Fluorescence lifetime imaging microscopy(FLIM)has been widely used in cell biology to detect biomolecules and their interactions.However,breaking the diffraction limit remains a challenge in FLIM due to the typically ...Fluorescence lifetime imaging microscopy(FLIM)has been widely used in cell biology to detect biomolecules and their interactions.However,breaking the diffraction limit remains a challenge in FLIM due to the typically required photon counting method and the limited photon output of conventional dyes.Here,we introduce semiconducting polymer dots(Pdots)for fluorescence lifetime imaging in expansion microscopy by virtue of their tunable lifetime and huge photon budget.We developed three fluorescent Pdots with average lifetimes ranging from 0.4 to 5 ns by varying the polymer species and compositions.Despite their large spectral overlap,distinctive distributions of the Pdots can be resolved in the lifetime domain.The high fluorescence brightness and large photon output offered by Pdots enable multiplex lifetime imaging in photon-starved expansion microscopy,by which subcellular structures were resolved with a spatial resolution of∼49 nm.This study reveals the potential of the tunable Pdot probes for lifetime multiplex imaging in expansion microscopy.展开更多
Hydrogen as a therapeutic agent has attracted a great deal of attention because of its superior therapeutic outcome on many diseases,including inflammatory injury,tumors,metabolic disorders,and neurological diseases.P...Hydrogen as a therapeutic agent has attracted a great deal of attention because of its superior therapeutic outcome on many diseases,including inflammatory injury,tumors,metabolic disorders,and neurological diseases.Photocatalytic hydrogen evolution has emerged as a promising strategy for hydrogen production and delivery.This review article presents the recent developments in the design and synthesis of conjugated polymer materials,including linear polymers and crosslinked conjugated materials,for photocatalytic hydrogen evolution.Particularly,we focus this review on the development of conjugated polymers as photocatalysts and the resulting hydrogen therapy in the fields of anti-inflammatory,free radical scavenging,and cancer treatment.Finally,this article discusses the future research and perspective of conjugated polymer materials for hydrogen evolution and the potential clinical applications of hydrogen as a therapeutic agent.展开更多
基金supported by the National Key R&D Program of China(Grant No.2020YFA0909000)the National Natural Science Foundation of China(Grant No.62235007,22204070)+3 种基金the Shenzhen Science and Technology Program(Grant No.KQTD20170810111314625,JCYJ20210324115807021,SGDX20211123114002003)the Shenzhen Bay Laboratory(SZBL2021080601002)the Guangdong-Hong Kong Technology Cooperation Funding Scheme(Grant No.GHP/176/21SZ)the Guangdong Provincial Key Laboratory of Advanced Biomaterials(2022B1212010003).
文摘Fluorescence lifetime imaging microscopy(FLIM)has been widely used in cell biology to detect biomolecules and their interactions.However,breaking the diffraction limit remains a challenge in FLIM due to the typically required photon counting method and the limited photon output of conventional dyes.Here,we introduce semiconducting polymer dots(Pdots)for fluorescence lifetime imaging in expansion microscopy by virtue of their tunable lifetime and huge photon budget.We developed three fluorescent Pdots with average lifetimes ranging from 0.4 to 5 ns by varying the polymer species and compositions.Despite their large spectral overlap,distinctive distributions of the Pdots can be resolved in the lifetime domain.The high fluorescence brightness and large photon output offered by Pdots enable multiplex lifetime imaging in photon-starved expansion microscopy,by which subcellular structures were resolved with a spatial resolution of∼49 nm.This study reveals the potential of the tunable Pdot probes for lifetime multiplex imaging in expansion microscopy.
基金Guangdong Provincial Key Laboratory of Advanced Biomaterials,Grant/Award Number:2022B1212010003National Natural Science Foundation of China,Grant/Award Numbers:22204070,62235007+2 种基金National Key Research and Development Program of China,Grant/Award Number:2020YFA0909000Shenzhen Science and Technology Innovation Program,Grant/Award Numbers:JCYJ20210324115807021,KQTD20170810111314625,SGDX20211123114002003Shenzhen Bay Laboratory,Grant/Award Number:SZBL2021080601002。
文摘Hydrogen as a therapeutic agent has attracted a great deal of attention because of its superior therapeutic outcome on many diseases,including inflammatory injury,tumors,metabolic disorders,and neurological diseases.Photocatalytic hydrogen evolution has emerged as a promising strategy for hydrogen production and delivery.This review article presents the recent developments in the design and synthesis of conjugated polymer materials,including linear polymers and crosslinked conjugated materials,for photocatalytic hydrogen evolution.Particularly,we focus this review on the development of conjugated polymers as photocatalysts and the resulting hydrogen therapy in the fields of anti-inflammatory,free radical scavenging,and cancer treatment.Finally,this article discusses the future research and perspective of conjugated polymer materials for hydrogen evolution and the potential clinical applications of hydrogen as a therapeutic agent.
