Rapid screening of inorganic arsenic(iAs)in groundwater used for drinking by hundreds of millions of mostly rural residents worldwide is crucial for health protection.Most commercial field test kits are based on the G...Rapid screening of inorganic arsenic(iAs)in groundwater used for drinking by hundreds of millions of mostly rural residents worldwide is crucial for health protection.Most commercial field test kits are based on the Gutzeit reaction that uses mercury-based reagents for color development,an environmental concern that increasingly limits its utilization.This study further improves the Molybdenum Blue(MB)colorimetric method to allow for faster screening with more stable reagents.More importantly,a portable three-channel colorimeter is developed for screening iAs relative to the WHO drinking water guideline value(10μg/L).Adding the reducing reagents in sequence not only prolongs the storage time to>7 days,but also accelerates the color development time to 6 min in conjunction with lowering the H_(2)SO_(4) concentration in chromogenic reagents.The optimal pH ranges from 1.2 to 1.3 and is achieved by acidifying groundwater to 1%(V/V)HCl.With detection limits of 3.7μg/L for inorganic arsenate(iAs(V))and 3.8μg/L for inorganic arsenite(iAs(Ⅲ)),testing groundwater with-10μg/L of As has a precision<20%.The method works well for a range of phosphate concentrations of 48-950μg/L(0.5-10μmol/L).Concentrations of total_iAs(6-300μg/L),iAs(V)(6-230μg/L)and iAs(Ⅲ)(0-170μg/L)for 14 groundwater samples from Yinchuan Plain,Pearl River Delta,and Jianghan Plain,are in excellent agreements(linear regression slope:0.969-1.029)with the benchmark methods.The improved chemistry here lays the foundation for the MB colorimetric method to become a commercially viable screening tool,with further engineering and design improvement of the colorimeter.展开更多
Zebrafish larvae are useful for identifying chemicals against lateral line(LL)hair cell(HC)damage and this type of chemical screen mainly focuses on searching for protectors against cell death.To expand the candidate ...Zebrafish larvae are useful for identifying chemicals against lateral line(LL)hair cell(HC)damage and this type of chemical screen mainly focuses on searching for protectors against cell death.To expand the candidate pool of HC protectors,a self-built acoustic escape response(AER)-detecting system was developed to apply both low-frequency near-field sound transmission and AER image acquisition/processing modules.The device quickly confirmed the changed LL HC functions caused by most known ototoxins,protectors,and neural transmission modifiers,or knockdown of LL HC-expressing genes.With ten devices wired in tandem,five‘hit’chemicals were identified from 124 cyclin-dependent kinase inhibitors to partially restore cisplatin-damaged AER in less than a day.AS2863619,ribociclib,and SU9516 among the hits,protected the HCs in the mouse cochlea.Therefore,using free-swimming larval zebrafish,the self-made AER-detecting device can efficiently identify compounds that are protective against HC damage,including cell death and loss-of-function.展开更多
Drosophila dEAAT2, a member of the excitatory amino-acid transporter(EAAT) family, has been described as mediating the high-affinity transport of taurine, which is a free amino-acid abundant in both insects and mammal...Drosophila dEAAT2, a member of the excitatory amino-acid transporter(EAAT) family, has been described as mediating the high-affinity transport of taurine, which is a free amino-acid abundant in both insects and mammals. However, the role of taurine and its transporter in hearing is not clear. Here, we report that dEAAT2 is required for the larval startle response to sound stimuli. d EAAT2 was found to be enriched in the distal region of chordotonal neurons where sound transduction occurs. The Ca2+imaging and electrophysiological results showed that disrupted dEAAT2 expression significantly reduced the response of chordotonal neurons to sound.More importantly, expressing d EAAT2 in the chordotonal neurons rescued these mutant phenotypes. Taken together,these findings indicate a critical role for Drosophila dEAAT2 in sound transduction by chordotonal neurons.展开更多
With the modernization of industrial production,various automatic following technologies have gradually penetrated into people's lives.Among them,the application fields that follow mobile balancing vehicles range ...With the modernization of industrial production,various automatic following technologies have gradually penetrated into people's lives.Among them,the application fields that follow mobile balancing vehicles range from the structured environment of factories to the daily living environment.The purpose of this subject is to be able to use UWB positioning technology to realize that the balanced trolley is an automatic following function,which mainly uses the ranging function in UWB positioning technology,uses the TOF ranging principle and embedded basic knowledge,and changes the positioning algorithm to a following algorithm.The distance between the target object and the person is effectively determined by two tags set on the trolley and a base station in the hand to determine the distance,and the distance information is transmitted through the serial port.By writing an algorithm,the distance information is converted to the driving information of the trolley,and the driving information is sent to the trolley to control the trolley,thereby realizing the task of balancing the trolley to follow the specified target in a simple indoor environment.展开更多
Polarization underwater imaging is of great potential to target detection in turbid water. Typical methods are challenged by the requirement on degrees of polarization(Do Ps) of both target light and backscattering. A...Polarization underwater imaging is of great potential to target detection in turbid water. Typical methods are challenged by the requirement on degrees of polarization(Do Ps) of both target light and backscattering. A polarization descattering imaging method was developed using the Mueller matrix, which in turn derived a depolarization(Dep) index from the Mueller matrix to characterize scattering media by estimating the transmittance map by combining a developed optimal function.By quantifying light attenuation with the transmittance map, a clear vision of targets can be recovered. Only using the information of scattering media, the underwater vision under diverse water turbidity was enhanced by the results of experimental data.展开更多
In the process of coping with energy and environmental protection issues,technologies such as energy materials,energy devices,and energy systems have made great progress.With excellent performance,film capacitors play...In the process of coping with energy and environmental protection issues,technologies such as energy materials,energy devices,and energy systems have made great progress.With excellent performance,film capacitors play an increasingly important role in energyrelated fields.With the increase of application scenarios and the continuous development of film material technology,it is urgent to establish a better theoretical connection from films to capacitors.First,the main components of the capacitor including the film and the positional relationships among them are given.Then,from the two perspectives of indirect calculation according to the volume and the direct calculation according to the winding process,the equation between the dielectric constant of films and the corresponding capacitance of capacitors is established.Further,the measurement data and error analysis results of the built test platform prove the accuracy and great potential of the proposed calculation methods.In addition,error sources,including film thickness uniformity,are listed.Finally,the challenges faced by the proposed calculation methods and the paths that can be referenced for future research are summarised and discussed.展开更多
Zebrafish an emerging animal model for studies comparability with humans, good accessibility to the hearing utilize this animal model, methodologies need to be used to on auditory system. This model presents high org...Zebrafish an emerging animal model for studies comparability with humans, good accessibility to the hearing utilize this animal model, methodologies need to be used to on auditory system. This model presents high organ, and high throughput capacity. To better quantify the hearing function of the zebrafish. Zebrafish displays a series of innate and robust behavior related to its auditory function. Here, we reviewed the advantage of using zebrafish in auditory research and then introduced three behavioral tests, as follows： the startle response, the vestibular-ocular reflex, and rheotaxis. These tests are discussed in terms of their physiological characteristics, up-to-date technical development, and apparatus description. Test limitation and areas to improve are also introduced. Finally, we revealed the feasibility of these applications in zebrafish behavioral assessment and their potential in the high-throughput screening on hearing-related genes and drugs.展开更多
基金the National Key R&D Program of China(No.2021YFA0715900)the National Natural Science Foundation of China(No.41831279)+2 种基金the Shenzhen Key Laboratory of Precision Measurement and Early Warning Technology for Urban Environmental Health Risks(No.ZDSYS20220606100604008)the Guangdong Province Bureau of Education(No.2020KCXTD006)the Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control(No.2023B1212060002).
文摘Rapid screening of inorganic arsenic(iAs)in groundwater used for drinking by hundreds of millions of mostly rural residents worldwide is crucial for health protection.Most commercial field test kits are based on the Gutzeit reaction that uses mercury-based reagents for color development,an environmental concern that increasingly limits its utilization.This study further improves the Molybdenum Blue(MB)colorimetric method to allow for faster screening with more stable reagents.More importantly,a portable three-channel colorimeter is developed for screening iAs relative to the WHO drinking water guideline value(10μg/L).Adding the reducing reagents in sequence not only prolongs the storage time to>7 days,but also accelerates the color development time to 6 min in conjunction with lowering the H_(2)SO_(4) concentration in chromogenic reagents.The optimal pH ranges from 1.2 to 1.3 and is achieved by acidifying groundwater to 1%(V/V)HCl.With detection limits of 3.7μg/L for inorganic arsenate(iAs(V))and 3.8μg/L for inorganic arsenite(iAs(Ⅲ)),testing groundwater with-10μg/L of As has a precision<20%.The method works well for a range of phosphate concentrations of 48-950μg/L(0.5-10μmol/L).Concentrations of total_iAs(6-300μg/L),iAs(V)(6-230μg/L)and iAs(Ⅲ)(0-170μg/L)for 14 groundwater samples from Yinchuan Plain,Pearl River Delta,and Jianghan Plain,are in excellent agreements(linear regression slope:0.969-1.029)with the benchmark methods.The improved chemistry here lays the foundation for the MB colorimetric method to become a commercially viable screening tool,with further engineering and design improvement of the colorimeter.
基金supported by grants from Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions(2023SHIBS0002)the National Key Research and Development Program of China(2023YFF1203505)+5 种基金the National Key R&D Program of China(2024YFC2417900)Shenzhen Municipal Science and Technology Innovation Council(202110293000007)Shenzhen Science and Technology Program(KJZD20230923114909019)the National Key R&D Program of China(2018YFA0801006)the National Natural Science Foundation of China(31771618)Guangdong Natural Science Foundation(2022A1515011878).
文摘Zebrafish larvae are useful for identifying chemicals against lateral line(LL)hair cell(HC)damage and this type of chemical screen mainly focuses on searching for protectors against cell death.To expand the candidate pool of HC protectors,a self-built acoustic escape response(AER)-detecting system was developed to apply both low-frequency near-field sound transmission and AER image acquisition/processing modules.The device quickly confirmed the changed LL HC functions caused by most known ototoxins,protectors,and neural transmission modifiers,or knockdown of LL HC-expressing genes.With ten devices wired in tandem,five‘hit’chemicals were identified from 124 cyclin-dependent kinase inhibitors to partially restore cisplatin-damaged AER in less than a day.AS2863619,ribociclib,and SU9516 among the hits,protected the HCs in the mouse cochlea.Therefore,using free-swimming larval zebrafish,the self-made AER-detecting device can efficiently identify compounds that are protective against HC damage,including cell death and loss-of-function.
基金supported by funds from The Ministry of Science and Technology of China (2017YFA0103900 and 2016YFA0502800)The National Natural Science Foundation of China (31571083)+6 种基金supported by The National Natural Science Foundation of China (81470701)The National Natural Science Foundation of China (81771882)The Program for Professor of Special Appointment (Eastern Scholar of Shanghai TP2014008)The Shanghai Rising-Star Program (14QA1400800)a grant from the Young 1000 Talent Program of China to ZYThe Fundamental Research (Discipline Layout) Foundation from Shenzhen Committee of Science, Technology and Innovation (JCYJ20170817111912585) to FC
文摘Drosophila dEAAT2, a member of the excitatory amino-acid transporter(EAAT) family, has been described as mediating the high-affinity transport of taurine, which is a free amino-acid abundant in both insects and mammals. However, the role of taurine and its transporter in hearing is not clear. Here, we report that dEAAT2 is required for the larval startle response to sound stimuli. d EAAT2 was found to be enriched in the distal region of chordotonal neurons where sound transduction occurs. The Ca2+imaging and electrophysiological results showed that disrupted dEAAT2 expression significantly reduced the response of chordotonal neurons to sound.More importantly, expressing d EAAT2 in the chordotonal neurons rescued these mutant phenotypes. Taken together,these findings indicate a critical role for Drosophila dEAAT2 in sound transduction by chordotonal neurons.
文摘With the modernization of industrial production,various automatic following technologies have gradually penetrated into people's lives.Among them,the application fields that follow mobile balancing vehicles range from the structured environment of factories to the daily living environment.The purpose of this subject is to be able to use UWB positioning technology to realize that the balanced trolley is an automatic following function,which mainly uses the ranging function in UWB positioning technology,uses the TOF ranging principle and embedded basic knowledge,and changes the positioning algorithm to a following algorithm.The distance between the target object and the person is effectively determined by two tags set on the trolley and a base station in the hand to determine the distance,and the distance information is transmitted through the serial port.By writing an algorithm,the distance information is converted to the driving information of the trolley,and the driving information is sent to the trolley to control the trolley,thereby realizing the task of balancing the trolley to follow the specified target in a simple indoor environment.
基金This work was supported by the National Natural Science Foundation of China(NSFC)(Nos.62075175 and 62005203)the Key Laboratory of Optical Engineering,Chinese Academy of Sciences。
文摘Polarization underwater imaging is of great potential to target detection in turbid water. Typical methods are challenged by the requirement on degrees of polarization(Do Ps) of both target light and backscattering. A polarization descattering imaging method was developed using the Mueller matrix, which in turn derived a depolarization(Dep) index from the Mueller matrix to characterize scattering media by estimating the transmittance map by combining a developed optimal function.By quantifying light attenuation with the transmittance map, a clear vision of targets can be recovered. Only using the information of scattering media, the underwater vision under diverse water turbidity was enhanced by the results of experimental data.
基金National Key Research and Development Program of China,Grant/Award Number:2021YFB2401504National Natural Science Foundation of China,Grant/Award Number:51937007。
文摘In the process of coping with energy and environmental protection issues,technologies such as energy materials,energy devices,and energy systems have made great progress.With excellent performance,film capacitors play an increasingly important role in energyrelated fields.With the increase of application scenarios and the continuous development of film material technology,it is urgent to establish a better theoretical connection from films to capacitors.First,the main components of the capacitor including the film and the positional relationships among them are given.Then,from the two perspectives of indirect calculation according to the volume and the direct calculation according to the winding process,the equation between the dielectric constant of films and the corresponding capacitance of capacitors is established.Further,the measurement data and error analysis results of the built test platform prove the accuracy and great potential of the proposed calculation methods.In addition,error sources,including film thickness uniformity,are listed.Finally,the challenges faced by the proposed calculation methods and the paths that can be referenced for future research are summarised and discussed.
文摘Zebrafish an emerging animal model for studies comparability with humans, good accessibility to the hearing utilize this animal model, methodologies need to be used to on auditory system. This model presents high organ, and high throughput capacity. To better quantify the hearing function of the zebrafish. Zebrafish displays a series of innate and robust behavior related to its auditory function. Here, we reviewed the advantage of using zebrafish in auditory research and then introduced three behavioral tests, as follows： the startle response, the vestibular-ocular reflex, and rheotaxis. These tests are discussed in terms of their physiological characteristics, up-to-date technical development, and apparatus description. Test limitation and areas to improve are also introduced. Finally, we revealed the feasibility of these applications in zebrafish behavioral assessment and their potential in the high-throughput screening on hearing-related genes and drugs.
