Age-related macular degeneration(AMD)represents a predominant cause of blindness among older adults,with limited therapeutic options currently available.Oxidative stress,inflammation,and retinal pigment epithelium inj...Age-related macular degeneration(AMD)represents a predominant cause of blindness among older adults,with limited therapeutic options currently available.Oxidative stress,inflammation,and retinal pigment epithelium injury are recognized as key contributors to the pathogenesis of AMD.Regulated cell death plays a pivotal role in mediating cellular responses to stress,maintaining tissue homeostasis,and contributing to disease progression.Recent research has elucidated several regulated cell death pathwaysdsuch as apoptosis,ferroptosis,pyroptosis,necroptosis,and autophagydthat may contribute to the progression of AMD owing to cell death in the retinal pigment epithelium.These discoveries open new avenues for therapeutic interventions in patients with AMD.In this review,we provide a comprehensive summary and analysis of the latest advancements regarding the relationship between regulated cell death and AMD.Moreover,we examined the therapeutic potential of targeting regulated cell death pathways for the treatment and prevention of AMD,highlighting their roles as promising targets for future therapeutic strategies.展开更多
Ganoderma(lingzhi)is a famous herbal medicine and edible supplement in oriental countries for a long history.In this study,a simple micellar electrokinetic chromatography(MEKC)method was established for the analysis o...Ganoderma(lingzhi)is a famous herbal medicine and edible supplement in oriental countries for a long history.In this study,a simple micellar electrokinetic chromatography(MEKC)method was established for the analysis of nucleosides and bases,the major bioactive components in Ganoderma for the first time.By optimizing the borate concentration,the sodium dodecyl sulfate(SDS)concentration and the pH value of running buffer,10 nucleosides and bases achieved an ideal separation.In real sample analysis,the developed method was successfully used to determine the 10 target analytes in 23 batches of Ganoderma samples from different regions.Results indicated that contents of 10 investigated analytes in each sample showed obvious variation.The principal components analysis(PCA)and hierarchical cluster analysis(HCA)analysis classified the samples into three groups,and the HCA tree visualized the relationships which was mainly contributed by geographical partition.The results indicated geographical origin to be an important factor that affect the accumulation of nucleosides and bases in Ganoderma.In summary,this study provides a simple and practical strategy for quality assessment and cultivation reference of Ganoderma.展开更多
基金supported by the Natural Science Foundation of Sichuan Province(Grant No.:2023NSFSC1783)the National Natural Science Foundation of China(Grant No.:82274372).
文摘Age-related macular degeneration(AMD)represents a predominant cause of blindness among older adults,with limited therapeutic options currently available.Oxidative stress,inflammation,and retinal pigment epithelium injury are recognized as key contributors to the pathogenesis of AMD.Regulated cell death plays a pivotal role in mediating cellular responses to stress,maintaining tissue homeostasis,and contributing to disease progression.Recent research has elucidated several regulated cell death pathwaysdsuch as apoptosis,ferroptosis,pyroptosis,necroptosis,and autophagydthat may contribute to the progression of AMD owing to cell death in the retinal pigment epithelium.These discoveries open new avenues for therapeutic interventions in patients with AMD.In this review,we provide a comprehensive summary and analysis of the latest advancements regarding the relationship between regulated cell death and AMD.Moreover,we examined the therapeutic potential of targeting regulated cell death pathways for the treatment and prevention of AMD,highlighting their roles as promising targets for future therapeutic strategies.
基金financially supported by the Research Committee of the University of Macao(MYRG2018-00239-ICMS and MYRG2014-00089-ICMS-QRCM)the Macao Science and Technology Development Fund(162/2017/A3)Guangzhou International Science and Technology Cooperation Project(File no.201807010044)。
文摘Ganoderma(lingzhi)is a famous herbal medicine and edible supplement in oriental countries for a long history.In this study,a simple micellar electrokinetic chromatography(MEKC)method was established for the analysis of nucleosides and bases,the major bioactive components in Ganoderma for the first time.By optimizing the borate concentration,the sodium dodecyl sulfate(SDS)concentration and the pH value of running buffer,10 nucleosides and bases achieved an ideal separation.In real sample analysis,the developed method was successfully used to determine the 10 target analytes in 23 batches of Ganoderma samples from different regions.Results indicated that contents of 10 investigated analytes in each sample showed obvious variation.The principal components analysis(PCA)and hierarchical cluster analysis(HCA)analysis classified the samples into three groups,and the HCA tree visualized the relationships which was mainly contributed by geographical partition.The results indicated geographical origin to be an important factor that affect the accumulation of nucleosides and bases in Ganoderma.In summary,this study provides a simple and practical strategy for quality assessment and cultivation reference of Ganoderma.
