In this paper, the basic reproduction number is calculated for Huanglongbing (HLB) model with impulses which is a vector-borne epidemic model with impulses. For controlling HLB, farmers’ experience is replanting of h...In this paper, the basic reproduction number is calculated for Huanglongbing (HLB) model with impulses which is a vector-borne epidemic model with impulses. For controlling HLB, farmers’ experience is replanting of healthy plants and removing infected plants. To reflect the real world, we construct an impulsive control model which considers replanting of healthy plants and removing infected plants at one fixed time. By analyzing the model, we conclude that the disease-free equilibrium is globally asymptotically stable if the basic reproduction number Ro 1, and we prove that the HLB is permanence if the basic reproduction number Ro > 1.展开更多
Objective： The aim of this study was to establish reproducible two-dimensional electrophoretic assay used for profiling and identification of differentially expressed proteins in human stage I lung adenocarcinoma and...Objective： The aim of this study was to establish reproducible two-dimensional electrophoretic assay used for profiling and identification of differentially expressed proteins in human stage I lung adenocarcinoma and paired normal tumor-adjacent tissue. Methods： The proteins from 12 human stage I lung adenocarcinoma tissues and normal tumor-adjacent tissues were separated using isoelectric focusing electrophoresis （the first dimension） and the subsequent homogeneous SDS-polyacrylamide gel electrophoresis （SDS-PAGE） （the second dimension）. The differentially expressed proteins were determined with PDQuest image analysis software, and identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS） and database searching. Results： The well-reproducible 2-DE gel patterns of human stage I lung adenocarcinoma and normal tumor-adjacent tissues were profiled and 26 differentially expressed proteins uncovered. Nine of these 26 protein spots were cut out from the preparation gels and determined with MALDI-TOF-MS. Searching against the protein database, four candidate proteins were identified. They were 60S acidic ribosomal protein P2, Cathepsin B1, Apolipoprotein A-I precursor, and La 4.1 protein. Conclusion： In this study, high reproducible 2-DE gel protein images of human stage I lung adenocarcinoma and paired normal tumor-adjacent tissues were achieved successfully, and 4 differentially expressed proteins were revealed. These data will be helpful for screen of early biomarker and study of molecular mechanisms of human lung adenocarcinoma.展开更多
Microplastics,as emerging environmental pollutants,are ubiquitously distributed across aquatic,terrestrial,and atmospheric systems,where their heterogeneous spatiotemporal dynamics are governed by multifactorial inter...Microplastics,as emerging environmental pollutants,are ubiquitously distributed across aquatic,terrestrial,and atmospheric systems,where their heterogeneous spatiotemporal dynamics are governed by multifactorial interactions.The key drivers include the intrinsic properties of microplastics(size,shape,and aging state),environmental physical parameters(hydrological factors,soil structure,meteorological factors,lighting and temperature),chemical factors(pH,inorganic iron,and organic matter),and biological activities(microorganisms and root organisms).These interconnected factors collectively dictate MP transport pathways,retention hotspots,and long-term ecological trajectories.Despite the increasing recognition of the environmental prevalence of microplastics,critical knowledge gaps persist regarding the synergistic mechanisms underlying their cross-media migration and fate.This review synthesizes current insights into the complex interplay of factors influencing MP behavior,with a focus on bridging the mechanistic understanding and real-world scenarios.By establishing a unified framework for the environmental interactions of microplastics,this work advances predictive modeling capabilities and informs targeted strategies for pollution mitigation and ecosystem protection,ultimately supporting robust risk assessment protocols.展开更多
文摘In this paper, the basic reproduction number is calculated for Huanglongbing (HLB) model with impulses which is a vector-borne epidemic model with impulses. For controlling HLB, farmers’ experience is replanting of healthy plants and removing infected plants. To reflect the real world, we construct an impulsive control model which considers replanting of healthy plants and removing infected plants at one fixed time. By analyzing the model, we conclude that the disease-free equilibrium is globally asymptotically stable if the basic reproduction number Ro 1, and we prove that the HLB is permanence if the basic reproduction number Ro > 1.
基金Supported by a grant from the National Natural Sciences Foundation of China (No.30571552)
文摘Objective： The aim of this study was to establish reproducible two-dimensional electrophoretic assay used for profiling and identification of differentially expressed proteins in human stage I lung adenocarcinoma and paired normal tumor-adjacent tissue. Methods： The proteins from 12 human stage I lung adenocarcinoma tissues and normal tumor-adjacent tissues were separated using isoelectric focusing electrophoresis （the first dimension） and the subsequent homogeneous SDS-polyacrylamide gel electrophoresis （SDS-PAGE） （the second dimension）. The differentially expressed proteins were determined with PDQuest image analysis software, and identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS） and database searching. Results： The well-reproducible 2-DE gel patterns of human stage I lung adenocarcinoma and normal tumor-adjacent tissues were profiled and 26 differentially expressed proteins uncovered. Nine of these 26 protein spots were cut out from the preparation gels and determined with MALDI-TOF-MS. Searching against the protein database, four candidate proteins were identified. They were 60S acidic ribosomal protein P2, Cathepsin B1, Apolipoprotein A-I precursor, and La 4.1 protein. Conclusion： In this study, high reproducible 2-DE gel protein images of human stage I lung adenocarcinoma and paired normal tumor-adjacent tissues were achieved successfully, and 4 differentially expressed proteins were revealed. These data will be helpful for screen of early biomarker and study of molecular mechanisms of human lung adenocarcinoma.
基金support from the National Natural Science Foundation of China(No.62175237)the Interdiscipline Team Project under auspices of“Light of West”Program in Chinese Academy of Sciences,China(No.xbzgzdsys-202106)+4 种基金the Young Scholar of Regional Development Program in Chinese Academy of Sciences(No.2021)the Natural Science Foundation of Chongqing,China(Nos.CSTB2023NSCQ-MSX0768 and CSTB2024NSCQ-MSX0510)the Special Funding for Postdoctoral Research Projects in Chongqing,China(No.2023CQBSHTB3110)the Chongqing Water Science and Technology Project,China(No.CQSLK-2022006)the Three Gorges Follow-up Project,China(No.CQS23C00400).
文摘Microplastics,as emerging environmental pollutants,are ubiquitously distributed across aquatic,terrestrial,and atmospheric systems,where their heterogeneous spatiotemporal dynamics are governed by multifactorial interactions.The key drivers include the intrinsic properties of microplastics(size,shape,and aging state),environmental physical parameters(hydrological factors,soil structure,meteorological factors,lighting and temperature),chemical factors(pH,inorganic iron,and organic matter),and biological activities(microorganisms and root organisms).These interconnected factors collectively dictate MP transport pathways,retention hotspots,and long-term ecological trajectories.Despite the increasing recognition of the environmental prevalence of microplastics,critical knowledge gaps persist regarding the synergistic mechanisms underlying their cross-media migration and fate.This review synthesizes current insights into the complex interplay of factors influencing MP behavior,with a focus on bridging the mechanistic understanding and real-world scenarios.By establishing a unified framework for the environmental interactions of microplastics,this work advances predictive modeling capabilities and informs targeted strategies for pollution mitigation and ecosystem protection,ultimately supporting robust risk assessment protocols.
