Gastric Carcinoma(GC)is a highly fatal malignant tumor with a poor prognosis.Its elevated mortality rates are primarily due to its proclivity for late-stage metastasis.Exploring the metabolic interactions between tumo...Gastric Carcinoma(GC)is a highly fatal malignant tumor with a poor prognosis.Its elevated mortality rates are primarily due to its proclivity for late-stage metastasis.Exploring the metabolic interactions between tumor microenvironment and the systemic bloodstream could help to clearly understand the mechanisms and identify precise biomarkers of tumor growth,proliferation,and metastasis.In this study,an integrative approach that combines plasma metabolomics with mass spectrometry imaging of tumor tissue was developed to investigate the global metabolic landscape of GC tumorigenesis and metastasis.The results showed that the oxidized glutathione to glutathione ratio(GSSH/GSH)became increased in non-distal metastatic GC(M0),which means an accumulation of oxidative stress in tumor tissues.Furthermore,it was found that the peroxidation of polyunsaturated fatty acids,such as 9,10-EpOMe,9-HOTrE,etc.,were accelerated in both plasma and tumor tissues of distal metastatic GC(M1).These changes were further confirmed the potential effect of CYP2E1 and GGT1 in metastatic potential of GC by mass spectrometry imaging(MSI)and immunohistochemistry(IHC).Collectively,our findings reveal the integrated multidimensional metabolomics approach is a clinical useful method to unravel the bloodtumor metabolic crosstalk,illuminate reprogrammed metabolic networks,and provide reliable circulating biomarkers.展开更多
The global rise in animal protein consumption has significantly amplified the demand for fodder.A comprehensive understanding of the diversity and characteristics of existing fodder resources is essential for balanced...The global rise in animal protein consumption has significantly amplified the demand for fodder.A comprehensive understanding of the diversity and characteristics of existing fodder resources is essential for balanced nutritional fodder production.This study investigates the diversity and composition of fodder plants and identifies key species for cattle in Zhaotong City,Yunnan,China,while documenting indigenous knowledge on their usage and selection criteria.Ethnobotanical surveys were conducted in 19 villages across seven townships with 140 informants.Data were collected through semi-structured interviews,free listing,and participatory observation,and analyzed using Relative Frequency Citation.A total of 125 taxa(including 106 wild and 19 cultivated)were reported.The most cited family is Poaceae(27 taxa,21.43%),followed by Asteraceae(17 taxa,13.49%),Fabaceae(14 taxa,11.11%),Polygonaceae(9 taxa,7.14%)and Lamiaceae(4 taxa,3.17%).The whole plant(66.04%)and herbaceous plants(84.80%)were the most used parts and life forms.The most cited species were Zea mays,Brassica rapa,Solanum tuberosum,Eragrostis nigra,and Artemisia dubia.Usage of diverse fodder resources reflects local wisdom in managing resource availability and achieving balanced nutrition while coping with environmental and climatic risks.Preferences for certain taxonomic groups are due to their quality as premier fodder resources.To promote integrated crop-livestock farming,we suggest further research into highly preferred fodder species,focusing on nutritional assessment,digestibility,meat quality impacts,and potential as antibiotic alternatives.Establishing germplasm and gene banks for fodder resources is also recommended.展开更多
Background and Objective Inflammation plays a pivotal role in the progression of coronary artery disease(CAD).High-sensitivity C-reactive protein(hsCRP)serves as a well-established biomarker for assessing cardiovascul...Background and Objective Inflammation plays a pivotal role in the progression of coronary artery disease(CAD).High-sensitivity C-reactive protein(hsCRP)serves as a well-established biomarker for assessing cardiovascular inflammation risk.However,the specific intestinal microbiota alteration contributing to increased inflammation remains unclear.Therefore,the present study investigated the correlation between the intestinal microbiota and inflammation in patients with unstable angina(UA).Methods A cohort of 92 patients with UA was recruited for this study.The plasma hsCRP level was measured via a CardioPhase hsCRP assay,fecal samples were collected after admission,and 16S rRNA sequencing was conducted to identify the fecal microbial profile.The participants were classified into two groups according to the median hsCRP level(1.11 mg/L).The composition of the fecal microbiota was compared between patients with hsCRP≥1.11 mg/L and those with hsCRP<1.11 mg/L.Additionally,the correlations between the fecal microbiota and clinical characteristics were analyzed.Results A notable reduction in the relative abundance of Akkermansia was observed in patients with hsCRP≥1.11 mg/L,whereas the diversity of the fecal microbiota was not significantly different between patients with hsCRP≥1.11 mg/L and those with hsCRP<1.11 mg/L.Furthermore,the abundance of Akkermansia was negatively correlated with hsCRP levels.Conclusion This study suggested a significant association between decreased levels of Akkermansia and inflammatory risk in patients with UA.These findings underscore the potential role of the intestinal microbiota in contributing to inflammation in UA patients.Further work is needed on the mechanism by which the microbiota contributes to inflammatory risk.展开更多
The present study was conducted to examine the trophic transfer of potentially toxic elements(PTEs)in a closed arsenic mine.Eight PTEs in a soil-plant-leaf litter-earthworm-top predators(free-range local chicken and w...The present study was conducted to examine the trophic transfer of potentially toxic elements(PTEs)in a closed arsenic mine.Eight PTEs in a soil-plant-leaf litter-earthworm-top predators(free-range local chicken and wild passerine bird)system were analyzed for nitrogen and carbon stable isotopes,PTE concentrations,bioaccumulation factors(BAFs),and transfer factors(TFs).The PTE concentrations in soils from mining areas were generally higher than a adjacent controlled area,with As and Cd in soils showing the prominent compared to other six PTEs,as seen for the indices of geo-accumulation index(I_(geo)),pollution index(PI)and potential ecological risk index(RI).The relatively high BAF and TF values suggested a distinct biotransfer of PTEs along the soil-plant-leaf litter-earthworm system.BAFs were mostly<1 except in earthworms,indicating that earthworms had a strong capacity to take up these metals.The TFs varied both among PTEs and organism’s species,e.g.,the transfer capacities of As in Pteris vittata and Pteris cretica,Cd in Miscanthus sinensis,and Pb,Cr and Mn in moss were the highest.For local free-range chicken and wild passerine bird,the concentrations of PTEs were higher in gastric contents and feather than in internal tissue(stomach,liver and heart),with lower contents in muscle and egg.Bioaccumulation of PTEs generally decreased from decomposer earthworms,to primary producer plants,to top predator,indicating a potential bio-dilution tendency in higher trophic levels in the terrestrial food chain.展开更多
<strong>Objective:</strong> To assess and compare the clinical application value of nursing risk management in the diagnosis and treatment of severe peptic ulcer bleeding. <strong>Methods:</strong...<strong>Objective:</strong> To assess and compare the clinical application value of nursing risk management in the diagnosis and treatment of severe peptic ulcer bleeding. <strong>Methods:</strong> A total of 70 patients with severe peptic ulcer bleeding were selected as the research objects, randomly divided into observation group and control group, 35 cases for each. The nursing risk management and conventional nursing management were implemented for the two groups, respectively. The success rate of hemostasis, average length of stay, cure rate, complication rate, dyspnea rate, nursing dispute rate, SAS score, and SDS score were compared between the two groups. Patients’ satisfaction with nursing quality was also compared. <strong>Results:</strong> After the implementation of two different nursing management methods, all the above-mentioned metrics were better in the observation group than in the control group, and the differences were statistically significant (P < 0.01 or P < 0.05). <strong>Conclusion:</strong> The application of nursing risk management in the nursing intervention process of patients with severe peptic ulcer bleeding can effectively reduce the probability of nursing risk events, increase the cure rate, shorten the patient’s hospital stay, but also improve the patients’ psychological state and increase the patient’s satisfaction with nursing quality, which is worth clinical promotion.展开更多
Objective: To explore the application effect of oral glucose combined with short-term intravenous nutrition in preventing postoperative hypoglycemia after painless endoscopic gastric polyp resection, and to provide gu...Objective: To explore the application effect of oral glucose combined with short-term intravenous nutrition in preventing postoperative hypoglycemia after painless endoscopic gastric polyp resection, and to provide guidance for better management of patients undergoing such procedures. Methods: A total of 886 patients who underwent painless endoscopic gastric polyp resection in the Department of Gastroenterology, the First Affiliated Hospital of Yangtze University, from January 2020 to December 2021, were selected as the study subjects. According to the random number table method, they were divided into an experimental group and a control group, with 443 cases in each group. Patients in the control group were subjected to routine fasting and water restriction for 8 hours before surgery, and routine fasting for 24 hours after surgery. Short-term intravenous nutrition support was provided through fluid supplementation, and finger blood glucose levels were monitored at 1 hour, 4 hours, and 8 hours after completion of intravenous infusion, or when symptoms such as palpitations and cold sweats occurred. The experimental group received oral administration of 5% glucose solution (500 ml) 2 hours before surgery based on the procedures of the control group. The incidence of preoperative discomfort (hunger, thirst, and fatigue), residual gastric fluid volume, and postoperative hypoglycemia were compared between the two groups. Results: The incidence of postoperative blood glucose Conclusion: Based on the study population, oral glucose combined with short-term intravenous nutrition can effectively prevent the incidence of hypoglycemia in patients undergoing painless endoscopic gastric polyp resection. However, due to the limitations of a single-center study and a small number of cases, its feasibility needs to be further validated with more data from multi-center and large-sample cases.展开更多
Background/Aim: Conventional treatment for hypertrophic anal papillae (HAP) has effectiveness and safety concerns. This study aimed to investigate the feasibility, safety, and efficacy of transparent cap-assisted endo...Background/Aim: Conventional treatment for hypertrophic anal papillae (HAP) has effectiveness and safety concerns. This study aimed to investigate the feasibility, safety, and efficacy of transparent cap-assisted endoscopic hot snare resection for the treatment of HAP. Methods: Endoscopic and clinical data of patients treated with transparent cap-assisted endoscopic hot snare resection for HAP at the Department of Gastroenterology, First Affiliated Hospital of Yangtze University from June 2019 to June 2021, were collected and retrospectively analyzed. Results: A total of 56 patients with HAP were treated with transparent cap-assisted endoscopic hot snare resection, including 53 patients (94.6%) with single hypertrophic anal papillae and 3 patients (5.4%) with multiple HAP;51 patients (83.6%) with basal diameter <5 mm and 10 patients (16.4%) with basal diameter ≥5 mm. The procedures that were performed together with transparent cap-assisted endoscopic hot snare resection for HAP treatment included total colon examination in 56 cases (100%), endoscopic polypectomy in 20 cases (35.7%), endoscopic sclerotherapy for hemorrhoids in 29 cases (51.8%), and endoscopic rubber band ligation for hemorrhoids in 11 cases (19.6%). No patient experienced bleeding during or after HAP with transparent cap-assisted endoscopic hot snare resection. Pain level was evaluated by the visual analog score (VAS) method. 52 patients (92.9%) did not have pain and 4 patients (7.1%) had mild pain 3 days after surgery. At a postoperative follow-up of 2 to 18 months, patient satisfaction with transparent cap-assisted endoscopic hot snare resection for HAP treatment was 100% (56/56). Conclusion: Transparent cap-assisted endoscopic hot snare resection is safe and effective for treating HAP.展开更多
BACKGROUND： It has been previously demonstrated that the neural cell microenvironment has the ability to induce differentiation of bone marrow mesenchymal stem cells （BMSCs） into the neural cells. OBJECTIVE： To es...BACKGROUND： It has been previously demonstrated that the neural cell microenvironment has the ability to induce differentiation of bone marrow mesenchymal stem cells （BMSCs） into the neural cells. OBJECTIVE： To establish a co-culture system of human BMSCs and neural cells, and to observe effects of this co-culture system on differentiation of human BMSCs into neural cells. DESIGN, TIME AND SETTING： A comparative observation experiment, performed at the Center Labora-tory of the Affiliated Hospital of Medical College Qingdao University from October 2006 to December 2007. MATERIALS： Neural cells were obtained from human fetal brain tissue. BMSCs were harvested from fe-male patients that underwent autonomous stem cell transplantation. METHODS： BMSCs in the co-culture group consisted of BMSCs and third passage neural cells. BMSCs in the control group were solely cultured in vitro. MAIN OUTCOME MEASURES： Morphological changes of BMSCs were observed, and expression of the neuronal specific marker, neuron-specific enolase （NSE）, was analyzed by immunofluorescence staining after 4-5-day co-culture. RESULTS： The number of neural cells in the co-culture group increased and the cells spread on the culture bottle surface. Radial dendrite formed and connected with each other. NSE-immunoreactive cells were also detected. The positive ratio of NSE-positive cells reached （32.7±11.5）%, with morphological characteristics similar to neuronal cells. Human BMSCs did not express NSE in the control group. CONCLUSION： The microenvironment provided by neurons induced differentiation of BMSCs into neu-ronal-like cells.展开更多
Since changes in mechanical properties of biological tissues are often closely related to pathology,the viscoelastic properties are important physical parameters for medical diagnosis.A photoacoustic(PA)phase-resolved...Since changes in mechanical properties of biological tissues are often closely related to pathology,the viscoelastic properties are important physical parameters for medical diagnosis.A photoacoustic(PA)phase-resolved method for noninvasively characterizing the biological tissue viscoelasticity has been proposed by Gao et al.[G.Gao,S.Yang,D.Xing,\Viscoelasticity imaging of biological tissues with phase-resolved photoacoustic measurement,"Opt.Lett.36,3341–3343(2011)].The mathematical relationship between the PA phase delay and the viscosity–elasticity ratio has been theoretically deduced.Moreover,systems of PA viscoelasticity(PAVE)imaging including PAVE microscopy and PAVE endoscopy were developed,and high-PA-phase contrast images re°ecting the tissue viscoelasticity information have been successfully achieved.The PAVE method has been developed in tumor detection,atherosclerosis characterization and related vascular endoscopy.We reviewed the development of the PAVE technique and its applications in biomedical¯elds.It is believed that PAVE imaging is of great potential in both biomedical applications and clinical studies.展开更多
Smoking is a well-known risk factor of reproductive health. However, the effect of paternal smoking on fertility has been less extensively examined. We conducted a cross-sectional study in a mountainous area of South-...Smoking is a well-known risk factor of reproductive health. However, the effect of paternal smoking on fertility has been less extensively examined. We conducted a cross-sectional study in a mountainous area of South-West China to assess the effect of male smoking on couples＇ fertility. A total of 8200 couples aged 18-49 years in the study area were invited to participate in the study. An in-person interview was performed to collect demographic characteristics of the couples, and husbands＇ life style factors including smoking and drinking habits. Information on time to pregnancy （TTP） was collected retrospectively. Infertility was defined as failure to achieve clinical pregnancy after regular unprotected intercourse for 〉12 months. Logistic regression model was used to estimate the association between male smoking and infertility. A total of 7025 couples were included in the final analysis. After adjusting for potential confounders, the couples were more likely to suffer from infertility if the husbands smoked （adjusted odds ratio [aOR] =1.28, 95% CI： 1.08-1.52） before the first pregnancy. After the analyses were performed according to husbands＇ smoking duration, an increased risk started at a relatively longer smoking duration of 5-10 years （aOR = 1.58, 95% CI. 1.26-1.99） and a stronger association （aOR = 3.34, 95% CI. 2.45-4.56） was observed in the group of 〉10 years. Similar patterns were found for the number of cigarettes smoked per day and the total amount of cigarettes smoked. From our findings, we conclude that male smoking may have an adverse impact on couples＇ infertility.展开更多
Objective To study the effects of pubertal exposure to bisphenol A （BPA） on the reproduction of male mice in adulthood and subsequent generation mice. Methods Male mice aged 21 d were exposed to BPA at a dose of 50 ...Objective To study the effects of pubertal exposure to bisphenol A （BPA） on the reproduction of male mice in adulthood and subsequent generation mice. Methods Male mice aged 21 d were exposed to BPA at a dose of 50 mg/kg per day for 7 d by intraperitoneal injection. Sperm count, sperm deformity rate and testis histology were evaluated 35 d after exposure. Male fertility index and newborns were further observed by mating with the normal female mice. Results The epididymal sperm number was decreased by 20.6% in BPA exposure group compared with the control （P〈O. 01）. Sperm deformity rate in BPA group was increased by 9.65% compared with the control （P〈O.05）. Testis seminiferous tubules were abnormal with sloughing of germ cells; BPA exposure had no significant effects on the fertility of male mice in adulthood; sex ratio of male to female offspring was increased. Conclusion Pubertal exposure to BPA disrupted spermatogenesis in adult mice, and the proportion of male offspring was increased compared with the control.展开更多
As human stem cells with the special pluripotency play important roles in the innovative drug discovery and regenerative medicine,development of extracellular matrix(ECM)mimetics or functional materials that can suppo...As human stem cells with the special pluripotency play important roles in the innovative drug discovery and regenerative medicine,development of extracellular matrix(ECM)mimetics or functional materials that can support stem cell growth and propagation is of high significance.Despite numerous efforts spent,one major limitation restricting the wide applications of stem cells to the clinical translation is the lack of efficient strategies for low cost and large-scale stem cell production under xeno-free culture conditions.Herein,we reported a new strategy with peptides-modified polystyrene-based polymers coated onto the surface of coverslips for the growth and reproduction of human embryonic stem cells(h ESCs).The modified peptides are the active parts of proteins which has been shown to contribute to the pluripotent stem cell attachment or proliferation.The peptides were linked to the glass coverslips coated by the polymer materials via chemical crosslinking,and the composite substrates successfully maintain the longterm growth of HUES-7,H7 and DF699.Our study shows that the coating of polystyrene-derived polymer modified by our developed peptides is a good matrix for long-term growth and reproduction of stem cells.This polystyrene-derived polymer substrate can be produced in large scale and stored for a long time.The most important thing is that it can support the growth of undifferentiated human pluripotent stem cells(h PSCs)for more than ten passages,which could provide a new and relatively easy way to amplify h ESCs in vitro.展开更多
Erythropoietin-producing hepatocellular carcinoma A3(EphA3)is a member of the largest subfamily of tyrosine kinase receptors-Eph receptors.Previous studies have shown that EphA3 is associated with tissue development.R...Erythropoietin-producing hepatocellular carcinoma A3(EphA3)is a member of the largest subfamily of tyrosine kinase receptors-Eph receptors.Previous studies have shown that EphA3 is associated with tissue development.Recently,we have found that the expression of EphA3 is elevated in the hypothalamus of mice with diet-induced obesity(DIO).However,the role of EphA3 in hypothalamic-controlled energy metabolism remains unclear.In the current study,we demonstrated that the deletion of EphA3 in the hypothalamus by CRISPR/Cas9-mediated gene editing promotes obesity in male mice with high-fat diet feeding rather than those with normal chow diet feeding.Moreover,the deletion of hypothalamic EphA3 promotes high-fat DIO by increasing food intake and reducing energy expenditure.Knockdown of EphA3 leads to smaller intracellular vesicles in GT1-7 cells.The current study reveals that hypothalamic EphA3 plays important roles in promoting DIO.展开更多
According to the Annex Technical Regulations for Integrated Curriculum Development(Trial)in Document No.30 of the General Office of the Ministry of Human Resources and Social Security(2012),this paper studies the form...According to the Annex Technical Regulations for Integrated Curriculum Development(Trial)in Document No.30 of the General Office of the Ministry of Human Resources and Social Security(2012),this paper studies the formulation of the curriculum standards for the integration of Chinese medicinal materials production.We focus on the formulation ideas of the curriculum standards for the integration of Chinese medicinal materials production,the formulation process of the curriculum standards for the integration of Chinese medicinal materials production,including the description of typical work tasks,the determination of curriculum objectives,the analysis of study content,the description of referential study tasks,teaching implementation suggestions,assessment and evaluation suggestions,which can provide a reference for the development and research of other related integrated courses.展开更多
Objective: To study the prevalence characteristics of pulmonary nodules and their influencing factors in the health check-up population. Methods: A total of 500 cases of health checkups were selected for the data stud...Objective: To study the prevalence characteristics of pulmonary nodules and their influencing factors in the health check-up population. Methods: A total of 500 cases of health checkups were selected for the data study to analyze the detection and prevalence characteristics of pulmonary nodules. The influencing factors between the pulmonary nodules group and the no pulmonary nodules group were analyzed. Results: A total of 209 cases were detected, of which there were more males than females, and for female patients, the detection rate increased with age (P < 0.05). The distribution of gender, age, smoking, respiratory symptoms, and exposure to kitchen fumes between the two groups was compared (P < 0.05). The multifactorial analysis concluded that risk factors include, respiratory symptoms, older age, exposure to kitchen fumes, and smoking. In addition, patients who exercised more had a lower risk of developing lung nodules. Conclusion: The detection rate of pulmonary nodules was high in the health check-up population and the influencing factors analyzed involved the presence of respiratory symptoms, older age, kitchen fume exposure, and smoking, while the positive influencing factor was increased exercise.展开更多
Objective: To investigate and analyze the risk factors of lung cancer in the population of health checkups. Methods: A total of 500 cases of the health check-up population were selected for data study. Surveyed showed...Objective: To investigate and analyze the risk factors of lung cancer in the population of health checkups. Methods: A total of 500 cases of the health check-up population were selected for data study. Surveyed showed that 19 cases of lung cancer were grouped as the lung cancer group and the remaining 481 cases made up the control group, and the risk factors were analyzed. Results: Among men, there was a significant proportion of individuals aged 60-69 years old, and women aged 30-39 years old. Additionally, individuals aged 60 and above were at increased risk of developing lung cancer. The results of the multifactorial analysis were that the risk factors affecting the detection of lung cancer in healthy people were smoking history, family history of lung cancer, secondhand smoke, history of respiratory diseases, psychosomatic factors, living environment, and kitchen fumes. Conclusion: People over 60 years of age were prone to early lung cancer, followed by individuals aged 30-39 years. It is important to identify the risk factors of lung cancer to strengthen the screening of high-risk groups for early detection and treatment.展开更多
Objective:To statistically determine the effect of salvia polyphenolates combined with doxophylline treatment in patients with chronic pulmonary heart disease in the compensated stage.Methods:From January 2023 to Janu...Objective:To statistically determine the effect of salvia polyphenolates combined with doxophylline treatment in patients with chronic pulmonary heart disease in the compensated stage.Methods:From January 2023 to January 2024,76 patients with chronic pulmonary heart disease in the compensated stage were selected as research subjects.The patients were divided into a research group and a reference group using a randomized numerical table method.The research group was treated with salvia polyphenolates combined with doxophylline,while the reference group received conventional therapy.The treatment effects of the two groups were compared.Results:The patients in the research group,treated with salvia polyphenolates combined with doxophylline,showed maximal ventilation of 73.26±4.83 L/min,left ventricular ejection fraction of 56.14±1.98%,and total effective treatment rate of 94.74%.These results were better than those of the reference group.The differences between the data of the research group and the reference group were statistically significant(P<0.05).Conclusion:For patients with chronic pulmonary heart disease in the compensated stage,treatment with salvia polyphenolates combined with doxophylline significantly improves maximum ventilation and left ventricular ejection fraction,and also results in a higher total effective treatment rate.展开更多
To the Editor:Coronary heart disease(CHD)continues to be a predominant cause of mortality in China even after recommended optimal management.Residual inflammatory risk(RIR)portends increased risk of recurrent cardiova...To the Editor:Coronary heart disease(CHD)continues to be a predominant cause of mortality in China even after recommended optimal management.Residual inflammatory risk(RIR)portends increased risk of recurrent cardiovascular events.As an indicator of pro-inflammatory nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)–interleukin(IL)-1β–IL-6 pathway activation,high-sensitivity C-reactive protein(hsCRP)has demonstrated significant value as a predictor of major adverse cardiovascular and cerebrovascular events(MACCEs).Due to the ethnic differences,East Asian populations exhibited lower hsCRP levels.It was confirmed by our previous single-center study,in which the median of hsCRP was 1 mg/L at 1-month follow-up,and the 1-month hsCRP>1 mg/L showed a better prediction of the risk of MACCE.展开更多
To the Editor:Coronary artery disease(CAD),the most prevalent form of cardiovascular disease(CVD),poses a significant threat to global health,leading to high mortality rates and substantial economic costs.The incidenc...To the Editor:Coronary artery disease(CAD),the most prevalent form of cardiovascular disease(CVD),poses a significant threat to global health,leading to high mortality rates and substantial economic costs.The incidence of CAD in China is 10.2‰and is increasing steadily.[1,2]CAD is a chronic inflammatory disease based on atherosclerosis.One of the most frequently activated inflammatory pathways involved in atherosclerosis is the nucleotide-binding domain and leucine-rich repeat-containing protein 3(NLRP3)inflammasome.[3]Given the crucial impact of NLRP3 on CAD,we aimed to evaluate the correlation between the NLRP3 gene variant and CAD by a case-control association study in a Chinese Han population[Supplementary Figure 1,http://links.lww.com/CM9/C596].展开更多
基金financial support from the National Key R&D Program of China(No.2022YFC3401003)the National Natural Science Foundation of China(Nos.21927808,82073817,22104160)。
文摘Gastric Carcinoma(GC)is a highly fatal malignant tumor with a poor prognosis.Its elevated mortality rates are primarily due to its proclivity for late-stage metastasis.Exploring the metabolic interactions between tumor microenvironment and the systemic bloodstream could help to clearly understand the mechanisms and identify precise biomarkers of tumor growth,proliferation,and metastasis.In this study,an integrative approach that combines plasma metabolomics with mass spectrometry imaging of tumor tissue was developed to investigate the global metabolic landscape of GC tumorigenesis and metastasis.The results showed that the oxidized glutathione to glutathione ratio(GSSH/GSH)became increased in non-distal metastatic GC(M0),which means an accumulation of oxidative stress in tumor tissues.Furthermore,it was found that the peroxidation of polyunsaturated fatty acids,such as 9,10-EpOMe,9-HOTrE,etc.,were accelerated in both plasma and tumor tissues of distal metastatic GC(M1).These changes were further confirmed the potential effect of CYP2E1 and GGT1 in metastatic potential of GC by mass spectrometry imaging(MSI)and immunohistochemistry(IHC).Collectively,our findings reveal the integrated multidimensional metabolomics approach is a clinical useful method to unravel the bloodtumor metabolic crosstalk,illuminate reprogrammed metabolic networks,and provide reliable circulating biomarkers.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDA26050301-02)。
文摘The global rise in animal protein consumption has significantly amplified the demand for fodder.A comprehensive understanding of the diversity and characteristics of existing fodder resources is essential for balanced nutritional fodder production.This study investigates the diversity and composition of fodder plants and identifies key species for cattle in Zhaotong City,Yunnan,China,while documenting indigenous knowledge on their usage and selection criteria.Ethnobotanical surveys were conducted in 19 villages across seven townships with 140 informants.Data were collected through semi-structured interviews,free listing,and participatory observation,and analyzed using Relative Frequency Citation.A total of 125 taxa(including 106 wild and 19 cultivated)were reported.The most cited family is Poaceae(27 taxa,21.43%),followed by Asteraceae(17 taxa,13.49%),Fabaceae(14 taxa,11.11%),Polygonaceae(9 taxa,7.14%)and Lamiaceae(4 taxa,3.17%).The whole plant(66.04%)and herbaceous plants(84.80%)were the most used parts and life forms.The most cited species were Zea mays,Brassica rapa,Solanum tuberosum,Eragrostis nigra,and Artemisia dubia.Usage of diverse fodder resources reflects local wisdom in managing resource availability and achieving balanced nutrition while coping with environmental and climatic risks.Preferences for certain taxonomic groups are due to their quality as premier fodder resources.To promote integrated crop-livestock farming,we suggest further research into highly preferred fodder species,focusing on nutritional assessment,digestibility,meat quality impacts,and potential as antibiotic alternatives.Establishing germplasm and gene banks for fodder resources is also recommended.
基金supported by the National Natural Science Foundation of China(No.82030016,No 82230011 and No.82200533).
文摘Background and Objective Inflammation plays a pivotal role in the progression of coronary artery disease(CAD).High-sensitivity C-reactive protein(hsCRP)serves as a well-established biomarker for assessing cardiovascular inflammation risk.However,the specific intestinal microbiota alteration contributing to increased inflammation remains unclear.Therefore,the present study investigated the correlation between the intestinal microbiota and inflammation in patients with unstable angina(UA).Methods A cohort of 92 patients with UA was recruited for this study.The plasma hsCRP level was measured via a CardioPhase hsCRP assay,fecal samples were collected after admission,and 16S rRNA sequencing was conducted to identify the fecal microbial profile.The participants were classified into two groups according to the median hsCRP level(1.11 mg/L).The composition of the fecal microbiota was compared between patients with hsCRP≥1.11 mg/L and those with hsCRP<1.11 mg/L.Additionally,the correlations between the fecal microbiota and clinical characteristics were analyzed.Results A notable reduction in the relative abundance of Akkermansia was observed in patients with hsCRP≥1.11 mg/L,whereas the diversity of the fecal microbiota was not significantly different between patients with hsCRP≥1.11 mg/L and those with hsCRP<1.11 mg/L.Furthermore,the abundance of Akkermansia was negatively correlated with hsCRP levels.Conclusion This study suggested a significant association between decreased levels of Akkermansia and inflammatory risk in patients with UA.These findings underscore the potential role of the intestinal microbiota in contributing to inflammation in UA patients.Further work is needed on the mechanism by which the microbiota contributes to inflammatory risk.
基金supported by the National Natural Science Foundation of China(Nos.41907325 and 41571470).
文摘The present study was conducted to examine the trophic transfer of potentially toxic elements(PTEs)in a closed arsenic mine.Eight PTEs in a soil-plant-leaf litter-earthworm-top predators(free-range local chicken and wild passerine bird)system were analyzed for nitrogen and carbon stable isotopes,PTE concentrations,bioaccumulation factors(BAFs),and transfer factors(TFs).The PTE concentrations in soils from mining areas were generally higher than a adjacent controlled area,with As and Cd in soils showing the prominent compared to other six PTEs,as seen for the indices of geo-accumulation index(I_(geo)),pollution index(PI)and potential ecological risk index(RI).The relatively high BAF and TF values suggested a distinct biotransfer of PTEs along the soil-plant-leaf litter-earthworm system.BAFs were mostly<1 except in earthworms,indicating that earthworms had a strong capacity to take up these metals.The TFs varied both among PTEs and organism’s species,e.g.,the transfer capacities of As in Pteris vittata and Pteris cretica,Cd in Miscanthus sinensis,and Pb,Cr and Mn in moss were the highest.For local free-range chicken and wild passerine bird,the concentrations of PTEs were higher in gastric contents and feather than in internal tissue(stomach,liver and heart),with lower contents in muscle and egg.Bioaccumulation of PTEs generally decreased from decomposer earthworms,to primary producer plants,to top predator,indicating a potential bio-dilution tendency in higher trophic levels in the terrestrial food chain.
文摘<strong>Objective:</strong> To assess and compare the clinical application value of nursing risk management in the diagnosis and treatment of severe peptic ulcer bleeding. <strong>Methods:</strong> A total of 70 patients with severe peptic ulcer bleeding were selected as the research objects, randomly divided into observation group and control group, 35 cases for each. The nursing risk management and conventional nursing management were implemented for the two groups, respectively. The success rate of hemostasis, average length of stay, cure rate, complication rate, dyspnea rate, nursing dispute rate, SAS score, and SDS score were compared between the two groups. Patients’ satisfaction with nursing quality was also compared. <strong>Results:</strong> After the implementation of two different nursing management methods, all the above-mentioned metrics were better in the observation group than in the control group, and the differences were statistically significant (P < 0.01 or P < 0.05). <strong>Conclusion:</strong> The application of nursing risk management in the nursing intervention process of patients with severe peptic ulcer bleeding can effectively reduce the probability of nursing risk events, increase the cure rate, shorten the patient’s hospital stay, but also improve the patients’ psychological state and increase the patient’s satisfaction with nursing quality, which is worth clinical promotion.
文摘Objective: To explore the application effect of oral glucose combined with short-term intravenous nutrition in preventing postoperative hypoglycemia after painless endoscopic gastric polyp resection, and to provide guidance for better management of patients undergoing such procedures. Methods: A total of 886 patients who underwent painless endoscopic gastric polyp resection in the Department of Gastroenterology, the First Affiliated Hospital of Yangtze University, from January 2020 to December 2021, were selected as the study subjects. According to the random number table method, they were divided into an experimental group and a control group, with 443 cases in each group. Patients in the control group were subjected to routine fasting and water restriction for 8 hours before surgery, and routine fasting for 24 hours after surgery. Short-term intravenous nutrition support was provided through fluid supplementation, and finger blood glucose levels were monitored at 1 hour, 4 hours, and 8 hours after completion of intravenous infusion, or when symptoms such as palpitations and cold sweats occurred. The experimental group received oral administration of 5% glucose solution (500 ml) 2 hours before surgery based on the procedures of the control group. The incidence of preoperative discomfort (hunger, thirst, and fatigue), residual gastric fluid volume, and postoperative hypoglycemia were compared between the two groups. Results: The incidence of postoperative blood glucose Conclusion: Based on the study population, oral glucose combined with short-term intravenous nutrition can effectively prevent the incidence of hypoglycemia in patients undergoing painless endoscopic gastric polyp resection. However, due to the limitations of a single-center study and a small number of cases, its feasibility needs to be further validated with more data from multi-center and large-sample cases.
文摘Background/Aim: Conventional treatment for hypertrophic anal papillae (HAP) has effectiveness and safety concerns. This study aimed to investigate the feasibility, safety, and efficacy of transparent cap-assisted endoscopic hot snare resection for the treatment of HAP. Methods: Endoscopic and clinical data of patients treated with transparent cap-assisted endoscopic hot snare resection for HAP at the Department of Gastroenterology, First Affiliated Hospital of Yangtze University from June 2019 to June 2021, were collected and retrospectively analyzed. Results: A total of 56 patients with HAP were treated with transparent cap-assisted endoscopic hot snare resection, including 53 patients (94.6%) with single hypertrophic anal papillae and 3 patients (5.4%) with multiple HAP;51 patients (83.6%) with basal diameter <5 mm and 10 patients (16.4%) with basal diameter ≥5 mm. The procedures that were performed together with transparent cap-assisted endoscopic hot snare resection for HAP treatment included total colon examination in 56 cases (100%), endoscopic polypectomy in 20 cases (35.7%), endoscopic sclerotherapy for hemorrhoids in 29 cases (51.8%), and endoscopic rubber band ligation for hemorrhoids in 11 cases (19.6%). No patient experienced bleeding during or after HAP with transparent cap-assisted endoscopic hot snare resection. Pain level was evaluated by the visual analog score (VAS) method. 52 patients (92.9%) did not have pain and 4 patients (7.1%) had mild pain 3 days after surgery. At a postoperative follow-up of 2 to 18 months, patient satisfaction with transparent cap-assisted endoscopic hot snare resection for HAP treatment was 100% (56/56). Conclusion: Transparent cap-assisted endoscopic hot snare resection is safe and effective for treating HAP.
文摘BACKGROUND： It has been previously demonstrated that the neural cell microenvironment has the ability to induce differentiation of bone marrow mesenchymal stem cells （BMSCs） into the neural cells. OBJECTIVE： To establish a co-culture system of human BMSCs and neural cells, and to observe effects of this co-culture system on differentiation of human BMSCs into neural cells. DESIGN, TIME AND SETTING： A comparative observation experiment, performed at the Center Labora-tory of the Affiliated Hospital of Medical College Qingdao University from October 2006 to December 2007. MATERIALS： Neural cells were obtained from human fetal brain tissue. BMSCs were harvested from fe-male patients that underwent autonomous stem cell transplantation. METHODS： BMSCs in the co-culture group consisted of BMSCs and third passage neural cells. BMSCs in the control group were solely cultured in vitro. MAIN OUTCOME MEASURES： Morphological changes of BMSCs were observed, and expression of the neuronal specific marker, neuron-specific enolase （NSE）, was analyzed by immunofluorescence staining after 4-5-day co-culture. RESULTS： The number of neural cells in the co-culture group increased and the cells spread on the culture bottle surface. Radial dendrite formed and connected with each other. NSE-immunoreactive cells were also detected. The positive ratio of NSE-positive cells reached （32.7±11.5）%, with morphological characteristics similar to neuronal cells. Human BMSCs did not express NSE in the control group. CONCLUSION： The microenvironment provided by neurons induced differentiation of BMSCs into neu-ronal-like cells.
基金the National Natural Science Foundation of China(Grant Nos.81630046,61627827,61331001 and 91539127)the Science and Technology Planning Project of Guangdong Province,China(Nos.2015B020233016,2014B020215003 and 2014A020215031)+1 种基金the Science and Technology Youth Talent for Special Program of Guangdong,China(Nos.2015TQ01X882)the Distinguished Young Teacher Project in Higher Education of Guangdong,China(No.YQ2015049).
文摘Since changes in mechanical properties of biological tissues are often closely related to pathology,the viscoelastic properties are important physical parameters for medical diagnosis.A photoacoustic(PA)phase-resolved method for noninvasively characterizing the biological tissue viscoelasticity has been proposed by Gao et al.[G.Gao,S.Yang,D.Xing,\Viscoelasticity imaging of biological tissues with phase-resolved photoacoustic measurement,"Opt.Lett.36,3341–3343(2011)].The mathematical relationship between the PA phase delay and the viscosity–elasticity ratio has been theoretically deduced.Moreover,systems of PA viscoelasticity(PAVE)imaging including PAVE microscopy and PAVE endoscopy were developed,and high-PA-phase contrast images re°ecting the tissue viscoelasticity information have been successfully achieved.The PAVE method has been developed in tumor detection,atherosclerosis characterization and related vascular endoscopy.We reviewed the development of the PAVE technique and its applications in biomedical¯elds.It is believed that PAVE imaging is of great potential in both biomedical applications and clinical studies.
文摘Smoking is a well-known risk factor of reproductive health. However, the effect of paternal smoking on fertility has been less extensively examined. We conducted a cross-sectional study in a mountainous area of South-West China to assess the effect of male smoking on couples＇ fertility. A total of 8200 couples aged 18-49 years in the study area were invited to participate in the study. An in-person interview was performed to collect demographic characteristics of the couples, and husbands＇ life style factors including smoking and drinking habits. Information on time to pregnancy （TTP） was collected retrospectively. Infertility was defined as failure to achieve clinical pregnancy after regular unprotected intercourse for 〉12 months. Logistic regression model was used to estimate the association between male smoking and infertility. A total of 7025 couples were included in the final analysis. After adjusting for potential confounders, the couples were more likely to suffer from infertility if the husbands smoked （adjusted odds ratio [aOR] =1.28, 95% CI： 1.08-1.52） before the first pregnancy. After the analyses were performed according to husbands＇ smoking duration, an increased risk started at a relatively longer smoking duration of 5-10 years （aOR = 1.58, 95% CI. 1.26-1.99） and a stronger association （aOR = 3.34, 95% CI. 2.45-4.56） was observed in the group of 〉10 years. Similar patterns were found for the number of cigarettes smoked per day and the total amount of cigarettes smoked. From our findings, we conclude that male smoking may have an adverse impact on couples＇ infertility.
文摘Objective To study the effects of pubertal exposure to bisphenol A （BPA） on the reproduction of male mice in adulthood and subsequent generation mice. Methods Male mice aged 21 d were exposed to BPA at a dose of 50 mg/kg per day for 7 d by intraperitoneal injection. Sperm count, sperm deformity rate and testis histology were evaluated 35 d after exposure. Male fertility index and newborns were further observed by mating with the normal female mice. Results The epididymal sperm number was decreased by 20.6% in BPA exposure group compared with the control （P〈O. 01）. Sperm deformity rate in BPA group was increased by 9.65% compared with the control （P〈O.05）. Testis seminiferous tubules were abnormal with sloughing of germ cells; BPA exposure had no significant effects on the fertility of male mice in adulthood; sex ratio of male to female offspring was increased. Conclusion Pubertal exposure to BPA disrupted spermatogenesis in adult mice, and the proportion of male offspring was increased compared with the control.
基金the National Natural Science Foundation of China(Nos.21877130 and U1801681)The Key Field Research and Development Program of Guangdong Province(No.2019B020235001)+2 种基金Basic and Applied Basic Research Foundation of Guangdong Province(No.2019A1515110451)Science and Technology Program of Guangzhou(No.201803010009)Natural Science Foundation of Guangdong Province(Nos.2018A030313600,2021A1515010357)。
文摘As human stem cells with the special pluripotency play important roles in the innovative drug discovery and regenerative medicine,development of extracellular matrix(ECM)mimetics or functional materials that can support stem cell growth and propagation is of high significance.Despite numerous efforts spent,one major limitation restricting the wide applications of stem cells to the clinical translation is the lack of efficient strategies for low cost and large-scale stem cell production under xeno-free culture conditions.Herein,we reported a new strategy with peptides-modified polystyrene-based polymers coated onto the surface of coverslips for the growth and reproduction of human embryonic stem cells(h ESCs).The modified peptides are the active parts of proteins which has been shown to contribute to the pluripotent stem cell attachment or proliferation.The peptides were linked to the glass coverslips coated by the polymer materials via chemical crosslinking,and the composite substrates successfully maintain the longterm growth of HUES-7,H7 and DF699.Our study shows that the coating of polystyrene-derived polymer modified by our developed peptides is a good matrix for long-term growth and reproduction of stem cells.This polystyrene-derived polymer substrate can be produced in large scale and stored for a long time.The most important thing is that it can support the growth of undifferentiated human pluripotent stem cells(h PSCs)for more than ten passages,which could provide a new and relatively easy way to amplify h ESCs in vitro.
基金supported by the National Natural Science Foundation of China (Grants No.82070872,92049118,and 81570774)the Jiangsu Province's Innovation Personal as well as Innovative and Entrepreneurial Team of Jiangsu Province (Grant No.JSSCTD2021)+1 种基金the National Key Research and Development Program of China (Grant No.2018YFC1003504)the Junior Thousand Talents Program of China,the NJMU startup fund.
文摘Erythropoietin-producing hepatocellular carcinoma A3(EphA3)is a member of the largest subfamily of tyrosine kinase receptors-Eph receptors.Previous studies have shown that EphA3 is associated with tissue development.Recently,we have found that the expression of EphA3 is elevated in the hypothalamus of mice with diet-induced obesity(DIO).However,the role of EphA3 in hypothalamic-controlled energy metabolism remains unclear.In the current study,we demonstrated that the deletion of EphA3 in the hypothalamus by CRISPR/Cas9-mediated gene editing promotes obesity in male mice with high-fat diet feeding rather than those with normal chow diet feeding.Moreover,the deletion of hypothalamic EphA3 promotes high-fat DIO by increasing food intake and reducing energy expenditure.Knockdown of EphA3 leads to smaller intracellular vesicles in GT1-7 cells.The current study reveals that hypothalamic EphA3 plays important roles in promoting DIO.
基金Supported by Scientific Research Fund Project of Yunnan Provincial Department of Education (2023J2034).
文摘According to the Annex Technical Regulations for Integrated Curriculum Development(Trial)in Document No.30 of the General Office of the Ministry of Human Resources and Social Security(2012),this paper studies the formulation of the curriculum standards for the integration of Chinese medicinal materials production.We focus on the formulation ideas of the curriculum standards for the integration of Chinese medicinal materials production,the formulation process of the curriculum standards for the integration of Chinese medicinal materials production,including the description of typical work tasks,the determination of curriculum objectives,the analysis of study content,the description of referential study tasks,teaching implementation suggestions,assessment and evaluation suggestions,which can provide a reference for the development and research of other related integrated courses.
文摘Objective: To study the prevalence characteristics of pulmonary nodules and their influencing factors in the health check-up population. Methods: A total of 500 cases of health checkups were selected for the data study to analyze the detection and prevalence characteristics of pulmonary nodules. The influencing factors between the pulmonary nodules group and the no pulmonary nodules group were analyzed. Results: A total of 209 cases were detected, of which there were more males than females, and for female patients, the detection rate increased with age (P < 0.05). The distribution of gender, age, smoking, respiratory symptoms, and exposure to kitchen fumes between the two groups was compared (P < 0.05). The multifactorial analysis concluded that risk factors include, respiratory symptoms, older age, exposure to kitchen fumes, and smoking. In addition, patients who exercised more had a lower risk of developing lung nodules. Conclusion: The detection rate of pulmonary nodules was high in the health check-up population and the influencing factors analyzed involved the presence of respiratory symptoms, older age, kitchen fume exposure, and smoking, while the positive influencing factor was increased exercise.
文摘Objective: To investigate and analyze the risk factors of lung cancer in the population of health checkups. Methods: A total of 500 cases of the health check-up population were selected for data study. Surveyed showed that 19 cases of lung cancer were grouped as the lung cancer group and the remaining 481 cases made up the control group, and the risk factors were analyzed. Results: Among men, there was a significant proportion of individuals aged 60-69 years old, and women aged 30-39 years old. Additionally, individuals aged 60 and above were at increased risk of developing lung cancer. The results of the multifactorial analysis were that the risk factors affecting the detection of lung cancer in healthy people were smoking history, family history of lung cancer, secondhand smoke, history of respiratory diseases, psychosomatic factors, living environment, and kitchen fumes. Conclusion: People over 60 years of age were prone to early lung cancer, followed by individuals aged 30-39 years. It is important to identify the risk factors of lung cancer to strengthen the screening of high-risk groups for early detection and treatment.
文摘Objective:To statistically determine the effect of salvia polyphenolates combined with doxophylline treatment in patients with chronic pulmonary heart disease in the compensated stage.Methods:From January 2023 to January 2024,76 patients with chronic pulmonary heart disease in the compensated stage were selected as research subjects.The patients were divided into a research group and a reference group using a randomized numerical table method.The research group was treated with salvia polyphenolates combined with doxophylline,while the reference group received conventional therapy.The treatment effects of the two groups were compared.Results:The patients in the research group,treated with salvia polyphenolates combined with doxophylline,showed maximal ventilation of 73.26±4.83 L/min,left ventricular ejection fraction of 56.14±1.98%,and total effective treatment rate of 94.74%.These results were better than those of the reference group.The differences between the data of the research group and the reference group were statistically significant(P<0.05).Conclusion:For patients with chronic pulmonary heart disease in the compensated stage,treatment with salvia polyphenolates combined with doxophylline significantly improves maximum ventilation and left ventricular ejection fraction,and also results in a higher total effective treatment rate.
基金supported by grants from the Noncommunicable Chronic Diseases—National Science and Technology Major Project(No.2025ZD0548400)the National Key Research and Development Program(No.2022YFC2503501)+3 种基金Chinese Society of Cardiology’s Foundation(No.CSCF2023A04)Hubei Technology Innovation Project(No.2024BCB046)Key Research and Development Program of Wuhan(No.2024020702030092)to Xiang ChengThe National Heart,Lung,and Blood Institute(Nos.1R01HL134892 and 1R01HL163099-01),the RRM Charitable Fund,and the Simard Fund to Peter Libby.
文摘To the Editor:Coronary heart disease(CHD)continues to be a predominant cause of mortality in China even after recommended optimal management.Residual inflammatory risk(RIR)portends increased risk of recurrent cardiovascular events.As an indicator of pro-inflammatory nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)–interleukin(IL)-1β–IL-6 pathway activation,high-sensitivity C-reactive protein(hsCRP)has demonstrated significant value as a predictor of major adverse cardiovascular and cerebrovascular events(MACCEs).Due to the ethnic differences,East Asian populations exhibited lower hsCRP levels.It was confirmed by our previous single-center study,in which the median of hsCRP was 1 mg/L at 1-month follow-up,and the 1-month hsCRP>1 mg/L showed a better prediction of the risk of MACCE.
基金This work was supported by grants from the National Key Research and Development Program(No.2022YFC2503501)Chinese Society of Cardiology’s Foundation(No.CSCF2023A04)+2 种基金Hubei Technology Innovation Project(No.2024BCB046)Key Research and Development Program of Wuhan(No.2024020702030092)the National Natural Science Foundation of China(No.82200319).
文摘To the Editor:Coronary artery disease(CAD),the most prevalent form of cardiovascular disease(CVD),poses a significant threat to global health,leading to high mortality rates and substantial economic costs.The incidence of CAD in China is 10.2‰and is increasing steadily.[1,2]CAD is a chronic inflammatory disease based on atherosclerosis.One of the most frequently activated inflammatory pathways involved in atherosclerosis is the nucleotide-binding domain and leucine-rich repeat-containing protein 3(NLRP3)inflammasome.[3]Given the crucial impact of NLRP3 on CAD,we aimed to evaluate the correlation between the NLRP3 gene variant and CAD by a case-control association study in a Chinese Han population[Supplementary Figure 1,http://links.lww.com/CM9/C596].
