Objectives:This systematic review and meta-analysis aimed to identify and synthesize the factors correlated with posttraumatic growth(PTG)in patients with colorectal cancer(CRC).Methods:PubMed,Web of Science,Embase,Ps...Objectives:This systematic review and meta-analysis aimed to identify and synthesize the factors correlated with posttraumatic growth(PTG)in patients with colorectal cancer(CRC).Methods:PubMed,Web of Science,Embase,PsycINFO,CINAHL,Cochrane Library,China National Knowledge Infrastructure(CNKI),Wanfang database,China Science and Technology Journal Database(VIP)and SinoMed were searched for studies that reported data on the correlated factors associated with PTG in patients with CRC from inception to September 3,2024.The methodological quality of the included studies was assessed via the Agency for Healthcare Research and Quality(AHRQ)methodology checklist and the Newcastle-Ottawa Scale(NOS).Pearson correlation coefficient(r)was utilized to indicate effect size.Meta-analysis was conducted in R Studio.Results:Thirty-one eligible studies encompassing 6,400 participants were included in this review.Correlated factors were identified to be significantly associated with PTG in patients with CRC including demographic factors:residential area(r=0.13),marital status(r=0.10),employment status(r=0.18),education level(r=0.19),income level(r=0.16);disease-related factors:time since surgery(r=0.17),stoma-related complications(r=0.14),health-promoting behavior(r=0.46),and sexual function(r=0.17);psychosocial factors:confrontation coping(r=0.68),avoidance coping(r=-0.65),deliberate rumination(r=0.56),social support(r=0.47),family function(r=0.50),resilience(r=0.53),selfefficacy(r=0.91),self-compassion(r=-0.32),psychosocial adjustment(r=0.39),gratitude(r=0.45),stigma(r=-0.65),self-perceived burden(r=-0.31),fear of cancer recurrence(r=-0.45);and quality of life(r=0.32).Conclusions:This meta-analysis identified 23 factors associated with PTG in CRC patients.Medical workers can combine those relevant factors from the perspective of positive psychology,further explore the occurrence and development mechanism of PTG,and establish targeted interventions to promote PTG.展开更多
Microplastics(MPs)have garnered significant international scrutiny as an emerging environmental pollutant,constituting one of the four principal global environmental threats and posing potential health hazards to huma...Microplastics(MPs)have garnered significant international scrutiny as an emerging environmental pollutant,constituting one of the four principal global environmental threats and posing potential health hazards to humans.However,data on the impact of MPs on the early life of the commercially important fish remain limited.In this study,polystyrene microspheres(PS-MPs)(1 and 5μm)were used to investigate the effects of MPs on the growth,development,and metabolism in early life stages of large yellow croaker Pseudosciaena crocea.Results indicate that MPs were enriched in the gastrointestinal tract and gills of the fish.In addition,PS-MPs(1μm)exhibited no obvious effects on embryo hatching and heart rates,while increased the mortality rate(23.00%vs.control 14.99%)and decreased the body length(4098.61±447.03μm vs.control with 2827.04±254.75μm)of the larvae at the highest exposure concentration(5×10^(4)items/L).Metabolomics analysis revealed that PS-MPs(5μm)induced mild perturbations in phospholipid metabolism,specifically alterations in phosphatidylethanolamine(PE)levels.These changes influenced the cell membranes of juvenile fish,and consequently elicited inflammatory responses,disrupted lipid homeostasis,and affected other critical physiological processes.Ultimately,these effects may avoid the growth retardation and potential mortality.Therefore,PS-MPs could affect negatively the fish health in the early life stage,which has implications for aquatic ecosystems.展开更多
Objective： The purposes of this study were to assess the efficacy of allogeneic hematopoietic stem cell transplantation （HSCT） for acute leukemia （AL） and analyze the factors affecting the prognosis of these pati...Objective： The purposes of this study were to assess the efficacy of allogeneic hematopoietic stem cell transplantation （HSCT） for acute leukemia （AL） and analyze the factors affecting the prognosis of these patients. Methods： The clinical and follow-up data of 93 AL patients （median age, 30 years） undergoing allogeneic HSCT in Xiangya Hospital over the past 12 years were collected, and the potential factors affecting the efficacy and prognosis of allogeneic HSCT patients were determined. Results： Hematopoietic reconstitution was achieved in 90 patients. At the last follow-up, the incidences of severe acute graft versus host disease （aGvHD） and extensive chronic GvHD （cGvHD） were 14.0% and 20.0%, the 3-year cumulative incidence of transplantation related mortality （TRM） and relapse rate were 16.8%±6.1% and 21.3%±6.7%, and the estimated 3-year overall survival （OS） and disease-free survival （DFS） of the patients were 64.6%±5.4% and 56.5%±5.5%, respectively. Univariate analysis indicated that age older than 40 years, HLA mismatch, and severe lung infection within the first 100 days after transplantation were risk factors for severe aGvHD, age older than 40 years, HLA mismatch, severe lung infection within the first 100 days after transplantation, and severe aGvHD were risk factors for TRM, high-risk AL and lack of cGvHD were risk factors for relapse （all P〈0.05）. Survival estimation showed that HLA mismatch, severe lung infection occurring within the first 100 days post-transplantation, high-risk AL severe aGvHD and lack of cGvHD were risk factors associated with poor prognosis （all P〈0.05）. Further multivariate analyses revealed that severe lung infection within the first 100 days post-transplantation, severe aGvHD and lack of cGvHD were independent risk factors for unfavorable outcomes （all P〈0.05）. Conclusions： Allogeneic HSCT can improve the DFS of AL patients, and severe lung infection within the first 100 days post-transplantation, severe aGvHD and lack of cGvHD are independent risk factors affecting the prognosis.展开更多
The guideline of the Infectious Diseases Society of America (IDSA) about candidiasis is a key standard for clinical doctors to treat patients, and textbook to teach medical students. However, in the progress of clinic...The guideline of the Infectious Diseases Society of America (IDSA) about candidiasis is a key standard for clinical doctors to treat patients, and textbook to teach medical students. However, in the progress of clinical treatment and documents checking, the immune changing patients, who suffered candidiasis, may be mismatched to the clinical treatment guidelines. The opinion could be shown by the literature of gastrointestinal system, and respiratory system, which suffered severely fungal infection mostly, mainly connected with the outside world and inside organ systems of the human body. They could show some patients have been excess treatment, and we should pay attention to the immune changing patients.展开更多
Poly(L-lactic acid)(PLLA)is a biocompatible,biodegradable material with piezoelectric properties,making it a promising candidate for providing self-powered stimulation to accelerate tissue re-pair.Repairs to various t...Poly(L-lactic acid)(PLLA)is a biocompatible,biodegradable material with piezoelectric properties,making it a promising candidate for providing self-powered stimulation to accelerate tissue re-pair.Repairs to various tissues,such as bone,cartilage and nerve,necessitate distinct piezoelectric characteristics even from the same material.However,the extensive utilization of PLLA piezoelectric scaffolds in various tissue is hindered by their low and single piezoelectric constants.In this study,PLLA nanofiber membranes with enhanced and adjustable piezoelectric constants(d_(33))were fabricated through oriented electrospinning.By carefully controlling the parameters of the spinning solution,a steady increase in d_(33) values from 0 to 30 pC/N was achieved.This advancement allows tailoring of PLLA nanofiber membranes to meet various piezoelectric requirements of different tissues.As an example of tailoring the optimal piezoelectric constants,we developed PLLA-2-0,PLLA-2-10,PLLA-2-15 and PLLA-2-20 nanofiber membranes with d_(33) values of 0,5,10 and 15 pC/N,respectively.The impact of varying piezoelectric constants of PLLA nanofiber membranes on cellular behavior and repair efficacy were validated through in vitro cellular experiments and in vivo mandibular critical defect repair.The results indicated that PLLA-2-20 demonstrated superior cell proliferation rate up to 130%and an osteogenic differentiation level approximately twice of the control.In addition,PLLA-2-20 significantly promoted cell adhesion and migration,and the cell aspect ratio was about five times higher than that of the control group.In vivo,PLLA-2-20 optimal restorative effects on rat mandibles via endogenous mechanical force-mediated piezoelectric stimulation,leading to complete histological restoration within 8 weeks.These findings highlight the potential of the PLLA membranes with high and adjustable d_(33) by a straightforward process.This study provides a novel approach for the development of highly electroactive membranes tailored to specific tissue repair needs.展开更多
Hemostatic materials are essential for managing acute bleeding in medical settings.Chitosan(CS)shows promise in hemostasis but its underlying mechanism remains incompletely understood.We unexpectedly discovered that c...Hemostatic materials are essential for managing acute bleeding in medical settings.Chitosan(CS)shows promise in hemostasis but its underlying mechanism remains incompletely understood.We unexpectedly discovered that certain protonated-chitosan(PCS)rapidly assembled plasma proteins to form protein membrane(PM)upon contact with platelet-poor plasma(PPP).We hypothesized that the novel observation was intricately related to the procoagulant effect of chitosan.Herein,the study aimed to elucidate the conditions necessary and mechanism for PM formation,identify the proteins within the PM and PCS's procoagulant action at the molecule levels.We confirmed that the amount of–NH3+groups(>4.9 mmol/g)on PCS molecules played a crucial role in promoting coagulation.The–NH3+group interacted with blood's multiple active components to exert hemostatic effects:assembling plasma proteins including coagulation factors such as FII,FV,FX,activating blood cells and promoting the secretion of coagulation-related substances(FV,ADP,etc)by platelets.Notably,the hemostatic mechanism can be extended to protonated-chitosan derivatives like quaternized,alkylated,and catechol-chitosan.In the blood clotting index(BCI)experiment,compared to other groups,PCS95 achieved the lowest BCI value(∼6%)within 30 s.Protonated-chitosan exhibited excellent biocompatibility and antibacterial properties,with PCS95 demonstrating inhibition effectiveness of over 95%against Escherichia coli(E.coil)and Staphylococcus aureus(S.aureus).Moreover,PCS performed enhanced hemostatic effectiveness over chitosan-based commercially agents(Celox™and ChitoGauze®XR)in diverse bleeding models.In particular,PCS95 reduced bleeding time by 70%in rabbit models of coagulopathy.Overall,this study investigated the coagulation mechanism of materials at the molecular level,paving the way for innovative approaches in designing new hemostatic materials.展开更多
In recent years,traditional Chinese medicine(TCM)has made great progress in the prevention and treatment of cancer.It has gradually revealed its characteristics and advantages in clinical practice,including alleviatin...In recent years,traditional Chinese medicine(TCM)has made great progress in the prevention and treatment of cancer.It has gradually revealed its characteristics and advantages in clinical practice,including alleviating clinical symptoms,prolonging survival time,decreasing the adverse effects of chemotherapy,and improving living quality.However,clinical TCM treatment of cancer lacks systematic theoretical guidance,because ancient TCM has not formed a recognized theoretical system of cognitive cancer,and there still are different opinions on the pathogenesis of cancer.Due to the complexity of cancer,the essence of cancer pathogenesis has not been described accurately by using common pathogenic factors,such as pathogenic wind,cold,dampness,summer heat,dryness,and fire.Ancient and modern TCM physicians have a similar understanding that the occurrence of cancer is related to toxin.In the 1990s,the thought of cancerous toxin was first proposed by Prof Zhou Zhongying,a TCMmaster based onmore than 60 years of clinical practice,who used“pandemic Qi(Li-Qi)is a specific pathogenic factor of epidemic disease”in Wenyi Lun(Treatise on Pestilence)for references.The pathogenesis theory of cancerous toxin was gradually established under the guidance of the thought of cancerous toxin.It holds that the cancerous toxin,a special pathogenic factor of cancer,is the key pathogenesis of the occurrence of malignant tumors.According to the pathogenesis theory of cancerous toxin,the basic pathogenesis of malignant tumors is the accumulation of pathogenic factors and cancerous toxin,and the deficiency of the vital Qi(Zheng-Qi).Therefore,the treatment principle involves eliminating pathogenic factors,resolving cancerous toxin,and supporting the vital Qi.The anticancer detoxification methods and the classification of Chinese medicinal herbs with anticancer detoxification effects were put forward.System theory has much in common with the concepts in the theory system of TCM,such as the universal relation theory,asking for a concrete analysis of concrete conditions,the humanism thought,and so on.This article aims to describe,review,and analyze the pathogenesis theory of cancerous toxin based on system theory for clinical practices.展开更多
Peripheral nervous system(PNS)injuries often lead to significant sensory and motor impairments.Traditional artificial nerve conduits,lacking anisotropic structures,have been associated with prolonged repair time and f...Peripheral nervous system(PNS)injuries often lead to significant sensory and motor impairments.Traditional artificial nerve conduits,lacking anisotropic structures,have been associated with prolonged repair time and failures in nerve regeneration.This study aimed to address these challenges by developing a novel approach for rapid repair of peripheral nerve injuries(PNI).A 3D oriented fibers scaffold featuring distinct radial(RFs)and longitudinal(LFs)fibers orientations was engineered using coaxial electrospinning and gas directional foaming techniques.This scaffold was then integrated with a shape memory conduit to form a directional multi-channel nerve conduit with micro/nanostructures.The results revealed that the grooved surface of the fibers significantly improved cellular directional guidance,effectively facilitating the migration of SCs from the periphery towards the center and from the base to the apex of the scaffold.In a rat model with a 10 mm nerve defect,the ND-PLATMC/LF ND-PCL scaffold significantly enhanced nerve regeneration and motor function recovery within 4 weeks.These results suggest the potential of this innovative scaffold for efficient repair of the nerve injuries.展开更多
Chimeric antigen receptor T-cell(CAR-T)therapy has greatly improved the disease remission rate and long-term survival rate of patients with relapsed/refractory hematological malignancies.[1-3]Currently,several commerc...Chimeric antigen receptor T-cell(CAR-T)therapy has greatly improved the disease remission rate and long-term survival rate of patients with relapsed/refractory hematological malignancies.[1-3]Currently,several commercial CAR-T products are available in the market and numerous CAR-T clinical trials have been conducted.Attention should be paid to the safety of CAR-T therapy.The main adverse effects of CAR-T therapy are cytokine release syndrome(CRS)and immune effector cell-associated neurotoxicity syndrome(ICANS).[4]展开更多
Osteoporosis is a reduction in skeletal mass due to the decrease of osteogenic ability and the activation of the osteoclastic function.Inhibiting bone resorption and accelerating the new bone formation is a promising ...Osteoporosis is a reduction in skeletal mass due to the decrease of osteogenic ability and the activation of the osteoclastic function.Inhibiting bone resorption and accelerating the new bone formation is a promising strategy to repair the bone defect of osteoporosis.In this study,we first systematically investigated the roles of Chinese medicine Asperosaponin Ⅵ(ASP Ⅵ)on osteogenic mineralization of BMSCs and osteoclastogenesis of BMMs,and then explored the synergistic effect of ASP Ⅵ and BS(BMP-2 immobilized in 2-N,6-O-sulfated chitosan)on bone formation.The result showed that ASP Ⅵ with the concentration lower than 10^(-4) M contributed to the expression of osteogenic gene and inhibited osteoclastic genes RANKL of BMSCs.Simultaneously,ASP Ⅵ significantly reduced the differentiation of mononuclear osteoclasts in the process of osteoclast formation induced by M-CSF and RANKL.Furthermore,by stimulating the SMADs,TGF-β1,VEGFA,and OPG/RANKL signaling pathways,ASBS(ASP Ⅵ and BS)substantially enhanced osteogenesis,greatly promoted angiogenesis,and suppressed osteoclastogenesis.The findings provide a new perspective on osteoporosis care and prevention.展开更多
基金supported by the‘Double First-Class’Construction Specialized Discipline Project at Zhejiang University(No HL2024012).
文摘Objectives:This systematic review and meta-analysis aimed to identify and synthesize the factors correlated with posttraumatic growth(PTG)in patients with colorectal cancer(CRC).Methods:PubMed,Web of Science,Embase,PsycINFO,CINAHL,Cochrane Library,China National Knowledge Infrastructure(CNKI),Wanfang database,China Science and Technology Journal Database(VIP)and SinoMed were searched for studies that reported data on the correlated factors associated with PTG in patients with CRC from inception to September 3,2024.The methodological quality of the included studies was assessed via the Agency for Healthcare Research and Quality(AHRQ)methodology checklist and the Newcastle-Ottawa Scale(NOS).Pearson correlation coefficient(r)was utilized to indicate effect size.Meta-analysis was conducted in R Studio.Results:Thirty-one eligible studies encompassing 6,400 participants were included in this review.Correlated factors were identified to be significantly associated with PTG in patients with CRC including demographic factors:residential area(r=0.13),marital status(r=0.10),employment status(r=0.18),education level(r=0.19),income level(r=0.16);disease-related factors:time since surgery(r=0.17),stoma-related complications(r=0.14),health-promoting behavior(r=0.46),and sexual function(r=0.17);psychosocial factors:confrontation coping(r=0.68),avoidance coping(r=-0.65),deliberate rumination(r=0.56),social support(r=0.47),family function(r=0.50),resilience(r=0.53),selfefficacy(r=0.91),self-compassion(r=-0.32),psychosocial adjustment(r=0.39),gratitude(r=0.45),stigma(r=-0.65),self-perceived burden(r=-0.31),fear of cancer recurrence(r=-0.45);and quality of life(r=0.32).Conclusions:This meta-analysis identified 23 factors associated with PTG in CRC patients.Medical workers can combine those relevant factors from the perspective of positive psychology,further explore the occurrence and development mechanism of PTG,and establish targeted interventions to promote PTG.
基金Supported by the Pioneer and Leading Goose R&D Program of Zhejiang(No.2023C03130)the National Key R&D Program of China(No.2019YFD0901101)+4 种基金the National Natural Science Foundation of China(No.42076169)the Oceanic Interdisciplinary Program of Shanghai Jiao Tong University(Nos.SL2022ZD203,SL2022MS012)the Zhejiang Provincial Natural Science Founds for Distinguished Young Scientists(No.LR21D060001)the State Key Laboratory of Satellite Ocean Environment Dynamics(No.SOEDZZ1902)the ChinaAPEC Cooperation Fund(No.2029901)。
文摘Microplastics(MPs)have garnered significant international scrutiny as an emerging environmental pollutant,constituting one of the four principal global environmental threats and posing potential health hazards to humans.However,data on the impact of MPs on the early life of the commercially important fish remain limited.In this study,polystyrene microspheres(PS-MPs)(1 and 5μm)were used to investigate the effects of MPs on the growth,development,and metabolism in early life stages of large yellow croaker Pseudosciaena crocea.Results indicate that MPs were enriched in the gastrointestinal tract and gills of the fish.In addition,PS-MPs(1μm)exhibited no obvious effects on embryo hatching and heart rates,while increased the mortality rate(23.00%vs.control 14.99%)and decreased the body length(4098.61±447.03μm vs.control with 2827.04±254.75μm)of the larvae at the highest exposure concentration(5×10^(4)items/L).Metabolomics analysis revealed that PS-MPs(5μm)induced mild perturbations in phospholipid metabolism,specifically alterations in phosphatidylethanolamine(PE)levels.These changes influenced the cell membranes of juvenile fish,and consequently elicited inflammatory responses,disrupted lipid homeostasis,and affected other critical physiological processes.Ultimately,these effects may avoid the growth retardation and potential mortality.Therefore,PS-MPs could affect negatively the fish health in the early life stage,which has implications for aquatic ecosystems.
文摘Objective： The purposes of this study were to assess the efficacy of allogeneic hematopoietic stem cell transplantation （HSCT） for acute leukemia （AL） and analyze the factors affecting the prognosis of these patients. Methods： The clinical and follow-up data of 93 AL patients （median age, 30 years） undergoing allogeneic HSCT in Xiangya Hospital over the past 12 years were collected, and the potential factors affecting the efficacy and prognosis of allogeneic HSCT patients were determined. Results： Hematopoietic reconstitution was achieved in 90 patients. At the last follow-up, the incidences of severe acute graft versus host disease （aGvHD） and extensive chronic GvHD （cGvHD） were 14.0% and 20.0%, the 3-year cumulative incidence of transplantation related mortality （TRM） and relapse rate were 16.8%±6.1% and 21.3%±6.7%, and the estimated 3-year overall survival （OS） and disease-free survival （DFS） of the patients were 64.6%±5.4% and 56.5%±5.5%, respectively. Univariate analysis indicated that age older than 40 years, HLA mismatch, and severe lung infection within the first 100 days after transplantation were risk factors for severe aGvHD, age older than 40 years, HLA mismatch, severe lung infection within the first 100 days after transplantation, and severe aGvHD were risk factors for TRM, high-risk AL and lack of cGvHD were risk factors for relapse （all P〈0.05）. Survival estimation showed that HLA mismatch, severe lung infection occurring within the first 100 days post-transplantation, high-risk AL severe aGvHD and lack of cGvHD were risk factors associated with poor prognosis （all P〈0.05）. Further multivariate analyses revealed that severe lung infection within the first 100 days post-transplantation, severe aGvHD and lack of cGvHD were independent risk factors for unfavorable outcomes （all P〈0.05）. Conclusions： Allogeneic HSCT can improve the DFS of AL patients, and severe lung infection within the first 100 days post-transplantation, severe aGvHD and lack of cGvHD are independent risk factors affecting the prognosis.
文摘The guideline of the Infectious Diseases Society of America (IDSA) about candidiasis is a key standard for clinical doctors to treat patients, and textbook to teach medical students. However, in the progress of clinical treatment and documents checking, the immune changing patients, who suffered candidiasis, may be mismatched to the clinical treatment guidelines. The opinion could be shown by the literature of gastrointestinal system, and respiratory system, which suffered severely fungal infection mostly, mainly connected with the outside world and inside organ systems of the human body. They could show some patients have been excess treatment, and we should pay attention to the immune changing patients.
基金supported by the National Natural Science Foundation of China(32171342,32471407)the National Key Research and Development Program of China(2023YFC2413600,2022YFB3804303)the Joint Fund for Equipment Pre-research of the Ministry of Education(6141A02022618).
文摘Poly(L-lactic acid)(PLLA)is a biocompatible,biodegradable material with piezoelectric properties,making it a promising candidate for providing self-powered stimulation to accelerate tissue re-pair.Repairs to various tissues,such as bone,cartilage and nerve,necessitate distinct piezoelectric characteristics even from the same material.However,the extensive utilization of PLLA piezoelectric scaffolds in various tissue is hindered by their low and single piezoelectric constants.In this study,PLLA nanofiber membranes with enhanced and adjustable piezoelectric constants(d_(33))were fabricated through oriented electrospinning.By carefully controlling the parameters of the spinning solution,a steady increase in d_(33) values from 0 to 30 pC/N was achieved.This advancement allows tailoring of PLLA nanofiber membranes to meet various piezoelectric requirements of different tissues.As an example of tailoring the optimal piezoelectric constants,we developed PLLA-2-0,PLLA-2-10,PLLA-2-15 and PLLA-2-20 nanofiber membranes with d_(33) values of 0,5,10 and 15 pC/N,respectively.The impact of varying piezoelectric constants of PLLA nanofiber membranes on cellular behavior and repair efficacy were validated through in vitro cellular experiments and in vivo mandibular critical defect repair.The results indicated that PLLA-2-20 demonstrated superior cell proliferation rate up to 130%and an osteogenic differentiation level approximately twice of the control.In addition,PLLA-2-20 significantly promoted cell adhesion and migration,and the cell aspect ratio was about five times higher than that of the control group.In vivo,PLLA-2-20 optimal restorative effects on rat mandibles via endogenous mechanical force-mediated piezoelectric stimulation,leading to complete histological restoration within 8 weeks.These findings highlight the potential of the PLLA membranes with high and adjustable d_(33) by a straightforward process.This study provides a novel approach for the development of highly electroactive membranes tailored to specific tissue repair needs.
基金National Natural Science Foundation of China(No.3217134251772100)National Key Research and Development Program of China(2022YFB3804300).
文摘Hemostatic materials are essential for managing acute bleeding in medical settings.Chitosan(CS)shows promise in hemostasis but its underlying mechanism remains incompletely understood.We unexpectedly discovered that certain protonated-chitosan(PCS)rapidly assembled plasma proteins to form protein membrane(PM)upon contact with platelet-poor plasma(PPP).We hypothesized that the novel observation was intricately related to the procoagulant effect of chitosan.Herein,the study aimed to elucidate the conditions necessary and mechanism for PM formation,identify the proteins within the PM and PCS's procoagulant action at the molecule levels.We confirmed that the amount of–NH3+groups(>4.9 mmol/g)on PCS molecules played a crucial role in promoting coagulation.The–NH3+group interacted with blood's multiple active components to exert hemostatic effects:assembling plasma proteins including coagulation factors such as FII,FV,FX,activating blood cells and promoting the secretion of coagulation-related substances(FV,ADP,etc)by platelets.Notably,the hemostatic mechanism can be extended to protonated-chitosan derivatives like quaternized,alkylated,and catechol-chitosan.In the blood clotting index(BCI)experiment,compared to other groups,PCS95 achieved the lowest BCI value(∼6%)within 30 s.Protonated-chitosan exhibited excellent biocompatibility and antibacterial properties,with PCS95 demonstrating inhibition effectiveness of over 95%against Escherichia coli(E.coil)and Staphylococcus aureus(S.aureus).Moreover,PCS performed enhanced hemostatic effectiveness over chitosan-based commercially agents(Celox™and ChitoGauze®XR)in diverse bleeding models.In particular,PCS95 reduced bleeding time by 70%in rabbit models of coagulopathy.Overall,this study investigated the coagulation mechanism of materials at the molecular level,paving the way for innovative approaches in designing new hemostatic materials.
基金supported by the National Natural Science Foundation of China(No.81973737)National Key R&D Program of China(No.2022YFC3500200 and 2022YFC3500201)+2 种基金Innovation Team and Talents Cultivation Program of National Administration of TraditionalChinese Medicine(No.ZYYCXTD-C-202208)NATCM’s Project of High-level Construction of Key TCM Disciplines,Qing Lan Project of Jiangsu Higher Education Institutions,Jiangsu Postgraduate Practice Innovation Plan(No.SJCX22_0706)General Project of Universities’Philosophy and Social Science in Jiangsu Province.
文摘In recent years,traditional Chinese medicine(TCM)has made great progress in the prevention and treatment of cancer.It has gradually revealed its characteristics and advantages in clinical practice,including alleviating clinical symptoms,prolonging survival time,decreasing the adverse effects of chemotherapy,and improving living quality.However,clinical TCM treatment of cancer lacks systematic theoretical guidance,because ancient TCM has not formed a recognized theoretical system of cognitive cancer,and there still are different opinions on the pathogenesis of cancer.Due to the complexity of cancer,the essence of cancer pathogenesis has not been described accurately by using common pathogenic factors,such as pathogenic wind,cold,dampness,summer heat,dryness,and fire.Ancient and modern TCM physicians have a similar understanding that the occurrence of cancer is related to toxin.In the 1990s,the thought of cancerous toxin was first proposed by Prof Zhou Zhongying,a TCMmaster based onmore than 60 years of clinical practice,who used“pandemic Qi(Li-Qi)is a specific pathogenic factor of epidemic disease”in Wenyi Lun(Treatise on Pestilence)for references.The pathogenesis theory of cancerous toxin was gradually established under the guidance of the thought of cancerous toxin.It holds that the cancerous toxin,a special pathogenic factor of cancer,is the key pathogenesis of the occurrence of malignant tumors.According to the pathogenesis theory of cancerous toxin,the basic pathogenesis of malignant tumors is the accumulation of pathogenic factors and cancerous toxin,and the deficiency of the vital Qi(Zheng-Qi).Therefore,the treatment principle involves eliminating pathogenic factors,resolving cancerous toxin,and supporting the vital Qi.The anticancer detoxification methods and the classification of Chinese medicinal herbs with anticancer detoxification effects were put forward.System theory has much in common with the concepts in the theory system of TCM,such as the universal relation theory,asking for a concrete analysis of concrete conditions,the humanism thought,and so on.This article aims to describe,review,and analyze the pathogenesis theory of cancerous toxin based on system theory for clinical practices.
基金National Natural Science Foundation of China(No.3217134251772100)National Key Research and Development Program of China(2023YFC2413600)。
文摘Peripheral nervous system(PNS)injuries often lead to significant sensory and motor impairments.Traditional artificial nerve conduits,lacking anisotropic structures,have been associated with prolonged repair time and failures in nerve regeneration.This study aimed to address these challenges by developing a novel approach for rapid repair of peripheral nerve injuries(PNI).A 3D oriented fibers scaffold featuring distinct radial(RFs)and longitudinal(LFs)fibers orientations was engineered using coaxial electrospinning and gas directional foaming techniques.This scaffold was then integrated with a shape memory conduit to form a directional multi-channel nerve conduit with micro/nanostructures.The results revealed that the grooved surface of the fibers significantly improved cellular directional guidance,effectively facilitating the migration of SCs from the periphery towards the center and from the base to the apex of the scaffold.In a rat model with a 10 mm nerve defect,the ND-PLATMC/LF ND-PCL scaffold significantly enhanced nerve regeneration and motor function recovery within 4 weeks.These results suggest the potential of this innovative scaffold for efficient repair of the nerve injuries.
文摘Chimeric antigen receptor T-cell(CAR-T)therapy has greatly improved the disease remission rate and long-term survival rate of patients with relapsed/refractory hematological malignancies.[1-3]Currently,several commercial CAR-T products are available in the market and numerous CAR-T clinical trials have been conducted.Attention should be paid to the safety of CAR-T therapy.The main adverse effects of CAR-T therapy are cytokine release syndrome(CRS)and immune effector cell-associated neurotoxicity syndrome(ICANS).[4]
基金funded by National Key Research and Development Program of China(No.2016YFC1102900)National Natural Science Foundation of China(No.51772100 and No.32171342)+2 种基金Shanghai Science and Technology Agriculture Project(No.202002080002F01474)Shanghai Pujiang Program(16PJD015)Joint Fund for equipment pre-research of the ministry of education(6141A02022618).
文摘Osteoporosis is a reduction in skeletal mass due to the decrease of osteogenic ability and the activation of the osteoclastic function.Inhibiting bone resorption and accelerating the new bone formation is a promising strategy to repair the bone defect of osteoporosis.In this study,we first systematically investigated the roles of Chinese medicine Asperosaponin Ⅵ(ASP Ⅵ)on osteogenic mineralization of BMSCs and osteoclastogenesis of BMMs,and then explored the synergistic effect of ASP Ⅵ and BS(BMP-2 immobilized in 2-N,6-O-sulfated chitosan)on bone formation.The result showed that ASP Ⅵ with the concentration lower than 10^(-4) M contributed to the expression of osteogenic gene and inhibited osteoclastic genes RANKL of BMSCs.Simultaneously,ASP Ⅵ significantly reduced the differentiation of mononuclear osteoclasts in the process of osteoclast formation induced by M-CSF and RANKL.Furthermore,by stimulating the SMADs,TGF-β1,VEGFA,and OPG/RANKL signaling pathways,ASBS(ASP Ⅵ and BS)substantially enhanced osteogenesis,greatly promoted angiogenesis,and suppressed osteoclastogenesis.The findings provide a new perspective on osteoporosis care and prevention.
