Clinical management of atopic dermatitis(AD)is challenged by its susceptibility to recur-rence,side effects,and high costs.We found that Portulaca oleracea L.-derived nanovesicles(PDNV)exert anti-inflammatory effects ...Clinical management of atopic dermatitis(AD)is challenged by its susceptibility to recur-rence,side effects,and high costs.We found that Portulaca oleracea L.-derived nanovesicles(PDNV)exert anti-inflammatory effects by modulating macrophage M1/M2 polarization.These effects were achieved through pathways including inhibition of nuclear factor-κB(NF-κB)and stimulator of interferon genes(STING)protein expression in diseased tissues,demonstrating their potential to ameliorate AD symptoms.To increase the transdermal permeation of PDNV,dissolvable microneedles composed primar-ily of hyaluronic acid(HA)were developed as an adjunctive means of delivery.Meanwhile,polysaccha-rides of Portulaca oleracea L.,which were synergistic with PDNV,were used as microneedle constituent materials to enhance the mechanical properties and physical stability of HA.This new means of delivery significantly improves the treatment of AD and also provides new options for the efficient utilization of plant extracellular vesicles and the treatment of AD.In addition,transcriptomic analysis of PDNV showed that the mRNAs of Portulaca oleracea L.are closest to those of ferns,which may shed light on related evolutionary and plant species identification studies.展开更多
基金supported by the National Natural Science Foundation of China(No.82474197)the Program for Professor of Special Appointment(Eastern Scholar)at Shanghai Institutions of Higher Learning(No.TP2020054,China)+1 种基金Shanghai Seventh People’s Hospital Medical Institution Preparation and Innovative Chinese Medicine R&D(Open Bidding for Selecting the Best Candidates)Project(No.QYCXZY250204,China)the Program for Shanghai High-Level Local University Innovation Team(No.SZY20220315,China).
文摘Clinical management of atopic dermatitis(AD)is challenged by its susceptibility to recur-rence,side effects,and high costs.We found that Portulaca oleracea L.-derived nanovesicles(PDNV)exert anti-inflammatory effects by modulating macrophage M1/M2 polarization.These effects were achieved through pathways including inhibition of nuclear factor-κB(NF-κB)and stimulator of interferon genes(STING)protein expression in diseased tissues,demonstrating their potential to ameliorate AD symptoms.To increase the transdermal permeation of PDNV,dissolvable microneedles composed primar-ily of hyaluronic acid(HA)were developed as an adjunctive means of delivery.Meanwhile,polysaccha-rides of Portulaca oleracea L.,which were synergistic with PDNV,were used as microneedle constituent materials to enhance the mechanical properties and physical stability of HA.This new means of delivery significantly improves the treatment of AD and also provides new options for the efficient utilization of plant extracellular vesicles and the treatment of AD.In addition,transcriptomic analysis of PDNV showed that the mRNAs of Portulaca oleracea L.are closest to those of ferns,which may shed light on related evolutionary and plant species identification studies.
