Purpose: We investigated the efficacy of potent or combined antibiotics in patients suffering bacterial infections secondary to H1N1 by retrospectively analyzing their bacterial pathogen spectrum and clinical characte...Purpose: We investigated the efficacy of potent or combined antibiotics in patients suffering bacterial infections secondary to H1N1 by retrospectively analyzing their bacterial pathogen spectrum and clinical characteristics. Methods: Multi-center retrospective analysis was performed using clinical data of H1N1 patients from 27 hospitals in Hebei Province, China, from November 1 to December 31, 2009. Results: Of 480 H1N1-infected patients enrolled from an inpatient clinic, 91 were positive for bacterial culture. Bacteria were detected in sputum culture at 7.00 ± 8.87 days post-admission. Compared with the negative group, the patients in the positive sputum culture group had a higher mean age and prevalence of basic diseases, higher APECHEII (Acute Physiology and Chronic Health Evaluation II) score within 24 hours of admission, longer hospital stays, and higher mortality. In total, 189 bacterial strains were isolated, with the majority of samples testing positive for Acinetobacter baumanii (47), Streptococcus viridians (26), or Pseudomonas aeruginosa (19). S. viridians was the major cause of infection within 3 days of admission, while A. baumanii infection was more prevalent from 4 days post-admission;there was a significant difference in the constituent ratio between the two pathogens (p 0.001). Compared with patients administered common antibiotics, the potent antibiotics group showed no significant difference in hospitalization time, time until bacterial detection, mortality, or detection ratio of resistant strains (p > 0.05). Conclusions: Complicated bacterial infection in H1N1 patients increases hospitalization time and mortality. Gram-negative bacilli and multi-resistant strains are the main sources of infection. Early administration of potent or combined antibiotics, even during the period of rapid onset, may not be suitable in H1N1-infected patients, particularly previously healthy young patients.展开更多
The functional development of the mammalian lung is a complex process that relies on the spatial and temporal organization of multiple cell types and their states.However,a comprehensive spatiotemporal transcriptome a...The functional development of the mammalian lung is a complex process that relies on the spatial and temporal organization of multiple cell types and their states.However,a comprehensive spatiotemporal transcriptome atlas of the developing lung has not yet been reported.Here we apply high-throughput spatial transcriptomics to allow for a comprehensive assessment of mouse lung development comprised of two critical developmental events:branching morphogenesis and alveologenesis.We firstly generate a spatial molecular atlas of mouse lung development spanning from E12.5 to P0 based on the integration of published single cell RNA-sequencing data and identify 10 spatial domains critical for functional lung organization.Furthermore,we create a lineage trajectory connecting spatial clusters from adjacent time points in E12.5–P0 lungs and explore TF(transcription factor)regulatory networks for each lineage specification.We observe the establishment of pulmonary airways within the developing lung,accompanied by the proximal–distal patterning with distinct characteristics of gene expression,signaling landscape and transcription factors enrichment.We characterize the alveolar niche heterogeneity with maturation state differences during the later developmental stage around birth and demonstrate differentially expressed genes,such as Angpt2 and Epha3,which may perform a critical role during alveologenesis.In addition,multiple signaling pathways,including ANGPT,VEGF and EPHA,exhibit increased levels in more maturing alveolar niche.Collectively,by integrating the spatial transcriptome with corresponding single-cell transcriptome data,we provide a comprehensive molecular atlas of mouse lung development with detailed molecular domain annotation and communication,which would pave the way for understanding human lung development and respiratory regeneration medicine.展开更多
The water content in vegetative leaves is an important indicator to plant science.It reveals the physiological status of plants and provides valuable information in irrigation management.Terahertz(THz)as a state-of-th...The water content in vegetative leaves is an important indicator to plant science.It reveals the physiological status of plants and provides valuable information in irrigation management.Terahertz(THz)as a state-of-the-art technology shows great potential in measuring and monitoring the water status in plant leaves.This paper reviewed the theoretical models for calculating water content in the plant leaves,the methods for eliminating the scattering loss caused by the surface roughness of leaf,the applications of THz spectroscopy and THz imaging for monitoring leaf water content and describing leaf water distribution.The survey of the researches presents the considerable advantages of this emerging and promising THz technology in agriculture.展开更多
文摘Purpose: We investigated the efficacy of potent or combined antibiotics in patients suffering bacterial infections secondary to H1N1 by retrospectively analyzing their bacterial pathogen spectrum and clinical characteristics. Methods: Multi-center retrospective analysis was performed using clinical data of H1N1 patients from 27 hospitals in Hebei Province, China, from November 1 to December 31, 2009. Results: Of 480 H1N1-infected patients enrolled from an inpatient clinic, 91 were positive for bacterial culture. Bacteria were detected in sputum culture at 7.00 ± 8.87 days post-admission. Compared with the negative group, the patients in the positive sputum culture group had a higher mean age and prevalence of basic diseases, higher APECHEII (Acute Physiology and Chronic Health Evaluation II) score within 24 hours of admission, longer hospital stays, and higher mortality. In total, 189 bacterial strains were isolated, with the majority of samples testing positive for Acinetobacter baumanii (47), Streptococcus viridians (26), or Pseudomonas aeruginosa (19). S. viridians was the major cause of infection within 3 days of admission, while A. baumanii infection was more prevalent from 4 days post-admission;there was a significant difference in the constituent ratio between the two pathogens (p 0.001). Compared with patients administered common antibiotics, the potent antibiotics group showed no significant difference in hospitalization time, time until bacterial detection, mortality, or detection ratio of resistant strains (p > 0.05). Conclusions: Complicated bacterial infection in H1N1 patients increases hospitalization time and mortality. Gram-negative bacilli and multi-resistant strains are the main sources of infection. Early administration of potent or combined antibiotics, even during the period of rapid onset, may not be suitable in H1N1-infected patients, particularly previously healthy young patients.
基金supported in part by the National Key R&D Program of China(2024YFC3405602 and 2024YFF0509000)National Natural Science Foundation of China(32270854,32161160322,32370972,and 32100483)+5 种基金Guangdong Basic and Applied Basic Research Foundation(2024B1515040020,2024B1515020052,2023A1515011783,and 2019B151502054)Science and Technology Planning Project of Guangdong Province(2023B1212060050 and 2023B1212120009)Basic Research Project of Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences(GIBHBRP23-01)the Union Project from Guangzhou National Laboratory and State Key Laboratory of Respiratory Disease,Guangzhou Medical University(GZNL2024B01004)Major Project of Guangzhou National Laboratory(GZNL2023A03005 and GZNL2023A02007)the Research Funds from Health@InnoHK Program launched by Innovation Technology Commission of the Hong Kong SAR,P.R.China。
文摘The functional development of the mammalian lung is a complex process that relies on the spatial and temporal organization of multiple cell types and their states.However,a comprehensive spatiotemporal transcriptome atlas of the developing lung has not yet been reported.Here we apply high-throughput spatial transcriptomics to allow for a comprehensive assessment of mouse lung development comprised of two critical developmental events:branching morphogenesis and alveologenesis.We firstly generate a spatial molecular atlas of mouse lung development spanning from E12.5 to P0 based on the integration of published single cell RNA-sequencing data and identify 10 spatial domains critical for functional lung organization.Furthermore,we create a lineage trajectory connecting spatial clusters from adjacent time points in E12.5–P0 lungs and explore TF(transcription factor)regulatory networks for each lineage specification.We observe the establishment of pulmonary airways within the developing lung,accompanied by the proximal–distal patterning with distinct characteristics of gene expression,signaling landscape and transcription factors enrichment.We characterize the alveolar niche heterogeneity with maturation state differences during the later developmental stage around birth and demonstrate differentially expressed genes,such as Angpt2 and Epha3,which may perform a critical role during alveologenesis.In addition,multiple signaling pathways,including ANGPT,VEGF and EPHA,exhibit increased levels in more maturing alveolar niche.Collectively,by integrating the spatial transcriptome with corresponding single-cell transcriptome data,we provide a comprehensive molecular atlas of mouse lung development with detailed molecular domain annotation and communication,which would pave the way for understanding human lung development and respiratory regeneration medicine.
基金This work was supported by the National Key Point Research and Invention Program of the Thirteenth(2016YFD0700304)the National Key Research&Development program of China(2016YFD0300606 and 2017YFD0700501).
文摘The water content in vegetative leaves is an important indicator to plant science.It reveals the physiological status of plants and provides valuable information in irrigation management.Terahertz(THz)as a state-of-the-art technology shows great potential in measuring and monitoring the water status in plant leaves.This paper reviewed the theoretical models for calculating water content in the plant leaves,the methods for eliminating the scattering loss caused by the surface roughness of leaf,the applications of THz spectroscopy and THz imaging for monitoring leaf water content and describing leaf water distribution.The survey of the researches presents the considerable advantages of this emerging and promising THz technology in agriculture.
