Objective:To evaluate the quality of Lygodium japonicum(Thunb.)Sw.(L.japonicum,Hai Jin Sha)by comparing its components without stewed(W)and stewed(S)using ultra-high-performance liquid chromatography(UHPLC)and chemome...Objective:To evaluate the quality of Lygodium japonicum(Thunb.)Sw.(L.japonicum,Hai Jin Sha)by comparing its components without stewed(W)and stewed(S)using ultra-high-performance liquid chromatography(UHPLC)and chemometric analysis.Additionally,network pharmacology was employed to investigate the possible mechanisms of action of L.japonicum in the urinary calculi(UC)treatment.Methods:A fingerprinting method was established to identify components through UHPLC-tandem mass spectrometry.Chemometric techniques were used to compare the L.japonicum extraction methods.Furthermore,various network pharmacological approaches were used to identify and analyze the potential targets of the identified components in relation to UC.Results:The W and S extracts were distributed into two distinct clusters.Significant differences in the levels of protocatechuic aldehyde,caffeic acid,and p-coumaric acid were observed between S and W.Network pharmacology analysis revealed that the primary targets of L.japonicum in the UC treatment were serum albumin and epidermal growth factor receptors,with potential active components including protocatechuic acid and caffeic acid.Conclusion:This study comprehensively examined the therapeutic components of L.japonicum before and after boiling,shedding light on its potential mechanisms of action in UC treatment.These findings offer valuable insights into the development and utilization of L.japonicum resources.展开更多
Objective:The current investigation aimed to determine the appropriate dosage form by comparing solid dispersion and liposome to achieve the purpose of improving the solubility and bioavailability of linarin.Methods:L...Objective:The current investigation aimed to determine the appropriate dosage form by comparing solid dispersion and liposome to achieve the purpose of improving the solubility and bioavailability of linarin.Methods:Linarin solid dispersion(LSD)and linarin liposome(LL)were developed via the solvent method and the thin film hydration method respectively.The Transwell chamber model of Caco-2 cells was established to evaluate the absorption of drug.The pharmacokinetics of linarin,LSD and LL in rats after ig administration were carried out by high performance liquid chromatography(HPLC)method.Results:The solubility of LSD and LL was severally 3.29 times and 3.09 times than that of linarin.The permeation coefficients of LSD and LL were greater than 10^(-6),indicating that the absorption of LSD and LL were both better than linarin.The bioavailability of the LSD was 3.363 times higher than that of linarin,and the bioavailability of LL was 0.9886 times higher than that of linarin.Conclusion:The linarin was more suitable for making solid dispersion to enhance its solubility and bioavailability.展开更多
基金supported by Ministry of Industry and Information Technology of the People's Republic of China 2022 Industrial Technology Basic Public Service Platform Project-Traditional Chinese Medicine Whole Industry Chain Quality and Technology Service Platform(2022-230-221)Foshan Nanhai District Key Area Science and Technology Research Project[Nanke(2023)20-18].
文摘Objective:To evaluate the quality of Lygodium japonicum(Thunb.)Sw.(L.japonicum,Hai Jin Sha)by comparing its components without stewed(W)and stewed(S)using ultra-high-performance liquid chromatography(UHPLC)and chemometric analysis.Additionally,network pharmacology was employed to investigate the possible mechanisms of action of L.japonicum in the urinary calculi(UC)treatment.Methods:A fingerprinting method was established to identify components through UHPLC-tandem mass spectrometry.Chemometric techniques were used to compare the L.japonicum extraction methods.Furthermore,various network pharmacological approaches were used to identify and analyze the potential targets of the identified components in relation to UC.Results:The W and S extracts were distributed into two distinct clusters.Significant differences in the levels of protocatechuic aldehyde,caffeic acid,and p-coumaric acid were observed between S and W.Network pharmacology analysis revealed that the primary targets of L.japonicum in the UC treatment were serum albumin and epidermal growth factor receptors,with potential active components including protocatechuic acid and caffeic acid.Conclusion:This study comprehensively examined the therapeutic components of L.japonicum before and after boiling,shedding light on its potential mechanisms of action in UC treatment.These findings offer valuable insights into the development and utilization of L.japonicum resources.
基金financed by Natural Science Foundation of Henan Province(Grant No.182300410348)。
文摘Objective:The current investigation aimed to determine the appropriate dosage form by comparing solid dispersion and liposome to achieve the purpose of improving the solubility and bioavailability of linarin.Methods:Linarin solid dispersion(LSD)and linarin liposome(LL)were developed via the solvent method and the thin film hydration method respectively.The Transwell chamber model of Caco-2 cells was established to evaluate the absorption of drug.The pharmacokinetics of linarin,LSD and LL in rats after ig administration were carried out by high performance liquid chromatography(HPLC)method.Results:The solubility of LSD and LL was severally 3.29 times and 3.09 times than that of linarin.The permeation coefficients of LSD and LL were greater than 10^(-6),indicating that the absorption of LSD and LL were both better than linarin.The bioavailability of the LSD was 3.363 times higher than that of linarin,and the bioavailability of LL was 0.9886 times higher than that of linarin.Conclusion:The linarin was more suitable for making solid dispersion to enhance its solubility and bioavailability.
