Cancer is a major threat to human health worldwide.Colorectal cancer(CRC),a highly prevalent malignant tumor,poses a significant public health challenge.Therefore,the identification of effective biomarkers is of great...Cancer is a major threat to human health worldwide.Colorectal cancer(CRC),a highly prevalent malignant tumor,poses a significant public health challenge.Therefore,the identification of effective biomarkers is of great significance[1].The NFKBIE gene encodes an inhibitor of nuclear factorκBε(IkBε).IκBε,a key regulator of the NF-κB signaling pathway,is closely associated with tumorigenesis.However,their roles in CRC remain unclear[2].Pan-cancer research is crucial for accelerating the identification of biomarkers and translational medical research,as it can reveal molecular commonalities and differences among different tumor types[3].展开更多
Mechanism of discontinuous precipitation(DP) in AZ80 alloy was investigated by phase-orientation correlated characterization.The results show DPs nucleate by turning the original grain boundaries(GBs) as reaction fron...Mechanism of discontinuous precipitation(DP) in AZ80 alloy was investigated by phase-orientation correlated characterization.The results show DPs nucleate by turning the original grain boundaries(GBs) as reaction front(RF),and further driving the RF to realize their growth.The DPs regions retained the same orientations as their parent grains.The misorientation angle and rotation axis of RFs had strong influence on DPs nucleation.The low-angle GBs,twin boundaries(TBs) and the GBs with specific misorientation axis which are known as low energy and low mobility GBs can hardly initiate DPs.In addition,the TBs had a strong ability to inhibit the growth of DPs,but it should be noticed that the growth of DPs cannot be totally inhibited by TBs.DPs can engulf the twins when the growth direction is approximately parallel to the long axis of TBs.The inhibition behavior is related to the distribution of Al solute atoms near the RF,boundary interactions of the TBs and twin tips with the RF,and the morphology of the continuous precipitations within the twins.展开更多
A high-purity Ti sheet with dense preexisting twins(introduced by 10%cold rolling)was subjected to isochronal annealing at 500–800°C for 1 h and isothermal annealing at 600°C for 0.17–100 h,respectively.By...A high-purity Ti sheet with dense preexisting twins(introduced by 10%cold rolling)was subjected to isochronal annealing at 500–800°C for 1 h and isothermal annealing at 600°C for 0.17–100 h,respectively.By mainly utilizing electron backscatter diffraction(EBSD)and electron channel contrast(ECC)imaging techniques,the microstructure and texture evolution during the isochronal and isothermal annealing were investigated systematically.Results show that recrystallization nuclei appear in the specimen annealed at 600°C for 1 h.In contrast,recrystallization cannot be initiated for those annealed at lower temperatures or for a shorter time.With the increase in temperature or time,the fraction of the recrystallized structure increases with gradual grain coarsening.Nearly complete recrystallization is reached after 800°C-1 h or 600°C-100 h annealing.Due to the distribution heterogeneity of microstructure and stored energy induced by the dense preexisting twins,recrystallization nucleation preferentially occurs in some specific regions(twin-twin or twin-grain boundary junctions).Then,they selectively consume twin lamellar structures,leading to non-uniform grain growth.It is demonstrated that the recrystallization nucleation is dominated by the strain-induced boundary migration mechanism,allowing scattered texture components corresponding to the twin lamellar structures to be gradually encroached by those untwinned structures with the initial bimodal basal texture(BBT).Eventually,a strong BBT is always obtained after sufficient recrystallization.展开更多
Objective:To analyze the application value of a non-catheter tampon in abdominal hysterectomy,providing a reference for related research.Methods:A total of 100 patients were included in this study,with data collected ...Objective:To analyze the application value of a non-catheter tampon in abdominal hysterectomy,providing a reference for related research.Methods:A total of 100 patients were included in this study,with data collected between January 4,2022,and January 4,2024.The patients were divided into two groups:the new group and the traditional group,each comprising 50 patients.Results:Compared with the traditional group,the new group demonstrated significantly lower intraoperative blood loss(P<0.05).Additionally,the incidence of complications,operation time,hospital stay,time required to resume normal activities,and postoperative VAS scores were all significantly lower in the new group(P<0.05).Conclusion:The application of a non-catheter tampon during abdominal hysterectomy yields satisfactory results.This approach is worthy of further clinical promotion and application.展开更多
Transferring healthy and functional mitochondria to the lateral ventricles confers neuroprotection in a rat model of ischemia-reperfusion injury.Autologous mitochondrial transplantation is also beneficial in pediatric...Transferring healthy and functional mitochondria to the lateral ventricles confers neuroprotection in a rat model of ischemia-reperfusion injury.Autologous mitochondrial transplantation is also beneficial in pediatric patients with cardiac ischemia-reperfusion injury.Thus,transplantation of functional exogenous mitochondria may be a promising therapeutic approach for ischemic disease.To explore the neuroprotective effect of mitochondria transplantation and determine the underlying mechanism in ischemic stroke,in this study we established a photo-thrombosis-induced mouse model of focal ischemia and administered freshly isolated mitochondria via the tail vein or to the injury site(in situ).Animal behavior tests,immunofluorescence staining,2,3,5-triphenyltetrazolium chloride(TTC)staining,mRNA-seq,and western blotting were used to assess mouse anxiety and memory,cortical infarct area,pyroptosis,and neurogenesis,respectively.Using bioinformatics analysis,western blotting,co-immunoprecipitation,and mass spectroscopy,we identified S100 calcium binding protein A9(S100A9)as a potential regulator of mitochondrial function and determined its possible interacting proteins.Interactions between exogenous and endogenous mitochondria,as well as the effect of exogenous mitochondria on recipient microglia,were assessed in vitro.Our data showed that:(1)mitochondrial transplantation markedly reduced mortality and improved emotional and cognitive function,as well as reducing infarct area,inhibiting pyroptosis,and promoting cortical neurogenesis;(2)microglial expression of S100A9 was markedly increased by ischemic injury and regulated mitochondrial function;(3)in vitro,exogenous mitochondria enhanced mitochondrial function,reduced redox stress,and regulated microglial polarization and pyroptosis by fusing with endogenous mitochondria;and(4)S100A9 promoted internalization of exogenous mitochondria by the microglia,thereby amplifying their pro-proliferation and anti-inflammatory effects.Taken together,our findings show that mitochondrial transplantation protects against the deleterious effects of ischemic stroke by suppressing pyroptosis and promoting neurogenesis,and that S100A9 plays a vital role in promoting internalization of exogenous mitochondria.展开更多
In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino acids.The tea plant(Camellia sinensis)is a perennial evergreen wit...In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino acids.The tea plant(Camellia sinensis)is a perennial evergreen with a relatively high level of amino acids.However,systematic identification and molecular characterization of the LHT gene family has rarely been reported in tea plants.In this study,22 CsLHTs were identified from the‘Shuchazao’genome and classified into two groups.The modeled three-dimensional structure and the conserved domains presented a high similarity among the LHTs proteins.Moreover,it was predicted that a few genes were conserved through the analysis of the physiochemical characters,structures and cis-elements in promoters.The expression patterns in tea plants revealed that CsLHT7 was mainly expressed in the roots,and CsLHT4 and CsLHT11 exhibited relatively high expression in both the roots and leaves.Moreover,the expression of all three genes could be induced by organic nitrogen.Additionally,heterogeneous expression of CsLHT4,CsLHT7 and CsLHT11 in Arabidopsis thaliana decreased the aerial parts biomass compared with that in WT plants while significantly increased the rosette biomass only for CsLHT11transgenic plants versus WT plants.Overall,our results provide fundamental information about CsLHTs and potential genes in N utilization for further analysis in tea plants.展开更多
BACKGROUND The mucosal barrier's immune-brain interactions,pivotal for neural development and function,are increasingly recognized for their potential causal and therapeutic relevance to irritable bowel syndrome(I...BACKGROUND The mucosal barrier's immune-brain interactions,pivotal for neural development and function,are increasingly recognized for their potential causal and therapeutic relevance to irritable bowel syndrome(IBS).Prior studies linking immune inflammation with IBS have been inconsistent.To further elucidate this relationship,we conducted a Mendelian randomization(MR)analysis of 731 immune cell markers to dissect the influence of various immune phenotypes on IBS.Our goal was to deepen our understanding of the disrupted brain-gut axis in IBS and to identify novel therapeutic targets.AIM To leverage publicly available data to perform MR analysis on 731 immune cell markers and explore their impact on IBS.We aimed to uncover immunophenotypic associations with IBS that could inform future drug development and therapeutic strategies.METHODS We performed a comprehensive two-sample MR analysis to evaluate the causal relationship between immune cell markers and IBS.By utilizing genetic data from public databases,we examined the causal associations between 731 immune cell markers,encompassing median fluorescence intensity,relative cell abundance,absolute cell count,and morphological parameters,with IBS susceptibility.Sensitivity analyses were conducted to validate our findings and address potential heterogeneity and pleiotropy.RESULTS Bidirectional false discovery rate correction indicated no significant influence of IBS on immunophenotypes.However,our analysis revealed a causal impact of IBS on 30 out of 731 immune phenotypes(P<0.05).Nine immune phenotypes demonstrated a protective effect against IBS[inverse variance weighting(IVW)<0.05,odd ratio(OR)<1],while 21 others were associated with an increased risk of IBS onset(IVW≥0.05,OR≥1).CONCLUSION Our findings underscore a substantial genetic correlation between immune cell phenotypes and IBS,providing valuable insights into the pathophysiology of the condition.These results pave the way for the development of more precise biomarkers and targeted therapies for IBS.Furthermore,this research enriches our comprehension of immune cell roles in IBS pathogenesis,offering a foundation for more effective,personalized treatment approaches.These advancements hold promise for improving IBS patient quality of life and reducing the disease burden on individuals and their families.展开更多
LZ91 Mg-Li alloy plates with three types of initial texture were rolled by 70%reduction at both room temperature and 200℃to explore the rolling texture formation ofα-Mg phase.The results showed that the rolling text...LZ91 Mg-Li alloy plates with three types of initial texture were rolled by 70%reduction at both room temperature and 200℃to explore the rolling texture formation ofα-Mg phase.The results showed that the rolling texture is largely affected by the initial texture.All the samples exhibited two main texture components as RD-split double peaks texture and TD-split double peaks texture after large strain rolling.The intensity of the two texture components was strongly influenced by the initial orientation and rolling temperature.Extension twinning altered the large-split non-basal orientation to a near basal one at low rolling strain.The basal orientation induced by twinning is unstable,which finally transmitted to the RD-split texture.The strong TD-split texture formed due to slip-induced orientation transition from its initial orientation.The competition between prismatic and basal slip determined the intensity and tilt angle of the TD-split texture.By increasing the rolling temperature,the TD-split texture component was enhanced in all three samples.Limitation of extension twinning behavior and the promotion of prismatic slip at elevated temperature are the main reasons for the difference in hot and cold rolling texture.展开更多
Sodium-ion batteries(SIBs) and hybrid capacitors(SIHCs) have garnered significant attention in energy storage due to their inherent advantages,including high energy density,cost-effectiveness,and enhanced safety.Howev...Sodium-ion batteries(SIBs) and hybrid capacitors(SIHCs) have garnered significant attention in energy storage due to their inherent advantages,including high energy density,cost-effectiveness,and enhanced safety.However,developing high-performance anode materials to improve sodium storage performa nce still remains a major challenge.Here,a facile one-pot method has been developed to fabricate a hybrid of MoSeTe nanosheets implanted within the N,F co-doped honeycomb carbon skeleton(MoSeTe/N,F@C).Experimental results demonstrate that the incorporation of large-sized Te atoms into MoSeTe nanosheets enlarges the layer spacing and creates abundant anion vacancies,which effectively facilitate the insertion/extraction of Na^(+) and provide numerous ion adsorption sites for rapid surface capacitive behavior.Additionally,the heteroatoms N,F co-doped honeycomb carbon skeleton with a highly conductive network can restrain the volume expansion and boost reaction kinetics within the electrode.As anticipated,the MoSeTe/N,F@C anode exhibits high reversible capacities along with exceptional cycle stability.When coupled with Na_(3)V_(2)(PO_(4))_(3)@C(NVPF@C) to form SIB full cells,the anode delivers a reversible specific capacity of 126 mA h g^(-1) after 100 cycles at 0.1 A g^(-1).Furthermore,when combined with AC to form SIHC full cells,the anode demonstrates excellent cycling stability with a reversible specific capacity of50 mA h g^(-1) keeping over 3700 cycles at 1.0 A g^(-1).In situ XRD,ex situ TEM characterization,and theoretical calculations(DFT) further confirm the reversibility of sodium storage in MoSeTe/N,F@C anode materials during electrochemical reactions,highlighting their potential for widespread practical application.This work provides new insights into the promising utilization of advanced transition metal dichalcogenides as anode materials for Na^(+)-based energy storage devices.展开更多
Landslide displacement prediction can enhance the efficacy of landslide monitoring system,and the prediction of the periodic displacement is particularly challenging.In the previous studies,static regression models(e....Landslide displacement prediction can enhance the efficacy of landslide monitoring system,and the prediction of the periodic displacement is particularly challenging.In the previous studies,static regression models(e.g.,support vector machine(SVM))were mostly used for predicting the periodic displacement.These models may have bad performances,when the dynamic features of landslide triggers are incorporated.This paper proposes a method for predicting the landslide displacement in a dynamic manner,based on the gated recurrent unit(GRU)neural network and complete ensemble empirical decomposition with adaptive noise(CEEMDAN).The CEEMDAN is used to decompose the training data,and the GRU is subsequently used for predicting the periodic displacement.Implementation procedures of the proposed method were illustrated by a case study in the Caojiatuo landslide area,and SVM was also adopted for the periodic displacement prediction.This case study shows that the predictors obtained by SVM are inaccurate,as the landslide displacement is in a pronouncedly step-wise manner.By contrast,the accuracy can be significantly improved using the dynamic predictive method.This paper reveals the significance of capturing the dynamic features of the inputs in the training process,when the machine learning models are adopted to predict the landslide displacement.展开更多
Metastasis is the main cause of cancer mortality. One of the initiating events of cancer metastasis of epithelial tumors is epithelial-to-mesenchymal transition(EMT), during which cells dedifferentiate from a relative...Metastasis is the main cause of cancer mortality. One of the initiating events of cancer metastasis of epithelial tumors is epithelial-to-mesenchymal transition(EMT), during which cells dedifferentiate from a relatively rigid cell structure/morphology to a flexible and changeable structure/morphology often associated with mesenchymal cells. The presence of EMT in human epithelial tumors is reflected by the increased expression of genes and levels of proteins that are preferentially present in mesenchymal cells. The combined presence of these genes forms the basis of mesenchymal gene signatures, which are the foundation for classifying a mesenchymal subtype of tumors. Indeed, tumor classification schemes that use clustering analysis of large genomic characterizations, like The Cancer Genome Atlas(TCGA), have defined mesenchymal subtype in a number of cancer types, such as high-grade serous ovarian cancer and glioblastoma. However, recent analyses have shown that gene expression-based classifications of mesenchymal subtypes often do not associate with poor survival. This "paradox" can be ameliorated using integrated analysis that combines multiple data types. We recently found that integrating m RNA and micro RNA(mi RNA) data revealed an integrated mesenchymal subtype that is consistently associated with poor survival in multiple cohorts of patients with serous ovarian cancer. This network consists of 8 major mi RNAs and 214 m RNAs. Among the 8 mi RNAs, 4 are known to be regulators of EMT. This review provides a summary of these 8 mi RNAs, which were associated with the integrated mesenchymal subtype of serous ovarian cancer.展开更多
Structural characteristics and proton binding properties of sub-fractions（FA3–FA13） of fulvic acid（FA）, eluted stepwise by pyrophosphate buffer were examined by use of fluorescence titration combined with fluores...Structural characteristics and proton binding properties of sub-fractions（FA3–FA13） of fulvic acid（FA）, eluted stepwise by pyrophosphate buffer were examined by use of fluorescence titration combined with fluorescence regional integration（FRI） and differential fluorescence spectroscopy（DFS）. Humic-like（H-L） and fulvic-like（F-L） materials, which accounted for more than 80% of fluorescence response, were dominant in five sub-fractions of FA. Based on FRI analysis, except the response of F-L materials in FA9 and FA13, maximum changes in percent fluorescence response were less than 10% as pH was increased from 2.5 to 11.5.Contents of carboxylic and phenolic groups were compared for fluorescence peaks of FA sub-fractions based on pH-dependent fluorescence derived from DFS. Static quenching was the dominant mechanism for binding of protons by FA sub-fractions. Dissociation constants（p Ka） were calculated by use of results of DFS and the modified Stern-Volmer relationship. The p Kaof H-L, F-L, tryptophan-like and tyrosine-like materials of FA subfractions exhibited ranges of 3.17–4.06, 3.12–3.97, 4.14–4.45 and 4.25–4.76, respectively, for acidic pHs. At basic pHs, values of p Ka for corresponding materials were in ranges of 9.71–10.24, 9.62–10.99, 9.67–10.31 and 9.33–10.28, respectively. At acidic pH, protein-like（P-L）materials had greater affinities for protons than did either H-L or F-L materials. The dicarboxylic and phenolic groups were likely predominant sites of protonation for both H-L and F-L materials at both acidic and basic pHs. Amino acid groups were significant factors during proton binding to protein-like materials of FA sub-fractions at basic pH.展开更多
The friction behavior of resin-impregnated and non-impregnated graphite sliding against a cemented carbide in dry, oil, and water environments using a ring–ring configuration was investigated. Friction coefficients w...The friction behavior of resin-impregnated and non-impregnated graphite sliding against a cemented carbide in dry, oil, and water environments using a ring–ring configuration was investigated. Friction coefficients were recorded at various speeds. The results showed that the impregnated graphite exhibited much better friction properties under water or oil lubrication than non-impregnated graphite, and the impregnated graphite could remain in the stable friction regime under high pressure × velocity（pv）.Based on scanning electron microscopy and Raman spectroscopy analyses, the different characteristics between impregnated and non-impregnated graphite were able to be attributed to the structure of the graphite and wettability of the lubricants.展开更多
Bio-sourced nylon 69,one of promising engineering plastics,has a great potential in developing sustainable technology and various commercial applications.Isothermal and nonisothermal crystallization kinetics of nylon ...Bio-sourced nylon 69,one of promising engineering plastics,has a great potential in developing sustainable technology and various commercial applications.Isothermal and nonisothermal crystallization kinetics of nylon 69 is a base to optimize the process conditions and establish the structure–property correlations for nylon 69,and it is also highly bene ficial for successful applications of nylon products in industry.Isothermal and nonisothermal crystallization kinetics has been investigated by differential scanning calorimetry for nylon 69,bio-sourced even–odd nylon.The isothermal crystallization kinetics has been analyzed by the Avrami equation,the calculated Avrami exponent at various crystallization temperatures falls into the range of 2.28 and 2.86.In addition,the Avrami equation modi fied by Jeziorny and the equation suggested by Mo have been adopted to study the nonisothermal crystallization.The activation energies for isothermal and nonisothermal crystallization have also been determined.The study demonstrates that the crystallization model of nylon 69 might be a twodimensional(circular)growth at both isothermal and nonisothermal crystallization conditions.Furthermore,the value of the crystallization rate parameter(K)decreases signi ficantly but the crystallization half-time(t1/2)increases with the increase of the isothermal crystallization temperature.To nonisothermal crystallization,the crystallization rate increases as the cooling rate increases according to the analysis of Jeziorny's theory.The results of Mo's theory suggest that a faster cooling rate is required to reach a higher relative degree of crystallinity in a unit of time,and crystallization rate decreases when the relative degree of crystallinity increases at nonisothermal crystallization conditions.展开更多
To consider fiber random distribution at the microscale for the multiscale model based on the micro-mechanics failure(MMF)theory,clustering method is used for the extraction of amplification factors.As the clustering ...To consider fiber random distribution at the microscale for the multiscale model based on the micro-mechanics failure(MMF)theory,clustering method is used for the extraction of amplification factors.As the clustering method is a kind of unsupervised machine learning method,the elements with similar mechanical behavior under external loading can be included in a cluster automatically at the microscale.With this modification,the fiber random distribution model can be used for multiscale damage analysis in the framework of MMF theory.To validate the modified multiscale analysis method,progressive damage analysis of a kind of 2D twill woven composites is conducted based on different microscale models.The stress values for microscale models with fiber hexagonal and random distribution patterns are compared first.Much higher stress concentration is generated in the fiber random distribution model due to the smaller inter-fiber distance especially under longitudinal shear loading.The obtained cluster distribution results exhibit the characters of the stress distribution in the two microscale models.Thereafter,tensile and compressive responses of the 2D twill woven composite are predicted with the modified multiscale analysis method and accuracy of the method is verified through comparison with published experimental results.From the simulation results,it can be found that the matrix damage initiation from the model based on the fiber random distribution model is premature compared with that from the model based on the fiber hexagonal distribution model.Besides,under tensile loading,the damage all initiates from the fill tows and propagates to the wrap tows.However,under compressive loading,the matrix damage initiates from the wrap tows in the model based on the fiber random distribution model.展开更多
Although their cost-effectiveness and intrinsic safety,aqueous zinc-ion batteries suffer from notorious side reactions including hydrogen evolution reaction,Zn corrosion and passivation,and Zn dendrite formation on th...Although their cost-effectiveness and intrinsic safety,aqueous zinc-ion batteries suffer from notorious side reactions including hydrogen evolution reaction,Zn corrosion and passivation,and Zn dendrite formation on the anode.Despite numerous strategies to alleviate these side reactions have been demonstrated,they can only provide limited performance improvement from a single aspect.Herein,a triple-functional additive with trace amounts,ammonium hydroxide,was demonstrated to comprehensively protect zinc anodes.The results show that the shift of electrolyte pH from 4.1 to 5.2 lowers the HER potential and encourages the in situ formation of a uniform ZHS-based solid electrolyte interphase on Zn anodes.Moreover,cationic NH^(4+)can preferentially adsorb on the Zn anode surface to shield the“tip effect”and homogenize the electric field.Benefitting from this comprehensive protection,dendrite-free Zn deposition and highly reversible Zn plating/stripping behaviors were realized.Besides,improved electrochemical performances can also be achieved in Zn//MnO_(2)full cells by taking the advantages of this triple-functional additive.This work provides a new strategy for stabilizing Zn anodes from a comprehensive perspective.展开更多
To illuminate the migration and transformation of selenium(Se)in the igneous rock-soil-rice system,285 pairs of rhizosphere soil and rice samples were collected from the granitoid and basalt areas in Hainan Province,S...To illuminate the migration and transformation of selenium(Se)in the igneous rock-soil-rice system,285 pairs of rhizosphere soil and rice samples were collected from the granitoid and basalt areas in Hainan Province,South China.The contents of Se in soils derived from granitoid and basalt are,respectively,0.19±0.12 mg/kg and 0.34±0.39 mg/kg,which are much higher than Se contents in granitoid and basalt.Selenium shows remarkable enrichment from granitoid and basalt to soils.The mobile fraction of Se in soils derived from granitoid is 0.0100±0.0034 mg/kg,which is significantly higher than that of basalt(0.0058±0.0039 mg/kg).Although soil derived from basalt shows higher Se contents,Se contents in rice samples,mobile fractions of Se in soils,and biological concentration factor(BCF)is similar or even lower than that from granitoid.Basalt consist of calcic plagioclase and pyroxene,and are much richer in Fe,Al,and Ca than granitoid.Correspondingly,the basalt-derived soils have higher goethite,hematite,kaolinite,cation exchange capacity(CEC)content,and higher p H than the granitoid-derived soils,which result in higher adsorption capacity for Se and relatively lower Se bioavailability.Soils derived from granitoid and basalt in tropical regions are beneficial to produce Se-rich rice.展开更多
基金supported by the Basic Research and Talent Cultivation Program of Zhangjiakou City(No.2511028A).
文摘Cancer is a major threat to human health worldwide.Colorectal cancer(CRC),a highly prevalent malignant tumor,poses a significant public health challenge.Therefore,the identification of effective biomarkers is of great significance[1].The NFKBIE gene encodes an inhibitor of nuclear factorκBε(IkBε).IκBε,a key regulator of the NF-κB signaling pathway,is closely associated with tumorigenesis.However,their roles in CRC remain unclear[2].Pan-cancer research is crucial for accelerating the identification of biomarkers and translational medical research,as it can reveal molecular commonalities and differences among different tumor types[3].
基金supported by National Natural Science Foundation of China (52201107)Research Program of Chongqing Municipal Education Commission (KJQN202201151)Natural Science Foundation of Chongqing (CSTB2023NSCQ-MSX0067).
文摘Mechanism of discontinuous precipitation(DP) in AZ80 alloy was investigated by phase-orientation correlated characterization.The results show DPs nucleate by turning the original grain boundaries(GBs) as reaction front(RF),and further driving the RF to realize their growth.The DPs regions retained the same orientations as their parent grains.The misorientation angle and rotation axis of RFs had strong influence on DPs nucleation.The low-angle GBs,twin boundaries(TBs) and the GBs with specific misorientation axis which are known as low energy and low mobility GBs can hardly initiate DPs.In addition,the TBs had a strong ability to inhibit the growth of DPs,but it should be noticed that the growth of DPs cannot be totally inhibited by TBs.DPs can engulf the twins when the growth direction is approximately parallel to the long axis of TBs.The inhibition behavior is related to the distribution of Al solute atoms near the RF,boundary interactions of the TBs and twin tips with the RF,and the morphology of the continuous precipitations within the twins.
基金financially supported by the Cultivation Project of CQUT for Research and Innovation Group(No.2023TDZ006)the Graduate Student Innovation Program of CQUT(No.CYS23648)the Postdoctoral Science Foundation of China(No.2021M690174)。
文摘A high-purity Ti sheet with dense preexisting twins(introduced by 10%cold rolling)was subjected to isochronal annealing at 500–800°C for 1 h and isothermal annealing at 600°C for 0.17–100 h,respectively.By mainly utilizing electron backscatter diffraction(EBSD)and electron channel contrast(ECC)imaging techniques,the microstructure and texture evolution during the isochronal and isothermal annealing were investigated systematically.Results show that recrystallization nuclei appear in the specimen annealed at 600°C for 1 h.In contrast,recrystallization cannot be initiated for those annealed at lower temperatures or for a shorter time.With the increase in temperature or time,the fraction of the recrystallized structure increases with gradual grain coarsening.Nearly complete recrystallization is reached after 800°C-1 h or 600°C-100 h annealing.Due to the distribution heterogeneity of microstructure and stored energy induced by the dense preexisting twins,recrystallization nucleation preferentially occurs in some specific regions(twin-twin or twin-grain boundary junctions).Then,they selectively consume twin lamellar structures,leading to non-uniform grain growth.It is demonstrated that the recrystallization nucleation is dominated by the strain-induced boundary migration mechanism,allowing scattered texture components corresponding to the twin lamellar structures to be gradually encroached by those untwinned structures with the initial bimodal basal texture(BBT).Eventually,a strong BBT is always obtained after sufficient recrystallization.
文摘Objective:To analyze the application value of a non-catheter tampon in abdominal hysterectomy,providing a reference for related research.Methods:A total of 100 patients were included in this study,with data collected between January 4,2022,and January 4,2024.The patients were divided into two groups:the new group and the traditional group,each comprising 50 patients.Results:Compared with the traditional group,the new group demonstrated significantly lower intraoperative blood loss(P<0.05).Additionally,the incidence of complications,operation time,hospital stay,time required to resume normal activities,and postoperative VAS scores were all significantly lower in the new group(P<0.05).Conclusion:The application of a non-catheter tampon during abdominal hysterectomy yields satisfactory results.This approach is worthy of further clinical promotion and application.
基金supported by the National Natural Science Foundation of China,Nos.82201621(to LS),31930048(to QY)and 81720108016(to QY),and 81971225(to CG)the Key Research and Development Project of Shaanxi Province,No.2022SF-189(to XS)the Tangdu Hospital Supporting Foundation,Nos.2021ZTXM-006(to LS)and 2021JSZH-006(to CG)。
文摘Transferring healthy and functional mitochondria to the lateral ventricles confers neuroprotection in a rat model of ischemia-reperfusion injury.Autologous mitochondrial transplantation is also beneficial in pediatric patients with cardiac ischemia-reperfusion injury.Thus,transplantation of functional exogenous mitochondria may be a promising therapeutic approach for ischemic disease.To explore the neuroprotective effect of mitochondria transplantation and determine the underlying mechanism in ischemic stroke,in this study we established a photo-thrombosis-induced mouse model of focal ischemia and administered freshly isolated mitochondria via the tail vein or to the injury site(in situ).Animal behavior tests,immunofluorescence staining,2,3,5-triphenyltetrazolium chloride(TTC)staining,mRNA-seq,and western blotting were used to assess mouse anxiety and memory,cortical infarct area,pyroptosis,and neurogenesis,respectively.Using bioinformatics analysis,western blotting,co-immunoprecipitation,and mass spectroscopy,we identified S100 calcium binding protein A9(S100A9)as a potential regulator of mitochondrial function and determined its possible interacting proteins.Interactions between exogenous and endogenous mitochondria,as well as the effect of exogenous mitochondria on recipient microglia,were assessed in vitro.Our data showed that:(1)mitochondrial transplantation markedly reduced mortality and improved emotional and cognitive function,as well as reducing infarct area,inhibiting pyroptosis,and promoting cortical neurogenesis;(2)microglial expression of S100A9 was markedly increased by ischemic injury and regulated mitochondrial function;(3)in vitro,exogenous mitochondria enhanced mitochondrial function,reduced redox stress,and regulated microglial polarization and pyroptosis by fusing with endogenous mitochondria;and(4)S100A9 promoted internalization of exogenous mitochondria by the microglia,thereby amplifying their pro-proliferation and anti-inflammatory effects.Taken together,our findings show that mitochondrial transplantation protects against the deleterious effects of ischemic stroke by suppressing pyroptosis and promoting neurogenesis,and that S100A9 plays a vital role in promoting internalization of exogenous mitochondria.
基金supported by the National Key Research and Development Program of China(Grant No.2018YFD1000600)the National Natural Science Foundation of China(Grant No.32070376)。
文摘In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino acids.The tea plant(Camellia sinensis)is a perennial evergreen with a relatively high level of amino acids.However,systematic identification and molecular characterization of the LHT gene family has rarely been reported in tea plants.In this study,22 CsLHTs were identified from the‘Shuchazao’genome and classified into two groups.The modeled three-dimensional structure and the conserved domains presented a high similarity among the LHTs proteins.Moreover,it was predicted that a few genes were conserved through the analysis of the physiochemical characters,structures and cis-elements in promoters.The expression patterns in tea plants revealed that CsLHT7 was mainly expressed in the roots,and CsLHT4 and CsLHT11 exhibited relatively high expression in both the roots and leaves.Moreover,the expression of all three genes could be induced by organic nitrogen.Additionally,heterogeneous expression of CsLHT4,CsLHT7 and CsLHT11 in Arabidopsis thaliana decreased the aerial parts biomass compared with that in WT plants while significantly increased the rosette biomass only for CsLHT11transgenic plants versus WT plants.Overall,our results provide fundamental information about CsLHTs and potential genes in N utilization for further analysis in tea plants.
文摘BACKGROUND The mucosal barrier's immune-brain interactions,pivotal for neural development and function,are increasingly recognized for their potential causal and therapeutic relevance to irritable bowel syndrome(IBS).Prior studies linking immune inflammation with IBS have been inconsistent.To further elucidate this relationship,we conducted a Mendelian randomization(MR)analysis of 731 immune cell markers to dissect the influence of various immune phenotypes on IBS.Our goal was to deepen our understanding of the disrupted brain-gut axis in IBS and to identify novel therapeutic targets.AIM To leverage publicly available data to perform MR analysis on 731 immune cell markers and explore their impact on IBS.We aimed to uncover immunophenotypic associations with IBS that could inform future drug development and therapeutic strategies.METHODS We performed a comprehensive two-sample MR analysis to evaluate the causal relationship between immune cell markers and IBS.By utilizing genetic data from public databases,we examined the causal associations between 731 immune cell markers,encompassing median fluorescence intensity,relative cell abundance,absolute cell count,and morphological parameters,with IBS susceptibility.Sensitivity analyses were conducted to validate our findings and address potential heterogeneity and pleiotropy.RESULTS Bidirectional false discovery rate correction indicated no significant influence of IBS on immunophenotypes.However,our analysis revealed a causal impact of IBS on 30 out of 731 immune phenotypes(P<0.05).Nine immune phenotypes demonstrated a protective effect against IBS[inverse variance weighting(IVW)<0.05,odd ratio(OR)<1],while 21 others were associated with an increased risk of IBS onset(IVW≥0.05,OR≥1).CONCLUSION Our findings underscore a substantial genetic correlation between immune cell phenotypes and IBS,providing valuable insights into the pathophysiology of the condition.These results pave the way for the development of more precise biomarkers and targeted therapies for IBS.Furthermore,this research enriches our comprehension of immune cell roles in IBS pathogenesis,offering a foundation for more effective,personalized treatment approaches.These advancements hold promise for improving IBS patient quality of life and reducing the disease burden on individuals and their families.
基金supported by Research Program of Chongqing Municipal Education Commission(KJQN201901127)University Innovation Research Group of Chongqing(CXQT20023)+2 种基金Natural Science Foundation of Chongqing(cstc2021ycjh-bgzxm0184)support by the Research Program of Chongqing Municipal Education Commission(KJQN202201151)National Natural Science Foundation of China(52201107).
文摘LZ91 Mg-Li alloy plates with three types of initial texture were rolled by 70%reduction at both room temperature and 200℃to explore the rolling texture formation ofα-Mg phase.The results showed that the rolling texture is largely affected by the initial texture.All the samples exhibited two main texture components as RD-split double peaks texture and TD-split double peaks texture after large strain rolling.The intensity of the two texture components was strongly influenced by the initial orientation and rolling temperature.Extension twinning altered the large-split non-basal orientation to a near basal one at low rolling strain.The basal orientation induced by twinning is unstable,which finally transmitted to the RD-split texture.The strong TD-split texture formed due to slip-induced orientation transition from its initial orientation.The competition between prismatic and basal slip determined the intensity and tilt angle of the TD-split texture.By increasing the rolling temperature,the TD-split texture component was enhanced in all three samples.Limitation of extension twinning behavior and the promotion of prismatic slip at elevated temperature are the main reasons for the difference in hot and cold rolling texture.
基金supported by the National Natural Science Foundation of China(No.52002320,and 51972267)the China Postdoctoral Science Foundation(No.2022M712574)+3 种基金the Science Foundation of Shaanxi Province(2022GD-TSLD-18,No.2023-JCZD-03)Natural Science Foundation of Shaanxi Province(No.2022GY-372,2021GY-153)Industrial Projects Foundation of Ankang Science and Technology Bureau(No.AK2020-GY02-2)the Platform Construction Projects and Technology Service Teams of Ankang University(No.2021AYPT12 and 2022TD07)。
文摘Sodium-ion batteries(SIBs) and hybrid capacitors(SIHCs) have garnered significant attention in energy storage due to their inherent advantages,including high energy density,cost-effectiveness,and enhanced safety.However,developing high-performance anode materials to improve sodium storage performa nce still remains a major challenge.Here,a facile one-pot method has been developed to fabricate a hybrid of MoSeTe nanosheets implanted within the N,F co-doped honeycomb carbon skeleton(MoSeTe/N,F@C).Experimental results demonstrate that the incorporation of large-sized Te atoms into MoSeTe nanosheets enlarges the layer spacing and creates abundant anion vacancies,which effectively facilitate the insertion/extraction of Na^(+) and provide numerous ion adsorption sites for rapid surface capacitive behavior.Additionally,the heteroatoms N,F co-doped honeycomb carbon skeleton with a highly conductive network can restrain the volume expansion and boost reaction kinetics within the electrode.As anticipated,the MoSeTe/N,F@C anode exhibits high reversible capacities along with exceptional cycle stability.When coupled with Na_(3)V_(2)(PO_(4))_(3)@C(NVPF@C) to form SIB full cells,the anode delivers a reversible specific capacity of 126 mA h g^(-1) after 100 cycles at 0.1 A g^(-1).Furthermore,when combined with AC to form SIHC full cells,the anode demonstrates excellent cycling stability with a reversible specific capacity of50 mA h g^(-1) keeping over 3700 cycles at 1.0 A g^(-1).In situ XRD,ex situ TEM characterization,and theoretical calculations(DFT) further confirm the reversibility of sodium storage in MoSeTe/N,F@C anode materials during electrochemical reactions,highlighting their potential for widespread practical application.This work provides new insights into the promising utilization of advanced transition metal dichalcogenides as anode materials for Na^(+)-based energy storage devices.
基金The authors appreciate the financial support provided by the Natural Science Foundation of China(No.41807294)This study was also financially supported by China Geological Survey Project(Nos.DD20190716 and 0001212020CC60002)。
文摘Landslide displacement prediction can enhance the efficacy of landslide monitoring system,and the prediction of the periodic displacement is particularly challenging.In the previous studies,static regression models(e.g.,support vector machine(SVM))were mostly used for predicting the periodic displacement.These models may have bad performances,when the dynamic features of landslide triggers are incorporated.This paper proposes a method for predicting the landslide displacement in a dynamic manner,based on the gated recurrent unit(GRU)neural network and complete ensemble empirical decomposition with adaptive noise(CEEMDAN).The CEEMDAN is used to decompose the training data,and the GRU is subsequently used for predicting the periodic displacement.Implementation procedures of the proposed method were illustrated by a case study in the Caojiatuo landslide area,and SVM was also adopted for the periodic displacement prediction.This case study shows that the predictors obtained by SVM are inaccurate,as the landslide displacement is in a pronouncedly step-wise manner.By contrast,the accuracy can be significantly improved using the dynamic predictive method.This paper reveals the significance of capturing the dynamic features of the inputs in the training process,when the machine learning models are adopted to predict the landslide displacement.
基金supported by the U.S. National Institutes of Health grants (U24 CA143835 to IS and WZ, P50 CA083639 and P50 CA098258 to AKS)MD Anderson support grant (CA016672) to WZ+6 种基金a grant from the Blanton-Davis Ovarian Cancer Research Program to WZgrants from the Program for Changjiang Scholars, Innovative Research Team in University (PCSIRT) in Chinathe National Key Scientifi c and Technological Project (2011ZX0 9307-001-04)Tianjin Science and Technology Committee Foundation (09ZCZDSF04700) to KCa grant from National Nature Science Foundation of China (#81201651) to YSa grant from Fondazione CARIPLO (2013-0865) to DMthe A. Lavoy Moore Endowment Fund to YS and DY
文摘Metastasis is the main cause of cancer mortality. One of the initiating events of cancer metastasis of epithelial tumors is epithelial-to-mesenchymal transition(EMT), during which cells dedifferentiate from a relatively rigid cell structure/morphology to a flexible and changeable structure/morphology often associated with mesenchymal cells. The presence of EMT in human epithelial tumors is reflected by the increased expression of genes and levels of proteins that are preferentially present in mesenchymal cells. The combined presence of these genes forms the basis of mesenchymal gene signatures, which are the foundation for classifying a mesenchymal subtype of tumors. Indeed, tumor classification schemes that use clustering analysis of large genomic characterizations, like The Cancer Genome Atlas(TCGA), have defined mesenchymal subtype in a number of cancer types, such as high-grade serous ovarian cancer and glioblastoma. However, recent analyses have shown that gene expression-based classifications of mesenchymal subtypes often do not associate with poor survival. This "paradox" can be ameliorated using integrated analysis that combines multiple data types. We recently found that integrating m RNA and micro RNA(mi RNA) data revealed an integrated mesenchymal subtype that is consistently associated with poor survival in multiple cohorts of patients with serous ovarian cancer. This network consists of 8 major mi RNAs and 214 m RNAs. Among the 8 mi RNAs, 4 are known to be regulators of EMT. This review provides a summary of these 8 mi RNAs, which were associated with the integrated mesenchymal subtype of serous ovarian cancer.
基金supported by the National Natural Science Foundation of China(Nos.41173084,41521003,41573130,41630645,41703115 and 41503104)the Beijing Natural Science Foundation(No.8162044)the Canada Research Chair program,Einstein Professor Program of the Chinese Academy of Sciences,and the High Level Foreign Experts Program(#GDT20143200016)
文摘Structural characteristics and proton binding properties of sub-fractions（FA3–FA13） of fulvic acid（FA）, eluted stepwise by pyrophosphate buffer were examined by use of fluorescence titration combined with fluorescence regional integration（FRI） and differential fluorescence spectroscopy（DFS）. Humic-like（H-L） and fulvic-like（F-L） materials, which accounted for more than 80% of fluorescence response, were dominant in five sub-fractions of FA. Based on FRI analysis, except the response of F-L materials in FA9 and FA13, maximum changes in percent fluorescence response were less than 10% as pH was increased from 2.5 to 11.5.Contents of carboxylic and phenolic groups were compared for fluorescence peaks of FA sub-fractions based on pH-dependent fluorescence derived from DFS. Static quenching was the dominant mechanism for binding of protons by FA sub-fractions. Dissociation constants（p Ka） were calculated by use of results of DFS and the modified Stern-Volmer relationship. The p Kaof H-L, F-L, tryptophan-like and tyrosine-like materials of FA subfractions exhibited ranges of 3.17–4.06, 3.12–3.97, 4.14–4.45 and 4.25–4.76, respectively, for acidic pHs. At basic pHs, values of p Ka for corresponding materials were in ranges of 9.71–10.24, 9.62–10.99, 9.67–10.31 and 9.33–10.28, respectively. At acidic pH, protein-like（P-L）materials had greater affinities for protons than did either H-L or F-L materials. The dicarboxylic and phenolic groups were likely predominant sites of protonation for both H-L and F-L materials at both acidic and basic pHs. Amino acid groups were significant factors during proton binding to protein-like materials of FA sub-fractions at basic pH.
基金supported by the National Basic Research Program of China (973) (Grant No. 2015CB057303)the National Natural Science Foundation of China (Grant No. 51275268)
文摘The friction behavior of resin-impregnated and non-impregnated graphite sliding against a cemented carbide in dry, oil, and water environments using a ring–ring configuration was investigated. Friction coefficients were recorded at various speeds. The results showed that the impregnated graphite exhibited much better friction properties under water or oil lubrication than non-impregnated graphite, and the impregnated graphite could remain in the stable friction regime under high pressure × velocity（pv）.Based on scanning electron microscopy and Raman spectroscopy analyses, the different characteristics between impregnated and non-impregnated graphite were able to be attributed to the structure of the graphite and wettability of the lubricants.
基金Supported by the Natural Science Foundation of Zhejiang Province(LY15B060006)the National Natural Science Foundation of China(21104066)the Zhejiang Province Public Technology Research and Industrial Grant(2012C21078)
文摘Bio-sourced nylon 69,one of promising engineering plastics,has a great potential in developing sustainable technology and various commercial applications.Isothermal and nonisothermal crystallization kinetics of nylon 69 is a base to optimize the process conditions and establish the structure–property correlations for nylon 69,and it is also highly bene ficial for successful applications of nylon products in industry.Isothermal and nonisothermal crystallization kinetics has been investigated by differential scanning calorimetry for nylon 69,bio-sourced even–odd nylon.The isothermal crystallization kinetics has been analyzed by the Avrami equation,the calculated Avrami exponent at various crystallization temperatures falls into the range of 2.28 and 2.86.In addition,the Avrami equation modi fied by Jeziorny and the equation suggested by Mo have been adopted to study the nonisothermal crystallization.The activation energies for isothermal and nonisothermal crystallization have also been determined.The study demonstrates that the crystallization model of nylon 69 might be a twodimensional(circular)growth at both isothermal and nonisothermal crystallization conditions.Furthermore,the value of the crystallization rate parameter(K)decreases signi ficantly but the crystallization half-time(t1/2)increases with the increase of the isothermal crystallization temperature.To nonisothermal crystallization,the crystallization rate increases as the cooling rate increases according to the analysis of Jeziorny's theory.The results of Mo's theory suggest that a faster cooling rate is required to reach a higher relative degree of crystallinity in a unit of time,and crystallization rate decreases when the relative degree of crystallinity increases at nonisothermal crystallization conditions.
基金the support of the National Natural Science Foundation of China(No.11572086)the Fundamental Research Funds for the Central Universities+2 种基金the Scientific Research Innovation Program of Jiangsu Province College of China(No.KYLX16_0185)the Scientific Research Foundation of Graduate School of Southeast University of China(No.YBJJ1760)the China Scholarship Council of China(No.201706090076)。
文摘To consider fiber random distribution at the microscale for the multiscale model based on the micro-mechanics failure(MMF)theory,clustering method is used for the extraction of amplification factors.As the clustering method is a kind of unsupervised machine learning method,the elements with similar mechanical behavior under external loading can be included in a cluster automatically at the microscale.With this modification,the fiber random distribution model can be used for multiscale damage analysis in the framework of MMF theory.To validate the modified multiscale analysis method,progressive damage analysis of a kind of 2D twill woven composites is conducted based on different microscale models.The stress values for microscale models with fiber hexagonal and random distribution patterns are compared first.Much higher stress concentration is generated in the fiber random distribution model due to the smaller inter-fiber distance especially under longitudinal shear loading.The obtained cluster distribution results exhibit the characters of the stress distribution in the two microscale models.Thereafter,tensile and compressive responses of the 2D twill woven composite are predicted with the modified multiscale analysis method and accuracy of the method is verified through comparison with published experimental results.From the simulation results,it can be found that the matrix damage initiation from the model based on the fiber random distribution model is premature compared with that from the model based on the fiber hexagonal distribution model.Besides,under tensile loading,the damage all initiates from the fill tows and propagates to the wrap tows.However,under compressive loading,the matrix damage initiates from the wrap tows in the model based on the fiber random distribution model.
基金supported by the National Key Research and Development Program of China(2019YFE0114400)the Guangdong Basic and Applied Basic Research Foundation(2021B1515120005)+7 种基金the National Natural Science Foundation of China(32171721)the Guangdong Basic and Applied Basic Research Foundation(2021B151512000)the Guangzhou Science and Technology Plan Project(202102020262)the State Key Laboratory of Pulp&Paper Engineering(2022C01),the State Key Laboratory of Pulp&Paper Engineering(202208)the Engineering and Physical Sciences Research Council(EPSRCEP/V027433/1EP/V027433/2EP/Y008707/1)。
文摘Although their cost-effectiveness and intrinsic safety,aqueous zinc-ion batteries suffer from notorious side reactions including hydrogen evolution reaction,Zn corrosion and passivation,and Zn dendrite formation on the anode.Despite numerous strategies to alleviate these side reactions have been demonstrated,they can only provide limited performance improvement from a single aspect.Herein,a triple-functional additive with trace amounts,ammonium hydroxide,was demonstrated to comprehensively protect zinc anodes.The results show that the shift of electrolyte pH from 4.1 to 5.2 lowers the HER potential and encourages the in situ formation of a uniform ZHS-based solid electrolyte interphase on Zn anodes.Moreover,cationic NH^(4+)can preferentially adsorb on the Zn anode surface to shield the“tip effect”and homogenize the electric field.Benefitting from this comprehensive protection,dendrite-free Zn deposition and highly reversible Zn plating/stripping behaviors were realized.Besides,improved electrochemical performances can also be achieved in Zn//MnO_(2)full cells by taking the advantages of this triple-functional additive.This work provides a new strategy for stabilizing Zn anodes from a comprehensive perspective.
基金financially supported by the projects of the China Geological Survey(DD20190518,DD20190527)。
文摘To illuminate the migration and transformation of selenium(Se)in the igneous rock-soil-rice system,285 pairs of rhizosphere soil and rice samples were collected from the granitoid and basalt areas in Hainan Province,South China.The contents of Se in soils derived from granitoid and basalt are,respectively,0.19±0.12 mg/kg and 0.34±0.39 mg/kg,which are much higher than Se contents in granitoid and basalt.Selenium shows remarkable enrichment from granitoid and basalt to soils.The mobile fraction of Se in soils derived from granitoid is 0.0100±0.0034 mg/kg,which is significantly higher than that of basalt(0.0058±0.0039 mg/kg).Although soil derived from basalt shows higher Se contents,Se contents in rice samples,mobile fractions of Se in soils,and biological concentration factor(BCF)is similar or even lower than that from granitoid.Basalt consist of calcic plagioclase and pyroxene,and are much richer in Fe,Al,and Ca than granitoid.Correspondingly,the basalt-derived soils have higher goethite,hematite,kaolinite,cation exchange capacity(CEC)content,and higher p H than the granitoid-derived soils,which result in higher adsorption capacity for Se and relatively lower Se bioavailability.Soils derived from granitoid and basalt in tropical regions are beneficial to produce Se-rich rice.
