Common in Fungal Extracellular Membrane(CFEM)domain proteins are considered to be unique to fungi and closely related to pathogenicity.However,the Puccinia striiformis f.sp.tritici(Pst)effector containing the CFEM dom...Common in Fungal Extracellular Membrane(CFEM)domain proteins are considered to be unique to fungi and closely related to pathogenicity.However,the Puccinia striiformis f.sp.tritici(Pst)effector containing the CFEM domain has not been reported.Here,we obtained an effector,PstCFEM1,containing a functional N-terminal signal peptide sequence and the CFEM domain from Pst race CYR31.qRT-PCR assay indicated that the transcript levels of PstCFEM1 were highly induced during the early stages of infection.Overexpression of PstCFEM1 suppressed Pst322(an elicitor-like protein of Pst)-trigged cell death,reactive oxygen species(ROS)accumulation and callose deposition.Host-induced gene silencing(HIGS)experiments showed that knockdown of PstCFEM1 decreased the virulence of Pst,while ROS accumulation in silenced plants increased near the infection site.In addition,wheat containing the PstCFEM1-silenced construct increased resistance to multiple races of Pst.Our data suggest that PstCFEM1 suppresses wheat defense by inhibiting ROS accumulation and contributes to increased virulence of Pst.展开更多
基金supported by the National Key Research and Development Program of China(2021YFD1401003)the National Natural Science Foundation of China(32172381 and 31972224),Key Research and Development Program of Shaanxi(2021ZDLNY01–01)+1 种基金Natural Science Basic Research Program of Shaanxi(2020JZ-13)the 111 Project from the Ministry of Education of China(B07049).
文摘Common in Fungal Extracellular Membrane(CFEM)domain proteins are considered to be unique to fungi and closely related to pathogenicity.However,the Puccinia striiformis f.sp.tritici(Pst)effector containing the CFEM domain has not been reported.Here,we obtained an effector,PstCFEM1,containing a functional N-terminal signal peptide sequence and the CFEM domain from Pst race CYR31.qRT-PCR assay indicated that the transcript levels of PstCFEM1 were highly induced during the early stages of infection.Overexpression of PstCFEM1 suppressed Pst322(an elicitor-like protein of Pst)-trigged cell death,reactive oxygen species(ROS)accumulation and callose deposition.Host-induced gene silencing(HIGS)experiments showed that knockdown of PstCFEM1 decreased the virulence of Pst,while ROS accumulation in silenced plants increased near the infection site.In addition,wheat containing the PstCFEM1-silenced construct increased resistance to multiple races of Pst.Our data suggest that PstCFEM1 suppresses wheat defense by inhibiting ROS accumulation and contributes to increased virulence of Pst.
