Background:Liver injury caused by sepsis seriously impairs the normal physiology of the liver.Wedelactone(WED)has an obvious anti-inflammatory effect against liver damage caused by various factors.Nevertheless,further...Background:Liver injury caused by sepsis seriously impairs the normal physiology of the liver.Wedelactone(WED)has an obvious anti-inflammatory effect against liver damage caused by various factors.Nevertheless,further research is needed to determine if WED might mitigate acute liver damage linked to sepsis by influencing macrophage polarization.Methods:We first assessed the effect of WED on lipopolysaccharides-triggered liver injury by biochemistry assay and tissue staining.Inflammatory factors were assessed using the ELISA kits.The expression of Cluster of Differentiation 86(CD86)and Cluster of Differentiation 206(CD206)was measured by immunofluorescence assay.The protein levels of inducible nitric oxide sythase(iNOS),Arginase 1(Arg-1),phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),PI3K phosphorylation(p-PI3K),AKT phosphorylation(p-AKT),inhibitor of kappa B kinase(IKK),inhibitor of kappa B(IκB),and nuclear factor kappa-B(NF-κB)p65 were quantified by western blot analysis.Results:WED decreased the level of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)and malondialdehyde,and increased the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX).Moreover,WED exerted effective anti-inflammatory effects by decreasing the level of Tumor necrosis factor-α(TNF-α)and Interleukin 6(IL-6)and increasing the level of Interleukin 10(IL-10)in serum and cells.WED not only decreased CD86 and iNOS expression but also increased CD206 and Arg-1 expression.WED also downregulated the increased expression of PI3K,AKT,p-PI3K,p-AKT,IKK,and NF-κB p65 induced by lipopolysaccharides,while up-regulated the decreased expression of IκB.Besides,LY294002 with WED decreased the expression of protein PI3K,AKT,p-PI3K,p-AKT,IKK and NF-κB p65,and raised the expression of IκBα.Conclusion:Wedelolactone could attenuate sepsis-associated acute liver injury,and its mechanism may be associated with balancing pro-inflammatory and anti-inflammatory by the regulation of M1/M2 macrophage polarization via the PI3K/AKT/NF-κB signaling pathway.展开更多
Taking the Qi and Yin deficiency syndrome as an example,the research method of pharmacology of syndrome management system was proposed.By means of text mining,systematic pharmacology and target analysis,to attempt to ...Taking the Qi and Yin deficiency syndrome as an example,the research method of pharmacology of syndrome management system was proposed.By means of text mining,systematic pharmacology and target analysis,to attempt to reveal the essence of the corresponding syndrome by studying the drugs and targets of Qi and Yin deficiency.Fourteen Chinese herbs treating Qi and Yin deficiency were retrieved and used more than 30 times,and 9,317 related targets were predicted.The common targets of action were 85.Topological analysis was carried out by using degree centrality,closeness centrality and betweenness centrality to confirm that estrogen receptor(ESR1),tumor necrosis factor(TNF),D(2)dopamine receptor(DRD2),vitamin D3 receptor(VDR),glucocorticoid receptor(NR3C1),acetylcholinesterase(ACHE)and endothelin-1(EDN1)were highly correlated with Qi and Yin deficiency syndrome.Through the target to find Qi and Yin deficiency syndrome corresponding to 17 categories of diseases.A new idea was provided for studying the biological essence of TCM clinical syndrome differentiation.展开更多
Developing bifunctional nanoplatforms integrating advanced biological imaging with high-performance therapeutic functions is interesting for nanoscience and biomedicine.Herein,thin-layered FePS3 nanosheets modified wi...Developing bifunctional nanoplatforms integrating advanced biological imaging with high-performance therapeutic functions is interesting for nanoscience and biomedicine.Herein,thin-layered FePS3 nanosheets modified with amine polyethylene glycol(FePS3-PEG)are synthesized by ultrasonicated exfoliation of bulk Fe PS3 single crystals under Ar atmosphere and in situ chemical functionalization with amine PEG.The prepared Fe PS3-PEG nanosheets give a photothermal conversion efficiency of 22.1%under 808-nm laser and evident T1relaxation(r1=2.69(mmol L^(-1))^(-1)S^(-1))in external magnetic fields.Biologically experimental results in vitro and in vivo demonstrate that Fe PS3-PEG nanosheets have good biocompatibility and exhibit excellent photothermal therapy(PTT)effect under clear T1-weighted magnetic resonance imaging(MRI).The characteristics of Fe PS3-PEGnanosheets enable them to be a promising nanomedicine for T1-weighted MRI-guided near-infrared PTT of cancers.展开更多
基金supported by the National Natural Science Foundation of China(No.81774190,81903832).
文摘Background:Liver injury caused by sepsis seriously impairs the normal physiology of the liver.Wedelactone(WED)has an obvious anti-inflammatory effect against liver damage caused by various factors.Nevertheless,further research is needed to determine if WED might mitigate acute liver damage linked to sepsis by influencing macrophage polarization.Methods:We first assessed the effect of WED on lipopolysaccharides-triggered liver injury by biochemistry assay and tissue staining.Inflammatory factors were assessed using the ELISA kits.The expression of Cluster of Differentiation 86(CD86)and Cluster of Differentiation 206(CD206)was measured by immunofluorescence assay.The protein levels of inducible nitric oxide sythase(iNOS),Arginase 1(Arg-1),phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),PI3K phosphorylation(p-PI3K),AKT phosphorylation(p-AKT),inhibitor of kappa B kinase(IKK),inhibitor of kappa B(IκB),and nuclear factor kappa-B(NF-κB)p65 were quantified by western blot analysis.Results:WED decreased the level of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)and malondialdehyde,and increased the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX).Moreover,WED exerted effective anti-inflammatory effects by decreasing the level of Tumor necrosis factor-α(TNF-α)and Interleukin 6(IL-6)and increasing the level of Interleukin 10(IL-10)in serum and cells.WED not only decreased CD86 and iNOS expression but also increased CD206 and Arg-1 expression.WED also downregulated the increased expression of PI3K,AKT,p-PI3K,p-AKT,IKK,and NF-κB p65 induced by lipopolysaccharides,while up-regulated the decreased expression of IκB.Besides,LY294002 with WED decreased the expression of protein PI3K,AKT,p-PI3K,p-AKT,IKK and NF-κB p65,and raised the expression of IκBα.Conclusion:Wedelolactone could attenuate sepsis-associated acute liver injury,and its mechanism may be associated with balancing pro-inflammatory and anti-inflammatory by the regulation of M1/M2 macrophage polarization via the PI3K/AKT/NF-κB signaling pathway.
文摘Taking the Qi and Yin deficiency syndrome as an example,the research method of pharmacology of syndrome management system was proposed.By means of text mining,systematic pharmacology and target analysis,to attempt to reveal the essence of the corresponding syndrome by studying the drugs and targets of Qi and Yin deficiency.Fourteen Chinese herbs treating Qi and Yin deficiency were retrieved and used more than 30 times,and 9,317 related targets were predicted.The common targets of action were 85.Topological analysis was carried out by using degree centrality,closeness centrality and betweenness centrality to confirm that estrogen receptor(ESR1),tumor necrosis factor(TNF),D(2)dopamine receptor(DRD2),vitamin D3 receptor(VDR),glucocorticoid receptor(NR3C1),acetylcholinesterase(ACHE)and endothelin-1(EDN1)were highly correlated with Qi and Yin deficiency syndrome.Through the target to find Qi and Yin deficiency syndrome corresponding to 17 categories of diseases.A new idea was provided for studying the biological essence of TCM clinical syndrome differentiation.
基金supported by the National Key Research and Development Program of China(2017YFA0205302)the Key Grant for Special Professors in Jiangsu Province(RK030STP18001)+9 种基金the Key Research and Development Program of Jiangsu(BE2018732)the Natural Science Key Fund for Colleges and Universities in Jiangsu Province(17KJA430011)the National Postdoctoral Program for Innovative Talents(BX20190156)the Scientific Research Foundation of Nanjing University of Posts and Telecommunications(NY218150)the Training Program of the Major Research Plan of the National Natural Science Foundation of China from Nanjing University of Posts and Telecommunications(NY218158)“1311 Talents Program”of Nanjing University of Posts and Telecommunications“100 Top Talents Program”of Sun Yat-sen University(29000-18841216)“Young-Teacher Training Program”of Sun Yat-sen University(29000-31610036)Joint Funds for the Innovation of Science and Technology in Fujian Province(2017Y9111)the Natural Science Foundation of Fujian Province(2020J01613)。
文摘Developing bifunctional nanoplatforms integrating advanced biological imaging with high-performance therapeutic functions is interesting for nanoscience and biomedicine.Herein,thin-layered FePS3 nanosheets modified with amine polyethylene glycol(FePS3-PEG)are synthesized by ultrasonicated exfoliation of bulk Fe PS3 single crystals under Ar atmosphere and in situ chemical functionalization with amine PEG.The prepared Fe PS3-PEG nanosheets give a photothermal conversion efficiency of 22.1%under 808-nm laser and evident T1relaxation(r1=2.69(mmol L^(-1))^(-1)S^(-1))in external magnetic fields.Biologically experimental results in vitro and in vivo demonstrate that Fe PS3-PEG nanosheets have good biocompatibility and exhibit excellent photothermal therapy(PTT)effect under clear T1-weighted magnetic resonance imaging(MRI).The characteristics of Fe PS3-PEGnanosheets enable them to be a promising nanomedicine for T1-weighted MRI-guided near-infrared PTT of cancers.
