Soil acidification is a major threat to agricultural sustainability in tropical and subtropical regions.Biodegradable and environmentally friendly materials,such as calcium lignosulfonate(CaLS),calcium poly(aspartic a...Soil acidification is a major threat to agricultural sustainability in tropical and subtropical regions.Biodegradable and environmentally friendly materials,such as calcium lignosulfonate(CaLS),calcium poly(aspartic acid)(PASP-Ca),and calcium polyγ-glutamic acid(γ-PGA-Ca),are known to effectively ameliorate soil acidity.However,their effectiveness in inhibiting soil acidification has not been studied.This study aimed to evaluate the effect of CaLS,PASP-Ca,andγ-PGA-Ca on the resistance of soil toward acidification as directly and indirectly(i.e.,via nitrification)caused by the application of HNO_(3)and urea,respectively.For comparison,Ca(OH)_(2)and lignin were used as the inorganic and organic controls,respectively.Among the materials,γ-PGA-Ca drove the substantial improvements in the pH buffering capacity(pHBC)of the soil and exhibited the greatest potential in inhibiting HNO_(3)-induced soil acidification via protonation of carboxyl,complexing with Al~(3+),and cation exchange processes.Under acidification induced by urea,CaLS was the optimal one in inhibiting acidification and increasing exchangeable acidity during incubation.Furthermore,the sharp reduction in the population sizes of ammonia-oxidizing bacteria(AOB)and ammonia-oxidizing archaea(AOA)confirmed the inhibition of nitrification via CaLS application.Therefore,compared to improving soil pHBC,CaLS may play a more important role in suppressing indirect acidification.Overall,γ-PGA-Ca was superior to PASP-Ca and CaLS in enhancing the soil pHBC and the its resistance to acidification induced by HNO_(3) addition,whereas CaLS was the best at suppressing urea-driven soil acidification by inhibiting nitrification.In conclusion,these results provide a reference for inhibiting soil re-acidification in intensive agricultural systems.展开更多
Structural health monitoring is important to ensuring the health and safety of dams.An inverse analysis method based on a novel hybrid fireworks algorithm (FWA) and the radial basis function (RBF) model is proposed to...Structural health monitoring is important to ensuring the health and safety of dams.An inverse analysis method based on a novel hybrid fireworks algorithm (FWA) and the radial basis function (RBF) model is proposed to diagnose the health condition of concrete dams.The damage of concrete dams is diagnosed by identifying the elastic modulus of materials using the displacement changes at different reservoir water levels.FWA is a global optimization intelligent algorithm.The proposed hybrid algorithm combines the FWA with the pattern search algorithm, which has a high capability for local optimization.Examples of benchmark functions and pseudo-experiment examples of concrete dams illustrate that the hybrid FWA improves the convergence speed and robustness of the original algorithm.To address the time consumption problem, an RBF-based surrogate model was established to replace part of the finite element method in inverse analysis.Numerical examples of concrete dams illustrate that the use of an RBF-based surrogate model significantly reduces the computation time of inverse analysis with little influence on identification accuracy.The presented hybrid FWA combined with the RBF network can quickly and accurately determine the elastic modulus of materials, and then determine the health status of the concrete dam.展开更多
With widely availed clinically used radionuclides,Cer enkov luminescence imaging(CLI)has become a potential tool in the field of optical molecular imaging.However,the impulse noises introduced by high-energy gamma ray...With widely availed clinically used radionuclides,Cer enkov luminescence imaging(CLI)has become a potential tool in the field of optical molecular imaging.However,the impulse noises introduced by high-energy gamma rays that are generated during the decay of radionuclide reduce the image quality significantly,which affects the acauracy of quantitative analysis,as well as the three dimensional reconstruction.In this work,a novel denoising framework based on fuzzy dlustering and curvat ure driven difusion(CDD)is proposed to remove this kind of impulse noises.To improve the accuracy,the F u1zzy Local Information C-Means algorithm,where spatial information is evolved,is used.We evaluate the per formance of the proposed framework sys-tematically with a series of experiments,and the corresponding results demonstrate a better denoising effect than those from the commonly used median filter method.We hope this work may provide a useful data pre processing tool for CLI and its following studies.展开更多
Brown adipose tissue(BAT)is a kind of adipose tissue engaging in thermoregulatory thermogenesis,metaboloregulatory thermogenesis,and secretory.Current studies have revealed that BAT activity is negatively correlated w...Brown adipose tissue(BAT)is a kind of adipose tissue engaging in thermoregulatory thermogenesis,metaboloregulatory thermogenesis,and secretory.Current studies have revealed that BAT activity is negatively correlated with adult body weight and is considered a target tissue for the treatment of obesity and other metabolic-related diseases.Additionally,the activity of BAT presents certain differences between different ages and genders.Clinically,BAT segmentation based on PET/CT data is a reliable method for brown fat research.However,most of the current BAT segmentation methods rely on the experience of doctors.In this paper,an improved U-net network,ICA-Unet,is proposed to achieve automatic and precise segmentation of BAT.First,the traditional 2D convolution layer in the encoder is replaced with a depth-wise overparameterized convolutional(Do-Conv)layer.Second,the channel attention block is introduced between the double-layer convolution.Finally,the image information entropy(IIE)block is added in the skip connections to strengthen the edge features.Furthermore,the performance of this method is evaluated on the dataset of PET/CT images from 368 patients.The results demonstrate a strong agreement between the automatic segmentation of BAT and manual annotation by experts.The average DICE coeffcient(DSC)is 0.9057,and the average Hausdorff distance is 7.2810.Experimental results suggest that the method proposed in this paper can achieve effcient and accurate automatic BAT segmentation and satisfy the clinical requirements of BAT.展开更多
In recent years,researchers have become focused on the relationship between lipids and bone metabolism balance.Moreover,many diseases related to lipid metabolism dis-orders,such as nonalcoholic fatty liver disease,ath...In recent years,researchers have become focused on the relationship between lipids and bone metabolism balance.Moreover,many diseases related to lipid metabolism dis-orders,such as nonalcoholic fatty liver disease,atherosclerosis,obesity,and menopause,are associated with osteoporotic phenotypes.It has been clinically observed in humans that these lipid metabolism disorders promote changes in osteoporosis-related indicators bone mineral density and bone mass.Furthermore,similar osteoporotic phenotype changes were observed in high-fat and high-cholesterol-induced animal models.Abnormal lipid metabolism(such as increased oxidized lipids and elevated plasma cholesterol)affects bone microenvironment ho-meostasis via cross-organ communication,promoting differentiation of mesenchymal stem cells to adipocytes,and inhibiting commitment towards osteoblasts.Moreover,disturbances in lipid metabolism affect the bone metabolism balance by promoting the secretion of cyto-kines such as receptor activator of nuclear factor-kappa B ligand by osteoblasts and stimulating the differentiation of osteoclasts.Conclusively,this review addresses the possible link be-tween lipid metabolism disorders and osteoporosis and elucidates the potential modulatory mechanisms and signaling pathways by which lipid metabolism affects bone metabolism bal-ance.We also summarize the possible approaches and prospects of intervening lipid meta-bolismforosteoporosistreatment.展开更多
Osteoarthritis(OA)is a progressive degenerative joint sickness related with mechanics,obesity,ageing,etc.,mainly characterized by cartilage degeneration,subchondral bone damage and synovium inflammation.Coordinated me...Osteoarthritis(OA)is a progressive degenerative joint sickness related with mechanics,obesity,ageing,etc.,mainly characterized by cartilage degeneration,subchondral bone damage and synovium inflammation.Coordinated mechanical absorption and conduction of the joint play significant roles in the prevalence and development of OA.Subchondral bone is generally considered a load-burdening tissue where mechanosensitive cells are resident,including osteocytes,osteoblast lineage cells,and osteoclast lineage cells(especially less concerned in mechanical studies).Mechano-signaling imbalances affect complicated cellular events and disorders of subchondral bone homeostasis.This paper will focus on the significance of mechanical force as the pathogenesis,involvement of various mechanical force patterns in mechanosensitive cells,and mechanobiology research of loading devices in vitro and in vivo,which are further discussed.Additionally,various mechanosensing structures(e.g.,transient receptor potential channels,gap junctions,primary cilia,podosome-associated complexes,extracellular vesicles)and mechanotransduction signaling pathways(e.g.,Ca^(2+) signaling,Wnt/β-catenin,RhoA GTPase,focal adhesion kinase,cotranscriptional activators YAP/TAZ)in mechanosensitive bone cells.Finally,we highlight potential targets for improving mechanoprotection in the treatment of OA.These advances furnish an integration of mechanical regulation of subchondral bone homeostasis,as well as OA therapeutic approaches by modulating mechanical homeostasis.展开更多
Osteoporosis is caused by an osteoclast activation mechanism.People suffering from osteoporosis are prone to bone defects.Increasing evidence indicates that scavenging reactive oxygen species(ROS)can inhibit receptor ...Osteoporosis is caused by an osteoclast activation mechanism.People suffering from osteoporosis are prone to bone defects.Increasing evidence indicates that scavenging reactive oxygen species(ROS)can inhibit receptor activator of nuclear factorκB ligand(RANKL)-induced osteoclastogenesis and suppress ovariectomy-induced osteoporosis.It is critical to develop biomaterials with antioxidant properties to modulate osteoclast activity for treating osteoporotic bone defects.Previous studies have shown that manganese(Mn)can improve bone regeneration,and Mn supplementation may treat osteoporosis.However,the effect of Mn on osteoclasts and the role of Mn in osteoporotic bone defects remain unclear.In present research,a model bioceramic,Mn-containedβ-tricalcium phosphate(Mn-TCP)was prepared by introducing Mn intoβ-TCP.The introduction of Mn intoβ-TCP significantly improved the scavenging of oxygen radicals and nitrogen radicals,demonstrating that Mn-TCP bioceramics might have antioxidant properties.The in vitro and in vivo findings revealed that Mn^(2+)ions released from Mn-TCP bioceramics could distinctly inhibit the formation and function of osteoclasts,promote the differentiation of osteoblasts,and accelerate bone regeneration under osteoporotic conditions in vivo.Mechanistically,Mn-TCP bioceramics inhibited osteoclastogenesis and promoted the regeneration of osteoporotic bone defects by scavenging ROS via Nrf2 activation.These results suggest that Mn-containing bioceramics with osteoconductivity,ROS scavenging and bone resorption inhibition abilities may be an ideal biomaterial for the treatment of osteoporotic bone defect.展开更多
Endochondral bone formation is an important route for bone repair.Although emerging evidence has revealed the functions of long non-coding RNAs(lncRNAs)in bone and cartilage development,the effect of lncRNAs in endoch...Endochondral bone formation is an important route for bone repair.Although emerging evidence has revealed the functions of long non-coding RNAs(lncRNAs)in bone and cartilage development,the effect of lncRNAs in endochondral bone repair is still largely unknown.Here,we identified a lncRNA,named Hypertrophic Chondrocyte Angiogenesis-related lncRNA(HCAR),and proved it to promote the endochondral bone repair by upregulating the expression of matrix metallopeptidase 13(Mmp13)and vascular endothelial growth factorα(Vegfa)in hypertrophic chondrocytes.Lnc-HCAR knockdown in hypertrophic chondrocytes restrained the cartilage matrix remodeling and decrease the CD31hiEmcnhi vessels number in a bone repair model.Mechanistically,we proved that lnc-HCAR was mainly enriched in the cytoplasm using fluorescence in situ hybridization(FISH)assay,and it acted as a molecular sponge for miR-15b-5p.Further,in hypertrophic chondrocytes,lnc-HCAR competitively bound to miR-15b-5p to increase Vegfa and Mmp13 expression.Our results proved that lncRNA is deeply involved in endochondral bone repair,which will provide a new theoretical basis for future strategies for promoting fracture healing.展开更多
Spent lithium-ion battery recycling has attracted significant attention because of its importance in regard to the environment and resource importance.Traditional hydrometallurgical methods usually leach all valuable ...Spent lithium-ion battery recycling has attracted significant attention because of its importance in regard to the environment and resource importance.Traditional hydrometallurgical methods usually leach all valuable metals and subsequently extract target meals to prepare corresponding materials.However,Li recovery in these processes requires lengthy operational procedures,and the recovery efficiency is low.In this research,we demonstrate a method to selectively recover lithium before the leaching of other elements by introducing a hydrothermal treatment.Approximately 90%of Li is leached from high-Ni layered oxide cathode powders,while consuming a nearly stoichiometric amount of hydrogen ions.With this selective recovery of Li,the transition metals remain as solid residue hydroxides or oxides.Furthermore,the extraction of Li is found to be highly dependent on the content of transition metals in the cathode materials.A high leaching selectivity of Li(>98%)and nearly 95%leaching efficiency of Li can be reached with LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2).In this case,both the energy and material consumption during the proposed Li recovery is significantly decreased compared to traditional methods;furthermore,the proposed method makes full use of H+to leach Li+.This research is expected to provide new understanding for selectively recovering metal from secondary resources.展开更多
基金supported by the Major project of Ministry of Agriculture and Rural Affairs of the People’s Republic of China(No.NK2022180401)the major project of Ministry of Agriculture and Rural Affairs of the People’s Republic of China(No.NK2022180404)。
文摘Soil acidification is a major threat to agricultural sustainability in tropical and subtropical regions.Biodegradable and environmentally friendly materials,such as calcium lignosulfonate(CaLS),calcium poly(aspartic acid)(PASP-Ca),and calcium polyγ-glutamic acid(γ-PGA-Ca),are known to effectively ameliorate soil acidity.However,their effectiveness in inhibiting soil acidification has not been studied.This study aimed to evaluate the effect of CaLS,PASP-Ca,andγ-PGA-Ca on the resistance of soil toward acidification as directly and indirectly(i.e.,via nitrification)caused by the application of HNO_(3)and urea,respectively.For comparison,Ca(OH)_(2)and lignin were used as the inorganic and organic controls,respectively.Among the materials,γ-PGA-Ca drove the substantial improvements in the pH buffering capacity(pHBC)of the soil and exhibited the greatest potential in inhibiting HNO_(3)-induced soil acidification via protonation of carboxyl,complexing with Al~(3+),and cation exchange processes.Under acidification induced by urea,CaLS was the optimal one in inhibiting acidification and increasing exchangeable acidity during incubation.Furthermore,the sharp reduction in the population sizes of ammonia-oxidizing bacteria(AOB)and ammonia-oxidizing archaea(AOA)confirmed the inhibition of nitrification via CaLS application.Therefore,compared to improving soil pHBC,CaLS may play a more important role in suppressing indirect acidification.Overall,γ-PGA-Ca was superior to PASP-Ca and CaLS in enhancing the soil pHBC and the its resistance to acidification induced by HNO_(3) addition,whereas CaLS was the best at suppressing urea-driven soil acidification by inhibiting nitrification.In conclusion,these results provide a reference for inhibiting soil re-acidification in intensive agricultural systems.
基金supported by the National Key Research and Development Program of China(Grants No.2016YFC0401600 and 2017YFC0404906)the National Natural Science Foundation of China(Grants No.51769033 and 51779035)the Fundamental Research Funds for the Central Universities(Grants No.DUT17ZD205 and DUT19LK14)
文摘Structural health monitoring is important to ensuring the health and safety of dams.An inverse analysis method based on a novel hybrid fireworks algorithm (FWA) and the radial basis function (RBF) model is proposed to diagnose the health condition of concrete dams.The damage of concrete dams is diagnosed by identifying the elastic modulus of materials using the displacement changes at different reservoir water levels.FWA is a global optimization intelligent algorithm.The proposed hybrid algorithm combines the FWA with the pattern search algorithm, which has a high capability for local optimization.Examples of benchmark functions and pseudo-experiment examples of concrete dams illustrate that the hybrid FWA improves the convergence speed and robustness of the original algorithm.To address the time consumption problem, an RBF-based surrogate model was established to replace part of the finite element method in inverse analysis.Numerical examples of concrete dams illustrate that the use of an RBF-based surrogate model significantly reduces the computation time of inverse analysis with little influence on identification accuracy.The presented hybrid FWA combined with the RBF network can quickly and accurately determine the elastic modulus of materials, and then determine the health status of the concrete dam.
基金the Program of the National Natural Science Foundation of China under Grant Nos.61701403,61601363,11571012,61372046 and 61640418the Natural Science Basic Research Plan in Shaanxi Province of China under Grant Nos.2017JQ6006 and 2017JQ6017.
文摘With widely availed clinically used radionuclides,Cer enkov luminescence imaging(CLI)has become a potential tool in the field of optical molecular imaging.However,the impulse noises introduced by high-energy gamma rays that are generated during the decay of radionuclide reduce the image quality significantly,which affects the acauracy of quantitative analysis,as well as the three dimensional reconstruction.In this work,a novel denoising framework based on fuzzy dlustering and curvat ure driven difusion(CDD)is proposed to remove this kind of impulse noises.To improve the accuracy,the F u1zzy Local Information C-Means algorithm,where spatial information is evolved,is used.We evaluate the per formance of the proposed framework sys-tematically with a series of experiments,and the corresponding results demonstrate a better denoising effect than those from the commonly used median filter method.We hope this work may provide a useful data pre processing tool for CLI and its following studies.
基金supported in part by the National Natural Science Foundation of China(61701403,82122033,81871379)National Key Research and Development Program of China(2016YFC0103804,2019YFC1521103,2020YFC1523301,2019YFC-1521102)+3 种基金Key R&D Projects in Shaanxi Province(2019ZDLSF07-02,2019ZDLGY10-01)Key R&D Projects in Qinghai Province(2020-SF-143)China Post-doctoral Science Foundation(2018M643719)Young Talent Support Program of the Shaanxi Association for Science and Technology(20190107).
文摘Brown adipose tissue(BAT)is a kind of adipose tissue engaging in thermoregulatory thermogenesis,metaboloregulatory thermogenesis,and secretory.Current studies have revealed that BAT activity is negatively correlated with adult body weight and is considered a target tissue for the treatment of obesity and other metabolic-related diseases.Additionally,the activity of BAT presents certain differences between different ages and genders.Clinically,BAT segmentation based on PET/CT data is a reliable method for brown fat research.However,most of the current BAT segmentation methods rely on the experience of doctors.In this paper,an improved U-net network,ICA-Unet,is proposed to achieve automatic and precise segmentation of BAT.First,the traditional 2D convolution layer in the encoder is replaced with a depth-wise overparameterized convolutional(Do-Conv)layer.Second,the channel attention block is introduced between the double-layer convolution.Finally,the image information entropy(IIE)block is added in the skip connections to strengthen the edge features.Furthermore,the performance of this method is evaluated on the dataset of PET/CT images from 368 patients.The results demonstrate a strong agreement between the automatic segmentation of BAT and manual annotation by experts.The average DICE coeffcient(DSC)is 0.9057,and the average Hausdorff distance is 7.2810.Experimental results suggest that the method proposed in this paper can achieve effcient and accurate automatic BAT segmentation and satisfy the clinical requirements of BAT.
基金sponsored by the Key Program of the National Natural Science Foundation of China(No.81930067)the Natural Science Foundation of China(No.82002316)the Youth Cultivation Project of Army Medical University(China)(No.2020XQN08).
文摘In recent years,researchers have become focused on the relationship between lipids and bone metabolism balance.Moreover,many diseases related to lipid metabolism dis-orders,such as nonalcoholic fatty liver disease,atherosclerosis,obesity,and menopause,are associated with osteoporotic phenotypes.It has been clinically observed in humans that these lipid metabolism disorders promote changes in osteoporosis-related indicators bone mineral density and bone mass.Furthermore,similar osteoporotic phenotype changes were observed in high-fat and high-cholesterol-induced animal models.Abnormal lipid metabolism(such as increased oxidized lipids and elevated plasma cholesterol)affects bone microenvironment ho-meostasis via cross-organ communication,promoting differentiation of mesenchymal stem cells to adipocytes,and inhibiting commitment towards osteoblasts.Moreover,disturbances in lipid metabolism affect the bone metabolism balance by promoting the secretion of cyto-kines such as receptor activator of nuclear factor-kappa B ligand by osteoblasts and stimulating the differentiation of osteoclasts.Conclusively,this review addresses the possible link be-tween lipid metabolism disorders and osteoporosis and elucidates the potential modulatory mechanisms and signaling pathways by which lipid metabolism affects bone metabolism bal-ance.We also summarize the possible approaches and prospects of intervening lipid meta-bolismforosteoporosistreatment.
基金supported by the Integration Project of NSFC Joint Fund for Regional Innovation and Development(U23A6008)the Key Programme of National Natural Science Foundation of China(81930067)+2 种基金the Youth Program of National Natural Science Foundation of China(82002316)the General Program of Natural Science Foundation of Chongqing(cstc2019jcyj-msxmX0176)the China Postdoctoral Science Foundation(2021MD703946).
文摘Osteoarthritis(OA)is a progressive degenerative joint sickness related with mechanics,obesity,ageing,etc.,mainly characterized by cartilage degeneration,subchondral bone damage and synovium inflammation.Coordinated mechanical absorption and conduction of the joint play significant roles in the prevalence and development of OA.Subchondral bone is generally considered a load-burdening tissue where mechanosensitive cells are resident,including osteocytes,osteoblast lineage cells,and osteoclast lineage cells(especially less concerned in mechanical studies).Mechano-signaling imbalances affect complicated cellular events and disorders of subchondral bone homeostasis.This paper will focus on the significance of mechanical force as the pathogenesis,involvement of various mechanical force patterns in mechanosensitive cells,and mechanobiology research of loading devices in vitro and in vivo,which are further discussed.Additionally,various mechanosensing structures(e.g.,transient receptor potential channels,gap junctions,primary cilia,podosome-associated complexes,extracellular vesicles)and mechanotransduction signaling pathways(e.g.,Ca^(2+) signaling,Wnt/β-catenin,RhoA GTPase,focal adhesion kinase,cotranscriptional activators YAP/TAZ)in mechanosensitive bone cells.Finally,we highlight potential targets for improving mechanoprotection in the treatment of OA.These advances furnish an integration of mechanical regulation of subchondral bone homeostasis,as well as OA therapeutic approaches by modulating mechanical homeostasis.
基金the Key Program of National Natural Science Foundation of China(81930067)the Youth Program of National Natural Science Foundation of China(grant number 82002316)+1 种基金the Youth Cultivation Project of Army Medical University(2020XQN08)General Program of Natural Science Foundation of Chongqing(cstc2019jcyj-msxmX0176).
文摘Osteoporosis is caused by an osteoclast activation mechanism.People suffering from osteoporosis are prone to bone defects.Increasing evidence indicates that scavenging reactive oxygen species(ROS)can inhibit receptor activator of nuclear factorκB ligand(RANKL)-induced osteoclastogenesis and suppress ovariectomy-induced osteoporosis.It is critical to develop biomaterials with antioxidant properties to modulate osteoclast activity for treating osteoporotic bone defects.Previous studies have shown that manganese(Mn)can improve bone regeneration,and Mn supplementation may treat osteoporosis.However,the effect of Mn on osteoclasts and the role of Mn in osteoporotic bone defects remain unclear.In present research,a model bioceramic,Mn-containedβ-tricalcium phosphate(Mn-TCP)was prepared by introducing Mn intoβ-TCP.The introduction of Mn intoβ-TCP significantly improved the scavenging of oxygen radicals and nitrogen radicals,demonstrating that Mn-TCP bioceramics might have antioxidant properties.The in vitro and in vivo findings revealed that Mn^(2+)ions released from Mn-TCP bioceramics could distinctly inhibit the formation and function of osteoclasts,promote the differentiation of osteoblasts,and accelerate bone regeneration under osteoporotic conditions in vivo.Mechanistically,Mn-TCP bioceramics inhibited osteoclastogenesis and promoted the regeneration of osteoporotic bone defects by scavenging ROS via Nrf2 activation.These results suggest that Mn-containing bioceramics with osteoconductivity,ROS scavenging and bone resorption inhibition abilities may be an ideal biomaterial for the treatment of osteoporotic bone defect.
基金This work was supported by Key Program of Natural Science Foundation of China(No.81930067)General Program of Nature Science Foundation of China(No.31870962)+2 种基金the Key Project of Logistics Research Plan of the PLA(No.AWS17J004-02-06)the Medical Science and Technology Youth Cultivation Project of PLA(No.20QNPY022)Medical innovation capability upgrading Plan of Southwest Hospital(No.SWH2018LJ-03).
文摘Endochondral bone formation is an important route for bone repair.Although emerging evidence has revealed the functions of long non-coding RNAs(lncRNAs)in bone and cartilage development,the effect of lncRNAs in endochondral bone repair is still largely unknown.Here,we identified a lncRNA,named Hypertrophic Chondrocyte Angiogenesis-related lncRNA(HCAR),and proved it to promote the endochondral bone repair by upregulating the expression of matrix metallopeptidase 13(Mmp13)and vascular endothelial growth factorα(Vegfa)in hypertrophic chondrocytes.Lnc-HCAR knockdown in hypertrophic chondrocytes restrained the cartilage matrix remodeling and decrease the CD31hiEmcnhi vessels number in a bone repair model.Mechanistically,we proved that lnc-HCAR was mainly enriched in the cytoplasm using fluorescence in situ hybridization(FISH)assay,and it acted as a molecular sponge for miR-15b-5p.Further,in hypertrophic chondrocytes,lnc-HCAR competitively bound to miR-15b-5p to increase Vegfa and Mmp13 expression.Our results proved that lncRNA is deeply involved in endochondral bone repair,which will provide a new theoretical basis for future strategies for promoting fracture healing.
基金the great help on thermodynamics calculation using HSC 6.0 software and the financial support on this research from the National Key Research and Development Program of China(Grant Nos.2017YFB0403300/2017YFB0403305)the National Natural Science Foundation of China under Grant Nos.51874269 and 51934006,and 1000 Talents Program of China(Z.S.)+1 种基金the Innovation Academy for Green Manufacture,Chinese Academy of Sciences(IAGM-2019-A15)the Key Program of Chinese Academy of Sciences(ZDRW_CN_2020-1).
文摘Spent lithium-ion battery recycling has attracted significant attention because of its importance in regard to the environment and resource importance.Traditional hydrometallurgical methods usually leach all valuable metals and subsequently extract target meals to prepare corresponding materials.However,Li recovery in these processes requires lengthy operational procedures,and the recovery efficiency is low.In this research,we demonstrate a method to selectively recover lithium before the leaching of other elements by introducing a hydrothermal treatment.Approximately 90%of Li is leached from high-Ni layered oxide cathode powders,while consuming a nearly stoichiometric amount of hydrogen ions.With this selective recovery of Li,the transition metals remain as solid residue hydroxides or oxides.Furthermore,the extraction of Li is found to be highly dependent on the content of transition metals in the cathode materials.A high leaching selectivity of Li(>98%)and nearly 95%leaching efficiency of Li can be reached with LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2).In this case,both the energy and material consumption during the proposed Li recovery is significantly decreased compared to traditional methods;furthermore,the proposed method makes full use of H+to leach Li+.This research is expected to provide new understanding for selectively recovering metal from secondary resources.
