The nonlinear traveling wave vibration of rotating ferromagnetic functionally graded(FG)cylindrical shells under multi-physics fields is investigated.Grounded in the Kirchhoff-Love thin shell theory,the geometric nonl...The nonlinear traveling wave vibration of rotating ferromagnetic functionally graded(FG)cylindrical shells under multi-physics fields is investigated.Grounded in the Kirchhoff-Love thin shell theory,the geometric nonlinearity is incorporated into the model,and the constitutive equations are derived.The physical parameters of functionally graded materials(FGMs),which exhibit continuous variation across the thickness gradient,are of particular interest.The nonlinear magneto-thermoelastic governing equations are derived in accord with Hamilton's principle.The nonlinear partial differential equations are discretized with the Galerkin method,and the analytical expression of traveling wave frequencies is derived with an approximate method.The accuracy of the proposed method is validated through the comparison with the results from the literature and numerical solutions.Finally,the visualization analyses are conducted to examine the effects of key parameters on the traveling wave frequencies.The results show that the factors including the power-law index,temperature,magnetic field intensity,and rotating speed have the coupling effects with respect to the nonlinear vibration behavior.展开更多
Cardiac troponin I(cTnI),a widely used biomarker for assessing cardiovascular risk,can provide a window for the evaluation of drug-induced myocardial injury.Label-free biosensors are promising candidates for detecting...Cardiac troponin I(cTnI),a widely used biomarker for assessing cardiovascular risk,can provide a window for the evaluation of drug-induced myocardial injury.Label-free biosensors are promising candidates for detecting cell secretomes,since they do not require labor-intensive processes.In this work,a label-free electrochemical aptasensor is developed for in situ monitoring of cardiac cell secre-tomes in cell culture media based on target-induced strand displacement.The aptasensing system contains an aptamer-functionalized signal nanoprobe facing trimetallic metal-organic framework nanosheets and a gold nanoparticle-based detection working electrode modified with DNA nanotetrahedron-based complementary DNA for indirect target detection.The signal nanoprobes(termed CAHA)consisted of copper-based metal-organic frameworks,AuPt nanoparticles,horseradish peroxidase,and an aptamer.When the aptasensor is exposed to cardiac cell secretomes,cTnI competi-tively binds to the aptamer,resulting in the release of signal nanoprobes from the biorecognition interface and electrochemical signal changes.The aptasensor exhibited rapid response times,a low detection limit of 0.31 pg/mL,and a wide linear range of 0.001-100 ng/mL.We successfully used this aptasensor to measure cTnI concentrations among secreted cardiac markers during antitumor drug treatment.In general,aptasensors can be used to monitor a variety of cardiac biomarkers in the eval-uation of cardiotoxicity.展开更多
As an aging-associated degenerative disease,Alzheimer’s disease is characterized by the deposition of amyloid beta(Aβ),oxidative stress,inflammation,dysfunction and loss of cholinergic neurons.Colla Corii Asini(CCA)...As an aging-associated degenerative disease,Alzheimer’s disease is characterized by the deposition of amyloid beta(Aβ),oxidative stress,inflammation,dysfunction and loss of cholinergic neurons.Colla Corii Asini(CCA)is a traditional Chinese medicine which has been used for feebleness-related diseases and anti-aging.CCA might delay aging-induced degenerative changes in neurons.In the present study,we evaluated antioxidant activity,cytoprotective effects,and Aβremovability of enzyme-digested Colla Corii Asini(CCAD).Oxygen radical absorbance capacity(ORAC)activity assay showed that,as compared to gelatins from the skin of porcine,bovine and cold water fish,CCA exhibited the highest ORAC activity.The ORAC activity of CCA and CCAD was increased gradually by the length of time in storage.Ultrastructure analysis by scanning electron microscopy showed that among CCA manufactured in 2008,2013,2017 and gelatin from cold water fish skin,CCA manufactured in 2008 presented the smoothest surface structure.We further tested the protective effects of CCAD(manufactured in 2008)and enzyme-digested gelatin from cold water fish skin(FGD)on hydrogen peroxide(H2O2)-induced cell death in nerve growth factor-differentiated neuronal-like PC12 cells.Presto blue assay showed that both FGD and CCAD at 0.5 mg/m L increased cell viability in H2O2-treated neuronal-like PC12 cells.The protection of CCAD was significantly superior to that of FGD.Acetylcholinesterase(Ach E)assay showed that both FGD and CCAD inhibited Ach E activity in nerve growth factor-differentiated neuronal-like PC12 cells to 89.1%and 74.5%of that in non-treated cells,respectively.The data suggest that CCAD might be able to increase the neurotransmitter acetylcholine.Although CCAD inhibited Ach E activity in neuronal-like PC12 cells,CCAD prevented H2O2-induced abnormal deterioration of Ach E.ELISA and neprilysin activity assay results indicated that CCAD reduced amyloid beta accumulation and increased neprilysin activity in Aβ1–42-treated neuronal-like PC12 cells,suggesting that CCAD can enhance Aβclearance.Our results suggest that CCA might be useful for preventing and treating Alzheimer’s disease.展开更多
BACKGROUND The hereditary antithrombin(AT)deficiency caused by SERPINC1 gene mutation is an autosomal dominant thrombotic disorder.An increasing number of studies have shown that mutations in the SERPINC1 rs2227589 po...BACKGROUND The hereditary antithrombin(AT)deficiency caused by SERPINC1 gene mutation is an autosomal dominant thrombotic disorder.An increasing number of studies have shown that mutations in the SERPINC1 rs2227589 polymorphic site are correlated with a risk of venous thromboembolism(VTE)at common sites,such as lower extremity deep venous thrombosis and pulmonary thromboembolism.Currently,there are no reports of cerebral venous sinus thrombosis(CVST),a VTE site with a low incidence rate and rs2227589 polymorphism.CASE SUMMARY Here,we report a Chinese CVST case with a mutation of the SERPINC1 rs2227589 polymorphic site,which did not cause significant AT deficiency.In a 50-year-old male patient presenting with multiple cerebral venous sinus thromboses no predisposing factors were detected,although a relative had a history of lower extremity deep venous thrombosis.We performed sequencing of the SERPINC1 gene for the patient and his daughter,which revealed the same heterozygous mutation at the rs2227589 polymorphic site:c.41+141G>A.CONCLUSION The results showed that more studies should be conducted to assess the correlation between rs2227589 polymorphism and CVST.展开更多
In this article, two split-step finite difference methods for Schrodinger-KdV equations are formulated and investigated. The main features of our methods are based on:(i) The applications of split-step technique for S...In this article, two split-step finite difference methods for Schrodinger-KdV equations are formulated and investigated. The main features of our methods are based on:(i) The applications of split-step technique for Schrodingerlike equation in time.(ii) The utilizations of high-order finite difference method for KdV-like equation in spatial discretization.(iii) Our methods are of spectral-like accuracy in space and can be realized by fast Fourier transform efficiently. Numerical experiments are conducted to illustrate the efficiency and accuracy of our numerical methods.展开更多
基金supported by the National Natural Science Foundation of China(No.12172321)。
文摘The nonlinear traveling wave vibration of rotating ferromagnetic functionally graded(FG)cylindrical shells under multi-physics fields is investigated.Grounded in the Kirchhoff-Love thin shell theory,the geometric nonlinearity is incorporated into the model,and the constitutive equations are derived.The physical parameters of functionally graded materials(FGMs),which exhibit continuous variation across the thickness gradient,are of particular interest.The nonlinear magneto-thermoelastic governing equations are derived in accord with Hamilton's principle.The nonlinear partial differential equations are discretized with the Galerkin method,and the analytical expression of traveling wave frequencies is derived with an approximate method.The accuracy of the proposed method is validated through the comparison with the results from the literature and numerical solutions.Finally,the visualization analyses are conducted to examine the effects of key parameters on the traveling wave frequencies.The results show that the factors including the power-law index,temperature,magnetic field intensity,and rotating speed have the coupling effects with respect to the nonlinear vibration behavior.
基金the National Natural Science Foundation of China(GrantNos.:82373841,82003710)Basic and Applied Basic Research Foundationof Guangdong Province(Grant No.:2024A1515011385)+1 种基金Guangzhou Basic and Applied Basic Research Foundation(Grant No.:2025A04J7083)Grant from the GuangdongPharmaceutical University(Grant No.:2024QZ01).
文摘Cardiac troponin I(cTnI),a widely used biomarker for assessing cardiovascular risk,can provide a window for the evaluation of drug-induced myocardial injury.Label-free biosensors are promising candidates for detecting cell secretomes,since they do not require labor-intensive processes.In this work,a label-free electrochemical aptasensor is developed for in situ monitoring of cardiac cell secre-tomes in cell culture media based on target-induced strand displacement.The aptasensing system contains an aptamer-functionalized signal nanoprobe facing trimetallic metal-organic framework nanosheets and a gold nanoparticle-based detection working electrode modified with DNA nanotetrahedron-based complementary DNA for indirect target detection.The signal nanoprobes(termed CAHA)consisted of copper-based metal-organic frameworks,AuPt nanoparticles,horseradish peroxidase,and an aptamer.When the aptasensor is exposed to cardiac cell secretomes,cTnI competi-tively binds to the aptamer,resulting in the release of signal nanoprobes from the biorecognition interface and electrochemical signal changes.The aptasensor exhibited rapid response times,a low detection limit of 0.31 pg/mL,and a wide linear range of 0.001-100 ng/mL.We successfully used this aptasensor to measure cTnI concentrations among secreted cardiac markers during antitumor drug treatment.In general,aptasensors can be used to monitor a variety of cardiac biomarkers in the eval-uation of cardiotoxicity.
基金supported in part by the Japan Society for the Promotion of Science(JSPS)Grant-in-Aid for Scientific Research,No.17K11813(to LX)Tai Shan Industrial Experts Program of China No.TSCY20170233(to FL)。
文摘As an aging-associated degenerative disease,Alzheimer’s disease is characterized by the deposition of amyloid beta(Aβ),oxidative stress,inflammation,dysfunction and loss of cholinergic neurons.Colla Corii Asini(CCA)is a traditional Chinese medicine which has been used for feebleness-related diseases and anti-aging.CCA might delay aging-induced degenerative changes in neurons.In the present study,we evaluated antioxidant activity,cytoprotective effects,and Aβremovability of enzyme-digested Colla Corii Asini(CCAD).Oxygen radical absorbance capacity(ORAC)activity assay showed that,as compared to gelatins from the skin of porcine,bovine and cold water fish,CCA exhibited the highest ORAC activity.The ORAC activity of CCA and CCAD was increased gradually by the length of time in storage.Ultrastructure analysis by scanning electron microscopy showed that among CCA manufactured in 2008,2013,2017 and gelatin from cold water fish skin,CCA manufactured in 2008 presented the smoothest surface structure.We further tested the protective effects of CCAD(manufactured in 2008)and enzyme-digested gelatin from cold water fish skin(FGD)on hydrogen peroxide(H2O2)-induced cell death in nerve growth factor-differentiated neuronal-like PC12 cells.Presto blue assay showed that both FGD and CCAD at 0.5 mg/m L increased cell viability in H2O2-treated neuronal-like PC12 cells.The protection of CCAD was significantly superior to that of FGD.Acetylcholinesterase(Ach E)assay showed that both FGD and CCAD inhibited Ach E activity in nerve growth factor-differentiated neuronal-like PC12 cells to 89.1%and 74.5%of that in non-treated cells,respectively.The data suggest that CCAD might be able to increase the neurotransmitter acetylcholine.Although CCAD inhibited Ach E activity in neuronal-like PC12 cells,CCAD prevented H2O2-induced abnormal deterioration of Ach E.ELISA and neprilysin activity assay results indicated that CCAD reduced amyloid beta accumulation and increased neprilysin activity in Aβ1–42-treated neuronal-like PC12 cells,suggesting that CCAD can enhance Aβclearance.Our results suggest that CCA might be useful for preventing and treating Alzheimer’s disease.
基金Supported by the Affiliated Hospital of Guangdong Medical University,No.LCYJ2018C009.
文摘BACKGROUND The hereditary antithrombin(AT)deficiency caused by SERPINC1 gene mutation is an autosomal dominant thrombotic disorder.An increasing number of studies have shown that mutations in the SERPINC1 rs2227589 polymorphic site are correlated with a risk of venous thromboembolism(VTE)at common sites,such as lower extremity deep venous thrombosis and pulmonary thromboembolism.Currently,there are no reports of cerebral venous sinus thrombosis(CVST),a VTE site with a low incidence rate and rs2227589 polymorphism.CASE SUMMARY Here,we report a Chinese CVST case with a mutation of the SERPINC1 rs2227589 polymorphic site,which did not cause significant AT deficiency.In a 50-year-old male patient presenting with multiple cerebral venous sinus thromboses no predisposing factors were detected,although a relative had a history of lower extremity deep venous thrombosis.We performed sequencing of the SERPINC1 gene for the patient and his daughter,which revealed the same heterozygous mutation at the rs2227589 polymorphic site:c.41+141G>A.CONCLUSION The results showed that more studies should be conducted to assess the correlation between rs2227589 polymorphism and CVST.
基金Supported by the National Natural Science Foundation of China under Grant No.11571181
文摘In this article, two split-step finite difference methods for Schrodinger-KdV equations are formulated and investigated. The main features of our methods are based on:(i) The applications of split-step technique for Schrodingerlike equation in time.(ii) The utilizations of high-order finite difference method for KdV-like equation in spatial discretization.(iii) Our methods are of spectral-like accuracy in space and can be realized by fast Fourier transform efficiently. Numerical experiments are conducted to illustrate the efficiency and accuracy of our numerical methods.
