Plants can cope with stresses via the"cry-for-help"strategy,but how aboveground insect herbivores induce alterations in the rhizosphere microbiota through eliciting this plant-driven response remains unexplo...Plants can cope with stresses via the"cry-for-help"strategy,but how aboveground insect herbivores induce alterations in the rhizosphere microbiota through eliciting this plant-driven response remains unexplored.In this study,we exposed cabbage plants to aboveground insect herbivory for five sequential planting rounds in the same soil.New cabbage plants,growing in the soils conditioned for five rounds,showed a significant increase in resistance to aboveground insect herbivory.Analyses of microbial communities in the rhizosphere of cabbage plants revealed that this effect was attributed to the accumulation of Pseudomonas in herbivore-conditioned soils.Rhizophere metabolic profiling further identified that some amino acids were present at higher concentrations in the rhizosphere of cabbage plants suffering from insect herbivory.Beneficial Pseudomonas species could be enriched by applying these amino acids.Notably,cabbage plants exhibited the highest resistance to insect herbivory following the application of a synbiotic,a combination of amino acids(prebiotics)and Pseudomonas spp.(probiotics).Moreover,we showed that Pseudomonas activates the jasmonate signaling pathway in the plant,which occurred in salicylic acid-deficient,but not in jasmonic acid-deficient,Arabidopsis thaliana mutants and led to the induction of glucosinolate-based defenses against insect herbivory.Collectively,this work reveals a belowgroundcry-forhelp response in plants induced by aboveground herbivory,enabling the development of a novel synbiotic for plant health maintenance.展开更多
The intricate degradation dynamics exhibited by biodegradable alloys significantly influence host responses during the implantation process,posing challenges in achieving stable osseointegration.It is thus critical to...The intricate degradation dynamics exhibited by biodegradable alloys significantly influence host responses during the implantation process,posing challenges in achieving stable osseointegration.It is thus critical to tailor the biodegradation profiles of these implants to establish a conductive tissue microenvironment for bone tissue regeneration.In this study,we demonstrate that Zn-Li alloy forms a layer of Li-containing degradation products at the bone-implant interface to accommodate the bone regeneration process.During the early inflammatory phase,the controlled release of lithium ions(Li^(+))and zinc ions(Zn^(2+))from the alloy induces chemokine(C-C motif)ligand 5(CCL5)production from macrophages,which promotes the recruitment and differentiation of osteoblastic lineage cells.As a protective bone-implant interface is formed subsequently,the active Zn^(2+)release from Zn-Li alloy is suppressed while Li^(+)continues to exhibit anti-inflammatory effects and inhibit osteoclasto-genesis.Therefore,the presence of Li in Zn-based alloy prevents the prolonged inflammation and fibrous cap-sulation typically seen in pure Zn implants.Our findings offer valuable insights into the development of novel biodegradable implants aimed at achieving osseointegration through bioadaption.展开更多
基金supported by the Natural Science Foundation of China(42322708)the China Agricultural Research System(CARS-23)+2 种基金the Key Research and Development Program of Shanxi Province(202302140601007)the Fundamental Research Funds for the Central Universities(KJJQ2025017)the China Postdoctoral Science Foundation(BX20230160)。
文摘Plants can cope with stresses via the"cry-for-help"strategy,but how aboveground insect herbivores induce alterations in the rhizosphere microbiota through eliciting this plant-driven response remains unexplored.In this study,we exposed cabbage plants to aboveground insect herbivory for five sequential planting rounds in the same soil.New cabbage plants,growing in the soils conditioned for five rounds,showed a significant increase in resistance to aboveground insect herbivory.Analyses of microbial communities in the rhizosphere of cabbage plants revealed that this effect was attributed to the accumulation of Pseudomonas in herbivore-conditioned soils.Rhizophere metabolic profiling further identified that some amino acids were present at higher concentrations in the rhizosphere of cabbage plants suffering from insect herbivory.Beneficial Pseudomonas species could be enriched by applying these amino acids.Notably,cabbage plants exhibited the highest resistance to insect herbivory following the application of a synbiotic,a combination of amino acids(prebiotics)and Pseudomonas spp.(probiotics).Moreover,we showed that Pseudomonas activates the jasmonate signaling pathway in the plant,which occurred in salicylic acid-deficient,but not in jasmonic acid-deficient,Arabidopsis thaliana mutants and led to the induction of glucosinolate-based defenses against insect herbivory.Collectively,this work reveals a belowgroundcry-forhelp response in plants induced by aboveground herbivory,enabling the development of a novel synbiotic for plant health maintenance.
基金supported by National Natural Science Foundation of China/Research Grants Council Joint Research Scheme(N_HKU721/23 to W.Q.and NSFC-RGC 5231101024 to Y.Z.)General Research Fund of the Research Grants Council(17207719,1711322,K.W.K.Y.and 17118425,W.Q.)+7 种基金Hong Kong Innovation Technology Fund(ITS/256/22,W.Q.)Health and Medical Research Fund(21200592,22210832,23220925,K.W.K.Y.,09201466,W.Q.)Collaborative Research Fund of the Research Grants Council(C5044-21G,K.W.K.Y.,C7003-22Y,W.Q.)National Key R&D Program of China(2023YFB3810203,K.W.K.Y)National Natural Sci-ence Foundation of China(U22A20121,51931001,Y.Z.,82201124,W.Q.,32301098,D.S.)Beijing Natural Science Foundation Haidian Orig-inal Innovation Joint Fund(L212014,Y.Z.)Shenzhen Science and Technology Innovation Committee Projects(SGDX20220530111405038 to W.Q.,JCYJ20210324120009026,JCYJ20210324120012034 to K.W.W.Y.)Guangdong Basic and Applied Basic Research Foundation(2023A1515011963,W.Q.).
文摘The intricate degradation dynamics exhibited by biodegradable alloys significantly influence host responses during the implantation process,posing challenges in achieving stable osseointegration.It is thus critical to tailor the biodegradation profiles of these implants to establish a conductive tissue microenvironment for bone tissue regeneration.In this study,we demonstrate that Zn-Li alloy forms a layer of Li-containing degradation products at the bone-implant interface to accommodate the bone regeneration process.During the early inflammatory phase,the controlled release of lithium ions(Li^(+))and zinc ions(Zn^(2+))from the alloy induces chemokine(C-C motif)ligand 5(CCL5)production from macrophages,which promotes the recruitment and differentiation of osteoblastic lineage cells.As a protective bone-implant interface is formed subsequently,the active Zn^(2+)release from Zn-Li alloy is suppressed while Li^(+)continues to exhibit anti-inflammatory effects and inhibit osteoclasto-genesis.Therefore,the presence of Li in Zn-based alloy prevents the prolonged inflammation and fibrous cap-sulation typically seen in pure Zn implants.Our findings offer valuable insights into the development of novel biodegradable implants aimed at achieving osseointegration through bioadaption.
