AIM: Loss of heterozygosity (LOH) on tumor suppressor genes is believed to play a key role in carcinogenesis of colorectal cancer. When it occurs at a tumor suppressor gene locus with abnormal allele, neoplastic trans...AIM: Loss of heterozygosity (LOH) on tumor suppressor genes is believed to play a key role in carcinogenesis of colorectal cancer. When it occurs at a tumor suppressor gene locus with abnormal allele, neoplastic transformation happens. In this study, we analyzed the LOH at 21 loci on chromosome 1 in sporadic colorectal cancer to identify additional loci involved in colorectal tumorigenesis.METHODS: Twenty-one polymorphic micro-satellite DNA markers were analyzed with PCR both in 83 cases of colorectal cancer and in normal tissues. PCR products were eletrophoresed on an ABI 377 DNA sequencer. Genescan 3.1 and Genotype 2.1 software were used for LOH scanning and analysis. X^2 test was used to compare LOH frequency with clinicopathological data. P<0.05 was considered as statistically significant.RESULTS: The average LOH frequency of chromosome 1,short arm and long arm was 19.83%, 18.00% and 21.66%,respectively. The 2 highest LOH loci with a frequency of 36.54% and 32.50% were identified on DIS468 (1p36.33-p36.31) and DIS413 (1q31.3), respectively. On DIS2726 locus, LOH frequency of rectal cancer was 28.57% (6/21),which was higher than that of colon cancer (0.00%, 0/33) (P=0.002), suggesting that the mechanism of carcinogenisis was different in both groups.CONCLUSION: Putative tumor suppressor genes on chromosome 1 may relate to sporadic colorectal carcinomas.Tumor-suppressor-genes might locate on 1p36.33-36.31and/or 1q31.3.展开更多
The bleaching of indigo-carmine catalyzed by laccase was monitored on-line by UV/Vis spectroscopy. The resulted data were analyzed by evolving factor analysis (EFA) and multivariate curve resolution (MCR), and the re...The bleaching of indigo-carmine catalyzed by laccase was monitored on-line by UV/Vis spectroscopy. The resulted data were analyzed by evolving factor analysis (EFA) and multivariate curve resolution (MCR), and the reaction mechanism was proposed.展开更多
基金Supported by the National Natural Science Foundation of China,No.30080016
文摘AIM: Loss of heterozygosity (LOH) on tumor suppressor genes is believed to play a key role in carcinogenesis of colorectal cancer. When it occurs at a tumor suppressor gene locus with abnormal allele, neoplastic transformation happens. In this study, we analyzed the LOH at 21 loci on chromosome 1 in sporadic colorectal cancer to identify additional loci involved in colorectal tumorigenesis.METHODS: Twenty-one polymorphic micro-satellite DNA markers were analyzed with PCR both in 83 cases of colorectal cancer and in normal tissues. PCR products were eletrophoresed on an ABI 377 DNA sequencer. Genescan 3.1 and Genotype 2.1 software were used for LOH scanning and analysis. X^2 test was used to compare LOH frequency with clinicopathological data. P<0.05 was considered as statistically significant.RESULTS: The average LOH frequency of chromosome 1,short arm and long arm was 19.83%, 18.00% and 21.66%,respectively. The 2 highest LOH loci with a frequency of 36.54% and 32.50% were identified on DIS468 (1p36.33-p36.31) and DIS413 (1q31.3), respectively. On DIS2726 locus, LOH frequency of rectal cancer was 28.57% (6/21),which was higher than that of colon cancer (0.00%, 0/33) (P=0.002), suggesting that the mechanism of carcinogenisis was different in both groups.CONCLUSION: Putative tumor suppressor genes on chromosome 1 may relate to sporadic colorectal carcinomas.Tumor-suppressor-genes might locate on 1p36.33-36.31and/or 1q31.3.
基金The work was supported by the National Natural Science Foundation of China[Grant No.20075021]visiting grant of Reutlingen University of Applied Sciences in Germany.
文摘The bleaching of indigo-carmine catalyzed by laccase was monitored on-line by UV/Vis spectroscopy. The resulted data were analyzed by evolving factor analysis (EFA) and multivariate curve resolution (MCR), and the reaction mechanism was proposed.
