Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features.Our exploration of how the genomes orchestrate the formation and maintenance of each cell,and co...Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features.Our exploration of how the genomes orchestrate the formation and maintenance of each cell,and control the cellular phenotypes of various organismsis,is both captivating and intricate.Since the inception of the first single-cell RNA technology,technologies related to single-cell sequencing have experienced rapid advancements in recent years.These technologies have expanded horizontally to include single-cell genome,epigenome,proteome,and metabolome,while vertically,they have progressed to integrate multiple omics data and incorporate additional information such as spatial scRNA-seq and CRISPR screening.Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases,including cancers.Here,we comprehensively summarize recent advances in single-cell omics technologies,with a specific focus on the methodology section.This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.展开更多
基金supported by the National Natural Science Foundation of China(32130020,32025009,82030099,30700397,31970638,61572361,81973701,U23A20513,32222026,82373446)the National Key Research and Development Program of China(2021YFF1201200,2021YFF1200900,2022YFA1106000)+10 种基金the Shanghai Municipal Science and Technology Major Project(2017SHZDZX01)the Fundamental Research Funds for the Central Universities(20002150110,22120230292)Beihang University&Capital Medical University Plan(BHME-201904)the Special Fund of the Pediatric Medical Coordinated Development Center of Beijing Hospitals Authority(XTCX201809)the Cooperative Research Fund of the Affiliated Wuhu Hospital of East China Normal University(40500-20104-222400)the Fundamental Research Funds for the Central Universities(226-2024-00001)the Shanghai Municipal Science and Technology Commission“Science and Technology Innovation Action Plan”technical standard project(21DZ2201700)the Shanghai Municipal Science and Technology Commission“Science and Technology Innovation Action Plan”natural science foundation project(23ZR1435800)the Shanghai Natural Science Foundation Program(17ZR1449400)the Shanghai Artificial Intelligence Technology Standard Project(19DZ2200900)the Open Research Fund of Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE,ECNU,Key Laboratory of MEA,Ministry of Education,ECNU.
文摘Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features.Our exploration of how the genomes orchestrate the formation and maintenance of each cell,and control the cellular phenotypes of various organismsis,is both captivating and intricate.Since the inception of the first single-cell RNA technology,technologies related to single-cell sequencing have experienced rapid advancements in recent years.These technologies have expanded horizontally to include single-cell genome,epigenome,proteome,and metabolome,while vertically,they have progressed to integrate multiple omics data and incorporate additional information such as spatial scRNA-seq and CRISPR screening.Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases,including cancers.Here,we comprehensively summarize recent advances in single-cell omics technologies,with a specific focus on the methodology section.This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.
