The archeospores produced from the blades of Pyropia yezoensis could develop into new blades,which is of significant both in cultivation and research.Nevertheless,the molecular mechanisms behind the formation and rele...The archeospores produced from the blades of Pyropia yezoensis could develop into new blades,which is of significant both in cultivation and research.Nevertheless,the molecular mechanisms behind the formation and release of archeospores remained unclear.In this study,two strains of P.yezoensis with similar genetic backgrounds and opposite abilities for archeospores formation were used for genomic and transcriptomic analysis.Based on whole-genome resequencing,a total of 54,439 SNPs and 12,922 InDels were detected.Specifically,211 SNPs and 8 InDels in coding regions could introduce codon change or frameshift mutation,resulted in sequence variations of corresponding encoded proteins.Furthermore,a total of 2888 differentially expressed genes(DEGs)between two strains were identified based on transcriptomic analysis,and 68 DEGs shared SNPs or InDels according to resequencing analysis,which may be associated with the processes related to the formation of archeospores.This study integrates genomic approaches to identify candidate genes and loci related to archeospores formation in P.yezoensis,laying a foundation for elucidating the regulatory mechanisms of archeospores formation and release.展开更多
基金supported by the National Key R&D Program of China(2023YFD2400102,2023YFD2400105)the Open Program of Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province(2022fjscq01).
文摘The archeospores produced from the blades of Pyropia yezoensis could develop into new blades,which is of significant both in cultivation and research.Nevertheless,the molecular mechanisms behind the formation and release of archeospores remained unclear.In this study,two strains of P.yezoensis with similar genetic backgrounds and opposite abilities for archeospores formation were used for genomic and transcriptomic analysis.Based on whole-genome resequencing,a total of 54,439 SNPs and 12,922 InDels were detected.Specifically,211 SNPs and 8 InDels in coding regions could introduce codon change or frameshift mutation,resulted in sequence variations of corresponding encoded proteins.Furthermore,a total of 2888 differentially expressed genes(DEGs)between two strains were identified based on transcriptomic analysis,and 68 DEGs shared SNPs or InDels according to resequencing analysis,which may be associated with the processes related to the formation of archeospores.This study integrates genomic approaches to identify candidate genes and loci related to archeospores formation in P.yezoensis,laying a foundation for elucidating the regulatory mechanisms of archeospores formation and release.
