The intrinsic property of MXenes to adsorb dyes with high Raman scattering cross-sections makes them promising candidates for surface-enhanced Raman scattering(SERS)biosensors.In the study,we report a vanadium carbide...The intrinsic property of MXenes to adsorb dyes with high Raman scattering cross-sections makes them promising candidates for surface-enhanced Raman scattering(SERS)biosensors.In the study,we report a vanadium carbide MXene(V_(2)CT_(x))-based SERS biosensor tag,V_(2)CT_(x)@Thi(thionine)@Au NPs(gold nanoparticles)-Ab(antibody),owing to its large interlayer spacing and superior dye adsorption capacity.The tag V_(2)CT_(x)@Thi@Au NPs-Ab was fully characterized and validated,demonstrating a significantly enhanced Raman signal through both electromagnetic and chemical enhancement mechanisms.Using a handheld Raman spectrometer as a readout tool,the developed handheld SERS biosensor was successfully applied for the detection of viral antigens.The biosensor exhibited excellent linearity(1.562–100 nM)and achieved a low limit of detection(LOD)1.562 nM.Moreover,the biosensor demonstrated good selectivity and stability for detecting the target S protein in saliva samples.Our study highlights the potential of V_(2)CT_(x)MXene as a powerful material for handheld SERS biosensors,paving the way for portable and efficient viral diagnostics.展开更多
基金supported by the National Natural Science Foundation of China(No.82030065 to Qin Zhou)“Spring Goose”Talent Team Support Program of Heilongjiang Province(No.2022CYCX0202)+2 种基金Natural Science Foundation of Heilongjiang Province(Excellent Young Scholars Program,JJ2024YX0560)Funding Program for Preferential Returned Scholars of Heilongjiang Province(No.21032240006)Young Elite Scientist Sponsorship Program of Heilongjiang Province(No.20240NTJ018)
文摘The intrinsic property of MXenes to adsorb dyes with high Raman scattering cross-sections makes them promising candidates for surface-enhanced Raman scattering(SERS)biosensors.In the study,we report a vanadium carbide MXene(V_(2)CT_(x))-based SERS biosensor tag,V_(2)CT_(x)@Thi(thionine)@Au NPs(gold nanoparticles)-Ab(antibody),owing to its large interlayer spacing and superior dye adsorption capacity.The tag V_(2)CT_(x)@Thi@Au NPs-Ab was fully characterized and validated,demonstrating a significantly enhanced Raman signal through both electromagnetic and chemical enhancement mechanisms.Using a handheld Raman spectrometer as a readout tool,the developed handheld SERS biosensor was successfully applied for the detection of viral antigens.The biosensor exhibited excellent linearity(1.562–100 nM)and achieved a low limit of detection(LOD)1.562 nM.Moreover,the biosensor demonstrated good selectivity and stability for detecting the target S protein in saliva samples.Our study highlights the potential of V_(2)CT_(x)MXene as a powerful material for handheld SERS biosensors,paving the way for portable and efficient viral diagnostics.
