Phosphorus(P) limitation in the coming decades calls for the utilization of alternative fertilizers in agriculture. Struvite is a promising P source, but its potential role as a fertilizer is dependent on different ph...Phosphorus(P) limitation in the coming decades calls for the utilization of alternative fertilizers in agriculture. Struvite is a promising P source, but its potential role as a fertilizer is dependent on different physical, chemical, and biological properties, which are very heterogeneous in soil, complicating the prediction of the best soil conditions for its application. Here, we evaluated the solubility of struvite in soil, its redistribution into P fractions, and its potential abiotic and biotic drivers in 62 globally distributed soils with contrasting properties through an incubation assay. We found that after 40 d, about 35% of struvite P was redistributed into soil fractions more accessible to plants and microbes. Phosphorus redistribution from struvite was driven by a complex suite of soil physical, chemical, and microbial properties as well as environmental factors that varied across soils. Soil texture played a critical role in determining the redistribution of P in struvite-amended soils in soluble(H2O extraction), labile(NaHCO3 extraction), and moderately labile(NaOH extraction) fractions.In addition, the soil solution cation concentration was one of the most important drivers of available struvite-derived P fractions. The great importance of texture and cations in determining struvite-derived P fractions in soil was contrasted with the relatively minor role of pH. At the microbial level, the number of bacterial operational taxonomic units(OTUs) from the unfertilized soils that correlated with struvite-derived P fractions was higher than that of fungi. The number of OTUs that correlated with the struvite-derived soluble P fraction was dominated by fungi, whereas the number of OTUs that correlated with the struvite-derived labile P fraction was dominated by bacteria. Overall, this study provided a predictive framework for the potential use of struvite as a P fertilizer in contrasting soils.展开更多
Halotolerant plant growth-promoting bacteria(PGPB)have great potential for alleviating salinity stress in crops.However,the current methods used with these bacteria are typically based on one-time inoculations,includi...Halotolerant plant growth-promoting bacteria(PGPB)have great potential for alleviating salinity stress in crops.However,the current methods used with these bacteria are typically based on one-time inoculations,including soil basal application,seed dressing and plant infestation,all of which make it difficult to guarantee the desired plant effects.Here,we investigated the effects of seven halotolerant PGPB individually applied through a drip irrigation system in small quantities and at high frequency during the plant's growth period on the soil physicochemical properties,plant agronomic performance and bacterial community in saline soil.Our findings revealed that drip irrigation with halotolerant PGPB notably decreased the soil pH and electrical conductivity while increasing the yield and fruit quality of jujube plants.Specifically,the Bacillus licheniformis(BL)and Bacillus mucilaginous(BM)treatments outperformed the control(no PGPB irrigation)by increasing the yield and vitamin C(VC)content by 23%and 22%,respectively.Additionally,the presence of halotolerant PGPB enriched the diversity of the bacterial community in the jujube rhizosphere and increased the relative abundance of beneficial bacterial groups at both the phylum(e.g.,Cyanobacteria and Nitrospirota)and genus(e.g.,Psychrobacter,Flavobacterium,and Steroidobacter)levels.Bacterial interactions,represented by co-occurrence networks,were more complex in the treatments involving PGPB irrigation,contributing to the transformation of the network keystones involved in soil nutrient cycling.Applications of BL,Bacillus cereus(BC),and BM reduced the soil salinity and increased the soil available nutrient contents and plant antioxidant enzyme activities,alleviating salinity stress and resulting in increases in crop yield and quality.This study highlights the feasibility and efficiency of applying halotolerant PGPB via drip irrigation in saline soil environments,thereby enhancing crop performance under salt stress.展开更多
The impact of forest microhabitats on physiochemical properties of the soil and that of microbial communities on tropical soils remain poorly understood.To elucidate the effect of tropical forest stand on leaf litter ...The impact of forest microhabitats on physiochemical properties of the soil and that of microbial communities on tropical soils remain poorly understood.To elucidate the effect of tropical forest stand on leaf litter and soil microbial communities,we studied enzyme activities,microbial biomass,and diversity in three distinct microhabitats in terms of plant richness,diameter at breast height(DBH),and physiochemical properties of soil and litter,each associated with a different Vanilla sp.In the soil,positive correlations were found between electrical conductivity(EC)and total organic carbon(TOC)with phosphatase activity,and between nitrogen(N)and water-soluble carbon(WSC)content with urease activity(UA).In the litter,the water content was positively correlated with bacterial and fungal biomass,and N and WSC contents were positively correlated with fungal biomass.Positive correlations were found between plant richness and UA in the soil,plant richness and fungal biomass in the soil and litter,and DBH and fungal biomass in the litter.Amplicon sequencing revealed differences between microhabitats in the relative abundance of some fungal and bacterial taxa and in the bacterial community composition of both litter and soil.Bacterial richness and diversity were different between microhabitats,and,in litter samples,they were negatively correlated with DBH and plant richness,respectively.By contrast,none of the soil and litter physiochemical properties were significantly correlated with microbial diversity.Our results show that significant shifts in enzyme activity,microbial biomass,and diversity in the microhabitats were driven by key abiotic and biotic factors depending on the soil or litter sample type.展开更多
基金the financial support by the Fundacion General CSIC, Spain (Programa ComFuturo)the project PID2020114942RB-I00 funded by MCIN/AEI//10.13039/5011000 11033+3 种基金supported by a project from the Spanish Ministry of Science and Innovation (No. PID2020-115813RA-I00)a project of the Fondo Europeo de Desarrollo Regional (FEDER)the Consejería de Transformación Económica, Industria, Conocimiento y Universidades of the Junta de Andalucía (FEDER Andalucía 2014-2020 Objetivo temático “01— Refuerzo de la investigación, el desarrollo tecnológico y la innovación”, ANDABIOMA, No. P20_00879)supported by a postdoctoral scholarship as part of the FCT-funded project “Soil Ecosystems in the XXI Century: Drivers, Conservation and Future Scenarios” (No. FCT-PTDC/BIACBI/2340/2020) led by IPVC, Portugal。
文摘Phosphorus(P) limitation in the coming decades calls for the utilization of alternative fertilizers in agriculture. Struvite is a promising P source, but its potential role as a fertilizer is dependent on different physical, chemical, and biological properties, which are very heterogeneous in soil, complicating the prediction of the best soil conditions for its application. Here, we evaluated the solubility of struvite in soil, its redistribution into P fractions, and its potential abiotic and biotic drivers in 62 globally distributed soils with contrasting properties through an incubation assay. We found that after 40 d, about 35% of struvite P was redistributed into soil fractions more accessible to plants and microbes. Phosphorus redistribution from struvite was driven by a complex suite of soil physical, chemical, and microbial properties as well as environmental factors that varied across soils. Soil texture played a critical role in determining the redistribution of P in struvite-amended soils in soluble(H2O extraction), labile(NaHCO3 extraction), and moderately labile(NaOH extraction) fractions.In addition, the soil solution cation concentration was one of the most important drivers of available struvite-derived P fractions. The great importance of texture and cations in determining struvite-derived P fractions in soil was contrasted with the relatively minor role of pH. At the microbial level, the number of bacterial operational taxonomic units(OTUs) from the unfertilized soils that correlated with struvite-derived P fractions was higher than that of fungi. The number of OTUs that correlated with the struvite-derived soluble P fraction was dominated by fungi, whereas the number of OTUs that correlated with the struvite-derived labile P fraction was dominated by bacteria. Overall, this study provided a predictive framework for the potential use of struvite as a P fertilizer in contrasting soils.
基金funded by the National Natural Science Foundation of China(52339004)the Pinduoduo–China Agricultural University Research Fund(PC2023A02002)the Bintuan Science and Technology Program(2023AB071)。
文摘Halotolerant plant growth-promoting bacteria(PGPB)have great potential for alleviating salinity stress in crops.However,the current methods used with these bacteria are typically based on one-time inoculations,including soil basal application,seed dressing and plant infestation,all of which make it difficult to guarantee the desired plant effects.Here,we investigated the effects of seven halotolerant PGPB individually applied through a drip irrigation system in small quantities and at high frequency during the plant's growth period on the soil physicochemical properties,plant agronomic performance and bacterial community in saline soil.Our findings revealed that drip irrigation with halotolerant PGPB notably decreased the soil pH and electrical conductivity while increasing the yield and fruit quality of jujube plants.Specifically,the Bacillus licheniformis(BL)and Bacillus mucilaginous(BM)treatments outperformed the control(no PGPB irrigation)by increasing the yield and vitamin C(VC)content by 23%and 22%,respectively.Additionally,the presence of halotolerant PGPB enriched the diversity of the bacterial community in the jujube rhizosphere and increased the relative abundance of beneficial bacterial groups at both the phylum(e.g.,Cyanobacteria and Nitrospirota)and genus(e.g.,Psychrobacter,Flavobacterium,and Steroidobacter)levels.Bacterial interactions,represented by co-occurrence networks,were more complex in the treatments involving PGPB irrigation,contributing to the transformation of the network keystones involved in soil nutrient cycling.Applications of BL,Bacillus cereus(BC),and BM reduced the soil salinity and increased the soil available nutrient contents and plant antioxidant enzyme activities,alleviating salinity stress and resulting in increases in crop yield and quality.This study highlights the feasibility and efficiency of applying halotolerant PGPB via drip irrigation in saline soil environments,thereby enhancing crop performance under salt stress.
基金the Spanish Ministry of Science and the Spanish Research Council(CSIC)call“I-COOP Suelos y Legumbres 2016”for the funded project(2016SU0013)supported by the Universidad Nacional,Costa Rica(grant number SIA-0249-18)。
文摘The impact of forest microhabitats on physiochemical properties of the soil and that of microbial communities on tropical soils remain poorly understood.To elucidate the effect of tropical forest stand on leaf litter and soil microbial communities,we studied enzyme activities,microbial biomass,and diversity in three distinct microhabitats in terms of plant richness,diameter at breast height(DBH),and physiochemical properties of soil and litter,each associated with a different Vanilla sp.In the soil,positive correlations were found between electrical conductivity(EC)and total organic carbon(TOC)with phosphatase activity,and between nitrogen(N)and water-soluble carbon(WSC)content with urease activity(UA).In the litter,the water content was positively correlated with bacterial and fungal biomass,and N and WSC contents were positively correlated with fungal biomass.Positive correlations were found between plant richness and UA in the soil,plant richness and fungal biomass in the soil and litter,and DBH and fungal biomass in the litter.Amplicon sequencing revealed differences between microhabitats in the relative abundance of some fungal and bacterial taxa and in the bacterial community composition of both litter and soil.Bacterial richness and diversity were different between microhabitats,and,in litter samples,they were negatively correlated with DBH and plant richness,respectively.By contrast,none of the soil and litter physiochemical properties were significantly correlated with microbial diversity.Our results show that significant shifts in enzyme activity,microbial biomass,and diversity in the microhabitats were driven by key abiotic and biotic factors depending on the soil or litter sample type.
