Two-photon imaging has attracted increasing attention owing to its deep tissue imaging capabilities.Therefore,many fluorophores have been developed to satisfy its requirements.However,long-wavelength emission fluoroph...Two-photon imaging has attracted increasing attention owing to its deep tissue imaging capabilities.Therefore,many fluorophores have been developed to satisfy its requirements.However,long-wavelength emission fluorophores with an optically tunable group are rarely developed.In this study,two longwavelength emission fluorophores with an optically tunable amino group were successfully developed by introducing strong electron acceptor and large conjugated group to the TPQL dye.TPCO_(2)displayed a bright red emission(λem=638 nm,Φ=0.15)together with high two-photon action cross section and good water solubility,which enabled higher signal-to-background ratios and deep tissue imaging.The proof-of-concept probe(TPCO-NO_(2))was successfully applied to the high signal-to-background ratio imaging of nitroreductase in liver fibrosis,further realizing diagnosis of the degree of hypoxia during liver fibrosis.展开更多
Rhodamine dyes have been widely employed in biological imaging and sensing. However, it is always a challenge to design rhodamine derivatives with huge Stokes shift to address the draconian requirements of single-exci...Rhodamine dyes have been widely employed in biological imaging and sensing. However, it is always a challenge to design rhodamine derivatives with huge Stokes shift to address the draconian requirements of single-excitation multicolor imaging. In this work, we described a generally strategy to enhance the Stokes shift of rhodamine dyes by completely breaking their electronic symmetry. As a result, the Stokes shift of novel rhodamine dye DQF-RB-Cl is up to 205 nm in PBS, which is the largest in all the reported rhodamine derivatives. In addition, we successfully realized the single excitation trichromatic imaging of mitochondria, lysosomes and cell membranes by combining DQF-RB-Cl with commercial lysosomal targeting probe Lyso-Tracker Green and membrane targeting dye Dil. This is the organic synthetic dyes for SLE-trichromatic imaging in cells for the first time. These results demonstrate the potential of our design as a useful strategy to develop huge Stokes shift fluorophore for bioimaging.展开更多
Fluorescent probes based on rhodamine skeleton are extensively used in biological imaging.However,the construction of ratiometric fluorescent probes based on the rhodamine skeleton without introducing additional fluor...Fluorescent probes based on rhodamine skeleton are extensively used in biological imaging.However,the construction of ratiometric fluorescent probes based on the rhodamine skeleton without introducing additional fluorophores is still challenging.Herein,we propose an effective method to construct a rhodamine-based ratiometric fluorescent probe through the regulation of electron cloud density.A ratiometric fluorescent probe RDQF-RB-NTR was successfully constructed for the detection of nitroreductase(NTR).RDQF-RB-NTR exhibits good sensitivity,high selectivity,and ratiometric response to NTR.Cell imaging experiments showed that RDQF-RB-NTR can rapidly and accurately detect the fluctuation of NTR in cells and difference of NTR levels between normal cells and cancer cells.In addition,RDQF-RB-NTR was successfully applied to the imaging of NTR in liver tissue slices,and we found that the level of NTR was upregulated in liver cirrhosis.展开更多
In this article,the robust control problem of discrete-time Markov jump systems(MJSs)with actuator saturation is investigated via the passivity theory.Under the assumption of mode synchronization between the system an...In this article,the robust control problem of discrete-time Markov jump systems(MJSs)with actuator saturation is investigated via the passivity theory.Under the assumption of mode synchronization between the system and the controller,sufficient conditions are established to guarantee the system to be mean-square stable and stochastically passive in the domain of attraction via the saturation-dependent Lyapunov function approach and the linear matrix inequality(LMI)technique.The coupling between the system variables is decoupled,which greatly facilitates the design of the synchronization controller.,Moreover,the estimation of the domain of attraction for the considered MJSs is accomplished through the solution of an optimization problem(OP).By degenerating the mode-dependent controller into its mode-independent counterpart,we derive sufficient conditions to ensure system robustness under the mode-independent control strategy,and then systematically summarize these conditions.Finally,the effectiveness of the proposed integrated design methodology is validated through numerical simulations.展开更多
Excessive acute alcohol intake can overload hepatic ethanol metabolic pathways,inducing oxidative stress responses,lipid metabolism disorders,and hepatocyte apoptotic cascades.Current clinical interventions are mostly...Excessive acute alcohol intake can overload hepatic ethanol metabolic pathways,inducing oxidative stress responses,lipid metabolism disorders,and hepatocyte apoptotic cascades.Current clinical interventions are mostly symptomatic supportive care,and there remains a lack of effective strategies to directly convert ethanol into non-toxic metabolites in the digestive tract.In this study,Heyndrickxia(H.)coagulans JF1 was isolated from samples of chicken intestinal contents,and its ethanol-degrading capacity and probiotic functional characteristics were systematically evaluated.Notably,H.coagulans JF1 exhibited excellent ethanol-degrading capacity,ethanol tolerance,and gastrointestinal environment tolerance.This strain showed good adhesion to Caco-2 cells,along with remarkable hydrophobicity,auto-aggregation,and co-aggregation abilities.Antioxidant activity assays demonstrated that JF1 possesses strong scavenging capacity against various free radicals,with cell lysates displaying higher antioxidant levels.Antibacterial activity tests indicated that JF1 can inhibit five species of foodborne pathogens,is susceptible to ten antibiotics,and exhibits no hemolytic activity.In cellular experiments,intervention with JF1 regulated the expression of genes related to oxidative stress,lipid metabolism,immunity,autophagy,and apoptosis in ethanol-induced HepG2 cells.Whole-genome sequencing of JF1 revealed its genetic composition and relevant metabolic pathways,providing a genetic basis for its functional characteristics.Through multi-dimensional phenotypic analysis and genomic characterization,this study offers important theoretical and experimental support for the development of H.coagulans JF1 as a functional probiotic that promotes ethanol metabolism in the body.展开更多
基金supported by the National Natural Science Foundation of China(Nos.22074036,22004033,21877029)Special Funds for the Construction of Innovative Provinces in Hunan Province(No.2019RS1031)。
文摘Two-photon imaging has attracted increasing attention owing to its deep tissue imaging capabilities.Therefore,many fluorophores have been developed to satisfy its requirements.However,long-wavelength emission fluorophores with an optically tunable group are rarely developed.In this study,two longwavelength emission fluorophores with an optically tunable amino group were successfully developed by introducing strong electron acceptor and large conjugated group to the TPQL dye.TPCO_(2)displayed a bright red emission(λem=638 nm,Φ=0.15)together with high two-photon action cross section and good water solubility,which enabled higher signal-to-background ratios and deep tissue imaging.The proof-of-concept probe(TPCO-NO_(2))was successfully applied to the high signal-to-background ratio imaging of nitroreductase in liver fibrosis,further realizing diagnosis of the degree of hypoxia during liver fibrosis.
基金supported by the National Natural Science Foundation of China (Nos. 22074036, 22004033, 21877029)the National Postdoctoral Program for Innovative Talents (No. BX20190110)the China Postdoctoral Science Foundation (No. 2019M662758)。
文摘Rhodamine dyes have been widely employed in biological imaging and sensing. However, it is always a challenge to design rhodamine derivatives with huge Stokes shift to address the draconian requirements of single-excitation multicolor imaging. In this work, we described a generally strategy to enhance the Stokes shift of rhodamine dyes by completely breaking their electronic symmetry. As a result, the Stokes shift of novel rhodamine dye DQF-RB-Cl is up to 205 nm in PBS, which is the largest in all the reported rhodamine derivatives. In addition, we successfully realized the single excitation trichromatic imaging of mitochondria, lysosomes and cell membranes by combining DQF-RB-Cl with commercial lysosomal targeting probe Lyso-Tracker Green and membrane targeting dye Dil. This is the organic synthetic dyes for SLE-trichromatic imaging in cells for the first time. These results demonstrate the potential of our design as a useful strategy to develop huge Stokes shift fluorophore for bioimaging.
基金supported by the National Natural Science Foundation of China(Project:22074036 and 22004033).
文摘Fluorescent probes based on rhodamine skeleton are extensively used in biological imaging.However,the construction of ratiometric fluorescent probes based on the rhodamine skeleton without introducing additional fluorophores is still challenging.Herein,we propose an effective method to construct a rhodamine-based ratiometric fluorescent probe through the regulation of electron cloud density.A ratiometric fluorescent probe RDQF-RB-NTR was successfully constructed for the detection of nitroreductase(NTR).RDQF-RB-NTR exhibits good sensitivity,high selectivity,and ratiometric response to NTR.Cell imaging experiments showed that RDQF-RB-NTR can rapidly and accurately detect the fluctuation of NTR in cells and difference of NTR levels between normal cells and cancer cells.In addition,RDQF-RB-NTR was successfully applied to the imaging of NTR in liver tissue slices,and we found that the level of NTR was upregulated in liver cirrhosis.
基金Project supported by the National Natural Science Foundation of China(No.62303421)the Zhejiang Provincial Natural ScienceFoundation of China(No.LQ23F030018)。
文摘In this article,the robust control problem of discrete-time Markov jump systems(MJSs)with actuator saturation is investigated via the passivity theory.Under the assumption of mode synchronization between the system and the controller,sufficient conditions are established to guarantee the system to be mean-square stable and stochastically passive in the domain of attraction via the saturation-dependent Lyapunov function approach and the linear matrix inequality(LMI)technique.The coupling between the system variables is decoupled,which greatly facilitates the design of the synchronization controller.,Moreover,the estimation of the domain of attraction for the considered MJSs is accomplished through the solution of an optimization problem(OP).By degenerating the mode-dependent controller into its mode-independent counterpart,we derive sufficient conditions to ensure system robustness under the mode-independent control strategy,and then systematically summarize these conditions.Finally,the effectiveness of the proposed integrated design methodology is validated through numerical simulations.
基金supported by the National Natural Science Foundation of China(No.32572590)the Priority Academic Program Development of Jiangsu Higher Education Institution(PAPD),the Cross-Innovation Open Project of Food Flavor and Health,Beijing Technology&Business University.
文摘Excessive acute alcohol intake can overload hepatic ethanol metabolic pathways,inducing oxidative stress responses,lipid metabolism disorders,and hepatocyte apoptotic cascades.Current clinical interventions are mostly symptomatic supportive care,and there remains a lack of effective strategies to directly convert ethanol into non-toxic metabolites in the digestive tract.In this study,Heyndrickxia(H.)coagulans JF1 was isolated from samples of chicken intestinal contents,and its ethanol-degrading capacity and probiotic functional characteristics were systematically evaluated.Notably,H.coagulans JF1 exhibited excellent ethanol-degrading capacity,ethanol tolerance,and gastrointestinal environment tolerance.This strain showed good adhesion to Caco-2 cells,along with remarkable hydrophobicity,auto-aggregation,and co-aggregation abilities.Antioxidant activity assays demonstrated that JF1 possesses strong scavenging capacity against various free radicals,with cell lysates displaying higher antioxidant levels.Antibacterial activity tests indicated that JF1 can inhibit five species of foodborne pathogens,is susceptible to ten antibiotics,and exhibits no hemolytic activity.In cellular experiments,intervention with JF1 regulated the expression of genes related to oxidative stress,lipid metabolism,immunity,autophagy,and apoptosis in ethanol-induced HepG2 cells.Whole-genome sequencing of JF1 revealed its genetic composition and relevant metabolic pathways,providing a genetic basis for its functional characteristics.Through multi-dimensional phenotypic analysis and genomic characterization,this study offers important theoretical and experimental support for the development of H.coagulans JF1 as a functional probiotic that promotes ethanol metabolism in the body.
