Objective:To study the anti-HCV activity and mechanism of Hehuan Yin aqueous extract.Methods:Huh7.5.1 cells were used to establish the HCV2a virus infection model.Cell survival rate(%)and Renilla Luciferase Assay Kit(...Objective:To study the anti-HCV activity and mechanism of Hehuan Yin aqueous extract.Methods:Huh7.5.1 cells were used to establish the HCV2a virus infection model.Cell survival rate(%)and Renilla Luciferase Assay Kit(%)were calculated by Celltiter-GLO Assay for evaluating CC50,EC50 and SI values.To observe the drug resistance of the virus to different concentrations of Hehuan Yin within 72 hours by detecting luciferase activity,western-blot was used to detect the protein expression levels of NS5A,NS3 and NS5B.Results:the CC50,EC50 and SI of Hehuan Yin against HCV2a were 132.50g/ml,1.90g/ml and 67.90 respectively.The EC50 after 24h,48h and 72h administration were 18g/ml,5.8g/ml and 2.3g/ml respectively.Within the range of drug concentration,the aqueous extract Hehuan Yin had inhibitory effect on the expression of NS5A and NS5B proteins in a dose-effect relationship,but had no obvious effect on the expression of NS3 protein.Conclusion:The aqueous extract of Hehuan Yin may inhibit the replication of HCV2a virus by changing the protein expression levels of NS5A and NS5B,and the virus has no tolerance to the aqueous extract of Hehuan Yin.展开更多
In the past few years,the emergence of pre-training models has brought uni-modal fields such as computer vision(CV)and natural language processing(NLP)to a new era.Substantial works have shown that they are beneficial...In the past few years,the emergence of pre-training models has brought uni-modal fields such as computer vision(CV)and natural language processing(NLP)to a new era.Substantial works have shown that they are beneficial for downstream uni-modal tasks and avoid training a new model from scratch.So can such pre-trained models be applied to multi-modal tasks?Researchers have ex-plored this problem and made significant progress.This paper surveys recent advances and new frontiers in vision-language pre-training(VLP),including image-text and video-text pre-training.To give readers a better overall grasp of VLP,we first review its recent ad-vances in five aspects:feature extraction,model architecture,pre-training objectives,pre-training datasets,and downstream tasks.Then,we summarize the specific VLP models in detail.Finally,we discuss the new frontiers in VLP.To the best of our knowledge,this is the first survey focused on VLP.We hope that this survey can shed light on future research in the VLP field.展开更多
Comprehensive Summary(±)-Atrachinenins A—C(1—3),three meroterpenoids possessing novel C_(27) skeletons,were isolated from the rhizomes of Atractylodes chinensis.(±)-Atrachinenin A(1)possesses an unpreceden...Comprehensive Summary(±)-Atrachinenins A—C(1—3),three meroterpenoids possessing novel C_(27) skeletons,were isolated from the rhizomes of Atractylodes chinensis.(±)-Atrachinenin A(1)possesses an unprecedented cage-like skeleton with a fused 6/6/5/5 tetracyclic system(decahy-dro-1,5:4a,7-dimethanobenzo[7]annulene),which may be formed by the intermolecular cyclization of(±)-atrachinenin C(3).展开更多
基金Natural Foundation of Guangdong Province(No.2018A030313308)。
文摘Objective:To study the anti-HCV activity and mechanism of Hehuan Yin aqueous extract.Methods:Huh7.5.1 cells were used to establish the HCV2a virus infection model.Cell survival rate(%)and Renilla Luciferase Assay Kit(%)were calculated by Celltiter-GLO Assay for evaluating CC50,EC50 and SI values.To observe the drug resistance of the virus to different concentrations of Hehuan Yin within 72 hours by detecting luciferase activity,western-blot was used to detect the protein expression levels of NS5A,NS3 and NS5B.Results:the CC50,EC50 and SI of Hehuan Yin against HCV2a were 132.50g/ml,1.90g/ml and 67.90 respectively.The EC50 after 24h,48h and 72h administration were 18g/ml,5.8g/ml and 2.3g/ml respectively.Within the range of drug concentration,the aqueous extract Hehuan Yin had inhibitory effect on the expression of NS5A and NS5B proteins in a dose-effect relationship,but had no obvious effect on the expression of NS3 protein.Conclusion:The aqueous extract of Hehuan Yin may inhibit the replication of HCV2a virus by changing the protein expression levels of NS5A and NS5B,and the virus has no tolerance to the aqueous extract of Hehuan Yin.
基金supported by the Key Research Program of the Chinese Academy of Sciences(No.ZDBSSSW-JSC006)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA 27030300).
文摘In the past few years,the emergence of pre-training models has brought uni-modal fields such as computer vision(CV)and natural language processing(NLP)to a new era.Substantial works have shown that they are beneficial for downstream uni-modal tasks and avoid training a new model from scratch.So can such pre-trained models be applied to multi-modal tasks?Researchers have ex-plored this problem and made significant progress.This paper surveys recent advances and new frontiers in vision-language pre-training(VLP),including image-text and video-text pre-training.To give readers a better overall grasp of VLP,we first review its recent ad-vances in five aspects:feature extraction,model architecture,pre-training objectives,pre-training datasets,and downstream tasks.Then,we summarize the specific VLP models in detail.Finally,we discuss the new frontiers in VLP.To the best of our knowledge,this is the first survey focused on VLP.We hope that this survey can shed light on future research in the VLP field.
基金supported by Macao Science and Technology Development Funds(0008/2019/A,0075/2019/AGJ,and 0023/2019/AKP).
文摘Comprehensive Summary(±)-Atrachinenins A—C(1—3),three meroterpenoids possessing novel C_(27) skeletons,were isolated from the rhizomes of Atractylodes chinensis.(±)-Atrachinenin A(1)possesses an unprecedented cage-like skeleton with a fused 6/6/5/5 tetracyclic system(decahy-dro-1,5:4a,7-dimethanobenzo[7]annulene),which may be formed by the intermolecular cyclization of(±)-atrachinenin C(3).
