The structural and operational optimization of gas-liquid stirred bioreactors presents both complexity and critical importance for enhancing mass transfer performance. This study proposes a machine learning (ML)-drive...The structural and operational optimization of gas-liquid stirred bioreactors presents both complexity and critical importance for enhancing mass transfer performance. This study proposes a machine learning (ML)-driven approach to identify key features and predict the volumetric mass transfer coefficient (kLa). Four ML models were adopted and compared for kLa prediction in Newtonian and non-Newtonian fluids by evaluative indices, with CatBoost and XGBoost emerging as the optimal models, respectively. Specifically, it is demonstrated that Catboost has higher prediction accuracy (AARD = 18.84%) than empirical equations by effectively incorporating multidimensional features (structural, impeller, and operational), while simultaneously extending applicability to diverse Newtonian fluids. For non-Newtonian fluids, XGBoost outperforms empirical equations by effectively incorporating fluid rheological parameters (consistency coefficient, power-law index), thereby better capturing shear-thinning behavior. Feature importance analysis further identified rotational speed (for Newtonian fluids) and liquid height (for non-Newtonian fluids) as the key features, while 2D partial dependence analysis establishes quantitative optimization ranges. This ML approach provides an efficient predictive tool for gas-liquid stirred bioreactor design and optimization.展开更多
This study investigated the effectiveness of the BOPPPS teaching model in improving English reading skills among junior high school students in Fujian Province,China.Using a quasi-experimental design,102 ninth-grade s...This study investigated the effectiveness of the BOPPPS teaching model in improving English reading skills among junior high school students in Fujian Province,China.Using a quasi-experimental design,102 ninth-grade students from two classes participated:an experimental group(n=51)taught with BOPPPS for 12 weeks(12 sessions)and a control group(*n=51)receiving traditional instruction.The A2 Key for Schools test measured reading ability pre-and post-intervention.Data analysis(independent/paired t-tests,ANCOVA)revealed that students in the BOPPPS group showed significantly greater improvement in English reading ability compared to the control group.The findings indicate the BOPPPS model is well-suited for developing junior high English reading curricula to enhance student proficiency.展开更多
Elderly patients with systemic disseminated <em>Penicillium marneffei </em>are generally more seriously ill with high mortality. In addition to clear diagnosis and timely treatment, careful nursing through...Elderly patients with systemic disseminated <em>Penicillium marneffei </em>are generally more seriously ill with high mortality. In addition to clear diagnosis and timely treatment, careful nursing throughout the hospitalization is particularly important for the patient’s recovery. Psychological nursing and family emotional support, the treatment of adverse drug reactions, systemic infection wound care, the prevention of pressure sores in long-term bedridden patients, antithrombotic nursing, disinfection isolation and the standard prevention of nosocomial cross infection all play decisive roles in the patient’s prognosis. After one month treatment and medical care, the patient was cured and was discharged from hospital. After discharge, the patient follow-up was carried out up to one year. This patient had completely recovered. In this paper, we report a successful nursing experience of an elderly patient with systemic disseminated <em>Penicillium marneffei.</em> With the summary of nursing key points, it could play a reference in the treatment and rehabilitation of similar patients.展开更多
Coronavirus disease-2019 (COVID-19) caused by novel coronavirus 2019 (2019-nCoV) has led to 199,466,211 confirmed cases, including 4,244,541 deaths by 6:44 pm CEST. This epidemic is now on the period of global outbrea...Coronavirus disease-2019 (COVID-19) caused by novel coronavirus 2019 (2019-nCoV) has led to 199,466,211 confirmed cases, including 4,244,541 deaths by 6:44 pm CEST. This epidemic is now on the period of global outbreak, the control of COVID-19 has severely challenged the world. At the beginning of the outbreak, patients infected or suspected were observed and close contacts were isolated. The country delayed the resumption of work and school and all walks of life are seriously affected. All kinds of true and false information and rumours on the internet exist, aggravating people’s anxiety and restlessness. These factors altogether often induce people to feel various negative emotions and psychological problems. In this paper, a patient with COVID-19 was examined through psychological dynamic observation at the beginning of the epidemic. It was found that in the early stage of the epidemic, due to the lack of clear treatment guidelines, the main treatment methods and psychological problems were the main reasons affecting the recovery of patients. Many uncertain factors, including individual and social factors and quarantine, worry about the prognosis, etc, resulting in anxiety, fear, unacceptance, insomnia, irritability and other pessimistic moods. After 16 days of symptomatic treatment, psychological counselling and adjustments in a timely manner, the patient eventually recovered and was discharged. The discussion of this case could serve as a reference for the treatment and rehabilitation of patients with COVID-19 in other countries and regions.展开更多
Numerous circular RNAs(circ RNAs) have been identified as vital regulators in various cancers. The newly reported circular RNA ubiquitin-associated protein 2(circ UBAP2) is a critical player in cell growth and metasta...Numerous circular RNAs(circ RNAs) have been identified as vital regulators in various cancers. The newly reported circular RNA ubiquitin-associated protein 2(circ UBAP2) is a critical player in cell growth and metastasis in various types of cancers, although its role in colorectal cancer(CRC) has yet to be fully elucidated. We find that circ UBAP2 is upregulated in CRC tissues and cell lines to induce autophagy both in vitro and in vivo. The effects of circ UBAP2 on migration, invasion, and proliferation may be partially related to autophagy. Mechanistically, we uncover that circ UBAP2 can directly interact with mi R-582-5 p and subsequently act as a micro RNA sponge to regulate the expression of the mi R-582-5 p target gene forkhead box protein O1(FOXO1) and downstream signaling molecules, which collectively advance the progression and metastasis of CRC. These results suggest that circ UBAP2 acts as an oncogene via a novel circ UBAP2/mi R-582-5 p/FOXO1 axis, providing a potential biomarker and therapeutic target for CRC management.展开更多
Similar to that of other enteroviruses, the replication of enterovirus 71(EV71) occurs on rearranged membranous structures called replication organelles(ROs). Phosphatidylinositol 4-kinase Ⅲ(PI4KB), which is required...Similar to that of other enteroviruses, the replication of enterovirus 71(EV71) occurs on rearranged membranous structures called replication organelles(ROs). Phosphatidylinositol 4-kinase Ⅲ(PI4KB), which is required by enteroviruses for RO formation, yields phosphatidylinositol-4-phosphate(PI4P) on ROs. PI4P then binds and induces conformational changes in the RNA-dependent RNA polymerase(Rd Rp) to modulate Rd Rp activity. Here, we targeted 3D polymerase, the core enzyme of EV71 ROs, and found that the host factor Annexin A2(ANXA2) can interact with 3 D polymerase and promote the replication of EV71. Then, an experiment showed that the annexin domain of ANXA2, which possesses membranebinding capacity, mediates the interaction of ANXA2 with EV71 3 D polymerase. Further research showed that ANXA2 is localized on ROs and interacts with PI4KB. Overexpression of ANXA2 stimulated the formation of PI4P, and the level of PI4P was decreased in ANXA2-knockout cells. Furthermore, ANXA2, PI4KB, and 3D were shown to be localized to the viral RNA replication site, where they form a higher-order protein complex, and the presence of ANXA2 promoted the PI4 KB-3D interaction. Altogether, our data provide new insight into the role of ANXA2 in facilitating formation of the EV71 RNA replication complex.展开更多
Rare-earth calcium oxyborate crystals(RECa_(4)O(BO_(3))_(3),RECOB,RE:rare-earth elements)are a kind of multifunctional crystal materials.In this work,the temperature dependent behaviors of the electroelastic constants...Rare-earth calcium oxyborate crystals(RECa_(4)O(BO_(3))_(3),RECOB,RE:rare-earth elements)are a kind of multifunctional crystal materials.In this work,the temperature dependent behaviors of the electroelastic constants of NdCOB crystal were investigated over the temperature range of-80-200℃,and their temperature coefficients were evaluated.It is found that NdCOB crystal possesses minimal variation of relative dielectric permittivities(<3%).The temperature coefficient of frequency for ZY cut with width shear vibration mode is in the order of 0.07×10^(-4)/℃.The temperature coefficients of the elastic compliances are obtained to be in the range of-33.0×10^(-4)/℃-32.2×10^(-4)/℃.Particularly,the sand swere found to show low temperature coefficients of the elastic compliances,i.e.1.0×10^(-4)/℃and-0.4×10^(-4)/℃,respectively,indicating the existence of zero temperature coefficient of frequency crystal cut.Furthermore,the electromechanical coupling factors and piezoelectric coefficients as a function of temperature were studied.The electromechanical coupling factor kand piezoelectric coefficient dare determined to be~30.8%and~15.2 pC/N at room temperature,respectively.The large piezoelectric response and zero temperature coefficient of frequency indicate the potential usage of NdCOB crystal for piezoelectric frequency devices over a wide temperature range.展开更多
Cu-based electrocatalysts have become the focus in the field of electrochemical CO_(2) reduction reaction(ECO_(2) RR)due to their ability to produce multicarbon products.However,the research on generating single carbo...Cu-based electrocatalysts have become the focus in the field of electrochemical CO_(2) reduction reaction(ECO_(2) RR)due to their ability to produce multicarbon products.However,the research on generating single carbon products with higher economic feasibility via ECO_(2) RR based on Cu-based electrocatalysts is rather rare,and the roles of the surface architecture and oxides of the electrocatalysts have not been explained exactly.In this work,a two-step method including thermal oxidation and electroreduction is proposed to introduce Cuþinto pure Cu foil to form Cu_(2)O/Cu electrocatalyst.By regulating the surface composition and morphology of the electrocatalyst in this way,the activity of ECO 2 RR to C_(1) products has been greatly improved.The Faradaic efficiency of carbon products of the Cu_(2)O/Cu electrode reaches 84%at?0.7 V vs.RHE with good selectivity for HCOOH and CO.The current density of Cu_(2)O/Cu electrode reaches-12.21 mA cm^(2) at-0.8 V vs.RHE,which is much higher than that of the Cu foil electrode(?0.09 mA cm?2).In-situ Raman characterization shows that Cuþin Cu_(2)O/Cu electrode could inhibit hydrogen generation and promote ECO_(2) RR by stabilizing the adsorption of CO_(2).展开更多
Rationale: Webbed scar contractures deformity caused by burns and other factors will lead to joint disorders and affect the mental health of patients, resulting in a severe decline in quality of life. Rapid, effective...Rationale: Webbed scar contractures deformity caused by burns and other factors will lead to joint disorders and affect the mental health of patients, resulting in a severe decline in quality of life. Rapid, effective and less complicated surgical methods can help patients with post-burn rehabilitation. Objective: This article argues that a modified Z-plasty can quickly improve the range of motion caused by webbed scar contractures in joint areas, including surgical methods, postoperative care and prognosis. Methods and Results: The study took place from 2018 to 2022. Thirty-two patients with joint scar contracture deformity, with a mean age of 32.5 years, were included in the study. All patients underwent contracture scar revision and modified Z-plasty repair under anesthesia. All the flaps survived and the joint function was improved. Compared with the traditional Z-plasty, the duration of the operative procedure of the modified Z-plasty was significantly shorter, more surrounding scar tissue was mobilized, and the effectiveness of postoperative scar contracture release was better. Discussions: The modified Z-plasty for scar contracture deformity in joint area is simple, rapid, effective and easy to manage.展开更多
Multiple myeloma(MM)is the second most common hematological malignancy.The introduction of proteasome inhibitors(PIs)and immunomodulatory drugs(IMiDs)greatly improved the outcomes for these patients.Extramedullary dis...Multiple myeloma(MM)is the second most common hematological malignancy.The introduction of proteasome inhibitors(PIs)and immunomodulatory drugs(IMiDs)greatly improved the outcomes for these patients.Extramedullary disease(EMD),an aggressive manifestation of MM,remains a significant treatment challenge.Recently,active clinical development of remarkable novel agents for MM with new mechanisms of action has begun.These agents show promising anti-MM effects and provide the opportunity to extend the survival of patients.They include next-generation PIs and IMiDs;monoclonal antibodies;chimeric antigen receptor T cells;antibody-drug conjugates;bispecific T cell engager antibodies;and small molecular inhibitors.However,few clinical trials have focused on patients with EMD,and the effectiveness of these agents has not been well evaluated and compared in these patients.In this review,we summarize the efficacy of these novel agents in MM patients with EMD.展开更多
Phase separation(PS)plays a fundamental role in organizing aggregates during the viral lifecycle,providing significant opportunities for in viral disease treatment by inhibiting PS.Intrinsically disordered regions(IDR...Phase separation(PS)plays a fundamental role in organizing aggregates during the viral lifecycle,providing significant opportunities for in viral disease treatment by inhibiting PS.Intrinsically disordered regions(IDRs)have been extensively studied and found to be critical for PS.However,the discovery of small molecules that target residues within IDRs remains underexplored,particularly in the field of pesticides.Herein,we report a novel phytovirucide compound 29,which was screened from a series of vanillin derivatives designed with sulfonylpiperazine motifs.The inactivation efficacy of compound 29 against tomato spotted wilt virus(TSWV)was significantly superior to that of the control agents vanisulfane and ribavirin.Mechanistically,compound 29 binds to the TSWV nucleocapsid protein(NP)at residues Lys68(K68),Thr92(T92),and Arg94(R94),with T92 and R94 located in the IDRs of NP.Mutations at these sites impair the ability to form aggregates.Furthermore,a host factor,GTP(Guanosine Triphosphate)-binding nuclear protein Ran-like(Niben101scf08341g01001,NbRANL),which interacts with NP and promotes its aggregation,was identified.Compound 29 also suppresses the expression of NbRANL,resulting in the dual inhibition of ribonucleoprotein complexes(RNPs)formation.This unique mechanism of action provides insights into IDRs-based virucide discovery.展开更多
Antibiotic adjuvants offer a promising strategy for restoring antibiotic sensitivity,expanding antibacterial spectra,and reducing required dosages.Previously,compound 15 was identified as a potential adjuvant for Poly...Antibiotic adjuvants offer a promising strategy for restoring antibiotic sensitivity,expanding antibacterial spectra,and reducing required dosages.Previously,compound 15 was identified as a potential adjuvant for Polymyxin B(PB)against multidrug-resistant(MDR)Pseudomonas aeruginosa DK2;however,its clinical utility was hindered by high cytotoxicity,uncertain in vivo efficacy,and an unclear synergetic mechanism.To address these challenges,we synthesized and evaluated a series of novel benzamide derivatives,with A22 emerging as a particularly promising candidate.A22 demonstrated potent synergistic activity to PB,minimal cytotoxicity,improved water solubility,and broad-spectrum synergism of polymyxins against various clinically isolated MDR Gram-negative strains.In vivo studies using Caenorhabditis elegans and mouse models further confirmed the efficacy of A22.Moreover,A22 effectively suppressed the development of PB resistance in Pseudomonas aeruginosa DK2.Mechanistic investigations revealed that A22 enhances polymyxins activity by inducing reactive oxygen species production,reducing ATP levels,increasing NOX activity,and inhibiting biofilm formation,leading to bacterial death.These findings position A22 as a highly promising candidate for the development of polymyxin adjuvants,offering a robust approach to combating MDR Gram-negative bacterial infections.展开更多
Model-guided design of dielectric elastomer actuators(DEAs)is essential for enabling their application in soft robotics.However,current modeling methods primarily rely on the finite element method(FEM),which suffers f...Model-guided design of dielectric elastomer actuators(DEAs)is essential for enabling their application in soft robotics.However,current modeling methods primarily rely on the finite element method(FEM),which suffers from low computational efficiency.Additionally,the simulation-to-reality(Sim2Real)gap,mainly arising from variations in material properties and manufacturing processes,poses a significant challenge.In this work,we propose a data-driven modeling framework aimed at accurately and rapidly predicting voltage-induced displacements while minimizing the Sim2Real gap.The framework integrates a multi-layer perceptron(MLP)model,which serves as a computationally efficient surrogate for the FEM model,and a cycle-generative adversarial network(CycleGAN)model,which mitigates the Sim2Real gap by leveraging adversarial learning to process both simulation and experimental data.Dimensional analysis is performed to extend the framework's applicability across different DEA scales.The surrogate model delivers global predictions in just 0.8 s,achieving linear coefficients of determination(R^(2))of 0.99106 for release distance prediction and 0.99375 for actuation distance prediction compared to experimental results.Our model can quickly identify the feasible range of biaxial prestretch ratios required for generating the desired deformation,thereby streamlining the design process.Finally,a soft robotic gripper is designed and fabricated,demonstrating versatile object-grasping capabilities.展开更多
Lithium niobate(LiNbO_(3),LN)crystal is a multi-functional material with favorable piezoelectric,nonlinear optical and electro-optic properties.In this study,the electromechanical properties of the radial extensional(...Lithium niobate(LiNbO_(3),LN)crystal is a multi-functional material with favorable piezoelectric,nonlinear optical and electro-optic properties.In this study,the electromechanical properties of the radial extensional(RE)and the thickness extensional(TE)modes of the congruent LN are studied and the temperature dependent behaviors are revealed.The RE mode electromechanical coupling factors(kp)for the Y-and Z-oriented discs are calculated and found to be 3.8%and 24.7%,respectively,which are nearly the same as the experimental results of 3.8%and 25.2%,respectively.The maximum RE and thickness shear(TS)modes electromechanical coupling factors are obtained to be 47.6%and 68.5%for the Yx/25and Yx/167crystal cuts,respectively.The LN crystal possesses good temperature stability of the electromechanical coupling factors(RE and TE modes)from 20℃ to 500℃,where the variations of kp and kt for the Y-oriented discs are<8.0%and<1.8%,respectively.展开更多
The yttrium calcium oxyborate crystal(YCa_(4)O(BO_(3))_(3),YCOB)has been actively studied for hightemperature piezoelectric sensing applications.In this work,the stability of electric properties of YCOB crystal anneal...The yttrium calcium oxyborate crystal(YCa_(4)O(BO_(3))_(3),YCOB)has been actively studied for hightemperature piezoelectric sensing applications.In this work,the stability of electric properties of YCOB crystal annealed in critical conditions(high-temperatures of 900-1100℃ with a low oxygen partial pressure of 4×10^(-6) atm for 24 h)was investigated and the recovery mechanism for the electrical resisitivity,dielectric permittivity and dielectric loss were studied,taking advantage of the X-ray photoelectron spectra and the first principle calculations.The electrical resistivity of the annealed YCOB crystal was slightly decreased when compared to the pristine counterpart,being(2-5)×10^(7) Ω·cm at 850C.The dielectric permittivity and dielectric loss were found to increase after annealing,showing recoverable behaviours after thermal treatment above 650℃ in air.The calculated vacancy formation energy indicates that the oxygen vacancy is the dominant defects in YCOB.The formation of oxygen vacancy weakens the chemical bonding strength between B(Ca or Y)and O atoms,introduces extra donor levels in the band gap,which excites the electrons to conduction band more easily thus enhances the electrical conductivity and dielectric loss.The recovered electrical properties are believed to be associated with the reduced vacancy defects at elevated temperatures in air.展开更多
Artificial intelligence technology, mainly refers to strengthening the artificial way, so as to combinecomputer technology with product design. Firstly, the auxiliary innovation of bathroomproducts based on artificial...Artificial intelligence technology, mainly refers to strengthening the artificial way, so as to combinecomputer technology with product design. Firstly, the auxiliary innovation of bathroomproducts based on artificial intelligence technology is proposed, then the user characteristicsare analysed, and the auxiliary design framework of bathroom products is designed. Finally, theinnovation model is established to realise the auxiliary innovation design of bathroom products.Experimental results show that the proposed method can effectively improve the interactionefficiency and response time, and reduce the false response.展开更多
Depth measurement and three-dimensional(3D)imaging under complex reflection and transmission conditions are challenging and even impossible for traditional structured light techniques,owing to the precondition of poin...Depth measurement and three-dimensional(3D)imaging under complex reflection and transmission conditions are challenging and even impossible for traditional structured light techniques,owing to the precondition of point-to-point triangulation.Despite recent progress in addressing this problem,there is still no efficient and general solution.Herein,a Fourier dual-slice projection with depth-constrained localization is presented to separate and utilize different illumination and reflection components efficiently,which can significantly decrease the number of projection patterns in each sequence from thousands to fifteen.Subsequently,multi-scale parallel single-pixel imaging(MS-PSI)is proposed based on the established and proven position-invariant theorem,which breaks the local regional assumption and enables dynamic 3D reconstruction.Our methodology successfully unveils unseen-before capabilities such as(1)accurate depth measurement under interreflection and subsurface scattering conditions,(2)dynamic measurement of the time-varying high-dynamic-range scene and through thin volumetric scattering media at a rate of 333 frames per second;(3)two-layer 3D imaging of the semitransparent surface and the object hidden behind it.The experimental results confirm that the proposed method paves the way for dynamic 3D reconstruction under complex optical field reflection and transmission conditions,benefiting imaging and sensing applications in advanced manufacturing,autonomous driving,and biomedical imaging.展开更多
This study addresses the complexities of maritime area information collection,particularly in challenging sea environments,by introducing a multi-agent control model for regional information gathering.Focusing on thre...This study addresses the complexities of maritime area information collection,particularly in challenging sea environments,by introducing a multi-agent control model for regional information gathering.Focusing on three key areas—regional coverage,collaborative exploration,and agent obstacle avoidance—we aim to establish a multi-unmanned ship coverage detection system.For regional coverage,a multi-objective optimization model considering effective area coverage and time efficiency is proposed,utilizing a heuristic simulated annealing algorithm for optimal allocation and path planning,achieving a 99.67%effective coverage rate in simulations.Collaborative exploration is tackled through a comprehensive optimization model,solved using an improved greedy strategy,resulting in a 100%static target detection and correct detection index.Agent obstacle avoidance is enhanced by a collision avoidance model and a distributed underlying collision avoidance algorithm,ensuring autonomous obstacle avoidance without communication or scheduling.Simulations confirm zero collaborative failures.This research offers practical solutions for multi-agent exploration and coverage in unknown sea areas,balancing workload and time efficiency while considering ship dynamics constraints.展开更多
基金supported by the National Natural Science Foundation of China(22494713,22178160,22327809 and 22208141)Natural Science Foundation of Jiangsu Province,China(BK20220349).
文摘The structural and operational optimization of gas-liquid stirred bioreactors presents both complexity and critical importance for enhancing mass transfer performance. This study proposes a machine learning (ML)-driven approach to identify key features and predict the volumetric mass transfer coefficient (kLa). Four ML models were adopted and compared for kLa prediction in Newtonian and non-Newtonian fluids by evaluative indices, with CatBoost and XGBoost emerging as the optimal models, respectively. Specifically, it is demonstrated that Catboost has higher prediction accuracy (AARD = 18.84%) than empirical equations by effectively incorporating multidimensional features (structural, impeller, and operational), while simultaneously extending applicability to diverse Newtonian fluids. For non-Newtonian fluids, XGBoost outperforms empirical equations by effectively incorporating fluid rheological parameters (consistency coefficient, power-law index), thereby better capturing shear-thinning behavior. Feature importance analysis further identified rotational speed (for Newtonian fluids) and liquid height (for non-Newtonian fluids) as the key features, while 2D partial dependence analysis establishes quantitative optimization ranges. This ML approach provides an efficient predictive tool for gas-liquid stirred bioreactor design and optimization.
文摘This study investigated the effectiveness of the BOPPPS teaching model in improving English reading skills among junior high school students in Fujian Province,China.Using a quasi-experimental design,102 ninth-grade students from two classes participated:an experimental group(n=51)taught with BOPPPS for 12 weeks(12 sessions)and a control group(*n=51)receiving traditional instruction.The A2 Key for Schools test measured reading ability pre-and post-intervention.Data analysis(independent/paired t-tests,ANCOVA)revealed that students in the BOPPPS group showed significantly greater improvement in English reading ability compared to the control group.The findings indicate the BOPPPS model is well-suited for developing junior high English reading curricula to enhance student proficiency.
文摘Elderly patients with systemic disseminated <em>Penicillium marneffei </em>are generally more seriously ill with high mortality. In addition to clear diagnosis and timely treatment, careful nursing throughout the hospitalization is particularly important for the patient’s recovery. Psychological nursing and family emotional support, the treatment of adverse drug reactions, systemic infection wound care, the prevention of pressure sores in long-term bedridden patients, antithrombotic nursing, disinfection isolation and the standard prevention of nosocomial cross infection all play decisive roles in the patient’s prognosis. After one month treatment and medical care, the patient was cured and was discharged from hospital. After discharge, the patient follow-up was carried out up to one year. This patient had completely recovered. In this paper, we report a successful nursing experience of an elderly patient with systemic disseminated <em>Penicillium marneffei.</em> With the summary of nursing key points, it could play a reference in the treatment and rehabilitation of similar patients.
文摘Coronavirus disease-2019 (COVID-19) caused by novel coronavirus 2019 (2019-nCoV) has led to 199,466,211 confirmed cases, including 4,244,541 deaths by 6:44 pm CEST. This epidemic is now on the period of global outbreak, the control of COVID-19 has severely challenged the world. At the beginning of the outbreak, patients infected or suspected were observed and close contacts were isolated. The country delayed the resumption of work and school and all walks of life are seriously affected. All kinds of true and false information and rumours on the internet exist, aggravating people’s anxiety and restlessness. These factors altogether often induce people to feel various negative emotions and psychological problems. In this paper, a patient with COVID-19 was examined through psychological dynamic observation at the beginning of the epidemic. It was found that in the early stage of the epidemic, due to the lack of clear treatment guidelines, the main treatment methods and psychological problems were the main reasons affecting the recovery of patients. Many uncertain factors, including individual and social factors and quarantine, worry about the prognosis, etc, resulting in anxiety, fear, unacceptance, insomnia, irritability and other pessimistic moods. After 16 days of symptomatic treatment, psychological counselling and adjustments in a timely manner, the patient eventually recovered and was discharged. The discussion of this case could serve as a reference for the treatment and rehabilitation of patients with COVID-19 in other countries and regions.
基金supported by grants from the National Natural Science Foundation of China(82072819,81972377)Xuzhou Science and Technology Project(KC19024)Jiangsu Research and Practice Innovation of Graduate Students(KYCX19-2213)。
文摘Numerous circular RNAs(circ RNAs) have been identified as vital regulators in various cancers. The newly reported circular RNA ubiquitin-associated protein 2(circ UBAP2) is a critical player in cell growth and metastasis in various types of cancers, although its role in colorectal cancer(CRC) has yet to be fully elucidated. We find that circ UBAP2 is upregulated in CRC tissues and cell lines to induce autophagy both in vitro and in vivo. The effects of circ UBAP2 on migration, invasion, and proliferation may be partially related to autophagy. Mechanistically, we uncover that circ UBAP2 can directly interact with mi R-582-5 p and subsequently act as a micro RNA sponge to regulate the expression of the mi R-582-5 p target gene forkhead box protein O1(FOXO1) and downstream signaling molecules, which collectively advance the progression and metastasis of CRC. These results suggest that circ UBAP2 acts as an oncogene via a novel circ UBAP2/mi R-582-5 p/FOXO1 axis, providing a potential biomarker and therapeutic target for CRC management.
基金This study was supported by the National Science Foundation of China(81971976,81772236)Major Project of Technology Innovation Program of Hubei Province(2018ACA123)。
文摘Similar to that of other enteroviruses, the replication of enterovirus 71(EV71) occurs on rearranged membranous structures called replication organelles(ROs). Phosphatidylinositol 4-kinase Ⅲ(PI4KB), which is required by enteroviruses for RO formation, yields phosphatidylinositol-4-phosphate(PI4P) on ROs. PI4P then binds and induces conformational changes in the RNA-dependent RNA polymerase(Rd Rp) to modulate Rd Rp activity. Here, we targeted 3D polymerase, the core enzyme of EV71 ROs, and found that the host factor Annexin A2(ANXA2) can interact with 3 D polymerase and promote the replication of EV71. Then, an experiment showed that the annexin domain of ANXA2, which possesses membranebinding capacity, mediates the interaction of ANXA2 with EV71 3 D polymerase. Further research showed that ANXA2 is localized on ROs and interacts with PI4KB. Overexpression of ANXA2 stimulated the formation of PI4P, and the level of PI4P was decreased in ANXA2-knockout cells. Furthermore, ANXA2, PI4KB, and 3D were shown to be localized to the viral RNA replication site, where they form a higher-order protein complex, and the presence of ANXA2 promoted the PI4 KB-3D interaction. Altogether, our data provide new insight into the role of ANXA2 in facilitating formation of the EV71 RNA replication complex.
基金Project supported by the National Natural Science Foundation of China(51872165)the Primary Research&Development Plan of Shandong Province(2019JZZY010313)+1 种基金Shandong Provincial Natural Science Foundation(ZR2020KA003)the Shandong Province Innovative Talents Support Program(62350070311104)。
文摘Rare-earth calcium oxyborate crystals(RECa_(4)O(BO_(3))_(3),RECOB,RE:rare-earth elements)are a kind of multifunctional crystal materials.In this work,the temperature dependent behaviors of the electroelastic constants of NdCOB crystal were investigated over the temperature range of-80-200℃,and their temperature coefficients were evaluated.It is found that NdCOB crystal possesses minimal variation of relative dielectric permittivities(<3%).The temperature coefficient of frequency for ZY cut with width shear vibration mode is in the order of 0.07×10^(-4)/℃.The temperature coefficients of the elastic compliances are obtained to be in the range of-33.0×10^(-4)/℃-32.2×10^(-4)/℃.Particularly,the sand swere found to show low temperature coefficients of the elastic compliances,i.e.1.0×10^(-4)/℃and-0.4×10^(-4)/℃,respectively,indicating the existence of zero temperature coefficient of frequency crystal cut.Furthermore,the electromechanical coupling factors and piezoelectric coefficients as a function of temperature were studied.The electromechanical coupling factor kand piezoelectric coefficient dare determined to be~30.8%and~15.2 pC/N at room temperature,respectively.The large piezoelectric response and zero temperature coefficient of frequency indicate the potential usage of NdCOB crystal for piezoelectric frequency devices over a wide temperature range.
基金supported by National Natural Science Foundation of China(No.52071183).
文摘Cu-based electrocatalysts have become the focus in the field of electrochemical CO_(2) reduction reaction(ECO_(2) RR)due to their ability to produce multicarbon products.However,the research on generating single carbon products with higher economic feasibility via ECO_(2) RR based on Cu-based electrocatalysts is rather rare,and the roles of the surface architecture and oxides of the electrocatalysts have not been explained exactly.In this work,a two-step method including thermal oxidation and electroreduction is proposed to introduce Cuþinto pure Cu foil to form Cu_(2)O/Cu electrocatalyst.By regulating the surface composition and morphology of the electrocatalyst in this way,the activity of ECO 2 RR to C_(1) products has been greatly improved.The Faradaic efficiency of carbon products of the Cu_(2)O/Cu electrode reaches 84%at?0.7 V vs.RHE with good selectivity for HCOOH and CO.The current density of Cu_(2)O/Cu electrode reaches-12.21 mA cm^(2) at-0.8 V vs.RHE,which is much higher than that of the Cu foil electrode(?0.09 mA cm?2).In-situ Raman characterization shows that Cuþin Cu_(2)O/Cu electrode could inhibit hydrogen generation and promote ECO_(2) RR by stabilizing the adsorption of CO_(2).
文摘Rationale: Webbed scar contractures deformity caused by burns and other factors will lead to joint disorders and affect the mental health of patients, resulting in a severe decline in quality of life. Rapid, effective and less complicated surgical methods can help patients with post-burn rehabilitation. Objective: This article argues that a modified Z-plasty can quickly improve the range of motion caused by webbed scar contractures in joint areas, including surgical methods, postoperative care and prognosis. Methods and Results: The study took place from 2018 to 2022. Thirty-two patients with joint scar contracture deformity, with a mean age of 32.5 years, were included in the study. All patients underwent contracture scar revision and modified Z-plasty repair under anesthesia. All the flaps survived and the joint function was improved. Compared with the traditional Z-plasty, the duration of the operative procedure of the modified Z-plasty was significantly shorter, more surrounding scar tissue was mobilized, and the effectiveness of postoperative scar contracture release was better. Discussions: The modified Z-plasty for scar contracture deformity in joint area is simple, rapid, effective and easy to manage.
文摘Multiple myeloma(MM)is the second most common hematological malignancy.The introduction of proteasome inhibitors(PIs)and immunomodulatory drugs(IMiDs)greatly improved the outcomes for these patients.Extramedullary disease(EMD),an aggressive manifestation of MM,remains a significant treatment challenge.Recently,active clinical development of remarkable novel agents for MM with new mechanisms of action has begun.These agents show promising anti-MM effects and provide the opportunity to extend the survival of patients.They include next-generation PIs and IMiDs;monoclonal antibodies;chimeric antigen receptor T cells;antibody-drug conjugates;bispecific T cell engager antibodies;and small molecular inhibitors.However,few clinical trials have focused on patients with EMD,and the effectiveness of these agents has not been well evaluated and compared in these patients.In this review,we summarize the efficacy of these novel agents in MM patients with EMD.
基金support from the National Natural Science Foundation of China(Nos.32330087 and 32302388)the Key Technologies R&D Program of Guizhou Province in China(No.2017-5788-1)the Scientific Research Innovation Team of Guizhou University(No.202403).
文摘Phase separation(PS)plays a fundamental role in organizing aggregates during the viral lifecycle,providing significant opportunities for in viral disease treatment by inhibiting PS.Intrinsically disordered regions(IDRs)have been extensively studied and found to be critical for PS.However,the discovery of small molecules that target residues within IDRs remains underexplored,particularly in the field of pesticides.Herein,we report a novel phytovirucide compound 29,which was screened from a series of vanillin derivatives designed with sulfonylpiperazine motifs.The inactivation efficacy of compound 29 against tomato spotted wilt virus(TSWV)was significantly superior to that of the control agents vanisulfane and ribavirin.Mechanistically,compound 29 binds to the TSWV nucleocapsid protein(NP)at residues Lys68(K68),Thr92(T92),and Arg94(R94),with T92 and R94 located in the IDRs of NP.Mutations at these sites impair the ability to form aggregates.Furthermore,a host factor,GTP(Guanosine Triphosphate)-binding nuclear protein Ran-like(Niben101scf08341g01001,NbRANL),which interacts with NP and promotes its aggregation,was identified.Compound 29 also suppresses the expression of NbRANL,resulting in the dual inhibition of ribonucleoprotein complexes(RNPs)formation.This unique mechanism of action provides insights into IDRs-based virucide discovery.
基金supported by the National Natural Science Foundation of China(No.22107023 and 32300033)the Natural Science Foundation of Hainan(No.222QN220,China)the Scientific Research Foundation of Hainan University(No.KYQD(ZR)-22010 and KYQD(ZR)-23006,China).
文摘Antibiotic adjuvants offer a promising strategy for restoring antibiotic sensitivity,expanding antibacterial spectra,and reducing required dosages.Previously,compound 15 was identified as a potential adjuvant for Polymyxin B(PB)against multidrug-resistant(MDR)Pseudomonas aeruginosa DK2;however,its clinical utility was hindered by high cytotoxicity,uncertain in vivo efficacy,and an unclear synergetic mechanism.To address these challenges,we synthesized and evaluated a series of novel benzamide derivatives,with A22 emerging as a particularly promising candidate.A22 demonstrated potent synergistic activity to PB,minimal cytotoxicity,improved water solubility,and broad-spectrum synergism of polymyxins against various clinically isolated MDR Gram-negative strains.In vivo studies using Caenorhabditis elegans and mouse models further confirmed the efficacy of A22.Moreover,A22 effectively suppressed the development of PB resistance in Pseudomonas aeruginosa DK2.Mechanistic investigations revealed that A22 enhances polymyxins activity by inducing reactive oxygen species production,reducing ATP levels,increasing NOX activity,and inhibiting biofilm formation,leading to bacterial death.These findings position A22 as a highly promising candidate for the development of polymyxin adjuvants,offering a robust approach to combating MDR Gram-negative bacterial infections.
基金supported by the National Key Research and Development Program of China(Grant No.2023YFB4706500)the National Natural Science Foundation of China(Grant Nos.52275026,T2293725)+1 种基金the Shanghai Rising-Star Program(Grant No.24QA2704500)the Natural Science Foundation of Shanghai(Grant No.23ZR1427800)。
文摘Model-guided design of dielectric elastomer actuators(DEAs)is essential for enabling their application in soft robotics.However,current modeling methods primarily rely on the finite element method(FEM),which suffers from low computational efficiency.Additionally,the simulation-to-reality(Sim2Real)gap,mainly arising from variations in material properties and manufacturing processes,poses a significant challenge.In this work,we propose a data-driven modeling framework aimed at accurately and rapidly predicting voltage-induced displacements while minimizing the Sim2Real gap.The framework integrates a multi-layer perceptron(MLP)model,which serves as a computationally efficient surrogate for the FEM model,and a cycle-generative adversarial network(CycleGAN)model,which mitigates the Sim2Real gap by leveraging adversarial learning to process both simulation and experimental data.Dimensional analysis is performed to extend the framework's applicability across different DEA scales.The surrogate model delivers global predictions in just 0.8 s,achieving linear coefficients of determination(R^(2))of 0.99106 for release distance prediction and 0.99375 for actuation distance prediction compared to experimental results.Our model can quickly identify the feasible range of biaxial prestretch ratios required for generating the desired deformation,thereby streamlining the design process.Finally,a soft robotic gripper is designed and fabricated,demonstrating versatile object-grasping capabilities.
基金supported by the National Key Research and Development Program of China(2020YFA0710303)the National Natural Science Foundation of China(52002072,U1905215,52072076)the Natural Science Foundation of Fujian Province(2021J01589,2023J05082)。
基金the Primary Research&Development Plan of Shandong Province(2017CXGC0413)the National Natural Science Foundation of China(Grant No.51872165).
文摘Lithium niobate(LiNbO_(3),LN)crystal is a multi-functional material with favorable piezoelectric,nonlinear optical and electro-optic properties.In this study,the electromechanical properties of the radial extensional(RE)and the thickness extensional(TE)modes of the congruent LN are studied and the temperature dependent behaviors are revealed.The RE mode electromechanical coupling factors(kp)for the Y-and Z-oriented discs are calculated and found to be 3.8%and 24.7%,respectively,which are nearly the same as the experimental results of 3.8%and 25.2%,respectively.The maximum RE and thickness shear(TS)modes electromechanical coupling factors are obtained to be 47.6%and 68.5%for the Yx/25and Yx/167crystal cuts,respectively.The LN crystal possesses good temperature stability of the electromechanical coupling factors(RE and TE modes)from 20℃ to 500℃,where the variations of kp and kt for the Y-oriented discs are<8.0%and<1.8%,respectively.
基金financially supported by the Primary Research&Development Plan of Shandong Province(2017CXGC0413)the National Natural Science Foundation of China(51872165).
文摘The yttrium calcium oxyborate crystal(YCa_(4)O(BO_(3))_(3),YCOB)has been actively studied for hightemperature piezoelectric sensing applications.In this work,the stability of electric properties of YCOB crystal annealed in critical conditions(high-temperatures of 900-1100℃ with a low oxygen partial pressure of 4×10^(-6) atm for 24 h)was investigated and the recovery mechanism for the electrical resisitivity,dielectric permittivity and dielectric loss were studied,taking advantage of the X-ray photoelectron spectra and the first principle calculations.The electrical resistivity of the annealed YCOB crystal was slightly decreased when compared to the pristine counterpart,being(2-5)×10^(7) Ω·cm at 850C.The dielectric permittivity and dielectric loss were found to increase after annealing,showing recoverable behaviours after thermal treatment above 650℃ in air.The calculated vacancy formation energy indicates that the oxygen vacancy is the dominant defects in YCOB.The formation of oxygen vacancy weakens the chemical bonding strength between B(Ca or Y)and O atoms,introduces extra donor levels in the band gap,which excites the electrons to conduction band more easily thus enhances the electrical conductivity and dielectric loss.The recovered electrical properties are believed to be associated with the reduced vacancy defects at elevated temperatures in air.
基金the 2022 first phase of the supply and demand docking employment education project of the Ministry of Education College Students Division,project number:20220104052,project name:Research and Practice on Talent Training Model for New Engineering Design Professionals Based on Interdisciplinary and Integration of Industry and EducationThe second batch of industryuniversity cooperative education projects in 2021 of the Higher Education Department of the Ministry of Education,project number:202102321010,project name:Exploration and practice of new engineering product design specialty construction based on multidisciplinary intersection andindustry-education integration.
文摘Artificial intelligence technology, mainly refers to strengthening the artificial way, so as to combinecomputer technology with product design. Firstly, the auxiliary innovation of bathroomproducts based on artificial intelligence technology is proposed, then the user characteristicsare analysed, and the auxiliary design framework of bathroom products is designed. Finally, theinnovation model is established to realise the auxiliary innovation design of bathroom products.Experimental results show that the proposed method can effectively improve the interactionefficiency and response time, and reduce the false response.
基金supported by the National Natural Science Foundation of China(62205226,62075143)the National Postdoctoral Program for Innovative Talents of China(BX2021199)+2 种基金the General Financial Grant from the China Postdoctoral Science Foundation(2022M722290)the Key Science and Technology Research and Development Program of Jiangxi Province(20224AAC01011)the Fundamental Research Funds for Central Universities(2022SCU12010).
文摘Depth measurement and three-dimensional(3D)imaging under complex reflection and transmission conditions are challenging and even impossible for traditional structured light techniques,owing to the precondition of point-to-point triangulation.Despite recent progress in addressing this problem,there is still no efficient and general solution.Herein,a Fourier dual-slice projection with depth-constrained localization is presented to separate and utilize different illumination and reflection components efficiently,which can significantly decrease the number of projection patterns in each sequence from thousands to fifteen.Subsequently,multi-scale parallel single-pixel imaging(MS-PSI)is proposed based on the established and proven position-invariant theorem,which breaks the local regional assumption and enables dynamic 3D reconstruction.Our methodology successfully unveils unseen-before capabilities such as(1)accurate depth measurement under interreflection and subsurface scattering conditions,(2)dynamic measurement of the time-varying high-dynamic-range scene and through thin volumetric scattering media at a rate of 333 frames per second;(3)two-layer 3D imaging of the semitransparent surface and the object hidden behind it.The experimental results confirm that the proposed method paves the way for dynamic 3D reconstruction under complex optical field reflection and transmission conditions,benefiting imaging and sensing applications in advanced manufacturing,autonomous driving,and biomedical imaging.
基金supported in part by the National Natural Science Foundation of China under Grant Nos.72171172 and 92367101the Aeronautical Science Foundation of China under Grant No.2023Z066038001+2 种基金the National Natural Science Foundation of China Basic Science Research Center Program under Grant No.62088101Shanghai Municipal Science and Technology Major Project under Grant No.2021SHZDZX0100Chinese Academy of Engineering,Strategic Research and Consulting Program,under Grant No.2023-XZ-65.
文摘This study addresses the complexities of maritime area information collection,particularly in challenging sea environments,by introducing a multi-agent control model for regional information gathering.Focusing on three key areas—regional coverage,collaborative exploration,and agent obstacle avoidance—we aim to establish a multi-unmanned ship coverage detection system.For regional coverage,a multi-objective optimization model considering effective area coverage and time efficiency is proposed,utilizing a heuristic simulated annealing algorithm for optimal allocation and path planning,achieving a 99.67%effective coverage rate in simulations.Collaborative exploration is tackled through a comprehensive optimization model,solved using an improved greedy strategy,resulting in a 100%static target detection and correct detection index.Agent obstacle avoidance is enhanced by a collision avoidance model and a distributed underlying collision avoidance algorithm,ensuring autonomous obstacle avoidance without communication or scheduling.Simulations confirm zero collaborative failures.This research offers practical solutions for multi-agent exploration and coverage in unknown sea areas,balancing workload and time efficiency while considering ship dynamics constraints.
