Objectives:Walnut protein-galactooligosaccharide(WalPI-GOS)nanoparticles were used to prepare high internal phase Pickering emulsions(HIPPEs).Materials and Methods:The entrapment properties of HIPPEs for cinnamon oil ...Objectives:Walnut protein-galactooligosaccharide(WalPI-GOS)nanoparticles were used to prepare high internal phase Pickering emulsions(HIPPEs).Materials and Methods:The entrapment properties of HIPPEs for cinnamon oil were investigated by varying the volume ratios of camellia and cinnamon oils(cinnamon oil contents:0%,2.5%,5.0%,10%,15%,and 20%),and the droplet size,rheological properties,Raman spectroscopy results,microstructure,thermal stability,storage stability,and antioxidant activity of HIPPEs were determined.Results:The droplet size of HIPPEs increased with increasing cinnamon oil content.Among the samples,HIPPEs enriched with the cinnamon oil content of 10%had the highest storage modulus,loss modulus,and apparent viscosity(13.64 Pa·s).However,the thixotropic recovery ability of HIPPEs decreased with the increase in cinnamon oil content.Raman spectroscopy and microstructural analysis revealed that proteins covalently cross-linked with cinnamaldehyde to form a three-dimensional network structure,which showed the highest stability when the cinnamon oil content was 10%.HIPPEs exhibited high thermal stability without delamination after heating,as well as good storage stability without delamination or discoloration after 15 d of storage at 25℃ and 50℃.Among the samples,HIPPEs enriched with 10%cinnamon oil had the lowest peroxide and malondialdehyde values during storage.The addition of cinnamon oil significantly enhanced the antioxidant activity of HIPPEs.Conclusions:The best overall performance of HIPPEs was achieved at a cinnamon oil content of 10%.This result provides a theoretical foundation for the development of WalPI and the application of cinnamon oil in food,as well as a theoretical basis for the development of novel food delivery systems.展开更多
Objectives To enhance the stability of proliposomes co-encapsulating Rosa roxburghii Tratt seed oil(RSO)andβ-carotene(βC),sodium alginate(SA)and chitosan(CS)were utilized for bilayer surface modification.In this stu...Objectives To enhance the stability of proliposomes co-encapsulating Rosa roxburghii Tratt seed oil(RSO)andβ-carotene(βC),sodium alginate(SA)and chitosan(CS)were utilized for bilayer surface modification.In this study,the proliposomes containing RSO andβC are referred to as Ps,while the modified formulations are denoted as sodium alginate-chitosanmodified proliposomes(SA-CS-Ps).Materials and methods The physicochemical properties,structural characteristics,thermal stability,microstructure,rheological behavior,storage stability,and oxidation kinetics of SA-CS-Ps were investigated and compared with those of unmodified Ps and CS monolayer-modified Ps(CS-Ps).Results The particle size and turbidity of Ps increased with the addition of modification layers,reaching 563.73 nm and0.697,respectively,for SA-CS-Ps.CS and SA formed a protective bilayer through electrostatic interactions,which preserved the spherical structure of Ps while enhancing its apparent viscosity and stress.Thermal stability analysis indicated that the phase transition temperatures of CS-Ps and SA-CS-Ps were elevated by 10.57 and 15.76℃,respectively,compared with that of unmodified Ps.During storage,the retention rates(RRs)of RSO andβC followed the order SA-CS-Ps>CS-Ps>Ps,whereas malondialdehyde and peroxide values exhibited the opposite trend.Elevated temperatures accelerated oxidation,thereby compromising the protective effect of the carriers.After 24 d at 4℃,SA-CSPs demonstrated superior stability,with malondialdehyde of 0.016μg/mg,peroxide value of 2.697 mmol/kg,and RRs of RSO of 97.15%andβC of 96.37%.The oxidation kinetics conformed to a first-order reaction model.Conclusions Given its enhanced protection against degradation and stability,SA-CS-Ps represents a promising candidate for the delivery of sensitive bioactive compounds.This study provides a theoretical basis for the application of bilayer-modified proliposomes in functional food delivery systems requiring enhanced stability.展开更多
基金supported by the Yunnan Fundamental Research Projects(No.202401AS070012)the Youth Project of‘Xingdian Talent Support Plan’in Yunnan Province(YNWR-QNBJ-2018-046).
文摘Objectives:Walnut protein-galactooligosaccharide(WalPI-GOS)nanoparticles were used to prepare high internal phase Pickering emulsions(HIPPEs).Materials and Methods:The entrapment properties of HIPPEs for cinnamon oil were investigated by varying the volume ratios of camellia and cinnamon oils(cinnamon oil contents:0%,2.5%,5.0%,10%,15%,and 20%),and the droplet size,rheological properties,Raman spectroscopy results,microstructure,thermal stability,storage stability,and antioxidant activity of HIPPEs were determined.Results:The droplet size of HIPPEs increased with increasing cinnamon oil content.Among the samples,HIPPEs enriched with the cinnamon oil content of 10%had the highest storage modulus,loss modulus,and apparent viscosity(13.64 Pa·s).However,the thixotropic recovery ability of HIPPEs decreased with the increase in cinnamon oil content.Raman spectroscopy and microstructural analysis revealed that proteins covalently cross-linked with cinnamaldehyde to form a three-dimensional network structure,which showed the highest stability when the cinnamon oil content was 10%.HIPPEs exhibited high thermal stability without delamination after heating,as well as good storage stability without delamination or discoloration after 15 d of storage at 25℃ and 50℃.Among the samples,HIPPEs enriched with 10%cinnamon oil had the lowest peroxide and malondialdehyde values during storage.The addition of cinnamon oil significantly enhanced the antioxidant activity of HIPPEs.Conclusions:The best overall performance of HIPPEs was achieved at a cinnamon oil content of 10%.This result provides a theoretical foundation for the development of WalPI and the application of cinnamon oil in food,as well as a theoretical basis for the development of novel food delivery systems.
基金supported by the Basic Research Projects of Free Exploration Category of Central Guided Local Science and Technology Development Funds(No.202407AA110007)the Yunnan Provincial Talent Reserve Project for Young and Middle-aged Academic and Technical Leaders(No.202405AC350032)+1 种基金the Ten Thousand People Plan of Yunnan Province,Young Top-notch Personnel(No.YNWR-QNBJ-2018-046)the Yunnan Fundamental Research Projects(No.202401AS070012),China。
文摘Objectives To enhance the stability of proliposomes co-encapsulating Rosa roxburghii Tratt seed oil(RSO)andβ-carotene(βC),sodium alginate(SA)and chitosan(CS)were utilized for bilayer surface modification.In this study,the proliposomes containing RSO andβC are referred to as Ps,while the modified formulations are denoted as sodium alginate-chitosanmodified proliposomes(SA-CS-Ps).Materials and methods The physicochemical properties,structural characteristics,thermal stability,microstructure,rheological behavior,storage stability,and oxidation kinetics of SA-CS-Ps were investigated and compared with those of unmodified Ps and CS monolayer-modified Ps(CS-Ps).Results The particle size and turbidity of Ps increased with the addition of modification layers,reaching 563.73 nm and0.697,respectively,for SA-CS-Ps.CS and SA formed a protective bilayer through electrostatic interactions,which preserved the spherical structure of Ps while enhancing its apparent viscosity and stress.Thermal stability analysis indicated that the phase transition temperatures of CS-Ps and SA-CS-Ps were elevated by 10.57 and 15.76℃,respectively,compared with that of unmodified Ps.During storage,the retention rates(RRs)of RSO andβC followed the order SA-CS-Ps>CS-Ps>Ps,whereas malondialdehyde and peroxide values exhibited the opposite trend.Elevated temperatures accelerated oxidation,thereby compromising the protective effect of the carriers.After 24 d at 4℃,SA-CSPs demonstrated superior stability,with malondialdehyde of 0.016μg/mg,peroxide value of 2.697 mmol/kg,and RRs of RSO of 97.15%andβC of 96.37%.The oxidation kinetics conformed to a first-order reaction model.Conclusions Given its enhanced protection against degradation and stability,SA-CS-Ps represents a promising candidate for the delivery of sensitive bioactive compounds.This study provides a theoretical basis for the application of bilayer-modified proliposomes in functional food delivery systems requiring enhanced stability.
