Lesion mimic is necrotic lesions on plant leaf or stem in the absence of pathogenic infection, and its exact biological mechanism is varied. By a large-scale screening of our T-DNA mutant population, we identified a m...Lesion mimic is necrotic lesions on plant leaf or stem in the absence of pathogenic infection, and its exact biological mechanism is varied. By a large-scale screening of our T-DNA mutant population, we identified a mutant rice lesion initiation 1 （rlin1）, which was controlled by a single nuclear recessive gene. Map-based cloning revealed that RLIN1 encoded a putative coproporphyrinogen Ⅲ oxidase in tetrapyrrole biosynthesis pathway. Sequencing results showed that a G to T substitution occurred in the second exon of RLIN1 and led to a missense mutation from Asp to Tyr. Ectopic expression of RLIN1 could rescue rlin1 lesion mimic phenotype. Histochemical analysis demonstrated that lesion formation in rlin1 was light-dependent accompanied by reactive oxygen species accumulated. These results suggest that tetrapyrrole participates in lesion formation in rice.展开更多
COMPASS or COMPASS-like is a highly conserved polyprotein complex in eukaryotes that is often involved in methylation of histone H3 lysine 4(H3K4).However,the biological function of this complex in rice(Oryza sativa)i...COMPASS or COMPASS-like is a highly conserved polyprotein complex in eukaryotes that is often involved in methylation of histone H3 lysine 4(H3K4).However,the biological function of this complex in rice(Oryza sativa)is unclear.Here,we report the identifiction of their functions in growth and development.The osashl1osashl2 double mutant shows a dwarf and late-flowering phenotype.Lower expression of Ehd1,OsVIL4,and OsMADS51 in the osashl1 osashl2 double mutant background accompanies a delayed vegetative growth phase and photoperiod-sensitive phase compared with that in wild type.Notably,there is less H3K4mono-,di-and tri-methylation genome-wide in the double mutant,in particular less H3K4 tri-methylation at OsVIL4.Consistent with this result,knockout of OsVIL4 gives rise to a late-flowering phenotype similar to that of the osashl1 osashl2 double mutant,suggesting that OsVIL4 is a target of the COMPASS-like complex.In addition,the expression of key genes in brassinosteroid and gibberellic acid metabolism is altered in the osashl1 osashl2 double mutant,suggesting that the COMPASS-like complex regulates plant growth and development by modulating the levels of these two phytohormones.In summary,we demonstrate that OsASHL1 and OsASHL2 are important for floral transition and plant development.展开更多
基金This work was supported by grants from the Ministry of Science and Technology of China(No.2009CB118506)the National Natural Science Foundation of China(Nos. 30825029 and 30621001)
文摘Lesion mimic is necrotic lesions on plant leaf or stem in the absence of pathogenic infection, and its exact biological mechanism is varied. By a large-scale screening of our T-DNA mutant population, we identified a mutant rice lesion initiation 1 （rlin1）, which was controlled by a single nuclear recessive gene. Map-based cloning revealed that RLIN1 encoded a putative coproporphyrinogen Ⅲ oxidase in tetrapyrrole biosynthesis pathway. Sequencing results showed that a G to T substitution occurred in the second exon of RLIN1 and led to a missense mutation from Asp to Tyr. Ectopic expression of RLIN1 could rescue rlin1 lesion mimic phenotype. Histochemical analysis demonstrated that lesion formation in rlin1 was light-dependent accompanied by reactive oxygen species accumulated. These results suggest that tetrapyrrole participates in lesion formation in rice.
基金supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA24020301)the National Natural Science Foundation of China(31901524,U20A2025)the Key R&D Program Project of Heilongjiang Province(GA21B014)。
文摘COMPASS or COMPASS-like is a highly conserved polyprotein complex in eukaryotes that is often involved in methylation of histone H3 lysine 4(H3K4).However,the biological function of this complex in rice(Oryza sativa)is unclear.Here,we report the identifiction of their functions in growth and development.The osashl1osashl2 double mutant shows a dwarf and late-flowering phenotype.Lower expression of Ehd1,OsVIL4,and OsMADS51 in the osashl1 osashl2 double mutant background accompanies a delayed vegetative growth phase and photoperiod-sensitive phase compared with that in wild type.Notably,there is less H3K4mono-,di-and tri-methylation genome-wide in the double mutant,in particular less H3K4 tri-methylation at OsVIL4.Consistent with this result,knockout of OsVIL4 gives rise to a late-flowering phenotype similar to that of the osashl1 osashl2 double mutant,suggesting that OsVIL4 is a target of the COMPASS-like complex.In addition,the expression of key genes in brassinosteroid and gibberellic acid metabolism is altered in the osashl1 osashl2 double mutant,suggesting that the COMPASS-like complex regulates plant growth and development by modulating the levels of these two phytohormones.In summary,we demonstrate that OsASHL1 and OsASHL2 are important for floral transition and plant development.
