The emergence of fluorescent light-up molecular probe,which can specifically turn on their fluorescent in the presence of stimulation factors,has open up a new opportunity to advance biosensing and bioimaging.In this ...The emergence of fluorescent light-up molecular probe,which can specifically turn on their fluorescent in the presence of stimulation factors,has open up a new opportunity to advance biosensing and bioimaging.In this work,we designed and synthesized a peptide-AIE conjugate probe for cell imaging with controlled in situ assembled nanostructures.The modular designed probe is consisted of a selfassembled peptide-tetraphenylethene(TPE)motif,a fibroblast activation protein alpha(FAP-α)responsive motif,a hydrophilic motif and a targeting motif.The probe exhibits typically turn-on fluorescence property specifically triggered by FAP-α,which is a significant overexpressed membrane protein on pancreatic tumor cells.Interestingly,the peptide modified the TPE dramatically impacts the assembled nanostructure,which can be modulated by peptide sequences.As a result,the peptide FF(PhePhe)modification of TPE as the self-assembled motif provides a suitable balance of the probe with lightup property and nanofiber assembled structure in situ.Finally,our probe could effectively detect the FAP-αon tumor cells with high specificity.Meantime,the nanofibers in situ assembled on the surface of CAFs enhanced the probe accumulation and prolonged the retention for cell imaging.We envision that this study may inspire new insights into the design of nanostructure controlled AIE light-up bio-probe.展开更多
Achyranthes bidentata polypeptides（ABPP） have been reported to inhibit apoptosis of retinal ganglion cells（RGCs）.The present study investigated the protective effects of ABPP on RGCs in a rat model of optic nerve ...Achyranthes bidentata polypeptides（ABPP） have been reported to inhibit apoptosis of retinal ganglion cells（RGCs）.The present study investigated the protective effects of ABPP on RGCs in a rat model of optic nerve injury.With prolonged injury time,RGC densities were gradually decreased.ABPP（5 μg） significantly increased RGC densities and upregulated growth associated protein 43 expression in rats with optic nerve injury.Results demonstrate that ABPP can protect RGCs and promote axonal growth after optic nerve crush.展开更多
基金supported by National Key R&D Program of China(No.2018YFE0205400)National Natural Science Foundation of China(Nos.31671028 and 51873045)Youth Innovation Promotion CAS(No.2017053)。
文摘The emergence of fluorescent light-up molecular probe,which can specifically turn on their fluorescent in the presence of stimulation factors,has open up a new opportunity to advance biosensing and bioimaging.In this work,we designed and synthesized a peptide-AIE conjugate probe for cell imaging with controlled in situ assembled nanostructures.The modular designed probe is consisted of a selfassembled peptide-tetraphenylethene(TPE)motif,a fibroblast activation protein alpha(FAP-α)responsive motif,a hydrophilic motif and a targeting motif.The probe exhibits typically turn-on fluorescence property specifically triggered by FAP-α,which is a significant overexpressed membrane protein on pancreatic tumor cells.Interestingly,the peptide modified the TPE dramatically impacts the assembled nanostructure,which can be modulated by peptide sequences.As a result,the peptide FF(PhePhe)modification of TPE as the self-assembled motif provides a suitable balance of the probe with lightup property and nanofiber assembled structure in situ.Finally,our probe could effectively detect the FAP-αon tumor cells with high specificity.Meantime,the nanofibers in situ assembled on the surface of CAFs enhanced the probe accumulation and prolonged the retention for cell imaging.We envision that this study may inspire new insights into the design of nanostructure controlled AIE light-up bio-probe.
基金the Priority Academic Program Development of Jiangsu Higher Education Institutions (PADA)
文摘Achyranthes bidentata polypeptides（ABPP） have been reported to inhibit apoptosis of retinal ganglion cells（RGCs）.The present study investigated the protective effects of ABPP on RGCs in a rat model of optic nerve injury.With prolonged injury time,RGC densities were gradually decreased.ABPP（5 μg） significantly increased RGC densities and upregulated growth associated protein 43 expression in rats with optic nerve injury.Results demonstrate that ABPP can protect RGCs and promote axonal growth after optic nerve crush.
