Colon cancer is one of the malignant tumors with high morbidity and mortality worldwide,and its etiology is closely related to high levels of hydrogen sulfide(H_(2)S).To date,H_(2)S-activated near-infrared(NIR)fluores...Colon cancer is one of the malignant tumors with high morbidity and mortality worldwide,and its etiology is closely related to high levels of hydrogen sulfide(H_(2)S).To date,H_(2)S-activated near-infrared(NIR)fluorescent(FL)probes with high tumor tropism are still scarce.In this work,we created a new NIR FL probes(Cy-DG)that enables colon cancer targeted imaging and rapid fecal optical analysis by introducing an H_(2)S-recognition moiety and two D-glucosamine fragments on the scaffold of QCy7.Cy-DG exhibits excellent properties,including specific"off-on"response toward H_(2)S,intense NIR emission at 703 nm,large Stokes shift(118 nm),and high sensitivity(limit of detection(LOD),0.48μmol/L).Notably,the presence of D-glucosamine allows Cy-DG to be preferentially taken up by cancer cells.After intravenous administration,Cy-DG was able to efficiently accumulate in a MC38 intestinal cancer model and sensitively detect endogenous H_(2)S in vivo,showing 5.94-fold higher fluorescence intensity in small tumors<5 mm.Furthermore,Cy-DG was successfully used to detect H_(2)S in feces samples from colon cancer-bearing mice.We believe that Cy-DG has great potential as a powerful diagnostic tool for H_(2)S-related disorder and stool examinations in the future.展开更多
Owing to the intrinsic advantages of spatiotemporal selectivity,photothermal theranostics have become the advancing edge of precision medicine for cancer.Developing photothermal transduction agents(PTAs)with near-infr...Owing to the intrinsic advantages of spatiotemporal selectivity,photothermal theranostics have become the advancing edge of precision medicine for cancer.Developing photothermal transduction agents(PTAs)with near-infrared(NIR)absorption,high photothermal conversion efficiency,robust photothermal stability,and good accumulation in tumors,is particularly valuable.Herein,we report a new concept,self-assembly-induced crystallization(SAIC),which can serve as a mechanism that dramatically boosts photothermal behaviors of PTA in NIR region.As a proof of concept,three heptamethine cyanine molecules with internal degrees of freedom(geometry and intramolecular interaction)are designed to fine-tune their crystallinity.Notably,Cy7-TCF-EMBI molecules with rigid and planar skeletons self-assemble into a crystalline state to maximize their packing density and improve the charge transfer,both of which contribute to nonradiative decay for energy dissipation as heat.The high packing density also renders an ideal scaffold for controlling intermolecular interactions to exhibit better photothermal stability,and endows an anisotropic three-dimensional architecture for passive tumor targeting.This“SAIC”strategy may offer a conceptually novel,practically simple but effective approach to unveil the structure–property relationship that could provide some general rules in rational design of PTAs,and paves the way for a next generation of supramolecular medicine for photothermal theranostics.展开更多
基金financial support from the National Natural Science Foundation of China(Nos.22422407,22174078)the Fundamental Research Funds for the Central Universities,Nankai University(No.020-63253156)support from the Doctoral Foundation Program of Henan University of Technology(No.2021BS035)。
文摘Colon cancer is one of the malignant tumors with high morbidity and mortality worldwide,and its etiology is closely related to high levels of hydrogen sulfide(H_(2)S).To date,H_(2)S-activated near-infrared(NIR)fluorescent(FL)probes with high tumor tropism are still scarce.In this work,we created a new NIR FL probes(Cy-DG)that enables colon cancer targeted imaging and rapid fecal optical analysis by introducing an H_(2)S-recognition moiety and two D-glucosamine fragments on the scaffold of QCy7.Cy-DG exhibits excellent properties,including specific"off-on"response toward H_(2)S,intense NIR emission at 703 nm,large Stokes shift(118 nm),and high sensitivity(limit of detection(LOD),0.48μmol/L).Notably,the presence of D-glucosamine allows Cy-DG to be preferentially taken up by cancer cells.After intravenous administration,Cy-DG was able to efficiently accumulate in a MC38 intestinal cancer model and sensitively detect endogenous H_(2)S in vivo,showing 5.94-fold higher fluorescence intensity in small tumors<5 mm.Furthermore,Cy-DG was successfully used to detect H_(2)S in feces samples from colon cancer-bearing mice.We believe that Cy-DG has great potential as a powerful diagnostic tool for H_(2)S-related disorder and stool examinations in the future.
基金National Natural Science Foundation of China,Grant/Award Numbers:22071128,51773107,51690152Taishan Scholars Project of Shandong Province,Grant/Award Number:tsqn201812072+1 种基金Major Scientific and Technological In-novation Projects of key R&D programs in Shandong Province,Grant/Award Number:2019JZZY021007Shan-dong Provincial Natural Science Foundation,Grant/Award Number:ZR2020ZD31。
文摘Owing to the intrinsic advantages of spatiotemporal selectivity,photothermal theranostics have become the advancing edge of precision medicine for cancer.Developing photothermal transduction agents(PTAs)with near-infrared(NIR)absorption,high photothermal conversion efficiency,robust photothermal stability,and good accumulation in tumors,is particularly valuable.Herein,we report a new concept,self-assembly-induced crystallization(SAIC),which can serve as a mechanism that dramatically boosts photothermal behaviors of PTA in NIR region.As a proof of concept,three heptamethine cyanine molecules with internal degrees of freedom(geometry and intramolecular interaction)are designed to fine-tune their crystallinity.Notably,Cy7-TCF-EMBI molecules with rigid and planar skeletons self-assemble into a crystalline state to maximize their packing density and improve the charge transfer,both of which contribute to nonradiative decay for energy dissipation as heat.The high packing density also renders an ideal scaffold for controlling intermolecular interactions to exhibit better photothermal stability,and endows an anisotropic three-dimensional architecture for passive tumor targeting.This“SAIC”strategy may offer a conceptually novel,practically simple but effective approach to unveil the structure–property relationship that could provide some general rules in rational design of PTAs,and paves the way for a next generation of supramolecular medicine for photothermal theranostics.
