Ferroptosis has emerged as a significant pathway in various pathological conditions.Studying the effects of inhibiting ferroptosis on liver injury is instrumental in gaining a deeper understanding of the mechanisms.Th...Ferroptosis has emerged as a significant pathway in various pathological conditions.Studying the effects of inhibiting ferroptosis on liver injury is instrumental in gaining a deeper understanding of the mechanisms.This study the design and synthesis of a multi-channel near-infrared emitting fluorescent probe TXVQ.When the probe TXVQ responds to HSO_(3)^(-),the fluorescence intensity at 500 nm of TXVQ increases with the addition of HSO_(3)^(-).As the concentration of H_(2)O_(2) increases,the fluorescence intensity of TXVQ at 600 nm is enhanced.Concurrently,as viscosity rises,the fluorescence intensity of the probe TXVQ at 725 nm will gradually increase.The probe TXVQ,with its ability to monitor HSO_(3)^(-),H_(2)O_(2) and viscosity through three distinct fluorescent channels,is advantageous for its application in the biological field.Subsequently,cellular experiments have demonstrated that the probe TXVQ can monitor changes in intracellular HSO_(3)^(-),H_(2)O_(2) and viscosity.At the cellular level,it is found that inhibiting ferroptosis had no alleviating effect on drug-induced liver injury(DILI),but it had a certain alleviating effect on acute kidney injury(AKI).In a murine model,the effects of ferroptosis inhibition on DILI and AKI indicate that inhibiting ferroptosis reduced kidney injury but not liver injury,highlighting its potential in kidney therapeutics.TXVQ can detect various levels of HSO_(3)^(-),H_(2)O_(2) and viscosity through three different fluorescent channels,making it a powerful tool for diagnosing and treating kidney diseases,as well as deepening the understanding of the role of ferroptosis in liver and kidney pathologies.展开更多
基金the financial supports from Scientific and Technological Key Project in Henan Province(No.22170015)Development Plan of the Youth Innovation Team in Colleges and Universities of Shandong Province(No.2023KJ219)+1 种基金Zhengzhou University(No.32211807)Henan Provincial Science and Technology Research Project(No.JC21253010)。
文摘Ferroptosis has emerged as a significant pathway in various pathological conditions.Studying the effects of inhibiting ferroptosis on liver injury is instrumental in gaining a deeper understanding of the mechanisms.This study the design and synthesis of a multi-channel near-infrared emitting fluorescent probe TXVQ.When the probe TXVQ responds to HSO_(3)^(-),the fluorescence intensity at 500 nm of TXVQ increases with the addition of HSO_(3)^(-).As the concentration of H_(2)O_(2) increases,the fluorescence intensity of TXVQ at 600 nm is enhanced.Concurrently,as viscosity rises,the fluorescence intensity of the probe TXVQ at 725 nm will gradually increase.The probe TXVQ,with its ability to monitor HSO_(3)^(-),H_(2)O_(2) and viscosity through three distinct fluorescent channels,is advantageous for its application in the biological field.Subsequently,cellular experiments have demonstrated that the probe TXVQ can monitor changes in intracellular HSO_(3)^(-),H_(2)O_(2) and viscosity.At the cellular level,it is found that inhibiting ferroptosis had no alleviating effect on drug-induced liver injury(DILI),but it had a certain alleviating effect on acute kidney injury(AKI).In a murine model,the effects of ferroptosis inhibition on DILI and AKI indicate that inhibiting ferroptosis reduced kidney injury but not liver injury,highlighting its potential in kidney therapeutics.TXVQ can detect various levels of HSO_(3)^(-),H_(2)O_(2) and viscosity through three different fluorescent channels,making it a powerful tool for diagnosing and treating kidney diseases,as well as deepening the understanding of the role of ferroptosis in liver and kidney pathologies.
