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Accurate detection method of pig's temperature based on non-point source thermal infrared image 被引量:4
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作者 Zaiqin Zhang Hao Wang +6 位作者 Tonghai Liu Yueqiang Wang Hang Zhang feiyan yuan Xue Yang Shunlai Xu Yuhuan Meng 《CAAI Transactions on Intelligence Technology》 EI 2021年第3期312-323,共12页
Body temperature measurement is a very important task in the sow breeding process.The authors used an infrared camera to detect the temperature of the body surface of the sows,relying on calculating the average of the... Body temperature measurement is a very important task in the sow breeding process.The authors used an infrared camera to detect the temperature of the body surface of the sows,relying on calculating the average of the infrared image temperature in the ear root region.Based on the grayscale value of the target image of the infrared image and the corresponding temperature value of 180 infrared images,a G-T(Gray-Temperature)model was established by linear least squares method,which achieved temperature inversion of each pixel of the target pig.For the different growth stages and different breeds of sows,the R-square of the all established models is greater than 0.95.The average relative error of the model inversion of the body temperature was only 0.076977%.This means that the body temperature of the sows could be detected without relying on the software.Based on the G-T model,the authors design a kind of sow's ear root recognition and body surface temperature detection algorithm for different sow population scenarios. 展开更多
关键词 IMAGE METHOD BREEDING
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枯草芽孢杆菌中一种新型双向启动子的功能鉴定 被引量:1
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作者 柴昊男 张会图 +2 位作者 袁飞燕 刘焕 路福平 《生物工程学报》 CAS CSCD 北大核心 2019年第7期1326-1334,共9页
本研究旨在通过转录组分析预测的方法,由地衣芽孢杆菌中筛选获得一种新型双向启动子,鉴定其启动强度。以已知强组成型启动子pShuttle-09为对照,检测其对克劳氏芽孢杆菌碱性蛋白酶基因的表达活性。成功构建了3种重组碱性蛋白酶表达载体... 本研究旨在通过转录组分析预测的方法,由地衣芽孢杆菌中筛选获得一种新型双向启动子,鉴定其启动强度。以已知强组成型启动子pShuttle-09为对照,检测其对克劳氏芽孢杆菌碱性蛋白酶基因的表达活性。成功构建了3种重组碱性蛋白酶表达载体及对应的工程菌株。在新型启动子pLA和其反向启动子pLB调控转录下,克劳氏芽孢杆菌碱性蛋白酶表达活性达到164U/mL和111U/mL。结果表明,pLA的启动强度明显高于pShuttle-09和pLB,pLA启动子与pLB启动子均可表达碱性蛋白酶。从而为枯草芽孢杆菌表达系统中异源基因的表达提供一个新的方向,也为原核生物中共同表达两种基因提供了新的思路。 展开更多
关键词 双向启动子 芽孢杆菌 碱性蛋白酶
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