The alleviation of chemotherapy-induced myelosuppression is an integral part of sustained and effective cancer therapy.Although the role of the hematopoietic microenvironment in the regulation of hematopoietic stem/pr...The alleviation of chemotherapy-induced myelosuppression is an integral part of sustained and effective cancer therapy.Although the role of the hematopoietic microenvironment in the regulation of hematopoietic stem/progenitor cells(HSPCs)has been widely studied,no drugs that improve hematopoiesis by targeting and modulating the hematopoietic microenvironment have been used clinically.Here,we show that the active small molecule icaritin(ICT)from the Chinese herb Epimedium brevicornum Maxim effectively alleviates chemotherapy-induced hemocytopenia in both mouse and zebrafish models.We demonstrated that ICT enhanced the number and hematopoietic function of HSPCs and that the beneficial effects of ICT occurred indirectly.Single-cell sequencing analysis confirmed that the target cells of ICT in the bone marrow microenvironment were mesenchymal stromal cells(MSCs).In addition,peroxiredoxin 1(PRDX1)was identified as a direct target of ICT.Furthermore,ICT stimulated MSCs to express the effector molecule C-X-C motif chemokine ligand 12(CXCL12)through the PRDX1-reactive oxygen species(ROS)-mitogen-activated protein kinase(MAPK)signaling axis,thereby increasing the number and function of HSPCs.These results suggest that ICT is a promising compound for achieving targeted modulation of the hematopoietic microenvironment to restore hematopoiesis after chemotherapy.展开更多
Since the outbreak of the world-wide novel coronavirus pandemic,crowd counting in public areas,such as in shopping centers and in commercial streets,has gained popularity among public health administrations for preven...Since the outbreak of the world-wide novel coronavirus pandemic,crowd counting in public areas,such as in shopping centers and in commercial streets,has gained popularity among public health administrations for preventing the crowds from gathering.In this paper,we propose a novel adaptive method for crowd counting based on Wi-Fi channel state information(CSI)by using common commercial wireless routers.Compared with previous researches on device-free crowd counting,our proposed method is more adaptive to the change of environ-ment and can achieve high accuracy of crowd count estimation.Because the dis-tance between access point(AP)and monitor point(MP)is typically non-fixed in real-world applications,the strength of received signals varies and makes the tra-ditional amplitude-related models to perform poorly in different environments.In order to achieve adaptivity of the crowd count estimation model,we used convo-lutional neural network(ConvNet)to extract features from correlation coefficient matrix of subcarriers which are insensitive to the change of received signal strength.We conducted experiments in university classroom settings and our model achieved an overall accuracy of 97.79%in estimating a variable number of participants.展开更多
基金funded by the National Natural Science Foundation of China(82141203,82374086,and 82274172)the Shanghai Municipal Science and Technology Major Project(ZD2021CY001)+2 种基金the Three-year Action Plan for Shanghai TCM Development and Inheritance Program(ZY(2021-2023)-0401)the Innovation Team and Talents Cultivation Program of the National Administration of Traditional Chinese Medicine(ZYYCXTDD-202004)the Organizational Key Research and Development Program of Shanghai University of Traditional Chinese Medicine(2023YZZ02).
文摘The alleviation of chemotherapy-induced myelosuppression is an integral part of sustained and effective cancer therapy.Although the role of the hematopoietic microenvironment in the regulation of hematopoietic stem/progenitor cells(HSPCs)has been widely studied,no drugs that improve hematopoiesis by targeting and modulating the hematopoietic microenvironment have been used clinically.Here,we show that the active small molecule icaritin(ICT)from the Chinese herb Epimedium brevicornum Maxim effectively alleviates chemotherapy-induced hemocytopenia in both mouse and zebrafish models.We demonstrated that ICT enhanced the number and hematopoietic function of HSPCs and that the beneficial effects of ICT occurred indirectly.Single-cell sequencing analysis confirmed that the target cells of ICT in the bone marrow microenvironment were mesenchymal stromal cells(MSCs).In addition,peroxiredoxin 1(PRDX1)was identified as a direct target of ICT.Furthermore,ICT stimulated MSCs to express the effector molecule C-X-C motif chemokine ligand 12(CXCL12)through the PRDX1-reactive oxygen species(ROS)-mitogen-activated protein kinase(MAPK)signaling axis,thereby increasing the number and function of HSPCs.These results suggest that ICT is a promising compound for achieving targeted modulation of the hematopoietic microenvironment to restore hematopoiesis after chemotherapy.
基金This work was supported by the National Natural Science Foundation of China(Grant No.61802196,url:http://www.nsfc.gov.cn/)Jiangsu Provincial Government Scholarship for Studying Abroad+1 种基金The Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)NUIST Students’Platform for Innovation and Entrepreneurship Training Program(Grant No.202010300080Y,url:http://sjjx.nuist.edu.cn:81/CXCY/NUIST/).
文摘Since the outbreak of the world-wide novel coronavirus pandemic,crowd counting in public areas,such as in shopping centers and in commercial streets,has gained popularity among public health administrations for preventing the crowds from gathering.In this paper,we propose a novel adaptive method for crowd counting based on Wi-Fi channel state information(CSI)by using common commercial wireless routers.Compared with previous researches on device-free crowd counting,our proposed method is more adaptive to the change of environ-ment and can achieve high accuracy of crowd count estimation.Because the dis-tance between access point(AP)and monitor point(MP)is typically non-fixed in real-world applications,the strength of received signals varies and makes the tra-ditional amplitude-related models to perform poorly in different environments.In order to achieve adaptivity of the crowd count estimation model,we used convo-lutional neural network(ConvNet)to extract features from correlation coefficient matrix of subcarriers which are insensitive to the change of received signal strength.We conducted experiments in university classroom settings and our model achieved an overall accuracy of 97.79%in estimating a variable number of participants.
