OBJECTIVE To investigate the fast antidepressant effect and the underlying mechanism of it.METHODS C57BL/6 mice were exposed to a 6-week chronic unpredictable mild stress(CUMS)protocol,and treated with YY-23 or a posi...OBJECTIVE To investigate the fast antidepressant effect and the underlying mechanism of it.METHODS C57BL/6 mice were exposed to a 6-week chronic unpredictable mild stress(CUMS)protocol,and treated with YY-23 or a positive drug fluoxetine in the last 3weeks,behavioral assessments proceed every week.NMDA-induced current were recorded by whole-cell patch clamp on dissociated hippocampal CA1 neurons of Sprague-Dawley rats.Phosphorylation of the signaling proteins was evaluated by Western blotting.RESULTS The electrophysiological results showed that YY-23 is a non-competitive NMDA receptor antagonist,neither in′use-dependent′nor′voltage-dependent′way,with no prominent effect on AMPA induced current.This antagonism of YY-23 could be partially prevented by proven blockers MK801 and Mg2+,laterally indicating the nature of NMDA receptor antagonist both pharmacologically and physiologically.Behavioral and Biochemical study showed that YY-23 could act as antidepressant and exerted strong inhibitory effect on GSK3β in PFC of CMS depression mice model.CONCLUSION YY-23 showed promising antidepressant effect with rapid onset,which might be related to NMDA receptor antagonism and GSK3β inhibition.展开更多
To investigate the effects of lanthanum chloride on binding of LPS to monocyte and CD14 expression upregulation induced by LPS,human monocytes were analyzed by flow cytometry(FCM).The results indicated that lanthanum ...To investigate the effects of lanthanum chloride on binding of LPS to monocyte and CD14 expression upregulation induced by LPS,human monocytes were analyzed by flow cytometry(FCM).The results indicated that lanthanum chloride could decrease the binding rate of LPS with monocyte significantly.LPS upregulated the expression of CD14 on monocyte in a dose dependant manner,however,lanthanum chloride could inhibit the increase of CD14 expression on monocytes by halves.Cellular & Molecular Immunology. 2004;1(5):392-394.展开更多
基金The project supported by the National Natural Science Foundation(31128009,31171011)the National Science&Technology Major Project″Key New Drug Creation and Manufacturing Program″(2014ZX09102-001-005)the Ministry of Science and Technology(2013CB910601)
文摘OBJECTIVE To investigate the fast antidepressant effect and the underlying mechanism of it.METHODS C57BL/6 mice were exposed to a 6-week chronic unpredictable mild stress(CUMS)protocol,and treated with YY-23 or a positive drug fluoxetine in the last 3weeks,behavioral assessments proceed every week.NMDA-induced current were recorded by whole-cell patch clamp on dissociated hippocampal CA1 neurons of Sprague-Dawley rats.Phosphorylation of the signaling proteins was evaluated by Western blotting.RESULTS The electrophysiological results showed that YY-23 is a non-competitive NMDA receptor antagonist,neither in′use-dependent′nor′voltage-dependent′way,with no prominent effect on AMPA induced current.This antagonism of YY-23 could be partially prevented by proven blockers MK801 and Mg2+,laterally indicating the nature of NMDA receptor antagonist both pharmacologically and physiologically.Behavioral and Biochemical study showed that YY-23 could act as antidepressant and exerted strong inhibitory effect on GSK3β in PFC of CMS depression mice model.CONCLUSION YY-23 showed promising antidepressant effect with rapid onset,which might be related to NMDA receptor antagonism and GSK3β inhibition.
基金This work was supported by National Natural Science Foundation of China(30260107)the Natural Science Foundation of Jiangxi province(024008).
文摘To investigate the effects of lanthanum chloride on binding of LPS to monocyte and CD14 expression upregulation induced by LPS,human monocytes were analyzed by flow cytometry(FCM).The results indicated that lanthanum chloride could decrease the binding rate of LPS with monocyte significantly.LPS upregulated the expression of CD14 on monocyte in a dose dependant manner,however,lanthanum chloride could inhibit the increase of CD14 expression on monocytes by halves.Cellular & Molecular Immunology. 2004;1(5):392-394.
