Peripheral nerve regeneration requires stepwise and well-organized establishment of microenvironment.Since local delivery of VEGF-A in peripheral nerve repair is expected to promote angiogenesis in the microenvironmen...Peripheral nerve regeneration requires stepwise and well-organized establishment of microenvironment.Since local delivery of VEGF-A in peripheral nerve repair is expected to promote angiogenesis in the microenvironment and Schwann cells(SCs)play critical role in nerve repair,combination of VEGF and Schwann cells may lead to efficient peripheral nerve regeneration.VEGF-A overexpressing Schwann cells were established and loaded into the inner wall of hydroxyethyl cellulose/soy protein isolate/polyaniline sponge(HSPS)conduits.When HSPS is mechanically distorted,it still has high durability of strain strength,thus,can accommodate unexpected strain of nerve tissues in motion.A 10 mm nerve defect rat model was used to test the repair performance of the HSPS-SC(VEGF)conduits,meanwhile the HSPS,HSPS-SC,HSPS-VEGF conduits and autografts were worked as controls.The immunofluorescent co-staining of GFP/VEGF-A,Ki67 and MBP showed that the VEGF-A overexpressing Schwann cells could promote the proliferation,migration and differentiation of Schwann cells as the VEGF-A was secreted from the VEGF-A overexpressing Schwann cells.The nerve repair performance of the multifunctional and flexible conduits was examined though rat behavioristics,electrophysiology,nerve innervation to gastrocnemius muscle(GM),toluidine blue(TB)staining,transmission electron microscopy(TEM)and NF200/S100 double staining in the regenerated nerve.The results displayed that the effects on the repair of peripheral nerves in HSPS-SC(VEGF)group was the best among the conduits groups and closed to autografts.HSPS-SC(VEGF)group exhibited notably increased CD31+endothelial cells and activation of VEGFR2/ERK signaling pathway in the regenerated nerve tissues,which probably contributed to the improved nerve regeneration.Altogether,the comprehensive strategy including VEGF overexpressing Schwann cells-mediated and HSPS conduit-guided peripheral nerve repair provides a new avenue for nerve tissue engineering.展开更多
Chronic pain is a major cause of suffering that often accompanies diseases and therapies,affecting approximately 20%of individuals at some point in their lives.However,current treatment modalities,such as anesthetic a...Chronic pain is a major cause of suffering that often accompanies diseases and therapies,affecting approximately 20%of individuals at some point in their lives.However,current treatment modalities,such as anesthetic and antipyretic analgesics,have limitations in terms of efficacy and side effects.Nanomedical technology offers a promising avenue to overcome these challenges and introduce new therapeutic mechanisms.This article reviews the recent research on nanomedicine analgesics,integrating analyses of neuroplasticity changes in neurons and pathways related to the transition from acute to chronic pain.Furthermore,it explores potential future strategies using nanomaterials,aiming to provide a roadmap for new analgesic development and improved clinical pain management.By leveraging nanotechnology,these approaches hold the potential to revolutionize pain treatment by delivering targeted and effective relief while minimizing side effects.展开更多
基金This work was supported by the National Natural Science Foundation of China(Grant No.:NSFC 81871493,81871503)the Medical Science Advancement Program(Clinical Medicine)of Wuhan University(Grant No.:TFLC2018002,2018003)。
文摘Peripheral nerve regeneration requires stepwise and well-organized establishment of microenvironment.Since local delivery of VEGF-A in peripheral nerve repair is expected to promote angiogenesis in the microenvironment and Schwann cells(SCs)play critical role in nerve repair,combination of VEGF and Schwann cells may lead to efficient peripheral nerve regeneration.VEGF-A overexpressing Schwann cells were established and loaded into the inner wall of hydroxyethyl cellulose/soy protein isolate/polyaniline sponge(HSPS)conduits.When HSPS is mechanically distorted,it still has high durability of strain strength,thus,can accommodate unexpected strain of nerve tissues in motion.A 10 mm nerve defect rat model was used to test the repair performance of the HSPS-SC(VEGF)conduits,meanwhile the HSPS,HSPS-SC,HSPS-VEGF conduits and autografts were worked as controls.The immunofluorescent co-staining of GFP/VEGF-A,Ki67 and MBP showed that the VEGF-A overexpressing Schwann cells could promote the proliferation,migration and differentiation of Schwann cells as the VEGF-A was secreted from the VEGF-A overexpressing Schwann cells.The nerve repair performance of the multifunctional and flexible conduits was examined though rat behavioristics,electrophysiology,nerve innervation to gastrocnemius muscle(GM),toluidine blue(TB)staining,transmission electron microscopy(TEM)and NF200/S100 double staining in the regenerated nerve.The results displayed that the effects on the repair of peripheral nerves in HSPS-SC(VEGF)group was the best among the conduits groups and closed to autografts.HSPS-SC(VEGF)group exhibited notably increased CD31+endothelial cells and activation of VEGFR2/ERK signaling pathway in the regenerated nerve tissues,which probably contributed to the improved nerve regeneration.Altogether,the comprehensive strategy including VEGF overexpressing Schwann cells-mediated and HSPS conduit-guided peripheral nerve repair provides a new avenue for nerve tissue engineering.
基金National Natural Science Youth Fund,Grant/Award Number:82202326Natural Science Foundation of Shanghai Municipality,Grant/Award Number:21ZR1405300+4 种基金National Natural Science Foundation of China Youth Fund,Grant/Award Number:52322213Shanghai Rising-Star Program,Grant/Award Number:21QA1400900National Funds for General Projects,Grant/Award Number:52272269Innovation Program of Shanghai Municipal Education Commission,Grant/Award Number:2023ZKZD01Key Program of National Natural Science Foundation of China,Grant/Award Number:22235004。
文摘Chronic pain is a major cause of suffering that often accompanies diseases and therapies,affecting approximately 20%of individuals at some point in their lives.However,current treatment modalities,such as anesthetic and antipyretic analgesics,have limitations in terms of efficacy and side effects.Nanomedical technology offers a promising avenue to overcome these challenges and introduce new therapeutic mechanisms.This article reviews the recent research on nanomedicine analgesics,integrating analyses of neuroplasticity changes in neurons and pathways related to the transition from acute to chronic pain.Furthermore,it explores potential future strategies using nanomaterials,aiming to provide a roadmap for new analgesic development and improved clinical pain management.By leveraging nanotechnology,these approaches hold the potential to revolutionize pain treatment by delivering targeted and effective relief while minimizing side effects.
