In this study,the structure-activity relationship of finger citron polysaccharides(FCPs)and their phosphorylated derivatives(P-FCPs)was investigated.Three FCPs(FCP20,FCP40 and FCP60)were successively fractionated by e...In this study,the structure-activity relationship of finger citron polysaccharides(FCPs)and their phosphorylated derivatives(P-FCPs)was investigated.Three FCPs(FCP20,FCP40 and FCP60)were successively fractionated by ethanol,and were phosphorylated modification to obtain P-FCPs(P-FCP20,P-FCP40 and P-FCP60).FCPs and P-FCPs are acidic heteropolysaccharides composed of mannose,rhamnose,glucose,galactose,arabinose and galacturonic acid,and exhibited different content of neutral sugar,uronic acid,proteins,polyphenols and sulfates.FTIR and NMR analyses determined the structures of FCPs and P-FCPs,indicating that the phosphorylation modification was successful.P-FCPs possessed the smaller molecular weight distribution and particle size,with significant changes in microstructure than FCPs.Additionally,P-FCPs showed significantly increased antioxidant and α-amylase inhibitory activities compared to FCPs.The inhibitory mechanisms of FCP40 and P-FCP40 on α-amylase was a mixed-type manner and dynamic fluorescence quenching.The results demonstrate that phosphorylation modification can alter the structural features and bioactivities of FCPs,providing an approach for the further development of finger citron in the field of medicinal and functional foods.展开更多
Acidic sugarcane leaf polysaccharide(ASLP)has a variety of biological activities,but its macromolecular properties make it difficult for the human body to completely absorb or metabolize quickly in the body,and the ac...Acidic sugarcane leaf polysaccharide(ASLP)has a variety of biological activities,but its macromolecular properties make it difficult for the human body to completely absorb or metabolize quickly in the body,and the action time is short.To solve this problem,acidic sugarcane leaf polysaccharide liposomes(ASLPL)were pre-pared by reverse evaporation method and characterized for encapsulation efficiency,particle size,potential,morphology,rheology and stability.In vitro release and digestion tests were used to verify the sustained release effect and stability of ASLPL.The results showed that ASLPL was spherical,with an encapsulation rate of 86.19%±0.976%,a particle size of 134.57±2.501 nm,and a zeta potential of24.49±1.002 mV,which was a pseudoplastic fluid that was more stable to be stored in a neutral environment at 4℃.The antioxidant,hypo-lipidemic and antibacterial activities of ASLPL were significantly increased compared with those of ASLP.The in vitro release and digestion results showed that ASLPL had obvious sustained release effect and could slow down the digestion of ASLP in vivo and prolong the duration of action.Therefore,the development and utilization of ASLPL can provide a greater development potential for the further application of ASLP in the food and phar-maceutical fields.展开更多
As a waste of sugarcane,the added value of sugarcane leaves has not been fully utilized,and sugarcane leaf polysaccharide is an important active component of sugarcane leaves.Using new extraction methods to study the ...As a waste of sugarcane,the added value of sugarcane leaves has not been fully utilized,and sugarcane leaf polysaccharide is an important active component of sugarcane leaves.Using new extraction methods to study the structure and biological activity of polysaccharides from sugarcane leaves is of great significance for the development and application of sugarcane leaves.To determine the structural characteristics and biological activities of different acidic sugarcane leaf polysaccharides(ASLPs)extracted by microwave-assisted hot alkali extraction(MALE),three new acidic sugarcane leaf polysaccharides(ASLP-50,ASLP-70 and ASLP-90)were precipitated by ethanol.The structure of ASLPs extracted by MALE contains protein,sulfate and uronic acid.ASLP-50(195.88 kDa),ASLP-70(94.62 kDa)and ASLP-90(16.37 kDa)were acidic polysaccharides composed of mannose,galacturonic acid,glucose,arabinose and fucose.The results of XRD and NMR showed that ASLPs were amorphous polysaccharides composed of α-L-Araf-(1→main chain,and its branching points were composed of Arap and GalpA residues.In addition,ASLPs showed significant antioxidant,hypoglycemic,hypolipidemic and antibacterial activities in vitro.The research and application of ASLPs provide a reference for the recycling of sugarcane leaves,which is conducive to the development of food,agriculture and pharmaceutical industries.展开更多
基金financial support from the Guangdong Provincial Medical Research Foundation(A2024095)Scientific Research Project of Guangdong Provincial Bureau of Traditional Chinese Medicine(20231216).
文摘In this study,the structure-activity relationship of finger citron polysaccharides(FCPs)and their phosphorylated derivatives(P-FCPs)was investigated.Three FCPs(FCP20,FCP40 and FCP60)were successively fractionated by ethanol,and were phosphorylated modification to obtain P-FCPs(P-FCP20,P-FCP40 and P-FCP60).FCPs and P-FCPs are acidic heteropolysaccharides composed of mannose,rhamnose,glucose,galactose,arabinose and galacturonic acid,and exhibited different content of neutral sugar,uronic acid,proteins,polyphenols and sulfates.FTIR and NMR analyses determined the structures of FCPs and P-FCPs,indicating that the phosphorylation modification was successful.P-FCPs possessed the smaller molecular weight distribution and particle size,with significant changes in microstructure than FCPs.Additionally,P-FCPs showed significantly increased antioxidant and α-amylase inhibitory activities compared to FCPs.The inhibitory mechanisms of FCP40 and P-FCP40 on α-amylase was a mixed-type manner and dynamic fluorescence quenching.The results demonstrate that phosphorylation modification can alter the structural features and bioactivities of FCPs,providing an approach for the further development of finger citron in the field of medicinal and functional foods.
基金support from the Project of Guangdong Provincial Department of Education-special projects in key areas(2021ZDZX4013)Scientific Research Project of Guangdong Provincial Bureau of Traditional Chinese Medicine(20231216).
文摘Acidic sugarcane leaf polysaccharide(ASLP)has a variety of biological activities,but its macromolecular properties make it difficult for the human body to completely absorb or metabolize quickly in the body,and the action time is short.To solve this problem,acidic sugarcane leaf polysaccharide liposomes(ASLPL)were pre-pared by reverse evaporation method and characterized for encapsulation efficiency,particle size,potential,morphology,rheology and stability.In vitro release and digestion tests were used to verify the sustained release effect and stability of ASLPL.The results showed that ASLPL was spherical,with an encapsulation rate of 86.19%±0.976%,a particle size of 134.57±2.501 nm,and a zeta potential of24.49±1.002 mV,which was a pseudoplastic fluid that was more stable to be stored in a neutral environment at 4℃.The antioxidant,hypo-lipidemic and antibacterial activities of ASLPL were significantly increased compared with those of ASLP.The in vitro release and digestion results showed that ASLPL had obvious sustained release effect and could slow down the digestion of ASLP in vivo and prolong the duration of action.Therefore,the development and utilization of ASLPL can provide a greater development potential for the further application of ASLP in the food and phar-maceutical fields.
基金support from the Guangdong Provincial Department of Science and Technology(51309166049)Guangdong Provincial Academy of Traditional Chinese Medicine(51340669).
文摘As a waste of sugarcane,the added value of sugarcane leaves has not been fully utilized,and sugarcane leaf polysaccharide is an important active component of sugarcane leaves.Using new extraction methods to study the structure and biological activity of polysaccharides from sugarcane leaves is of great significance for the development and application of sugarcane leaves.To determine the structural characteristics and biological activities of different acidic sugarcane leaf polysaccharides(ASLPs)extracted by microwave-assisted hot alkali extraction(MALE),three new acidic sugarcane leaf polysaccharides(ASLP-50,ASLP-70 and ASLP-90)were precipitated by ethanol.The structure of ASLPs extracted by MALE contains protein,sulfate and uronic acid.ASLP-50(195.88 kDa),ASLP-70(94.62 kDa)and ASLP-90(16.37 kDa)were acidic polysaccharides composed of mannose,galacturonic acid,glucose,arabinose and fucose.The results of XRD and NMR showed that ASLPs were amorphous polysaccharides composed of α-L-Araf-(1→main chain,and its branching points were composed of Arap and GalpA residues.In addition,ASLPs showed significant antioxidant,hypoglycemic,hypolipidemic and antibacterial activities in vitro.The research and application of ASLPs provide a reference for the recycling of sugarcane leaves,which is conducive to the development of food,agriculture and pharmaceutical industries.
