[Objectives]To investigate the inhibitory effect and possible mechanism of essential oil from Valerianae Jatamansi Rhizoma et Radix on microglia activation induced by lipopolysaccharide(LPS).[Methods]The LPS-induced m...[Objectives]To investigate the inhibitory effect and possible mechanism of essential oil from Valerianae Jatamansi Rhizoma et Radix on microglia activation induced by lipopolysaccharide(LPS).[Methods]The LPS-induced microglia activation model was established and treated with different doses of essential oil from Valerianae Jatamansi Rhizoma et Radix.MTT assay was used to detect cell viability,ELISA to detect IL-6 secretion,RT-PCR to detect mRNA expression levels of IL-6,IL-1β,NF-κB,and IκBα,Western blotting to detect the protein expression of IL-6,IL-1β,NF-κB,IκBα,and their phosphorylated products.[Results]Compared with the normal control group,the model group showed increased IL-6 content(P<0.01),upregulated mRNA and protein levels of IL-6,IL-1β,and NF-κB(P<0.01),and elevated ratio of P-IκBα/IκBα(P<0.05).Compared with the model group,4 and 2μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix reduced the content of IL-6(P<0.05),while 4,2,and 1μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix down-regulated the mRNA and protein levels of IL-6,IL-1β,and NF-κB to varying degrees(P<0.05 or P<0.01),up-regulate the mRNA expression of IκBα(P<0.05 or P<0.01),and decreased the ratio of P-IκBα/IκBα(P<0.05 or P<0.01).[Conclusions]Essential oil from Valerianae Jatamansi Rhizoma et Radix can inhibit LPS-induced microglia activation,and its mechanism may be related to the inhibition of the NF-κB/IκBαsignaling pathway.展开更多
The yeast Saccharomyces cerevisiae is a well-studied unicellular eukaryote with a significant role in the biomanufacturing of natural products,biofuels,and bulk and value-added chemicals,as well as the principal model...The yeast Saccharomyces cerevisiae is a well-studied unicellular eukaryote with a significant role in the biomanufacturing of natural products,biofuels,and bulk and value-added chemicals,as well as the principal model eukaryotic organism utilized for fundamental research.Robust tools for building and optimizing yeast chassis cells were made possible by the quick development of synthetic biology,especially in engineering evolution.In this review,we focused on methods and tools from synthetic biology that are used to design and engineer S.cerevisiae's evolution.A detailed discussion was held regarding transcriptional regulation,template-dependent and template-free approaches.Furthermore,the applications of evolved S.cerevisiae were comprehensively summarized.These included improving environmental stress tolerance and raising cell metabolic performance in the production of biofuels and bulk and value-added chemicals.Finally,the future considerations were briefly discussed.展开更多
基金Supported by Karst Center Project of National Natural Science Foundation of China(U1812403-4-4)High-level Innovative Talents Project in Guizhou Province of Guizhou Provincial Department of Science and Technology[QianKeHeRenCai(2015)4029]+2 种基金Science and Technology Innovation Team for Activity Research of Characteristic Natural Medicine Resources in Guizhou Province[QianKeHeRenCaiTuanDui(2015)4025]Major Project of National Social Science Fund(16ZDA238)Pharmaceutical International Science and Technology Cooperation Base of Guizhou Medical University[QianKeHePingTaiRenCai(2017)5802].
文摘[Objectives]To investigate the inhibitory effect and possible mechanism of essential oil from Valerianae Jatamansi Rhizoma et Radix on microglia activation induced by lipopolysaccharide(LPS).[Methods]The LPS-induced microglia activation model was established and treated with different doses of essential oil from Valerianae Jatamansi Rhizoma et Radix.MTT assay was used to detect cell viability,ELISA to detect IL-6 secretion,RT-PCR to detect mRNA expression levels of IL-6,IL-1β,NF-κB,and IκBα,Western blotting to detect the protein expression of IL-6,IL-1β,NF-κB,IκBα,and their phosphorylated products.[Results]Compared with the normal control group,the model group showed increased IL-6 content(P<0.01),upregulated mRNA and protein levels of IL-6,IL-1β,and NF-κB(P<0.01),and elevated ratio of P-IκBα/IκBα(P<0.05).Compared with the model group,4 and 2μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix reduced the content of IL-6(P<0.05),while 4,2,and 1μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix down-regulated the mRNA and protein levels of IL-6,IL-1β,and NF-κB to varying degrees(P<0.05 or P<0.01),up-regulate the mRNA expression of IκBα(P<0.05 or P<0.01),and decreased the ratio of P-IκBα/IκBα(P<0.05 or P<0.01).[Conclusions]Essential oil from Valerianae Jatamansi Rhizoma et Radix can inhibit LPS-induced microglia activation,and its mechanism may be related to the inhibition of the NF-κB/IκBαsignaling pathway.
基金supported by the National Key Research and Development Program of China(2021YFC2103300)National Natural Science Foundation of China(32161133019 and 32200067)+3 种基金Major Science and Technology Support Program Project in Hebei Province(24292902Z)Key Research and Development Program Projects of Hebei Province(22322905D)Industry University Research Cooperation Project of Shijiazhuang and University of Hebei province(241490062A)supported by Tianjin Industrial Synthetic Biology Innovation Team.
文摘The yeast Saccharomyces cerevisiae is a well-studied unicellular eukaryote with a significant role in the biomanufacturing of natural products,biofuels,and bulk and value-added chemicals,as well as the principal model eukaryotic organism utilized for fundamental research.Robust tools for building and optimizing yeast chassis cells were made possible by the quick development of synthetic biology,especially in engineering evolution.In this review,we focused on methods and tools from synthetic biology that are used to design and engineer S.cerevisiae's evolution.A detailed discussion was held regarding transcriptional regulation,template-dependent and template-free approaches.Furthermore,the applications of evolved S.cerevisiae were comprehensively summarized.These included improving environmental stress tolerance and raising cell metabolic performance in the production of biofuels and bulk and value-added chemicals.Finally,the future considerations were briefly discussed.
