目的分析辽宁省肉类食物中小肠结肠炎耶尔森菌病原学特征。方法对从肉类食品中分离的小肠结肠炎耶尔森菌进行病原生物学分析、多位点序列分型(multilocus sequence typing,MLST)试验及耐药性检测分析。结果2024年从肉类食品中共检出40株...目的分析辽宁省肉类食物中小肠结肠炎耶尔森菌病原学特征。方法对从肉类食品中分离的小肠结肠炎耶尔森菌进行病原生物学分析、多位点序列分型(multilocus sequence typing,MLST)试验及耐药性检测分析。结果2024年从肉类食品中共检出40株,血清型鉴定出O:8、O:3、O:53种,以O:8型为主;生物型为1A型和3型;毒力基因检测可分为4种型别,主要型别为I型携带foxA;脉冲场凝胶电泳(pluse field gel eletrophoresis,PFGE)分为10组为A~J,A组占比高;MLST共分为26个型别,其中有10个新型,STN16和STN11位于核心位置向四周发散;药物敏感试验对萘啶酸耐药率最高,其次为链霉素,再次为环丙沙星和氨苄西林耐药。结论2024年辽宁省肉类食品中分离的小肠结肠炎耶尔森菌血清型为3种,生物型非常集中,毒力基因以I型为主,序列分型(sequence type,ST)基因型呈多样性发散分布,多重抗生素耐药模式,应重点加强对猪、牛、鸡等肉类食品的监管。展开更多
Autologous bone marrow-derived mesenchymal stem cells(BMSCs)have been shown to promote osteogenesis;however,the effects of allogeneic BMSCs(allo-BMSCs)on bone regeneration remain unclear.Therefore,we explored the bone...Autologous bone marrow-derived mesenchymal stem cells(BMSCs)have been shown to promote osteogenesis;however,the effects of allogeneic BMSCs(allo-BMSCs)on bone regeneration remain unclear.Therefore,we explored the bone regeneration promotion effect of allo-BMSCs in 3D-printed autologous bone particle(ABP)scaffolds.First,we concurrently printed scaffolds with polycaprolactone,ABPs,and allo-BMSCs for appropriate support,providing bioactive factors and seed cells to promote osteogenesis.In vitro studies showed that ABP scaffolds promoted allo-BMSC osteogenic differentiation.In vivo studies revealed that the implantation of scaffolds loaded with ABPs and allo-BMSCs into canine skull defects for nine months promoted osteogenesis.Further experiments suggested that only a small portion of implanted allo-BMSCs survived and differentiated into vascular endothelial cells,chondrocytes,and osteocytes.The implanted allo-BMSCs released stromal cell-derived factor 1 through paracrine signaling to recruit native BMSCs into the defect,promoting bone regeneration.This study contributes to our understanding of allo-BMSCs,providing information relevant to their future application.展开更多
文摘目的分析辽宁省肉类食物中小肠结肠炎耶尔森菌病原学特征。方法对从肉类食品中分离的小肠结肠炎耶尔森菌进行病原生物学分析、多位点序列分型(multilocus sequence typing,MLST)试验及耐药性检测分析。结果2024年从肉类食品中共检出40株,血清型鉴定出O:8、O:3、O:53种,以O:8型为主;生物型为1A型和3型;毒力基因检测可分为4种型别,主要型别为I型携带foxA;脉冲场凝胶电泳(pluse field gel eletrophoresis,PFGE)分为10组为A~J,A组占比高;MLST共分为26个型别,其中有10个新型,STN16和STN11位于核心位置向四周发散;药物敏感试验对萘啶酸耐药率最高,其次为链霉素,再次为环丙沙星和氨苄西林耐药。结论2024年辽宁省肉类食品中分离的小肠结肠炎耶尔森菌血清型为3种,生物型非常集中,毒力基因以I型为主,序列分型(sequence type,ST)基因型呈多样性发散分布,多重抗生素耐药模式,应重点加强对猪、牛、鸡等肉类食品的监管。
基金supported by the Science and Technology Development Fund of the Fourth Military Medical University(No.2016XB051)the Military Medical Promotion Plan of the Fourth Military Medical University(No.2016TSA-005)+2 种基金the Science and Technology Program of Guangzhou(No.201604040002)the Youth Development Project of Air Force Medical University(No.21QNPY072)the Xijing Hospital Booster Program(No.XJZT24CZ10).
文摘Autologous bone marrow-derived mesenchymal stem cells(BMSCs)have been shown to promote osteogenesis;however,the effects of allogeneic BMSCs(allo-BMSCs)on bone regeneration remain unclear.Therefore,we explored the bone regeneration promotion effect of allo-BMSCs in 3D-printed autologous bone particle(ABP)scaffolds.First,we concurrently printed scaffolds with polycaprolactone,ABPs,and allo-BMSCs for appropriate support,providing bioactive factors and seed cells to promote osteogenesis.In vitro studies showed that ABP scaffolds promoted allo-BMSC osteogenic differentiation.In vivo studies revealed that the implantation of scaffolds loaded with ABPs and allo-BMSCs into canine skull defects for nine months promoted osteogenesis.Further experiments suggested that only a small portion of implanted allo-BMSCs survived and differentiated into vascular endothelial cells,chondrocytes,and osteocytes.The implanted allo-BMSCs released stromal cell-derived factor 1 through paracrine signaling to recruit native BMSCs into the defect,promoting bone regeneration.This study contributes to our understanding of allo-BMSCs,providing information relevant to their future application.
