)-rod shows the highest activity;the catalysts have a catalytic activity sequence of rod>cube>octahedron.A plausible mechanism was proposed:the CeO_(2)support with different morphologies modulates the electronic structure of IrOx by the synergistic interaction promoted by oxygen vacancies between the active component and the support,thereby altering the catalytic activity of the IrOx/CeO_(2)catalyst.
基金financially supported by the National Natural Science Foundation of China (Nos. 5170925431830013)+1 种基金the Youth Innovation Promotion Association, Chinese Academy of Sciences (No. 2020335)the Natural Science Foundation of Hubei Province (No. 2020CFB555)。
文摘Ecological restoration is one of the hot technologies for the reconstruction of eutrophic lake ecosystems in which the restoration and propagation of submerged plants is the key and difficult step. In this paper, the effect of vermiculite on the growth process of Vallisneria spiralis and sediment microenvironment were investigated, aiming to provide a theoretical basis for the application of vermiculite in aquatic ecological restoration. Results of growth indexes demonstrated that 5% and 10% vermiculite treatment groups statistically promote the growth of Vallisneria spiralis compared to the control. Meanwhile, the results of ecophysiological indexes showed that photosynthetic pigment, soluble sugar content, superoxide dismutase(SOD), and catalase(CAT) activity of 5% and 10% group were increased compared with the control while the malondialdehyde(MDA) content exhibited the opposite result(p < 0.05), which illustrated that vermiculite can improve the resistance of plants and delay the aging process of Vallisneria spiralis. In addition, result of PCA(Principal Component Analysis) demonstrated 5% and 10% group has improved the sediment physical conditions and create more ecological niche for microorganisms directly, and then promoted the growth of plants. The dissolution results showed that vermiculite can dissolve the constant and trace elements needed for plant growth. Furthermore, the addition of vermiculite increased the diversity of microorganisms in the sediments, and promoted the increase of plant growth-promoting bacteria and phosphorus-degrading bacteria. This study could provide a technique reference for the further application of vermiculite in the field of ecological restoration.
文摘With the widespread adoption of ultrasound guidance,Seldinger puncture techniques,and intracardiac electrical positioning technology for the placement of peripherally inserted central catheters in recent years,an increasing number of medical staff and patients now accept peripheral placement of totally implantable venous access devices(TIVADs)in the upper arm.This approach has the advantage of completely avoiding the risks of hemothorax,pneumothorax,and neck and chest scarring.Medical specialties presently engaged in this study in China include internal medicine,surgery,anesthesiology,and interventional departments.However,command over implantation techniques,treatment of complications,and proper use and maintenance of TIVAD remain uneven among different medical units.Moreover,currently,there are no established quality control standards for implantation techniques or specifications for handling complications.Thus,this expert consensus is proposed to improve the success rate of TIVAD implantation via the upper-arm approach,reduce complication rates,and ensure patient safety.This consensus elaborates on the technical indications and contraindications,procedures and technical points,treatment of complications,and the use and maintenance of upper-arm TIVAD,thus providing a practical reference for medical staff.
